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8/12/2019 Bsl1 Template

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General Laboratory Practices for BSL-1 LaboratoriesTemplate (Revised 10/200 !

XXX Lab "ote#T$is is a %eneral template for laboratory safety proced&res' T$e principalinvesti%ator is responsible for incl&din% laboratory-specific proced&res foraddressin% $a ards in t$eir laboratory' )nyt$in% can be added to t$istemplate* b&t at a minim&m incl&de information +$ere yo& see italics.

, $ave read* &nderstand* and a%ree to ad$ere to t$e biosafety proced&res contained +it$in#

Printed "ame Si%nat&re ateP,#

................ ................ .......

Staff# ................ ................ ....... ................ ................ ....... ................ ................ ....... ................ ................ ....... ................ ................ ....... ................ ................ ....... ................ ................ ....... ................ ................ ....... ................ ................ .......

ritten ( ate!# ............... Last Revision ( ate!# ............ Revie+ed by BS ( ate!# ............ )dopted by t$e ,B ( ate!# ............

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Table of Contents

Principal ,nvesti%ator Responsibilities ''' 3Laboratory Staff/St&dent Responsibilities ''''' 3

General 4mer%ency ,nformation ' ''' 5Reportable r ") incidents 56ire )larms/47tin%&is$ers ''' ' '' 54ye+as$es ' ' 8Spills in t$e Laboratory ' ' 8Biolo%ical Spill ' '' 8Spills &tside t$e Biosafety abinet ' ' 8Spills ,nside t$e Biosafety abinet '' '' 8Spills ,nside 49&ipment ' ' 8

$emical Spill ' :Radiation Spill ' ''' :

47pos&res in t$e Laboratory ' ' : "e+ 4mployees/;isitors ' 'Trainin% ' ' 'PP4 ' ' '' ' in% in afres$ 10H bleac$ sol&tion or disinfectant for at least 20 min&tes'

lean area at least 2 feet aro&nd spill +it$ disinfectant' (,ncl&din% f&rnit&re/+alls!

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Spills Inside t&e )iosafety Cabinet

econtaminate any biolo%ical spill by coverin% t$e spill +it$ paper to+els and soa>in% indisinfectant for at least 20 min&tes'

$ile spill is soa>in%* +ipe do+n all +or> s&rfaces and e9&ipment in BS +it$disinfectant' Place all clean&p material into bio$a ardo&s +aste container'

Spills Inside E+uipment

,f t$e spill occ&rs in a s$a>er or centrif&%e* t&rn off t$e e9&ipment and leave t$e doorclosed for at least 30 min&tes to red&ce aerosol e7pos&re before cleanin% &p t$e spill'

Tape a si%n indicatin% t$e problem and yo&r name to t$e contaminated e9&ipment &ntilt$e sit&ation $as been rectified'

C&emical Spill

Location of spill 'it #

lean &p all c$emical spills immediately'

,f yo& spill a $a ardo&s material and need assistance* call 4@S'

Radiation Spill

Location of spill 'it$

ipe &p t$e spill +it$ Lift-a+ay and c$ec> for resid&al contamination by performin% a+ipe test (triti&m or carbon-15! of t$e affected area'

6or ot$er radioisotopes* refer to t$e 4@S Radiation Safety ?an&al for proper detectionmet$ods'

all 4@S if yo& need assistance'

E%posures in t&e Laboratory

)ll e7pos&res m&st be reported to t$e P, and to 4@S'

)iological E%posure

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Remove any contaminated clot$in% and +as$ s>in e7posed to t$e a%ent +it$ adisinfectant s&c$ as antibacterial soap'

)&toclave any contaminated clot$in% before disposal' econtaminate any s&rfaces &sin%t$e proced&re for a bio$a ardo&s spill'

,f yo& believe yo& may $ave been e7posed to an a%ent* contact 4@S and see> immediatemedical attention'

,f yo& s&spect yo& $ave a lab-ac9&ired illness re%ardless of a recent e7pos&re* see a$ealt$ care professional immediately' Provide t$e $ealt$ care professional +it$ 4@Scontact information to allo+ for coordination +it$ t$e &niversity safety office'

6ill o&t a +or>erIs compensation form even if yo& are not s&re if yo&r illness +asac9&ired at +or>' T$is m&st be done if yo& need to file a claim later'

