1

BITA BAKHSHI

OBJECTIVE

Position: Associate Professor

Address: Department of Bacteriology, Faculty of Medical Sciences,

Tarbiat Modares University, Tehran, Iran.

E-Mail: b.bakhshi@modares.ac.ir

Tel: 021-82884558

EDUCATIONS

Post-Doctoral Fellowship 2009- 2010

R &D Department, Production complex, Pasteur Institute of Iran, Tehran, Iran.

Construction and Production of Recombinant Cholera Toxin B Subunit

(rCTB) in order to Manufacture WC-CTB Vaccine Against Cholera

Ph.D in Medical Bacteriology 2003- 2008

Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of

Medical Sciences, Isfahan, Iran

Thesis title: Investigation of the genotypig pattern of clinical and

environmental isolates of Vibrio cholerae serotype O1 and study of genetic

structure of CTX and VPI clusters of them

2

M.Sc. in Medical Microbiology 2000-2003

Department of Bacteriology, Faculty of Public Health, Tehran University of Medical

Sciences, Tehran, Iran

Thesis title:Comparison of culture and polymerase chain reaction in

detection of mycoplasma in the synovial fluid of patients with arthritis.

B.Sc. in Microbiology 1994-1998

Faculty of Sciences, AlzahraUniversity,Tehran, Iran

LANGUAGE SKILLS

1992-1996: Graduated from Level 12 of Iran Language Institute (ILI)

Senior Document in English Language from ILI

PARTICIPATION IN RESEARCH PROJECTS

1997-1998 Contribution to research project entitled: Zeolite Project and

Study of its Application in Industries, Mine and Metal Ministry, Tehran,

Iran.

2002-2003 Contribution to research project entitled: Molecular Analysis of

the Prostat Specific Antigen and Prostat Membrane Specific Antigen in the

Blood of Patients with Prostat Cancer Reffered to the Tehran University

of Medical Sciences Hospitals Using RT-PCR, Tehran University of

Medical Sciences, Iran.

2005-2006 Contribution to research project entitled: Molecular

epidemiology of Vibrio cholerae in Iran. Department of Bacteriology,

Pasteur Institute of Iran, Tehran, Iran

3

2006-2008 Contribution to research project entitled: Genotypic analysis of

the Environmental Isolates of Vibrio choleraeand Examination of Virulence

and Resistance Associated Genes, Pasteur Institute of Iran, Tehran, Iran.

2009-2010 Executive of the research project entitled: Construction and

Production of Recombinant Cholera Toxin B Subunit (rCTB) in order to

Manufacture WC-CTB Vaccine against Cholera.

2010-2012 Executive of the research project entitled: Comparison of the

Structure of O-Antigen Biosynthesis Genes among Vibrio cholerae O1

Ogawa and Inaba Serotypes Isolated from Patients in Iran During 2004-

2008 and IS1004 Profiling of the Strains Using Southern Blot Hybridization

Technique. Iran National Science Foundation.

2012-2013 Executive of the research project entitled: Investigation of

Immunological responses against recombinant cholera toxin B subunit

produced in Escherichia coli and Whole cell-CTB as a candidate of vaccine

against cholera disease in rabbit model, Iran National Science Foundation.

2012-2013 Executive of the research project entitled: Investigation of

distribution and diversity of resistance gene cassette of class 1 integrons

among Campylobacter spp. Isolated from foods and patients and investigation

of genetic diversity of isolates using Pulsed-Field Gel Electrophoresis.

Antimicrobial resistance research center, Tehran, Iran.

2011- continued- Executive of the research project entitled: Investigation

of genetic diversity of enteropathogenic bacteria. TarbiatModares

University, Tehran, Iran.

4

AWARDS/GRANTS

Winner of Travel Grant. based on the quality of abstract presented in

17th European Congress of Clinical Microbiology and Infectious Diseases,

Munich, Germany, 31 March- 3 April 2007.

Winner of award in10th Iranian Congress of Microbiology, Kerman, Iran,

based on the best oral presentation.

Winner of award in 6th Iranian Congress of Microbiology, Feb. 16st- Jan.

18rd, Tehran, Iran, Based on the best poster presentation.

Winner of Travel Grant. based on the quality of abstract in 19th

European Congress of Clinical Microbiology and Infectious Diseases, Helsinki,

Finland, 16-19 May, 2009.

A Grant for the research project on “genotyping of the Salmonella

enterica serotypes” from EMHGBN.

FULL ARTICLES PUBLISHED IN REFEREED JOURNALS

1. Bakhshi B.; Alizadeh N.; Badami N.; Khorramizadeh M.R.;

Kheradmand. Attempt to detect Mycoplasmas and Chlamydias by

Culture in the Synovial Fluid of Patients with Arthritis. Iran Scientific

Journal of Public Health, 2002; 31: 111-112. (IF: 0.2).

2. Pourshafie M.R.,Bakhshi B., Ranjbar R, Sadaghat M., Sadeghifard

N., ZaemiYazdi J., Parzadeh M., Raisi J. Dissemination of a single

Vibrio cholerae clone in cholera outbreaks during 2005 in Iran. Journal of

Medical Microbiology. 2007; 56: 1615-1619. (IF: 2.38).

