New Biotechnology · Volume 31S · July 2014 BIOFUELS, BIOCHEMICALS AND BIOENERGY

Biofuels, biochemicals and bioenergy

PB-01

Minimization of Bacterial Contamination with HighSolid Loading during Ethanol Production from Lignocel-lulosic Materials

Mofoluwake Ishola1,∗ , Tomas Brandberg2, MohammadTaherzadeh2

1 Swedish Centre for Resource Recovery, University of Borås2 University of Borås

Abstract Ethanol is the most important renewable fuel in thetransportation sector. Its production from lignocellulosic mate-rials, commonly referred to as second generation ethanol, isconsidered more attractive than production from starch and sugarcrops. Bacterial contamination by lactic acid-producing bacteria isstill a major problem during ethanol production processes. Bacteriacompete with the yeast by consuming the sugars and the nutrientsrequired by the yeast for efficient ethanol production. This oftencauses substantial economic losses at industrial fermentations.In this study, without any sterilization of the substrate, simul-taneous saccharification and fermentation (SSF) was performedusing cellulase Cellic® Ctec2 enzyme for hydrolysis and Baker’syeast, Saccharomyces cerevisiae, was used as the fermenting organ-ism with different loads of suspended solids - 8%, 10% and 12%.With 8% and 10% SS, there was a significant contamination, whichcaused consumption of both hexoses pentose sugars in the fer-mentation medium, this resulted in lactic acid concentrations of43 g/L and 36 g/L from 10% SS and 8% SS respectively. In con-trast, only 2.9 g/L lactic acid was observed with 12% SS. An ethanolconcentration of 47 g/L was produced from high solid loading of12% SS while just 26 g/L and 23 g/L were produced from 10% and8% SS respectively. Our results show that SSF with 12% SS hasan increased concentration of inhibitors, particularly acetic acidwhich selectively inhibited the bacterial growth without affectingthe metabolic activities of the yeast during the fermentation

Keywords Bacterial contamination; Lignocellulosic ethanol;Saccharomyces cerevisiae, lactic acid.

http://dx.doi.org/10.1016/j.nbt.2014.05.1828

PB-02

Breeding low temperature resistant Camelina sativa forbiofuel production

Florentina Matei1,∗ , Florentina Sauca2, Paul Dobre3, StefanaJurcoane2

1 University of Agronomical Sciences and Veterinary Medicine Bucharest, Fac-ulty of Biotechnologies2 Centre of Microbial Biotechnology Biotehgen, Bucharest, Romania3 University of Agronomical Sciences and Veterinary Medicine Bucharest, Fac-ulty of Agriculture, Bucharest, Romania

In the European effort to produce sustainable aviation fuel ourteam is involved in a FP7 project (ITAKA) in breeding activitiesof Camelina sativa L. as main feedstock. One of our purposes it

is to obtain a variety with high productivity and resistant to lowtemperatures specific to continental winters as can occur in Roma-nia. This variety may be seeded as autumn culture under minimaltilling conditions.

As starting material have been used a local camelina cultivarresistant to low temperatures and an international variety GP202with high productivity and oil content. It has been taken intoaccount classical approach, the use of immature embryo rescuetechnique. The first hybrid generation was obtained by castra-tion and pollination; the immature embryos were cultivated inMS medium w/o hormones; new plantlets were cultivated till fullmaturity under greenhouses conditions. The obtained seeds havebeen used in open field for other randomized hybridization.

The new hybrid registered the following characteristics: theplant lengths and the branching level are non-significantly dif-ferent compared to the parental lines (ANOVA tests); in terms ofproductivity, GP202 variety kept its top position, while the newhybrid has proven top position for oil content (2.13% higher intotal fat). Regarding the winter resistance, the hybrid has registered10% more survived plants in open land. As a remark, during theexperiments GP202 showed the highest sensibility to the mildewattack. The further aim of the work is to fix the new characters inorder to homologate the new variety.

http://dx.doi.org/10.1016/j.nbt.2014.05.1829

PB-03

Advanced Biomass Value: Microalgae biomass as a newsource of sustainable aviation biofuels and lubricantproduction

Felix Bracharz ∗ , Jan Lorenzen, Farah Qoura

TUM, Industrial Biocatalysis

The aviation industry grows 5% p.a. and by 2020 has to complywith strict governmental emission standards that mandate a 20%CO2 reduction compared to the emission levels measured at 2005.Together with the eminent end of fossil resources these driversforce the development of sustainable aviation fuel alternativesthat are carbon neutral and in compliance fuel standard regula-tions such as Jet A. In this study a new, mass-and energy efficientalgae biorefinery concept for the integrated production of aviationfuels, industrial lubricants and CO2 adsorbing building materialshas been developed. The process chain is based on production offast growing microalgae biomass containing up to 20%w/w lipids.Subsequently, algae lipids are separated from the biomass frac-tion and converted to high performance, high value lubricantsby targeted functionalisation using a cascade of optimized biocat-alytic processes. The biomass residue is enzymatically hydrolysedand used as a fermentation substrate for oleaginous yeast strains.Since these fast growing, oleaginous yeasts can accumulate up to80% w/w lipids, they are the ideal biomass base for the produc-tion of drop-in aviation fuels. Conversion of wet yeast biomassis accomplished using a streamlined thermochemical process thatfeatures optimized heterogenous catalysts. In this process carbonrich coke is a residue of the thermochemical biomass processing.This residue stream is used as a settling modifier in the production

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