biotechnology research in kenya dr. eliud kireger director ...€¦ · molecular markers assisted...
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Kenya Agricultural & Livestock Research Organization
Biotechnology research in Kenya Dr. Eliud Kireger
DIRECTOR GENERAL
Presentation Outline
1. About KALRO
i. Mandate
ii. Directorates
iii.Institutes
iv.Biotechnology research in Kenya
The Kenya Agricultural and Livestock Research Organization (KALRO) was created through the KALR Act of January 2013 and established on 1st July 2014.
The Act merged the former Kenya Agricultural Research Institute (KARI), Coffee Research Foundation (CRF), Tea Research Foundation of Kenya (TRFK) and Kenya Sugar Research Foundation Sugar (KESREF). The four institutes form KALRO.
KALRO’s Mandate To promote, streamline, co-ordinate and regulate research in
crops, livestock, genetic resources and biotechnology and animal diseases;
To expedite equitable access to research information, resources and technologies and promote the application of research findings and developed technologies in the field of agriculture. And livestock
KALRO has 2 Research and 1 Corporate Services Directorates, 16 Institutes, 51 Centres & Sub-centres strategically located in the country to enable the organization meet farmers demands throughout Kenya varied agroecological zones.
KALRO’s organizational
structure
Corporate Services Directorate
Overall Objective
To support the research directorates
Crops Research Directorate Programme
Overall Objective
To generate & disseminate knowledge, technologies and innovations for enhanced productivity, value addition, profitability & competitiveness of food, horticultural & industrial crops, tea, coffee and sugar.
Livestock Research Directorate Programme
Overall Objective
To Develop and promote knowledge, information and technologies/innovations that enhance livestock productivity, value addition and product development, and facilitating market access.
KALRO’s Crops Research
Institutes
Coffee Research Institute Mandate
The Institute conducts research in all areas of production, processing and marketing.
Food Crops Research Institute
Mandate
To generate and disseminate food crops' knowledge, innovative technologies and services that respond to clientele demands
Horticulture Research Institute
Mandate
To conduct research in horticultural crops covering vegetables, fruits, flowers and disseminate appropriate information and technologies.
Industrial Crops Research Institute Mandate To generate and disseminate Industrial crops knowledge, innovative technologies and services
Sugar Research Institute Mandate To promote research and investigate all problems related to sugarcane and such other crops, processing into sugar and by-products, and their systems of husbandry, productivity, quality and sustainability of land
Tea Research Institute Mandate
Development of improved clones, appropriate technologies for improvement of yield and quality of tea products.
KALRO’s Livestock Research
Institutes
Beef Research Institute Mandate
Focuses on improving productivity of beef cattle.
Apiculture Research Institute Mandate
The Apiculture Research Institute will address the challenges affecting the Apiculture sub sector and promote value addition along its value chain.
Dairy Research Institute
Mandate
Conduct research on dairy cattle, goats and camels, generate knowledge and develop technologies and disseminate to users.
Non-Ruminant Research Institute
Mandate
To generate non-ruminant knowledge, innovative technologies and disseminate to farmers.
Sheep , Goat and Camel Research Institute
Mandate
To generate and promote technologies and innovations for demand-driven sheep and goat product value chains nationally
Veterinary Research Institute Mandate
Research is geared towards alleviating the impact of livestock diseases to enhance livestock production.
KALRO’s Cross-cutting
Research Institutes
Arid & Range Lands Research Institute Mandate To address challenges in dry lands by developing technologies for re-vegetation and restoration of degraded rangelands and enhance ASAL livestock production and dry land farming.
Biotechnology Research Institute
Mandate
To generate and promote agricultural & livestock biotechnologies and innovations to support the sectors.
Mandate To generate knowledge, technologies and innovations for effective conservation and utilization of plant, animal and microbial genetic resources
Genetic Resource Research Institute
Agricultural Mechanization Research Institute
Mandate
Development of appropriate Agricultural mechanization technologies for improvement of efficiency and in farming.
