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ChitosanActive

Biostimulant

100Natural

From shrimp extract Sticks to the plant creating a nurturing layer Fast entry and assimilation via leaves High activation of its efficacy due to molecular optimisation

With

www.kaitosol.comwww.kaitosolglobal.com

For use in all type of crops

Chitosan Active

Biostimulant

The University of Cambridge

100Natural


With

Developed at


Chitosan Active

Biostimulant


100Natural


With

Developed at


ChitosanActive

Biostimulant


100Natural


With

Developed at


Chitosan Active

Biostimulant


100Natural


With

Developed at


Chitosan Active

Biostimulant


100Natural


With

Developed at


KaitoSol® is a foliar biostimulant which is 100 % natural. It is based on ActiveChitosan, a molecule made from plant and shrimp extracts.

ActiveChitosan is the main active ingredient in KaitoSol®. It was developed at The University of Cambridge. It has an optimised structure and chemi-cal composition when compared to regular chitosan, creating an improved interaction with the plant. The optimised size and uniformity of the molecule creates a highly active compound.

- NEVER mix with pesticides. Mix only with water.- Do not apply two days before and/or after a herbicide application.- FOLIAR application. It can be applied through manual or mechanical means.- Mix with enough water to completely cover the area to treat.- Shake product before use.- Do not add to the tank when completely empty or completely full.

General application procedure and recommendations (It is important to mention that KaitoSol® is not a replacement of standard fertilizers and should be used as an addition to standard

practices. However, it has been noticed that the use of KaitoSol® might reduce usage of pesticides and fertilizers).

ChitosanActive

2.- It has high adherence properties, creating a nurturing layer, - and it acts as a natural barrier against external agents

3.Since the molecule remains attached to the plant for an extended period, there is good absorption and small loss of active material. ActiveChitosan, as it is absorbed, enhances the strength of the plant and provides to the plant nutrients.

4.What happens:- Increases yield- Reduction in growth time- Aids on preventing disease- Enhances the strength of the plant- Induces chitin & phytoalexin enzymes

How KaitoSol® works

1.KaitoSol® is applied to the leaves and trunk of the plants via spray.

Adherence

Nurturing layer

Natural barrier against external

agents

Absorption

Activity

RegularChitosanChitosanActive

Examples of Product BenefitsWhere: Tolima, ColombiaVariety: ChontoProduction before applying KaitoSol®: 29 ton/HaType of application: foliar and dripResults: Increase in 30% of the mass of the tomatoes by using foliar application. If only using drip application, the increase in mass of each tomato was 25%. Drip application will require approximately twice as much KaitoSol® to produce similar results as foliar application.

Main Application ProcedureOnly mix with water. Shake product before use.

Rice

FieldTomatoes

Examples of Product BenefitsWhere: Tolima, ColombiaVariety: Fedearroz 60Production before applying KaitoSol®: 6 ton/HaType of application: foliar Results: An increase in 1000 kg per hectare. The pictures below are a snapshot before harvesting.

WithKaitoSol®

WithoutKaitoSol®


Analysing all tests done in rice worldwide, an average increase of 1.25 ton/Ha has been recorded. KaitoSol® has been used in different types of soils with efficacies varying from 2 tons/Ha to 10 tons/Ha (see graphs).

40%40% 40%

22%22% 22%

64%64% 64%

0%

20%

40%

60%

80%

100%

Average Minimum Maximum

Yiel

d In

crea

se %

1.25 1.25 1.25 1.25

1.04 1.04 1.04 1.04

1.45 1.45 1.45 1.45

0.80

1.00

1.20

1.40

1.60

Average Minimum Maximum

Yiel

d In

crea

se in

To

ns p

er H

ecta

re

Fourth

Second

TREATMENTS WHEN DOSAGE Per Ha

First

Third

Spray 20 days after sowing

1 L

0.5 LSpray 40 days after sowingSpray 60 days after sowingSpray 80 days after sowing

Fourth

Second

TREATMENTS WHENDOSAGE

Per 10,000 plants

First

Third

Spray the soil after sowing the seed

Spray the plant two weeks after the second applicationAfter transplanting, spray the soil and the plant

1 L

2 LFifth and following Apply once a month until harvesting

Spray the plant two weeks after the third application

Examples of Product BenefitsWhere: Galicia, SpainType of crop: ForageProduction before applying KaitoSol®: 40 ton/HaType of application: foliar Results: An increase of 10 tons per hectare, an increase in production of over 25%.

