Ribosome

Lectures: 1. Types and roles of RNA2. Structure and function of RNA3. Regulation of mRNA translation4. Non-coding RNA5. Riboswitches and Ribozymes 6. RNA in biotechnology

Labs: 1. Targeting an RNA of interest2. Chemical probing extracted RNA 3. Chemical probing in-cell RNA4. High-throughput sequencing5. Chemical reactivity analysis6. Modeling RNA structures

BIT 495/595 RNA World

Structure Function

Secondary structure

RNA

Higher-order structure

Ribozyme

Riboswitch

RNA molecules have a diverse range of functions.

Many RNA functions depend on secondary and higher-order structure.

• Protein synthesis mRNA, rRNA, tRNA

• Regulatory RNA e.g. lncRNA, miRNA

• DNA replication & post-transcriptional modification

e.g. snRNA, smYRNA

• Parasitic RNAe.g. Viral genome, viroid, retrotransposons.

tmRNA SRP RNA

IRES Transfer RNA

snRNA