Machakos
Trans Nzoia
Bungoma
Busia
Kakamega
Nairobi
Kiambu
Kisumu
Kericho
Kisii
Homabay Muranga
Embu
Kirinyaga
Taita Taveta
Garisa
Baringo
Marsabit
KALRO Research Institutes and Centres
Status of Agricultural Biotechnology in Kenya
30 30
Emerging challenges to Agricultural Production that requires Biotechnology approaches
Droughts
Floods
Changes in rainfall patterns
Increased temperatures
Floods
Source - CIMMYT
Climate Change
• Environment degradation
• salinity
• In Kenya five main Biotechnology tools are used
Livestock and crop improvement
1. Tissue Culture
2. Molecular Markers- assisted breeding
3. Development of Genetically Modified Crops
4. Plant and animal disease diagnostics
5. Development of livestock diseases recombinant
vaccines
Examples of Modern Biotechnology in Kenya
Tissue Culture (TC)
Main application for TC has been micro-propagation of clean planting materials of vegetatively propagated crops for farmers
•TC started in Kenya in the early 80s for mass propagation of pyrethrum
•Tissue Culture Banana – Has been seen as a major biotechnology success story
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Tissue culture and its application in
mass propagation of improved crops
Tissue culture bananas
Other tissue culture crops
Other tissue culture crops
Other tissue culture crops
Molecular Markers Assisted Breeding
• Allows for shortening of the breeding to release new varieties
• Enables breeders to handle difficult crops whose time to maturity may be too long eg. Coconut, cashewnuts, mangoes
• Allows breeders to select for traits that may change with different environments e.g sweet potato flesh colour at different altitudes
40
Progress in use of Molecular Marker Assisted
Breeding
Characterization and mapping
of Maize Streak Virus and Grey
Leaf Spot resistance genes in
maize
Development of drought
tolerant maize and wheat
varieties
Development of Russian
Wheat Aphid resistant wheat
lines
Fingerprinting of maize land
races
Diversity Studies on
Sweetpotato, Cassava,
Dolichos and Aloe vera
Characterization of
Indigenous breeds of
cattle poultry, forages
and tsetse population
genetics
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Development of GM crops in Kenya
Crop Status
Cotton CFT
Maize CFT
Cassava CFT
Sorghum CFT
Gypsophila CFT
Sweetpotato CFT Approved
Groundnuts Lab/Green house
Pigeon pea Lab/Greenhouse
Rice Lab/Greenhouse
Banana Lab/Greenhouse
Rice Lab/Greenhouse
Progress on Bt Maize research Location of Biotech Crops CFTs in Kenya
WESTERN KENYA (Busia)
Virus Resistant Cassava
(VIRCA) – KALRO Alupe
CENTRAL KENYA
Bt cotton - KALRO Mwea
Research Centre
NAIROBI
Drought Tolerant Maize
Sweet Potato Action for Security and
Health in Africa (SASHA)
Improved Maize for African Soils
(IMAS) – KALRO Biotechnology Center
LOWER EASTERN
KENYA KALRO Kiboko
Research Centre
Water Efficient Maize for Africa
(WEMA), Africa Biofortified
Sorghum (ABS) and Insect
Resistant Maize COAST REGION
Virus Resistant Cassava for Africa (VIRCA) –
KALRO Mtwapa Research Centre Developed by ISAAA AfriCenter, February 2013
Progress on Bt Maize research
• We are currently doing a Stack event that contains both Insect resistance and drought tolerance traits
• CFT planting at Kitale carried out on 26th Apr., 2016 and at Kiboko carried out on 6th May, 2016
Progress on Bt Maize research
• Why Kiboko? – To generate field data on resistance to Spotted stemborer (Chilo partellus)
Progress on Bt Maize research
• Why Kitale? – To generate field data on African stemborer, (Busseola fusca)
Stack maize testing CFT I, KALRO Kitale
Leaf damage on stack event MON810XMON87460 and Non-Transgenic isolines
maize plants after infestation with Busseola fusca stem borer
Transgenic maize Non-Transgenic maize
Progress on Bt Maize research
• KALRO and AATF received approval from NBA to carry out Bt (MON810) maize testing under the National Performance Trials (NPT) in early April, 2016
• EIA/EISA project report generated by NEMA accredited Experts working with KALRO and AATF scientists and submitted to NEMA
Progress on Bt Maize research
• 6 KEPHIS designated sites are under development (Embu, Mwea, Thika, Kibos, Kakamega, and Alupe) were selected for testing
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Other areas of Biotechnology application in Kenya’s Crop agriculture
49 Drought
Pests
Maize Lethal
Necrosis Disease Cassava:
cyanide; viruses
Crop Diseases
& pests
Crop improvement
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Biotechnology and its application in Kenya: Animal Production – Embryo Transfer (ET)
Tool for rapid multiplication of animal germplasm with desired genetic traits
Disease tolerant breeds
Dairy breeds for milk production
KALRO & ADC ET laboratories
equipped & operational; Training on-
going; Private Dairy Farms using ET
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Microscopy;
low sensitivity
Enzyme-Linked
Immunosorbent
Assays (ELISA);
results in 2-4 hours
Capri test kit: Rapid
Diagnostic Test for CCPP
(2-3 minutes)
Animal Health: Disease Diagnosis
Biotechnology presents opportunity to
develop Rapid Diagnostic Tests for disease
diagnosis using genomic approaches
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Biotechnology in Animal Nutrition
Improvement of Pastures / fodder
Opuntia high in nutrients, therefore, alternative livestock feed in ASALs
Way forward for successful biotechnology application in Kenya
Research Infrastructure
Human capacity (balance of appropriate skills and numbers)
Financial resource
Regulatory systems
Political goodwill
Strong policy/legal framework
Enhance effective partnerships along agricultural product value chains
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Acknowledgements National Institutions: NBA, KEPHIS, NEMA, DVS, KEBS, Universities, Others International Centres: CIMMYT, ISAAA, NEPAD/ABNE, PBS, AATF, OFAB, ABSF, Africa Harvest, Others Donor Community – BMGF, HBF, USAID, McKnight Foundation, Others
Thank You & God Bless You
Demand driven research for food security and income generation
Demand driven research for food security and income
generation