Main Application Procedure

Maize

Sugar CaneExamples of Product BenefitsWhere: Pereira, Colombia (Ingenio de Risaralda)Production before applying KaitoSol®: 110 ton/HaType of application: foliar Results: An increase of 44 ton per hectare of sugar cane and an increase of 4 tons per hectare of sugar.


Fourth

Second


First

Third

Spray 20 days after sowing 1 L Spray 35 days after sowingSpray 50 days after sowingSpray 65 days after sowing

Fourth

Second


First

Third

Spray when the plant is around 10 cm in height

Spray 10 days after the 3rd spraySpray 10 days after the 2nd spraySpray 10 days after the 1st spray

0.5 L

1 L

2 L

Potatoes

Flowers

Examples of Product BenefitsWhere: Tolima, Colombia.Variety: Pastusa.Production before applying KaitoSol®: 24 ton/Ha.Type of application: foliar, biweekly (2 L/Ha).Results: 14 tons/Ha increase (78% increase in yield). An increase in 35% of the weight of each potato and an increase in 32% in the number of potatoes per hectare. Phytophthora infestans presented itself, but it was treated with a fungicide. Therefore, the improvements seen in the trial are attributed to biostimulation.


Examples of Product Benefits

Plant vigour has increased and a reduction of infections has been seen. In ornamentals, we have obtained a reduction in growth time by approximately 15%.

Main Application ProcedureOnly mix with water. Shake product before use. Spray approximately 1 meter away from the plant.

TREATMENTS WHENRatio of

KaitoSol® to water

400 mL per 100 liters of

water

Through the entire life cycle of the plant

Spray plant weekly

Second and followings (until

harvest)


First Spray soil after sowing 1 L

Spray plant every two weeks 2 L

Examples of Product BenefitsThe product has been used and tested in crops such as brassicas, lettuces, onions, carrots and others. The main benefits seen have been an increase in yield and decrease in disease.

Main Application ProcedureOnly mix with water. Shake product before use. The applica-tion procedure described below is just for guidance. For a specific application procedure for your crop, please contact your distributor.

Examples of Product BenefitsThe product has been used and tested in crops such as banana, oranges, kiwis, strawberries and cherries. The main benefits seen have been an increase in yield and decrease in disease.

Main Application ProcedureOnly mix with water. Shake product before use. The application procedure described below is just for guidance. For a specific application procedure for your crop, please contact your distributor.

FruitsGeneral

FieldVegetables


KaitoSol® to water

Spray plants bi-weekly.

Spray plants monthly.

First four treatmentsAfter the fourth

treatment and for the rest of the life cycle of

the plant


water

TREATMENTS WHEN

Spray plant bi-weekly or weekly depending on the length of the life cycle of the plant.

Treat during the entire life cycle of the plant 1 L

DOSAGE Per Ha

Examples of Product BenefitsThe product has been used and tested in crops such as brassicas, lettuces, onions, carrots and others. The main benefits seen have been an increase in yield and decrease in disease.

Main Application ProcedureOnly mix with water. Shake product before use. The applica-tion procedure described below is just for guidance. For a specific application procedure for your crop, please contact your distributor.

Examples of Product BenefitsThe product has been used and tested in crops such as banana, oranges, kiwis, strawberries and cherries. The main benefits seen have been an increase in yield and decrease in disease.


FruitsGeneral

FieldVegetables


KaitoSol® to water

Spray plants bi-weekly.

Spray plants monthly.

First four treatmentsAfter the fourth

treatment and for the rest of the life cycle of

the plant


water

TREATMENTS WHEN

Spray plant bi-weekly or weekly depending on the length of the life cycle of the plant.

Treat during the entire life cycle of the plant 1 L

DOSAGE Per Ha

General Application ProcedureThe table below shows a general guidance of application frequency and dosage for the various types of crops. If your crop is not detailed in this brochure, please contact your distributor for a detailed application procedure.