5

3. Bakhshi B., M.R. Pourshafie, F. Navabakbar, A. Tavakoli. Genomic

organization of CTX element among toxigenic Vibrio choleraestrains.

Clinical Microbiology and Infection. 2008; 14: 562-568. (IF: 4.78).

4. Bakhshi B., M.R. Pourshafie, F. Navabakbar, A. Tavakoli.

Comparison of Distribution of Virulence Determinants in Clinical

and Environmental Isolates of Vibrio cholerae. Iranian Biomedical Journal.

2008; 12: 165-169.

5. Bakhshi B., MohammadiBarzelighi H., AdabiM., RastegarLariA.,

M.R. Pourshafie. A molecular survey on virulence associated

genotypes of non-O1 non-O139 Vibrio cholerae in aquatic

environment of Tehran, Iran. Water Rsearch.2009; 43: 1441-7. (IF: 4.5)

6. Bakhshi B., M.R. Pourshafie.Assessing clonality of Vibrio cholerae

strains isolated during 4 consecutive years (2004-2007) from Iran.

Scandinavian journal of infectious diseases. 2009; 4: 256-62. (IF: 1.7).

7. Adabi M., Bakhshi B.,Goudarzi H,Zahraei M.,Pourshafie M.R.

Distribution of Class I integron and SXT constin in Vibrio

choleraeisolates from patients in Iran. Microbial drug resistance. 2009;

15:179-84. (IF: 1.98).

8. Dashtbani A., Bakhshi B. Pourshafie M.R. Global comparison of

recA gene sequences from clinical and environmental Vibrio cholerae

strains isolated in Iran. Letters in Applied Microbiology, 2011, 53(3):313-

23. (IF: 1.6).

9. MohammadiBarzelighi H., Bakhshi B., RastegarLari A., M.R.

Pourshafie.Characterization of pathogenicity island prophage in

clinical and environmental strains of Vibrio cholera. Journal of Madical

Microbiology, 2011;60:1742-9. (IF:2.38).

6

10. MoazeniJoula GR, Bakhshi B, Tadayon K, Razazi F, Banihashem

MA. Antibiotic resistance/suseceptibility of 30 isolates of Bacillus

anthracis. Archives of Razi institute. 2011; 2: 115-20.

11. Dashtbani-Roozbehani A, Bakhshi B, Pourshafie MR, Katouli M.

A Rapid and Reliable Species-specific Identification of Clinical and

Environmental Isolates of Vibrio choleraeUsing a Three-test

Procedure and recA PCR. Indian Journal of Medical Microbiology . 2012;

30(1):39-43. (IF: 1.0).

12. Bakhshi B., Sharifnia A.,Mohammad Katouli M.R. Pourshafie.wbeT

sequence typing and IS1004 profiling of Vibrio cholera isolates in Iran.

Letters in Applied Microbiology (2012, accepted) (IF: 1.6).

13. Najibi S, Bakhshi B,Fallahzad S, Pourshafie MR, Katouli M, Sattari

M, Alebouyeh M, Tajbakhsh M. Distribution of class 1 integrons

among enteropathogenicEscherichia coli. Canadian Journal of

Microbiology. 2012; 58(5): 637-43. (IF:1.2).

14. Bakhshi B, MohammadiBarzelighiH,Sharifnia A, Dashtbani-

Roozbehani A, Rahbar M, Pourshafie MR. Presence of CTX gene

cluster in environmental non-O1/O139 Vibrio cholerae and its

potential clinical significance. Indian Journal of Medical Microbiology .

2012; 30(3): (IF: 1.0).

15. Hosseini Aliabad N, Bakhshi B, Pourshafie MR, Sharifnia A.

Ghorbani M. Molecular Diversity of CTX Prophage in Vibrio

cholerae. Letters in Applied Microbiology. 2012; 55(1): 27-32. (IF: 1.6).

16. Bakhshi B, Boustanshenas M, Mahmoudi-aznaveh A. Emergence

of Vibrio cholerae O1 classical biotype in 2012 in Iran. Letters In

Applied Microbiology. 2014; 58(2): 145-149.

7

17. Boustanshenas M, Bakhshi B, Ghorbani M, Norouzian D.

Comparison of two recombinant systems for expression of

cholera toxin B subunit from Vibrio cholerae. Indian Journal of

Medical Microbiology. 2013; 31(1): 4-10.

18. Boustanshenas M, Bakhshi B, Ghorbani M.Investigation into

immunological responses against a native recombinant CTB

whole-cell Vibrio cholerae vaccine in a rabbit model. Journal of

Applied Microbiology. 2013; 114(2): 509-15.

19. Boustanshenas M, Bakhshi B. Thehows and whys of

constructing a native recombinant cholera vaccine. Bioengineered.

2013; 5(1): 1-3.

20. Eftekhari N, Bakhshi B, Pourshafie MR, Zarbakhsh B, Rahbar

M, Hajia M, Ghazvini K Genetic diversity of Shigella spp. and

their integron content. Foodborne Pathogens and Diseases. 2013;

10(3): 237-42.

