bs-200 kinetics

8/7/2019 BS-200 Kinetics

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BSBS--200 Parameter Setting200 Parameter Setting

(Kinetics)(Kinetics)

WendyWendyApr, 2008Apr, 2008


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PrefacePreface

Here we set ALT as the example forall Kinetics items on

Generally, Kinetics item includes: ALT, AST, ALP,ACP, CK, CK-MB, AMY, LIP, GGT, LDH,


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Input the item name forTest.

ALT


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No. will be generated automatically by the system, eg 1 for the first one.

1

ALT


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Full Name of the item, can be blank if the user dont want to input anything.

1

ALT


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StandardNo. of the item, can be blank or involved with the bar code.

1

ALT


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Reac.Type of the item, can be referred from the reagent insert as ...

1

ALT


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So we can set it as Kinetics type.

Indicates the absorbance should be monitored in exact minutes.

Basic parameter for ALTBasic parameter for ALT


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1

ALT

Reac.Type is set as Kinetics as the reagent insert indicates.

1

Kinetics


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1

ALT

1

Kinetics

Pri.Wave & Sec.Wave should be selected as reagent insert indicates


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So we can set it as 340 nm for Pri. Wave; since nosecondary wavelength is mentioned, Sec. Wave could be

blank.



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1

ALT

1

Kinetics

Pri.Wave is selected as 340 nm, Sec.Wave is blank.

340


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1

ALT

1

Kinetics

340

Direction of the absorbance changing during the reaction, refer to ...


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From Reaction

Principle, we can

see NADH(routineindicator under

340nm) is a to-be-consumed

reactant, which

will be less and

less during testing,

thus, indicatingthe absorbance is

lower and lower in

the measurement.

Thus, we can set

it as Descending



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1

ALT

1

Kinetics

340

Direction is selected as Descending.

Descending


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Mono reagent items' reaction time begins when Sample is dispensed.

Dual reagent items reaction time begins when R2 is dispensed.

The time between S and R2 dispensing is called Incubation time,

only dual reagent measurements have incubation time.

R1 Sample

Preheating time Reaction time

To be set

from the

insert

Fixed 14

cycles


Incubation

time

0 cycle of

reaction time

R2


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Time is all calculated into cycles on BS-200, and 1 cycleequals to 16 seconds.

Roughly speaking, we change minutes into cycles forReac. Time and Incuba. Time as below.

minute cycle

0.5 2

1 4

2 8

3 12

4 16

5 20



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1

ALT

1

Kinetics

340

Reac.Time is set as 4 and 16, and Incuba.Time as 20.

4 16

20

Descending


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1

ALT

1

Kinetics

340

Descending

Unit of the item, should be set according to the reagent insert

4 16

20


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Kinetics method is always testing enzymes, and usuallythe Unit is U/L.

According to the part of Reference Values, we can alsofind its proper to set the Unit as U/L.


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1

ALT

1

Kinetics

340

Descending

4 16

20

We can set the Unit as U/L.

U/L


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Its normal to report ALT results without decimals, so wecan set the Precision as 0.


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

Precisionof the item is set as 0.

0


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

R1, R2and Sample Volumeof the item, should be referred to

0


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To process with the sample testing at 37, we need 100ul sample,

1000ul R1 and 250ul R2 (namely, the ratio of S/R1/R2 is 2/20/5);

According to the volume range of BS-200 (sample: 3-45ul, R1: 180-

450ul; R2: 30-450ul), and the indicated S/R1/R2 ratio;

We can set Samplevolume as 18ul, R1 as 180ul, and R2as 45ul.



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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L0

R1, R2 and Sample Volumeare set as 180, 45 and 18.

18

180

45


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L0

Absorbance ofR1 Blank(mono rgt.) and MixedRgt. Blank(dual rgt.),

set according to experience or reagent insert; if no idea, can be blank.

18

180

45


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From the part of Linearity, we can get aware of that, themaximum absorbance change is 0.160 A/min, and theupper limit of Linearity Range is 350 U/L.


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

0

18

180

45

We can set the upper limit ofLinearityRangeas 350 U/L.

350


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

0

18

180

45

LinearityLimit should be set for Kinetics tests, refer to the instruction

350


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Background ofLinearity Limit check

Its always testing enzymes with the method of Kinetics;

In this kind of measurement, its supposed that theabsorbance changing speed remains constant during thereaction;

So it should be a straight line as the reaction curve; Linearity is to check if the reaction curve is straight

enough (thus, if the reaction is correct) to measure theconcentration of Kinetics items.

Value ofLinearity Limit setting

The linearity ranges from 0 to 1, and the default value is0.20000.

If set, BS-200 will automatically calculate thelinearity during every measurement (as the next slide )and compare the result to the linearity limit and flag the

result exceeding the defined limit.



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A1 A6. . . . At-5 . . . .At

Linearity = 100% (A6 A1) (At At-5)

At A1


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

0

18

180

45

According to the instruction, LinearityLimit can be set as 0.200000.

350

0.200000


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

0

18

180

45

SubstrateLimitshould be set for Kinetics tests, refer to the instruction

350

0.200000


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Background ofSubstrate Limit check

Its always testing enzymes with the method of Kinetics;

In this kind of measurement, its supposed that there issurplus substrate in the reagent (enough for the testing);

When the samples concentration is higher than its linearrange, the substrate will be exhausted, and then thetesting result will not be correct;

Substrate Limit is to check if the reaction is processedcorrectly.

Value ofSubstrate Limit setting

The Substrate Limit can be set from 0 to 50000(absorbance);

In ascending reactions, it could be 20000~25000; and itcould be 0~5000 for descending reactions (eg nextslide ).

If set, BS-200 will automatically compare absorbancevalue with the Linearity Limit and flag the result exceedingthe defined limit.



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Since its a descending reaction, we can set 5000 for ALT; itmeans when the absorbance becomes lower than 5000, BS-200

will flag the result reminding you to rerun this sample.

5000


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

0

18

180

45

350

0.200000

According to the instruction, SubstrateLimit can be set as 5000.

5000


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

0

18

180

45

Once Factoris set, no calibration is required; it should be referred from

350

0.200000

5000


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According to Calculation in the reagent insert and thereaction temperature of 37, we can set 2143 for theFactor.



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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

0

18

180

45

Factoris set as 2143. However, we recommend to calibrate ourselves.

350

2143

0.200000

5000


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1

ALT

1

Kinetics

340

Descending

4 16

20

U/L

0

18

180

45

350

2143

Prozonecheck is set for the turbidimetric tests, which can also be blank.

0.200000

5000


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The basic parameters for ALT havebeen finished

Next, we should set the reagentinformation as following

Reagent parameter for ALTReagent parameter for ALT


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Basic reagent information (Position andBottle Type) has been set up

We should set relevant calibration and QC

information for the item

(Please refer to the video of Calibration & QC setting)

Calibration and QC for ALTCalibration and QC for ALT


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Thank you!Thank you!NOTE:Kinetics items should be set in the same way as ALT.

bs-200 kinetics

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