Examples of Product BenefitsThe product has been used and tested in crops such as cucum-bers, red peppers, chili peppers and tomatoes. The main benefits seen have been an increase in yield and decrease in disease.


Greenhouse Vegetables

General

Fourth to Seventh


KaitoSol® to 100 L of water

800 mL

First*Second*Third**

Eigth until end of harvest

Spray soil after sowing

Spray once a monthSpray the plant every two weeks for two monthsAfter transplanting, spray the soil and the plant

Spray plant two weeks after sowing

400-800 mL per 100 liters of

water

TYPE OF CROP

Monthly300 mL per 100 liters of water

Lettuce, tomatoes, cucumbers

Trees or similar

Apply 3 to 4 times in the first 2 to 3 months of the

crops

Medium cycle crops

EXAMPLE DOSAGE FREQUENCY TYPE OF APPLICATION

Fruit trees, palm oil

Foliar

Foliar

Foliar

Greenhouse vegetables

5 to 15 days interval depending on the crop’s

life cycle

Rice, corn, wheat, etc.

0.5 - 2 L/Ha

*If you don’t have access to the plant at the propagation stage, ignore these applications.**If you don’t have access to the plant at the propagation stage, start treatments at this stage.

400 mL

of 2

ATTENTION This technical data sheet is provided for general information only

This biostimulant may not yet be available for sale or use in your area. It is your responsibility to follow government and local laws and regulations regarding the use of biostimulants. Before using the product, be sure the intended use is approved in your locality. Your locality may require additional precautions and instructions for use of this product that are not included here. Agentra does not guarantee the completeness or accuracy of this data sheet. The information found in this data sheet may differ from

the information found on the product label. You must have the government approved labelling with you at the time of use and mustread and follow all label directions.

You should not base any use of a similar product on the precautions, instructions for use or other information you find here. Always follow the precautions and instructions for use on the label of the product you are using.

A Biostimulant with ActiveChitosan® FOR USE IN ALL CROPS

•100 % natural •Increases yield •Strengthens the defencesystem of the plant •Helps plant growth •Helps seed

germination •Promotes growth of beneficial microorganisms

CHEMICAL COMPOSITION

Chitosan…………………….……………………….12.5 g/L Potassium - (K2O)………………………………...... 1.8 g/L

PHYSICAL APPAREANCE

Appearance…………………….…………………...Liquid Colour….…………………………………………….Brown Odour…………………….……………………..….Slight vinegar Solubility………………………………….. 100% soluble in water

PRECAUTIONARY STATEMENTS This products is not dangerous to health as defined by

the European Union dangerous substances /preparation directives

FIRST AID

Eye contact

May cause irritation. Will tend to form film on the surface of the eye causing blurred vision. Flush with plenty of water. Seek medical help if irritation persists

Skin contact

Can cause irritation rash. Wash with plenty of water. Seek medical help if irritation persists.

If inhaled Avoid breathing any fumes. Acute inhalation symptoms unknown. Move to fresh air and get medical help if persistent irritation or breathing difficulties occur.

Ingestion If swallowed no specific intervention is required as the compound is not likely to be hazardous by ingestion. However, consult nearest medical centre.

PERSONAL PROTECTIVE EQUIPMENT & HANDLING

No special equipment is necessary. See MSDS section 7 and 8.

USER SAFETY RECOMMENDATIONS User should wash hands before eating, drinking, chewing gum,

using tobacco, or using the toilet. User should follow all advice detailed in the MSDS of this

product

GENERAL DESCRIPTION OF PRODUCT The product is a concentrated liquid solution based on a proprietary molecule ActiveChitosan® and natural extracts. It is a natural product without genetic modifications or harmful chemicals. It is created by a fermenting process. Its principal benefits are: to increase yield by enhancing chlorophyll production and nutrient absorption; to strengthen the defence system of the plant; to help plant and root growth; to help seed germination; and to promote the growth of beneficial microorganisms.

DIRECTIONS FOR USE It is a violation of government law to use this product in a manner inconsistent with its labelling.

GENERAL USE PRECAUTIONS Do not mix with any pesticides. Do not mix with herbicides The product can be mixed with fungicides and insecticides but mixing and application compatibility must be tested locally prior to full scale use

USE PROMPLTY Use the product within two days of mixture. Reseal open bottles to avoid spillage. Consume open bottle within a 60 days of open.

