† This statement has not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.

CARDIOVASCULAR HEALTH

† This statement has not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.

BONITO PEPTIDE

• Promotes healthy blood pressure levels• Supports Cardiovascular Health• Promotes Cerebrovascular Bloodflow

Bonito, also called katsuobushi, is a small fish commonly consumed in Asian countries. The active peptides, or isolated prepared proteins, of the bonito fish are known to support healthy blood pressure, mediated by their ability to inhibit angiotensin l-converting enzyme (ACE)activity,[ 1] which makes them an integral component of cardiovascular support protocols. Ortho Molecular’s bonito peptides product is standardized to contain3.4 mg/gram of the isolated peptide LKPNM, which is converted by ACE to LKP, a peptide that provides eight times the ACE-supporting activity of the parent bonito compound. Studies have shown that 1.5 g per day of bonito peptides is beneficial for maintaining optimal blood pressure levels.

OverviewJapanese researchers have identified a patented process to isolate specific, active peptides from the bonito fish (Sarda orientalis), which has been shown to help maintain healthy blood pressure. Bonito peptides have been shown to inhibit ACE activity, the enzyme responsible for the conversion of angiotensin I to angiotensin II. [3] By inhibiting the formation of this compound, bonito peptides relax the arterial walls and reduce fluid volume supporting healthy blood pressure. The main active bonito peptide (called LKPNM, based on its amino acid sequence) is converted by ACE to LKP, the active peptide shown to significantly support healthy blood pressure levels.

CLINICAL APPLICATIONS

Research†An 8-week study of 30 patients found that ingestion of1.5 g/day bonito peptides, containing 5 mg of LKPNM, was shown to modulate ACE activity, and helped maintain systolic and diastolic pressure balance.[2] A crossover study of 37 patients examining the effects of a bonito peptide soup containing 1.5 g of bonito peptides and 5 mg LKPNM, found the bonito broth significantly modulated ACEactivity.[3] Another study comparing the effects of bonito extract showed bonito more potently relaxed contractions induced by norepinephrine (a chemical involved with heightening arousal and inducing a stress response) and slightly inhibited contractions triggered by angiotensin I. This study highlighted bonito peptides’ direct action on vascular smooth muscle and ACE-inhibitory activities.[4] Bonito extract has also been found to help maintain cerebral blood flow and relaxation of the blood vessels through the production of nitrogen oxide and the suppression of active oxygen regeneration. [5]

Directions1 capsule three times per day or as recommended by your health care professional.

Does Not ContainGluten, corn, yeast, artificial colors and flavors.

CautionsIf you are pregnant or nursing, consult your physician before taking this product.

CARDIOVASCULAR HEALTH

† This statement has not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.

References1. Fujita H, Yoshikawa M. LKPNM: a prodrug-type

ACE-inhibitory peptide derived from fish protein. Immunopharmacology Oct 15 1999;44(1-2):123-127.

2. Ohshima K. Human Volunteers Study on Antihypertensive Effect of “Katsuobushi Oligopeptide” (I). Jpn Pharmacol Ther 1997;25(8):147-151.

3. Ohshima K. Human Volunteers Study on Antihypertensive Effect of “Katsuobushi Oligopeptide” (II). A placebo-controlled study on the effect of “peptide soup” on blood pressure in borderline and hypertensive subjects. Jpn Pharmacol Ther 1997;25(8):153-157.

4. Kouno K, Hirano S, Kuboki H, Kasai M, Hatae K. Effects of dried bonito (katsuobushi) and captopril, an angiotensin I-converting enzyme inhibitor, on rat isolated aorta: a possible mecha¬nism of antihypertensive action. Biosci Biotechnol Biochem 2005; Vol 69. 911-915.

5. Honda M, Yamada H, Nozawa Y, Ishizaki T, Kuroda M,Noguchi T. Consumption of bonito extract suppresses the decrease in cerebral blood flow in stroke-prone spontaneously hyperten¬sive rats. Biomed Res 2010;31(4):251-258.

1 capsule contains Amount Per

Serving% Daily

Value

V1

Bonito Peptide

Serving Size 1 CapsuleServings per Container 90

* Daily Value not established

(Standardized to contain 85% peptides)500 mg *

Supplement Facts

ID# 589090 90 Capsules