21. Dashtbani-Roozbehani A, Bakhshi B, Pourshafie MR Genetic

relatedness of clinical and environmental Vibrio cholerae isolates

based on triple housekeeping gene analysis. Current Microbiology.

2013; 67(1): 15-20.

22. Bakhshi B, Fallahzad S, Pourshafie MR. The occurrence of

atypical enteropathogenic Escherichia coli strains among children

with diarrhea in Iran.J Infect Chemother. 2013; 19(4): 615-20.

23. Ghazi M, Khanbabaee G, Fallah F, Kazemi B, Mahmoudi S,

Navidnia M, Pourakbari B, Bakhshi B, Goudarzi H.Emergence

of Pseudomonas aeruginosa cross-infection in children with cystic

fibrosis attending an Iranian referral pediatric center. Iran J

Microbiol. 2012; 4(3): 124-9.

8

24. Marashi SM, Bakhshi B, Fooladi AA, Tavakoli A, Sharifnia A,

Pourshafie MR. Quantitative expression of cholera toxin mRNA

in Vibrio cholerae isolates with different CTX cassette

arrangements. 2012; 61(Pt 8): 1071-3.

25. BitaBakhshi, HajarMohammadi-Barzelighi, NedaHosseini-

Aliabad,Mohammad R. Pourshafie. Ribotyping and TCP gene

cluster analysis of environmental and clinical Vibrio cholerae

strains isolated in Iran. Annals of Microbiology. 2013.

26. AzamMahmoudi-Aznaveh, BitaBakhshi, ShahinNajar-Peerayeh,

AnoshirvanKazemnejad, Zahra Rafieepour, Mohammad Rahbar,

ShahlaAbbaspour. Commensal E. coli as an Important Reservoir

of Resistance EncodingGenetic Elements. Int J Enteric

Pathogens. 2014; 1(2): 43-7.

27. MasoumehNavidinia, ShahinNajarPeerayeh, FatemehFallah,

BitaBakhshi. Phylogenetic Groups and Pathogenicity Island

Markers in Escherichia coli Isolated From Children. Jundishapur

J Microbiol. 2013; 6(10): 1-5.

28. SafouraDerakhshan, ShahinNajarPeerayeh, FatemehFallah ,

BitaBakhshi, Mohammad Rahbar, Abbas Ashrafi. Detection of

Class 1, 2, and 3 IntegronsAmongKlebsiellapneumoniae Isolated

from Children in Tehran Hospitals. Archives of Pediatric

Infectious Diseases. 2013; 1(4): 164-8.

29. Maryam Roudbary, ShahlaRoudbarmohammadi, BitaBakhshi,

Zahra Farhadi, FatemehNikoomanesh. Identification of Candida

species isolated from Iranian women with vaginal candidiasis by

PCR-RFLP method. European Journal Of Experimental Biology.

2013; 3(6): 365-369.

9

30. Maryam Roudbary, ShahlaRoudbarmohammadi, BitaBakhshi,

Zahra Farhadi. Relation of ALS 1 and ALS3 genes and

fluconazole resistance in Candida albicans isolated from vaginal

candidiasis. International Journal of Molecular and Clinical

Microbiology. 2012; 2: 170-174.

31. MasoumehNavidinia, ShahinNajarPeerayeh, FatemehFallah,

BitaBakhshi, SaadatAdabian, ShadiAlimehr, ZariGholinejad.

Distribution of the pathogenicity islands markers pals in

uropathogenic e coli isolated from children in mofid children

hospital. Archives of Pediatric Infectious Diseases. 2013;1(2):75-

79.

32. Mohammad R. Samaei, Seyed B. Mortazavi, BitaBakhshi,

Ahmad JonidiJafari. Identification, and degradation characteristics

of n-hexadecane degrading bacteria from tropical areas in Iran.

Fresenius Environmental Bulletin. 2013; 22(4b): 1304-12.

33. Bita Bakhshi, Nazanin Eftekhari, Mohammad R.

Pourshafie.Genetic elements associated with antimicrobial

resistance among intestinal bacteria. Jundishapur Journal of

Microbiology. 2014;

7(5): e9924.

34. BitaBakhshi, Mina Boustanshenas, MasoudGhorbani. A single

point mutation within the coding sequence of cholera toxin B

subunit will increase its expression. Iranian Biomedical Journal.

2014;18(3):130-5.

35. Mahdi Ghorbanalizadgan, Bita Bakhshi, Anoshirvan

Kazemnejad Lili, Shahin Najar-Peerayeh, Bahram Nikmanesh. A

Molecular Survey of Campylobacter jejuni and Campylobacter Coli

10

Virulence and Diversity. Iranian Biomedical Journal. 2014;

18(3):158-64.

36. BitaBakhshi, SakineNajibi, Saeed Sepehriseresht. Molecular

characterization of Enterohamorrhagic Escherichia coli isolates

from cattle. Journal of Veterinary Medical Sciences. 2014 76(9):

1195–1199.