MIXING INSTRUCTIONS Mix the product as follows (mix with glue-coadjutants if rain is an issue) Step 1: Add water to the mix tank (receptacle) and provide gentle agitation. Do not add the product to an empty or full tank. Step 2: Agitate the bottle of the product vigorously for a few minutes before mixing. Step 3: Add the required amount of the product. Please refer to the product’s brochure to determine required amount of the product. Step 4: Mix or agitate mixture until an even mixture is obtained. Avoid excessive foaming.

APPLICATION INSTRUCTIONS For the application procedure and the amount of product to be used per hectare on your specific crop, refer to the product’s brochure. GENERAL APPLICATION PROCEDURE: If the application procedure for your crop is not available in the brochure, contact Agentra for advice. For a general guideline, please see the table below. After planting, allow the plants to germinate and then start application. Application should be to the leaves and stem. Do not spray the soil unless specified otherwise in the product’s brochure. Automated, aerial or manual systems for irrigation can be used.

of 2

CROPS AND APPLICATION PROCEDURE

Example Dose Frequency

Trees or similar

Fruit trees, palm oil

200-400 mLper 100liters ofwater

Monthly

Greenhouse vegetables

Lettuce, tomato,

cucumber

400- 800 mLper 100liters ofwater

5 to 15 days interval

depending on the crop’s life

cycle

Medium cycle crops

Rice, corn, wheat, etc. 0.5 - 2 L/Ha

Apply 3-4 times in the

first 2-3 months of the

crop

PACKAGING 500 mL, 4 L and 20 L white plastic bottles. 1000 L under specific request

STORAGE AND DISPOSAL Do not contaminate water, food or feed by storage and disposal

PRODUCT STORAGE Keep product away from exposure to air, sunlight, moisture, or heat. Do not store in temperatures over 40 oC for more than seven days. Use product within 60 days of opening and within two days of mixing. The product has a two year shelf life.

DISPOSAL Wastes resulting from use of this product may be disposed of on site or at an approved waste disposal facility.

CONTAINER DISPOSAL Refillable container. Clean vigorously with water before reusing. If disposing, dispose container in a sanitary landfill or by incineration, or if allowed by government law and local authorities, by burning. If burned, stay out of smoke.

REGISTRATIONS FOR IMPORT AND SALES United Kingdom under tariff number 3101000000 Malaysia (country of origin) Indonesia under number L147/ORGANIK/PPI/X/2007 Cambodia under number 0227/1208 BGC Spain: Order APA/1470/2007

KaitoSol® is a registered trademark ActiveChitosan® is a registered trademark

AGENTRA is the exclusive importer and distributor of KaitoSol® to Europe, Latin America and Pakistan.

WARRANTY AND DISCLAIMER

CONDITIONS OF SALE AND EXCLUSIVE WARRANTY

The Directions for Use of this product reflect the opinion of experts based on field use and tests. The directions are believed to be reliable and must be followed carefully. However, it is impossible to eliminate all risks inherently associated with the use of this product. Crop injury, ineffectiveness of the product, or other unintended consequences may result because of such factors as horticultural practices, use with other products (unless otherwise expressly provided in the Directions for Use of this product), presence of other material, the manner of user or application, and other various environmental conditions, including but not limited to weather conditions and moisture conditions. To the extent consistent with applicable law, all of the aforementioned risks are beyond the control of Agentra and the Buyer shall assume all such risks.

EXCLUSIVE WARRANTIES Agentra warrants that at the time of first sale of this product it conforms to the chemical description on the label and under normal conditions is reasonable fit for the purposes referred to in the Directions for Use, subject to inherent risks referred above. To the extent consistent with applicable law, if this product does not perform as warranted above, customer’s sole remedy for breach of that warranty shall be replacement of the product or refund of the purchase price paid, at the option of Agentra. To the extent allowed by law, the foregoing warranties and remedies are exclusive and are in lieu of all other warranties, terms, or conditions expressed or implied, either in fact or by operation of law, statutory or otherwise, including but not limited to warranties, terms, or conditions o merchantability, fitness for particular purpose, and satisfactory quality.

