“Breeding, agronomic and biotechnological approaches for reintegration and revalorization of

legumes in Mediterranean agriculture“

Diego RUBIALES CSIC, Spain

ARIMNet projects mid-term event June 11, 2014, Montpellier, France

2012-2015

Legumes are multifunctional crops with extraordinary importance for the agriculture, environment and culture

in Mediterranean countries

However acreage is continuosly decreasing

and we are importing legumes for food and feed

2012-2015

Cooperative multidisciplinary initiative to promote legume revalorization in Mediterranean agriculture

The main objective of this project is to develop methodologies to improve grain legume plant material of high yield potential, resistant to major diseases and

abiotic stresses, suitable for sustainable farming systems.

The selection of genotypes carrying the desired genes will be greatly facilitated by using molecular markers tightly linked to the desired traits. In this sense, pea will be used as a model as the best characterized crop legume.

(I) Definition of ideal genotypes, (II) Studies on biotic constraints (III) Studies on abiotic stresses (IV) Marker Assisted Selection (MAS) (V) Epidemiology (VI) Exploitation of alellopathy for disease control.

MEDILEG Project

“Breeding, agronomic and biotechnological approaches for reintegration and revalorization of legumes in Mediterranean agriculture“

Interactions with other consortia

FP7 2013-2016

FP5

FP6

FP6: LEGRESIST

FP7 2014-2017

Relevant dates: ARIMNet-Medileg - July 2011: expression of interest

- September 2011: submission deadline - March 2012: communication on project approval and new funding conditions

-From April 2012: Negotiations with national funding agencies

- August 2012: official start date

- Kick-off meeting: Tunis 4-6 September 2012

MEDILEG- ARIMNet

- September 2012: Kick off meeting in Tunis.

- 01 August 2012 agreed as project starting date

- September 2013: mid-term project meeting in Rennes

- Consortium agreement signed by all institutions

- Seeds provided for WP1, Seed Transfer Agreement prepared by INRAT

- WPs revised

- Month 6 report submitted to ARIMNET

- MEDILEG website created

WP0: Research coordination of the consortium Financial management will be handled directly between national researchers and

their national funding organizations in each participating country. T0.1: Facilitate close interaction between the Partners being the single point of contact between the ARIMNet Call Office and the Researchers T0.2: Draw a Consortium Agreement in order to manage the delivery of the project activities, finances, Intellectual Property Rights (IPR) and to avoid disputes which might be detrimental to the completion of the project T0.3: Redirect specific parts of the project if required by the progress of the Work packages T0.4: Compile and submit reports/deliverables to the ARIMNet Office on behalf of the Research Consortium (M6, M18, M36) T0.5: Meeting calendar: kick-off, mid-term and final meetings

WP1: Identification of regional priorities and definition of target genotypes

Coordinator: Dr Mohamed KHARRAT, INRAT Tunis D1.1: Definition of phenotypes and genotypes adapted to sustainable agriculture demands in different regions D1.2: Information and protocols for field evaluation of agronomic traits and resistance to biotic and abiotic constrains D1.3: Selection of the most representative lines/varieties that might serve as source gene-pool for genotype enhancement

WP2: Studies relevant for breeding for resistance to major biotic constraints

Coordinator: Prof. Diego RUBIALES, CSIC, Spain D2.1: Rapid and reliable screening methods suitable for selection and breeding D2.2: Development of breeding material with improved resistance tested at a wide range of environments and pathogen populations D2.3: Catalogue of sources of resistance

Agreement at Kick-off metting:

Participation in WP2 to be agreed disease by disease: year 1 screening of local germplasm. Most resistant accessions to be compiled in a joint disease resistance nursery to be validated in different

countries using common protocols

WP3: Studies relevant for breeding for major abiotic constraints Coordinator: Dr Marion PRUDENT, INRA-LEG, France D3.1: Standardized experimental conditions for drought and salt tolerance D3.2: Rapid methods for screening for drought and salinity tolerance D3.3: Identification of sources of resistance to drought and salinity (on various species, and in combination with particular Rhizobium strains)

WP4: Marker Assisted Breeding Coordinator: Dr. Alain BARANGER, INRA-IGEPP, France D4.1: List of polymorphic eSSR and SNP markers for genetic mapping and pea map comparisons D4.2: A set of SNP markers in pea designed in genes involved in stress resistance and plant architecture and development D4.3: High density pea linkage maps from populations segregating for resistance biotic and abiotic factors D4.4: Identification, and determination of the genetic effects of the QTLs controlling polygenic traits D4.5: Information on QTL stability across diverse field locations, pathogen variability and plant genetic backgronds D4.6: Development of standard markers for pyramiding and rapid screening D4.7: List of candidate genes likely to be involved in resistances to stresses.