37. Pourabdollah M, Torkian A, Hashemian SJ, Bakhshi B. A triple

fouling layers perspective on evaluation of membrane fouling

under different scenarios of membrane bioreactor operation.J

Environ Health Sci Eng. 2014 Jun 6;12:91.

38. Abdolmajid Ghasemian, ShahinNajarPeerayeh, BitaBakhshi,

Mohsen Mirzaee. Inducible Clindamycin Resistance in Clinical

Isolates of Staphylococcusaureus. Arch Clin Infect Dis. 2014 April;

9(2): e16218.

39. Abdolmajid Ghasemian, ShahinNajarPeerayeh, BitaBakhshi,

Mohsen Mirzaee.Detection of accessory gene regulator groups

genes and cassettechromosome mectypes among Staphylococcus

aureusisolated fromintensive care unit patients.Asian Pac J Trop

Dis 2015; 5(2): 153-157.

40. Bita Bakhshi.The role of filamentous CTXphi bacteriophage in

Vibrio cholerae genetic and diversity. Reviews in Medical

Microbiology. 2014; 25.

41. Masoumeh Navidinia, Shahin Najar Peerayeh, Fatemeh Fallah,

Bita Bakhshi, Raheleh Sadat Sajadinia. Phylogenetic grouping

and pathotypic comparison of urine and fecal Escherichia coli

isolates from children with urinary tract infection. Brazilian

Journal of Microbiology. 2014; 45 (2): 509-514.

11

42. Massoud Hajia, Mohamad Rahbar, Marjan Rahnamye Farzami,

Hossein Masoumi Asl, Alireza Dolatyar, Mohsen Imani, Roghieh

Saburian, Moharam Mafi, Bita Bakhshi. Assessing clonal

correlation of epidemic Vibrio cholerae isolates during 2011 in 16

provinces of Iran. Curr Microbiol. 2015 Aug; 71(2):163-9.

43. Bakhshi B, Ghafari M, Pourshafie MR, Zarbakhsh B, Katouli M,

Rahbar M, Hajia M, Hosseini-Aliabad N, Boustanshenas M.

Resistance-Gene Cassettes Associated With Salmonella enterica

Genotypes. Lab Med. 2015 Spring;46(2):90-6.

44. Bakhshi B, Mahmoudi-Aznaveh A, Salimi-Khorashad A. Clonal

Dissemination of a Single Vibrio cholerae O1 Biotype El Tor

Strain in Sistan-Baluchestan Province of Iran During 2013. Curr

Microbiol. 2015 Aug;71(2):163-9.

45. Azam Mahmoudi-Aznaveh, Bita Bakhshi, Shahin Najar-

Peerayeh, Anoshirvan Kazemnejad, Zahra Rafieepour,

Mohammad Rahbar, Shahla Abbaspour. Commensal E. coli as an

Important Reservoir of Resistance Encoding Genetic Elements.

Int J Ent Pathog. 2013 November; 1(2): 44-8.

46. Mahsa Alizadeh-Hesar; Bita Bakhshi, Shahin Najar-peerayeh.

Molecular Diagnosis of Salmonella enterica and Shigella spp. in Stool

Sample of Children With Diarrhea in Tehran. Int J Ent Pathog.

2014 May; 2(2): e17002.

47. Derakhshan S, Peerayeh SN, Bakhshi B. Genotyping and

characterization of CTX-M-15 -producing Klebsiella pneumoniae

isolated from an Iranian hospital. J Chemother. 2015 Mar [Epub

ahead of print].

48. Alizadeh-Hesar M, Bakhshi B, Najar-Peerayeh S. Clonal

dissemination of a single Shigella sonnei strain among Iranian

12

children during Fall 2012 in Tehran, I.R. Iran. Infect Genet Evol.

2015 Jun 24. [Epub ahead of print]

49. Bita Bakhshi, Mohsen Ghafari, Zahra Rafiee pour, Malihe

Talebi. High efficacy of molecular methods for identification of

Salmonella enterica serogroups: A two year surveillance. Infect Dis

Clin Pract, 2015 (Accepted).

50. Majid Akbari, Bita Bakhshi, ShahinNajarPeerayeh. Specific

Distribution of Enterobacter cloacae Strains Isolated from UTI

within Clonal Complexes. Iran Biomed J. (Accepted)

51. Bita Bakhshi, Amin Naseri, Masoud Alebouyeh. Antimicrobial

susceptibility of Campylobacter strains isolated from patients with

diarrhea and food samples. Iran Biomed J, 2015 (Accepted).

52. Majid Akbari, Bita Bakhshi, Shahin Najar Peerayeh, Mehrdad

Behmanesh. Detection of curli biogenesis genes among

Enterobacter cloacae complex isolated from blood cultures. Int J Ent

Pathog, 2015 (Accepted).