LIMITATION OF LIABILITY To the full extent allowed by law, Agentra excludes for itself and its suppliers any liability, whether based in contract or tort (including negligence), for incidental, consequential, indirect, special, or punitive damages of any kind, or for loss of revenue or profits, loss of business, or crop injury arising out of or un connection with the sale, use, injury, performance, or failure of this product, even if Agentra or its authorised reseller has been advised of the possibility of such damages, and limits its liability to replacement of the product or a refund of the purchase price paid, at the option of Agentra. This disclaimer of liability for damages will not be affected if any remedy provided herein fails of its essential purpose.

ASSUMPTION OF RISK To the extent consistent with applicable law, Buyer and Users of this product assume the risk of any use contrary to the Directions for Use. Agentra offers this product, and the Buyer and User accept it, subject to foregoing Conditions of Sale and Exclusive Warranty, which may be varied only by agreement in writing signed by a duty authorised representative of Agentra.

PROMPT NOTICE OF CLAIM Agentra must have prompt notice as soon as a Buyer or User has reason to believe they may have a claim (not to exceed twenty-one days from date of application) so that an inspection of the affected property and growing crops may be made. To the extent consistent with applicable law, unless Buyer and Users promptly notify Agentra of any claims, they shall be barred from obtaining any remedy.

A g r o n o m i e e t E n v i r o n n e m e n t

30 rue de la Station – 68700 ASPACH LE BAS – Phone: 03 89 62 72 30 – Fax: 03 89 62 72 49 e-mail: [email protected] – site web: www.sadef.fr

AGENT Field trial: Evaluation of the efficiency of the product Kaitosol® on potatoes

September 2013

Study n° C 13-10

NL X-00029-13

This document may not be copied without the authorisation of SADEF.

NL X-00029-13 1

Content

I. TEST PROTOCOL ....................................................................................... 2

1. PURPOSE OF THE TEST........................................................................................................................ 2

2. DEVICE TESTI3G A3D TECH3ICAL CHARACTERISTICS .................................................................... 2

II. TEST RESULTS ............................................................................................. 3

1. FLUORIMETRIC MEASUREME3T: PHOTOSY3THETIC EFFICACY ..................................................... 3

A) INITIAL FLUORESCENCE (F0)............................................................................................................................ 3

B) PHOTOSYNTHETIC EFFICIENCY (FV/FM) ........................................................................................................... 3

2. I3TERMEDIATE HARVEST ................................................................................................................... 4

3. FI3AL HARVEST RESULTS: YIELD A3D CALIBRATIO3 ...................................................................... 4

III. TEST CO3CLUSIO3S ................................................................................ 5

NL X-00029-13 2

Study C 13-10

Evaluation of the efficiency of the product Kaitosol® on potatoes

SADEF - Service Expérimentation Rue de la Station – F-68700 ASPACH LE BAS

Phone. 03 89 62 72 30 – Fax 03 89 62 72 49

I. Test protocol

1. Purpose of the testThe objective of this field trial is to evaluate the efficacy of Kaitosol® on the development of potatoes.

2. Device testing and technical characteristics

This trial was made on a potato crop (Solanum tuberosum, variety Josée). The field used is located in Traubach-le-Bas (68) in France.

The physico-chemical analysis of the test plot is presented in Appendix 4. The test consists of two treatments arranged on four replicates. A repetition is a micro-plot of 5 m by 3.5 m (see map in Appendix 1).

Treatment Product 1rst application

(L / ha) Application 2 to 6

(L / ha)

T1 / / /

T2 Kaitosol® 1 2

Table 1 : Trial description.

The potato crop was conducted in a traditional farming way (soil preparation, planting, fertilizers and pesticides applications) by the farmer who owns the land. Six sprays were made all along the test. These applications were made by us and using an equivalent to an application of slurry of 550 L / ha.

Two fluorimetric measurements (photosynthetic activity measurement) were made at two different vegetative stages: golf balls and end of flowering / sizing of tubers. At the end of the flowering, an intermediate harvest was made for each micro-plot, with a global biomass production measurement and a tubers biomass production measurement. The harvest was made manually taking care of minimizing losses. Two kinds of measurements were made: yield and calibration.