WP5: Epidemiology Coordinator: Dr. Christophe LE MAY, INRA-IGEPP, France D5.1: Clarification of the genetic variation and adaptive potential of ascochyta species D5.2: Identification of new control levers to better control disease development. D5.3: Determine control method combinations that will decrease disease development. D5.4: Identification of architectural features that will limit the spatiotemporal dispersal of the disease. D5.5: Development of standard differential sets for race identification.

WP6: Biological control and allelopathy studies Coordinator: Prof. Antonio EVIDENTE, UNINA, Italy D6.1: Protocols for the extraction and purification of bioactive metabolites (phytotoxins, herbicides, phytoalexins) D6.2: Structure determination of new bioactive compounds with original carbon skeleton D6.3: Characterization of phytotoxic, antifungal, mycotoxic and allelopatic activities of the pure metabolites isolated D6.4: Preparation of key derivatives for structure confirmation and structure-activity relationships studies

WP7: Dissemination Coordinato: Dr. Lamiae GHAOUTI, IAV, Morocco D7.1: Methodology and biotechnological protocols transference to facilitate the selection of interesting genotypes D7.2: Scientific papers, divulgation articles in farmer journals and contributions to conferences D7.3: Project web site

T0.2: Draw a Consortium Agreement in order to manage the delivery of the project activities, finances, Intellectual Property Rights (IPR) and

to avoid disputes which might be detrimental to the completion of the project

- 1st draft circulated 15th June 2012 - Signed by all partners Contact person:

Francisco OROZCO Consejo Superior de Investigaciones Científicas Legal Unit for European Programmes Serrano, 113 – 28006, Madrid Francisco.orozco@orgc.csic.es Tel: 0034 91 568 1915

Reports:

•National reports at national level to national authorities No additional funds to be expected from EU: our only budget is the one provided to each country

•ARIMNET reports (M6, M18, M36): partners report to WP coordinators who prepare WP reports to be compiled by project coordinator and submitted to ARIMNET office

•Deliverable reports**

“digested results” to be provided by partners to WP coordinators

Who and how gathers and analyzes the data and prepared possible publications is to be discussed case by case

T0.4: Compile and submit reports/deliverables to the ARIMNet Office on behalf of the Research Consortium (M6, M18, M36)

1. Identification of project and participants 2. Publishable summary Short description of activities, work progress This description should be written for a non-technical audience (max 1 page) 3. Deviations from proposal/work plan Explain any deviations from proposal/work plan and impact on other tasks. Describe corrective actions adopted or proposed for deviations from tasks. Please also use this section to summarize any changes you propose to your project, compared to the original proposal/work plan.

Periodic report (M6)

template – periodic report (M18) 1. Identification of project and participants 2. Publishable summary 3. Work progress and achievements during the period (max 3 pages) 4. Deliverables 5. Deviations from proposal/work plan 6. Dissemination activities in the period in question (including list of publications) 7. Project management (including list of meetings) 8. Financial report (period M1 to M12 –included)

Periodic report (M18)

T0.5: Meeting calendar: - Kick-off meeting: Tunis 4-6 September 2012 - Mid-term meeting: Rennes 5-6 September 2013 - Final meeting: Rabat, May 2015

T7.3: MEDILEG project website for dissemination It will be used as the repository of all information concerning the project and actively used

to monitor project goals and targets for all work packages. It will be a source of information for all stakeholders with publications that are focused on meeting their specific

needs and interests.

www.ias.csic.es/medileg

www.ias.csic.es/medileg

Interactions with other consortia

FP5

Interactions with other consortia

FP6

Interactions with other consortia

FP6: LEGRESIST

Interactions with other consortia

FP7 2013-2016

Interactions with other consortia

FP7 2014-2017