Publications in Persian

ارسیاتی رضای آسهایطگای . ، اصز تاداهی، هحوذرضا خزهی ساد، سز پزربی تا بخشی -1

.20-15، صفحات (23) 1382. هجل تطخیص آسهایطگای. تطخیص عفتای هایکپالسوایی

آرتزیت راکتی یا آرتزیت عفی . ، هحوذرضا خزهی ساد، اصز تاداهی، هحوذ پشضکیبی تا بخشی -2

.84-71صفحات . (1) 1383. هجل طة تشکی. هشهي

13

گشارضی اس . ، حسیي اصزی، هحسي سزایی، هحوذتقی افطای، هحوذرضا پرضفیعبی تا بخشی -3

هجل . تیتیپ التر در ضوال ضزقی ایزاىO1اتطار کلال سی جذیذ یثزی کلزا سزتیپ

. 69-67صفحات . (2) 1387. هیکزب ضاسی پشضکی

تزرسی هلکلی . هحوذرضاپرضفیع هزداداضوی، ،تابخشی بی افسطزیف یا،- 4

. اسالهی آساد داطگا پشضکی علم هجل. سال در ایزاى5سزاپیذهیلصی تا طی یك هطالع

. 252-257. 20(4). 89سهستاى

تطخیص جذاساسی. جعفزی ، احوذجیذیتابخشی بیهحوذرضاسوائی، سیذتاقزهزتضی، - 5

درحذف آى کارایی تزرسی اس کوپست گشادکاى زهال کذ تجشی تاکتزی گ یك هلکلی

. 167-178. 41(4). 92سهستاى . یشد تذاضت پضطی داطکذ علوی فصلاهة. گشادکاى

در سی ای IS1004 پزفایل صتیکی. پرضفیع هحوذرضا یا، ضزیف افسى تابخشی، بی- 6

تار . گزهسیزی عفی تیواریای فصلاه. هحیطی یثزی کلزا جذا ضذ اس آتای سطحی درایزاى

92 ،60 .

ارسیاتی رضای آسهایطگای هلکلی تطخیص . ، ضیي جار پیزایبی تا بخشیهجیذ اکثزی، - 7

.(پذیزش)هجل کهص . عفت ای ادراری اضی اس اتزتاکتز کلآک

ارسیاتی رش ای سزتایپیگ هلکلی در . ضیي جار پیزای، بی تا بخشی ،اعظن هحودی اسا- 8

فصلاه تیواریای عفی . جذا ضذ اس کدکاى هثتال ت اسالکالی. ایضاسایی اتزپاتصیك

.(پذیزش)گزهسیزی

14

PRESENTATIONS

1. Bakhshi B.; Khorramizadeh M.R.; Badami N.; Pezeshki M.;

Gharibdoost F.(2003) Comparision of Culture and Polymerase

Chain Reaction (PCR) in Detection of Mycoplasmas in the Synovial

Fluid of Patients with Arthritis. The 5th Iranian Congress of Microbiology,

Jan. 21st- Jan. 23rd, Ahvaz, Iran. (oral presentation).

2. Bakhshi B.; Khorramizadeh M.R.; Badami N.; Pezeshki M.(2003)

The First Molecular Survey on Mycoplasmal Infections in

Inflammatory vs. Non-inflammatory Arthritis in Iran. 13th European

Congress of Clinical Microbiology and Infectious Diseases, Glasgow, UK.

3. Dabiri H.; Bakhshi B.; Badami N.; Korramizadeh M.R. (2004).

Detection of Mycoplasme DNA in Human Atherosclerotic Plaques

by PCR. The 6th Iranian Congress of Microbiology, Feb. 16st- Jan. 18rd,

Tehran, Iran.

4. Bakhshi B.; Dabiri H.; Badami N.; Korramizadeh M.R. (2006)

Detection of Mycoplasma and Chlamydia DNA in Human Synovial

Fluids and Atherosclerotic Plaques. 10th Western Pacific Congress of

Chemotherapy and Infectious Diseases (Poster Presentation), Japan.

5. Bakhshi B.; Pourshafie M.R.; Navabakbar F.; Tavakoli A. (2007)

Diversity in the Content and Arrangement of CTX Genetic Element

among Toxigenic Vibrio cholerae Strains Isolated during 2004-2006 in

Iran.17th European Congress of Clinical Microbiology and Infectious Diseases,

Munich, Germany.

6. Bakhshi B.; Pourshafie M.R.; Navabakbar F.; Tavakoli A. Study of

Genetic Diversity of Vibrio cholerae Strains Isolated from Patients

15

during 2004-2006 by Pulsed Field Gel Electrophoresis (PFGE) and

PhenePlate (PhP) Typing. 1th Iranian Congress of Clinical Microbiology,

Iran, Shiraz, 2007. (Oral presentation).

7. Adabi M.; Bakhshi B.; Pourshafie M.R.; Goudarzi H. Distribution of

class I integrons in different Vibrio cholera O-serotype strains isolated

during 1383-1385 from 4 Provinces of Iran. 1th Iranian Congress mof

Clinical microbiology, Iran, Shiraz, 2007.

8. Bakhshi B.; Pourshafie M.R.; Navabakbar F.; Tavakoli A. Southern

blot analysis of cholera toxin gene array among Vibrio cholerae strains

isolated in Iran. The Biology of Vibrios. Paris, France. 28 nov- 1 Dec 2007.