C&emical E%posure

6or most c$emical e7pos&res to t$e s>in* +as$ t$e c$emical off +it$ +ater for at least 18min&tes and remove any contaminated clot$in%' Cse t$e sin> for small e7pos&resJ forlar%e e7pos&res &se t$e safety s$o+er'

Location of safet" sho&er$

Radiation E%posure

6or s>in contamination +it$ radioactivity* +as$ it off and remove any contaminatedclot$in%' ,f yo& believe yo& $ave in$aled or in%ested any radioactivity* call 4@S'

!e' Employees/,isitors

Location of this lab anual$

Laboratory personnel s$o&ld be a+are of t$e potential $a ards associated +it$ t$e +or>and be proficient in t$e specified practices and proced&res'

no+ t$e c$emicals yo& are +or>in% +it$' Loo> &p t$e material safety data s$eet(?S S! available at $ttp#//+++'&te7as'ed&/safety/e$s/msds/inde7'$tml ' Place a

boo>mar> on yo&r comp&ter so t$at yo& can access t$is information 9&ic>ly'

6or biolo%ical a%ents* see $ttp#//+++'p$ac-aspc'%c'ca/msds-ftss/inde7'$tml '

,f yo& are &sin% a piece of e9&ipment for t$e first time* be s&re to as> for instr&ctions'

Training

)ll laboratory researc$ personnel m&st ta>e instit&tional provided trainin%' Trainin%m&st be doc&mented (electronic or paper!' Personnel s$o&ld not initiate researc$ &ntiltrainin% is completed'
http://www.utexas.edu/safety/ehs/msds/index.htmlhttp://www.phac-aspc.gc.ca/msds-ftss/index.htmlhttp://www.utexas.edu/safety/ehs/msds/index.htmlhttp://www.phac-aspc.gc.ca/msds-ftss/index.html


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lasses#

@101# General @a ard omm&nication

@102# Site-Specific @a ard omm&nication (provided by t$e P,!

@201# General Laboratory Safety

@202# @a ardo&s aste ?ana%ement

66208# 6ire 47tin%&is$er Trainin%

@20 # Biolo%ical Safety

812 ( nline!# ,B Trainin%# ",@ G&idelines

)dditionally if researc$ers are +or>in% +it$ radiation#

@301 Basic Radiolo%ical @ealt$

@302 Basic Radiolo%ical @ealt$ Refres$er

Before +or>in% +it$ $&man blood or tiss&es#

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b' T$e si%n m&st $ave information re%ardin% biosafety level*materials &sed* emer%ency contact name and p$one n&mber'

)iological .aste isposal

)ll recombinant material is considered infectio&s and m&st be disposed as biolo%ical+aste'

,nfectio&s +aste materials s$o&ld be c$emically disinfected +it$ 10H bleac$ or preferably* decontaminated in a steam a&toclave' ,nfectio&s +aste s$o&ld be placed in a bio$a ard a&toclave ba%'

Tie t$e ba% closed or &se a r&bber band* place in an a&toclave pan* and a&toclave for atleast 30 min&tes at 121K &nder 18 psi' Si%n t$e a&toclave record lo% and after cyclecompletion* place 4@S +aste treatment stic>er on t$e ba%* and place in a blac> tras$ ba%

before disposin% in t$e re%&lar tras$'

ertain a&toclaves need to be tested +it$ an a&toclave test pac>' 6or 9&estions* contact4@S' 6or problems +it$ t$e a&toclave* call 6acilities Services'

)SL 0 Safety Procedures

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T$is lab is rated as Biolo%ical Safety Level 1' BSL1 is s&itable for +or> involvin% +ell-c$aracteri ed a%ents not >no+n to consistently ca&se diseases in imm&nocompetent ad&lt$&mans* and present minimal potential $a ard to laboratory personnel and t$eenvironment'

"( Good Practices

1' T$e laboratory P,/s&pervisor controls access to t$e laboratory'

2' Lab personnel +as$ t$eir $ands after +or>in% +it$ potentially $a ardo&smaterials and before leavin% t$e laboratory'

3' 4atin%* drin>in%* smo>in%* smo>eless tobacco* $andlin% contact lenses*applyin% cosmetics* and storin% food for $&man cons&mption is not

permitted in laboratory areas' ,f food is stored* it is done so o&tside t$elaboratory area in cabinets or refri%erators desi%nated and &sed for t$is

p&rpose'

5' ?o&t$ pipettin% is pro$ibited and only mec$anical pipettin% devices are

&sed'

8' )ll proced&res are performed to minimi e t$e creation of splas$es and/oraerosols'

