bsl-3 laboratory facility and management - wdcm dr. yiping zhu ( bsl-3 laboratory... · bsl-3...
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Training Course of Microbial Resources Information Management and Utilization for Developing Countries
BSL-3 Laboratory
Facility and Management
Dr. Yiping ZhuSep. 14 2016
Pathogens and BSL-3 laboratory
There are about 10 million microbe species have
been identified in the world.
Among those, only about 400 microbe species are
pathogenic to humans, and a little more than 100
species are high pathogenic, but they can ause very
serious infecious diseases.
Nowadays, merging and reemerging infecious
diseases (SARS, Avian flu, Ebola, Zaka…) are big
threat to human socity.
We need to learn how to deal with, and how to control
the diseases.
A BSL-3 or BSL-4 laboratory is required when we
handling high pathogenic microorganisms.
What does a BSL-3 Lab look like?
When you go into a BSL-3 Laboratory…
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2
3
1
4
5
2
3
4
1 Biosafety Cabinet
Personal Protection Equipment
Biohazard Waste Container
Biohazard Warning Signs
Access control
Directed Air Flow
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The Sandwich structure of BSL-3 Lab
BSL-3 Lab Design Requirements:
a. 2 door entry (interlock)
b. Solid easily cleanable surfaces
(floor, walls, ceiling)
c. Coved floors
d. HEPA (high efficiency
particulate air filter) filtered air
supply and exhaust
e. Negative pressure and
directed air flow
f. Sealed penetrations
g. Hands-free sink near exit door
h. Eyewasher and Shower
i. Pass through autoclave
j. Sewage reatment system
Why build a BSL-3 Laboratory?
BSL-3 Laboratories are designated for handling high pathogenic
microbes that may cause serious or potentially lethal disease
through the inhalation route of exposure.
P3 and 3 Ps:
Physical Containment level 3 Lab
Protect people
Protect samples
Protect environment
What build up a BSL-3 Laboratory?
BiosafetyManagement
BSL-3 Lab
Facility & Equipments
EnsureBiosafety
Before start to build a BSL-3 Lab
Seismic safety assessment
2
When design and build a BSL-3 Lab
GB19489-2008:
Laboratories-General requirements
for biosafety
GB50346-2011:
Architectural and technical code
for biosafety laboratories
Commissioning and pass the Third Party Inspection
Capability accreditation by CNAS
China National Accreditation Service for Conformity Assessment
Laboratory qualification approval by
the Ministry of Health
The approval of handling high pathogenic microorganisms by
Beijing Municipal Health Bureau
Before start runing a BSL-3 Lab
Access control
When runing a BSL-3 Lab
Camera
Local police
Biosecurity
Protects hazards
from dangerous
people
Biosafety
Management
Biosafety
Protects people
from dangerous
hazards
Key points of Biosafety Management
Waste
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2
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6
3
4
Biosafety
Management system
Facility
Routine work
Operation
Staff
Material
Risk awearness
Safe operition skill
Emergency response Quiz
Operition test
Ability assessment
Regular health check
Immunization
Serum storage
Counseling
Medical services and first aid
1. Staff management
Training
Healthsurveillance
Evaluation
Heinrich's Law: that in a workplace, for every accident that causes a
major injury, there are 29 accidents that cause minor injuries and 300
accidents that cause no injuries.
Why staff training is primary efficient?
Classifications Level of Hazard
Risk Group 1 No or low individual and community risk.
Risk Group 2 Moderate individual risk, low community risk.
Risk Group 3 High individual risk, low community risk.
Risk Group 4 High individual and community risk.
2. Biohazard materials management
Pathogens Handling of biohazard materials
NameRisk
groupCulture
Animal
trials
clinical
sample
Inactived
samples
Non-
infectious
samples
Mouse leukemia
virus1 BSL-1 ABSL-1 BSL-1 BSL-1 BSL-1
Hepatitis B virus 2 BSL-2 ABSL-2 BSL-2 BSL-1 BSL-1
High pathogenic
avian influenza
virus
3 BSL-3 ABSL-3 BSL-2 BSL-1 BSL-1
Ebola virus 4 BSL-4 ABSL-4 BSL-3 BSL-2 BSL-1
Handling of biohazard materials
.
SOPs
Good
practice
.
3. Operation management
Risk
assessment
and control
Emergency
response
Supplies
Clean
Decontamination
emergency response
Incident report
4. Routine work management
5. Facility equipment management
a. Daily inspection
b. Regular maintainence
c. Annual check and test
d. Annual maintinence
a. contaminated sharps
b. solid wastes
c. cultures, stocks
d. liquid wastes
e. animal carcasses
6. Waste management
a. autoclave
b. chemical
c. incineration
d. monitoring (autoclave tapes,
biological indicators)
.
.
.
.
Waste decontamination
Waste segregation
Support, Service, Serious
Ensure the Biosafety of Your Research
Yiping Zhu13466626489, [email protected]