9. Mohammadi M., Bakhshi B., RastegarLari A.A., Pourshafie M.R.

Diversity of Vibrio Pathogenicity Island (VPI) in Clinical and

Environmental Isolates of Vibrio cholera from Iran. The Biology of

Vibrios. Paris, France. 28 nov- 1 Dec 2007.

10. Adabi M.;Bakhshi B.;Pourshafie M.R.; Goudarzi H. Distribution of

class I Integron and SXT Element in DifferentVibriocholerae O1

SerotypestrainsIsolatedduring 2004-2006 from Iran. The Biology of

Vibrios. Paris, France. 28 nov- 1 Dec 2007.

11. Mohammadi M., Bakhshi B., RastegarLari A.A., Pourshafie M.R.

Environmental Isolates of Vibrio cholerae as a Reservoir of Virulence

Associated Genes. II International Conference on Environmental, Industrial

and Applied Microbiology. 28th Nov-1th Dec. 2007, Seville, Spain. (Oral

presentation).

12. Adabi M, Bakhshi B, Goudarzi H , Pourshafie M.R. Molecular

Analysis of Antibiotic Resistance Conferred by a Class I Integron in

VibriocholeraeO1 Strains Isolated in Iran. 10thIranian Congress of

Microbiology, Kerman, Iran.

16

13. Mohammadi M., Bakhshi B., Rastegar Lari A.A., Masjedian F,

Dashtebani A, Pourshafie M.R. Genetic diversity of Toxin

Co-regulated Pilus gene cluster in Vibrio choleraeisolates of clinical

origin. 10thIranian Congress of Microbiology, Kerman, Iran. (oral

presentation).

14. Bakhshi B;Naseri H, Zahraei M, Afshani MR, Pourshafie MR.

Emergence and Clonal Dissemination of a New Genotype of Vibrio

cholerae Serotype O1 Biotype ElTor in Iran North- West in Summer

of 2007. The first Iranian congress on Enteric Pathogen, 2008, Sanandaj.

(oral presentation).

15. Sharifnia A., Bakhshi B., PourshafieMR.Characterization of

diversity in wbeT region of the O-antigen biosynthesis gene cluster in

Ogawa and Inaba serotypes of Vibrio cholerae. The first Iranian congress

on Enteric Pathogen, 2008, Sanandaj. (Oral presentation).

16. Bakhshi B;Pourshafie MR Assessing Clonality of Vibrio Cholerae

Strains Isolated During a 4 Year Period in Iran. 13th International

Congress of Infectious Disease Kuala Lumpur, Malaysia, June 19-22, 2008.

17. Pourshafie M.R., Bakhshi B. High molecular clonality of Vibrio

cholerae in Iran during the 10 years with a single predominant type in

years under study. 2th Enteric Pathogen Seminar, 9-10 Aban 1389,

Sanandaj, Iran.

18. Ghafari M., Bakhshi B., Pourshafie M.R., Amirmozafari N. Salimi

M., Rousta F. Investigation of genetic diversity of Salmonella

enterica strains isolated from patients by Pulsed Field Gel

Electrophoresis. Institut Pasteur International Network Annual Scientific

Meeting, Hong Kong, 22-23 November, 2010.

19. Najibi S., Bakhshi B., Sattari M., Alebouye M., Tajbakhsh M. Class

1 integrons and associated resistance gene cassettes among

17

enteropathogenic Escherichia coli.21th European Congress of Clinical

Microbiology and Infectious Diseases, 7-10 May, Milan, Italy.

20. Najibi S., Bakhshi B., Sattari M. Distribution of gene cassettes

dfrA7, dfrA1 and dfrA12 among enteropathogenic Escherichia coli

(EPEC) isolates. The 5th Iranian congress of Clinical Microbiology, 8-10

November, 2011, Shiraz, Iran.

21. Boustanshenas, M., Bakhshi, B., Ghorbani, M., Atyabi, M., Fazeli,

M.R., Norouzian, D. Recombinant system for expression of cholera

toxin B subunit from Vibrio cholerae in Escherichia coli. 1st International

and 12th Iranian Congress of Microbiology, 23-26 may, 2011, Kermanshah,

Iran.

22. Eftekhari, M., Bakhshi, B., Pourshafie, M.R., Amirmozafari N.,

Zarbakhsh, N. Distribution of class I & II integrons among

shigellaspp. 1st International and 12th Iranian Congress of Microbiology, 23-

26 may, 2011, Kermanshah, Iran.

23. Najibi, S., Bakhshi, B., SepehriSeresht, S. Class II integrons among

EnterohemorragicE.coli(EHEC)strains isolated from diarrheal stools.

1st International and 12th Iranian Congress of Microbiology, 23-26 may,

2011, Kermanshah, Iran.

24. Najibi, S., Bakhshi, B., SepehriSeresht, S., Sattari M. Distribution of

class II integrons among EnterohemorrhagicE.coli (EHEC) strains

isolated from diarrheal stools. 1st International and 12th Iranian Congress

of Microbiology, 23-26 may, 2011, Kermanshah, Iran.

25. Bakhshi, B. Molecular epidemiology and advanced techniques in

diagnosis of Vibrio cholerae. The first Iranian International Congress of

Medical Bacteriology, September 2011, Tabriz, Iran.