)( S&arps

:' Policies for t$e safe $andlin% of s$arps* s&c$ as needles* scalpels* pipettes*and bro>en %lass+are $ave been developed and implemented' $enever

practical* laboratory s&pervisors $ave adopted improved en%ineerin% and+or> practice controls t$at red&ce ris> of s$arps inD&ries'

' aref&l mana%ement of needles and ot$er s$arps are treated +it$ primaryimportance' "eedles are not bent* s$eared* removed from disposablesyrin%es or ot$er+ise manip&lated by $and before disposal'


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13' ?aterials to be decontaminated o&tside of t$e immediate laboratory are placedin a d&rable* lea> proof container and sec&red for transport'

15' ?aterials to be removed from t$e facility for decontamination are pac>ed inaccordance +it$ applicable local* state* and federal re%&lations'

( "dministrative

18' ) si%n incorporatin% t$e &niversal bio$a ard symbol is posted at t$e entranceto t$e laboratory +$en infectio&s a%ents are present' T$e si%n incl&des t$ename of t$e a%ent(s! in &se* and t$e name and p$one n&mber of t$elaboratory s&pervisor or ot$er responsible personnel' )%ent information is

posted in accordance +it$ t$e instit&tional policy'

1:' )n effective inte%rated pest mana%ement pro%ram is in place'

1 ' T$e laboratory s&pervisor $as doc&mented t$at laboratory personnel receive

appropriate trainin% re%ardin% t$eir d&ties* t$e necessary preca&tions to prevent e7pos&res* and e7pos&re eval&ation proced&res' Personnel receiveann&al &pdates or additional trainin% +$en proced&ral or policy c$an%esocc&r'

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Environmental Healt& and Safety#$ttp#//+++'&te7as'ed&/safety/e$s/inde7'p$p

Researc& Support and Compliance#$ttp#//+++'&te7as'ed&/researc$/rsc/

"ppendi% " 3 "rt&ropod Containment Level Guidelines

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http://www.utexas.edu/safety/ehs/index.phphttp://www.utexas.edu/research/rsc/http://www.utexas.edu/safety/ehs/index.phphttp://www.utexas.edu/research/rsc/


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for glassware whenever possible#,otification and 'ignage# *ersons entering the area are aware of the presence of arthropod vectors#B. Special Practices0AC6C and 0 C Approval# 0AC6C approval is re.uired for use of vertebrate animalsused as hosts# 0 C approval is re.uired for non-e"empt recombinant +,A protocols#4ousing of ,on-Arthropod Animals# Animals not necessary for culture of the arthropodsare not accessible to the arthropods# Animals used as hosts or blood sources may behoused within the insectary but are ade.uately protected from access by escapedarthropods# *rotocols for vertebrate animal use are approved by the local 0AC6C#Containment +uring lood-%eeding# Arthropods fed on host animals are prevented fromaccidental transfer to host cages# 7hen handling removing animals after e"posure toarthropods precautions must be taken to prevent arthropod escape through screenscovers and by flying# 4ost animals are inspected closely (e#g# concealment in fur earscrevices) and the primary container is sufficiently robust to prevent escape duringfeeding#!8

lood 'ource# $he blood source is considered as a source of inadvertent arthropodinfection and transmission# /easures are implemented to prevent such an event# 6seof sterile blood or blood from sources known to be pathogen-free is recommended# 0ncontrast use of blood from animals or humans whose disease status is uncertain is tobe avoided#

scaped Arthropod 4andling# scaped arthropods are killed or collected and properlydisposed of#

Accidental 5elease 5eporting# $he insectary director is notified promptly of accidentalrelease of vectors#C. Safety Equipment (Primary Barriers)

loves# loves are worn when handling host animals or blood used to feed thearthropods#$orso Apparel# 7hite laboratory coats gowns and or uniforms are worn at all times inthe insectary when handling blood and vertebrate animals#

Arthropod-'pecific *ersonal *rotective .uipment# *ersonal protective e.uipment isworn as appropriate e#g# respirators for arthropod-associated allergies particle maskshead covers#D. Facilities (Secondary Barriers)Location of 0nsectary# $he insectary area is separated from areas that are used for general traffic within the building#0nsectary +oors# +oors openings whether covered by rigid panels glass screensplastic sheets or cloth minimi&e escape and entrance of arthropods#0nsectary 7indows# 7indows if present effectively prevent escape of the smallestarthropods contained within#

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