18

26. Bakhshi, B., Pourshafie, M.R., Bahmanyar, N. Biochemical

fingerprinting of Vibrio cholerae isolates. Vth World Aqua Congress,

November 2011, Dehli, India.

27. MahsaAlizadehHesar, BitaBakhshi, ShahinNajarPeerayeh.

Distribution of tetracycline resistance determinants among

Shigella strains isolated from patients with diarrhea in Fall 2012 in

Tehran,Iran. The Second Iranian Congress of Medical Bacteriology, 2-4

October 2013, Tehran, Iran.

28. MahsaAlizadehHesar, BitaBakhshi, ShahinNajarPeerayeh. High

level of homogeny among Shigella strains isolated from patients

with diarrhea in Fall 2012 inTehran,Iran. The Second Iranian Congress

of Medical Bacteriology, 2-4 October 2013, Tehran, Iran.

29. M. Kalantar, B. Bakhshi, F. Fallah.Campylobacter isolated from

chicken carcasses as in important reservoir virulence gene

determination. The Second Iranian Congress of Medical Bacteriology, 2-4

October 2013, Tehran, Iran.

30. AzamMahmoudi-Aznaveh, BitaBakhshi, ShahinNajar-Peerayeh.

The occurrence of dfra7 and dfrA1 resistance gene cassette within

class 1 integron among EPEC. The Second Iranian Congress of Medical

Bacteriology, 2-4 October 2013, Tehran, Iran.

31. AzamMahmoudi-Aznaveh, BitaBakhshi, ShahinNajar-Peerayeh.

Class two integrons with array within EPEC isolates in 2012. The

Second Iranian Congress of Medical Bacteriology, 2-4 October 2013,

Tehran, Iran.

32. Mina Boustanshenas ,BitaBakhshi, Majid Akbari. The

effectiveness of API strips and PCR method in compare with

biochemical tests for identification of Aeromonas spp. The Second

Iranian Congress of Medical Bacteriology, 2-4 October 2013, Tehran, Iran.

19

33. B. Bakhshi, M.Ghorbanalizadgan. Identification of

ThermophilicCampylobacter strains isolated from diarrheal

patients in Iran by cytolethal distending toxin (cdt) gene-based

multiplex PCR assay. The Second Iranian Congress of Medical

Bacteriology, 2-4 October 2013, Tehran, Iran.

34. NasimAfshari, BitaBakhshi ,FatemeFallah. Genotyping pattern

of Shigella isolate using ERIC-PCR analysis. The Second Iranian

Congress of Medical Bacteriology, 2-4 October 2013, Tehran, Iran.

35. Majid Akbari, BitaBakhshi, ShahinNajar-Peerayeh. Specific

distribution within the Enterobacter cloacae complex of Strains

Isolated from Urinary Tract Infection in Tehran. The Second Iranian

Congress of Medical Bacteriology, 2-4 October 2013, Tehran, Iran.

36. BitaBakhshi, NazaninEftekhari. PFGE analysis of Shigellaspp.

isolated from patients with diarrhea. XXIII International ICFMH

Symposium, FoodMicro 2012, Istanbul, Turkey.

37. Bita Bakhshi. Genetic diversity of V. cholerae and detection of El

Tor Variant strains during 2012 cholera outbreak. MEMBS 2014,

Dubai, United Arab Emirates.

20

COURSES/ THOUGHT

1. PhD level, Toxins and different bacterial components.

2. PhD level, Medical Bacteriology

3. PhD level, The role of bacteria in medical biotechnology

4. PhD level, Advanced genetic of bacteria

5. PhD level, Bioinformatics

6. PhD level, Advanced bacteriology

7. MSc level, Molecular cell biology of eukaryotes and prokaryotes

8. MSc level,Host-parasite interactions

9. MSc level, Structure and physiology of Microorganisms

10. MSc level, Systematic bacteriology

11. MSc level, Molecular diagnostic bacteriology

THESIS, SUPERVISED OR ADVICED

1. Supervised, (Mina Boustanshenas, MSc).

2. Supervised, ( NazaninEftekhari,MSc).

3. Supervised, (Mohsen Ghafari, MSc).

4. Supervised, (Amin Naseri, MSc).

5. Supervised, (SoheilFalahzad, MSc).

6. Supervised, (NedaHosseini, MSc).

7. Supervised, (SakinehNajibi, MSc).

8. Supervised, ( MehdiGhorbanalizadegan, PhD).

9. Supervised, ( MajidAkbari, PhD).

10. Supervised, (AzamMahmoudiAznaveh, MSc.).

11. Supervised, (MahsaAlizadehhesar, MSc.).

12. Advised, (MasoumehNavidinia, PhD).

21

13. Advised, (SafouraDerakhshan, PhD).

14. Advised, (Mohammad Reza Samai, PhD).

15. Advised, (Maryam Roudbari, PhD).

16. Advised, (KobraSalimian, MSc)

17. Advised, (AbdolmajidGhasemian, MSc)

18. Advised, (Maryam Hosseinipour, MSc)

19. Advised, (Ali JazayeriMoghadas, PhD)

DEPOSITS IN GENEBANK

Pseudomonas aeruginosa strain Darab1 16S ribosomal RNA gene, partial sequenceAccession: JQ397281.1 GI: 379047303 Pseudomonas aeruginosa strain Darab2 16S ribosomal RNA gene, partial sequenceAccession: JQ397282.1 GI: 379047304 Acinetobacterradioresistensstrain Abadan 16S ribosomal RNA gene, partial sequence Accession: JQ397280.1 GI: 379047302 Vibrio cholerae strain CIP104154 hemolysin (hlyA) gene, partial cds Accession:GU809236.1GI:294715428 Vibrio cholerae strain Env18Q hemolysin (hlyA) gene, partial cds Accession:GU809234.1GI:294715424 Vibrio cholerae strain Env18Q aspartate-semialdehyde dehydrogenase gene, partial cds Accession:GU321114.1GI:284157087 Vibrio cholerae strain Env25 aspartate-semialdehyde dehydrogenase gene, partial cds Accession:GU321112.1GI:284157083 Vibrio cholerae strain CIP104154 aspartate-semialdehyde dehydrogenase gene, partial cds Accession:GU321110.1GI:284157079

22

Vibrio cholerae strain Env8 aspartate-semialdehyde dehydrogenase gene, partial cds Accession:GU207846.1GI:281313009 Vibrio cholerae strain Env40 hemolysin (hlyA) gene, partial cds Accession:GU809235.1GI:294715426 Vibrio choleraestrain Env10Q aspartate-semialdehyde dehydrogenase gene, partial cds Accession:GU321113.1GI:284157085 Vibrio cholerae strain Env34 aspartate-semialdehyde dehydrogenase gene, partial cds Accession:GU321111.1GI:284157081 Vibrio cholerae strain ATCC 14035 aspartate-semialdehyde dehydrogenase gene, partial cds Accession:GU207847.1GI:281313011 Vibrio cholerae strain 2132 WbeT gene, partial cds Accession:FJ619106.1GI:222822579 Escherichia coli strain 75p class 1 integron, partial sequence Accession:JN870738.1GI:378812709 Escherichia coli strain 43p class 1 integron, partial sequence Accession:JN870739.1GI:378812713 Escherichia coli strain 58p class 1 integron, partial sequence Accession:JN870737.1GI:378812708 Vibrio cholerae strain CIP 104154 recombinaseA (recA) gene, partial cds Accession:FJ479705.1GI:215254338 Vibrio cholerae isolate Env8 recombinaseA (recA) gene, partial cds Accession:FJ479703.1GI:215254334 Vibrio cholerae strain ATCC 14035 recombinaseA (recA) gene, partial cds Accession:FJ479704.1GI:215254336 Vibrio cholerae isolate Clin2200 recombinaseA (recA) gene, partial cds Accession:FJ479702.1GI:215254332 Escherichia coli strain 2p class I integron aminoglycoside adenyltransferase (aadA1) gene, complete cds

23

Accession:JN815078.1GI:356892455 Vibrio cholerae strain 65-1384 CtxB (ctxB) gene, ctxB-classic allele, complete cds Accession:JN132472.1GI:356494216 Vibrio cholerae strain 65 WbeTtransferase (wbeT) gene, partial cds Accession:JN561165.1GI:344330760 Vibrio choleraestrain Env31Q recombinaseA (recA) gene, partial cds Accession:FJ645930.1GI:223047501 Vibrio cholerae strain Env34 recombinaseA (recA) gene, partial cds Accession:FJ645929.1GI:223047499 Vibrio cholerae strain Env40 recombinaseA (recA) gene, partial cds Accession:FJ224374.1GI:209922123 Vibrio cholerae strain ATCC 14035 WbeT (wbeT) gene, partial cds Accession:FJ593961.1GI:220682211 Vibrio choleraestrain Env25 hemolysin (hlyA) gene, partial cds Accession:GU593974.1GI:292385817 Vibrio cholerae strain Env34 hemolysin (hlyA) gene, partial cds Accession:GU586281.1GI:291170721 Vibrio cholerae strain Env8 hemolysin (hlyA) gene, partial cds Accession:GU586279.1GI:291170717 Vibrio cholerae strain Env31Q hemolysin (hlyA) gene, partial cds Accession:GU586277.1GI:291170713 Vibrio cholerae strain Env4Q hemolysin (hlyA) gene, partial cds Accession:GU593975.1GI:292385819 Vibrio cholerae strain ATCC 14035 hemolysin (hlyA) gene, partial cds Accession:GU586280.1GI:291170719 Vibrio cholerae strain Clin2200 hemolysin (hlyA) gene, partial cds Accession:GU586278.1GI:291170715

24

Vibrio cholerae strain Clin2200 aspartate-semialdehyde dehydrogenase (asd) gene, partial cds Accession:GU190716.1GI:270312214 Vibrio cholerae strain Env31Q aspartate-semialdehyde dehydrogenase (asd) gene, partial cds Accession:GU190715.1GI:270312212