The crop calendar is presented in Appendix 2 and the raw datas are presented in Appendix 3. Statistical analyses (variance analyses with a 95% Fischer-LSD comparison/classification confidence interval) were made using the XLSTAT 2010 software.

NL X-00029-13 3

II. Test results 1. Fluorimetric measurement: photosynthetic efficacy

a) Initial fluorescence (F0) The initial fluorescence gives an indication of the condition of the plants: the higher the value, the higher the plant is in trouble.

Treatment Measure n°1 Measure n°2

T 1 737 a 634 a

T 2 779 a 599 b

Threshold αααα 0,363 0,047

Standard deviation 124,0 39,0

CV (%) 16,3 6,3

Table 2: Statistical analyses of initial fluorescence The letters in bold type in the table indicate homogeneous statistical groups. At the “golf ball” vegetative stage (measure n°1), no significant difference was observed between the untreated plants and plants treated with the product. The second measure, made at the end of flowering, shows a significant difference between the control and treated modality. The value of T2 is lower than the control, it means the treated plants are better than the control plants.

b) Photosynthetic efficiency (Fv/Fm) The measurement of the photosynthetic activity (Fv/Fm) shows the global photosynthetic efficiency. Many studies have shown that optimum operation is achieved when the level exceeds 0,800, demonstrating a maximum use of the light energy for the photochemical conversion.

Treatment Measure n°1 Measure n°2

T 1 0,675 a 0,741 a

T 2 0,673 a 0,749 a

Threshold αααα 0,921 0,149

Standard deviation 0,069 0,014

CV (%) 10,2 1,8

Table 3: Statistical analyses of photosynthetic activity

NL X-00029-13 4

The statistical analysis did not highlight any significant difference between the control and T2. The values obtained at the end of trial appear to a slight advantage to the product tested. 2. Intermediate harvest The table below shows the results obtained at the intermediate harvest at the end of the flowering.

Treatment

Aerial parts Tubers

Fresh matter (g) Dry matter (g) % of dry

matter Number of

tubers Fresh

matter (g) Dry matter

(g) % of dry matter

T 1 1171 a 111 a 9,47 a 42,0 a 979 a 134 a 13,8 a

T 2

1268 a 118 a 9,33 a 35,9 a 1106 a 146 a 13,0 a

+ 8,3 % + 6,6 % - 1,5 % - 14,6 % + 12.9 % + 9.6 % - 5,8 %

Threshold

αααα 0,418 0,386 0,769 0,370 0,920 0,843 0,947

Standard deviation

183 14,5 0,43 8,79 291 42,3 1,62

CV (%) 15,0 12,7 4,6 22,6 28,0 30,3 12,0

Table 4: Statistical analyses of intermediate harvest results

Statistically, no significant differences could be demonstrated on the measured parameters. Nevertheless, it appears that production (aerial parts and tubers) of the plants treated with the product are between 6.6 and 12.9% higher than the production of the untreated plants. 3. Final harvest results: yield and calibration Plots were harvested handly a few days before mechanical harvesting of the whole plot. 4 m of the two central rows of each micro-plot were harvested, representing an area of 6.4 m².

Treatment Small size

(< 35 mm) (kg)

Middle size (35 mm to 55

mm) (kg)

Big size (> 55 mm) (kg)

Fresh yield (t / ha)

% DM of the yield

T 1 1,28 7,23 11,8 32,9 21,2

T 2 1,08 6,96 13,4 34,6 18,1

Threshold αααα 0,447 0,816 0,600 0,695 0,340

Standard deviation

0,32 1,49 3,92 5,53 3,91

CV (%) 26,8 21,0 31,2 16,4 19,9

Table 5: Statistical analyses of the harvest results No significant differences could be established between the control and treated modality for all measured parameters. Nevertheless, there is a better yield for the treated plants 1.7 tons / ha more as compared to the control.

NL X-00029-13 5

III. Test conclusions

This test, the purpose of which was to assess the effects of Kaitosol® on potato crop, has given the following findings:

• Regarding the photosynthetic activity, there has been a better general condition of treated plants versus untreated plants, highlighted by an initial fluorescence significantly lower for plants treated with Kaitosol®.

• The intermediate harvest shows no significant difference between the two treatments. However, trends are clearly observed. Production (aerial part and tubers) of plants treated with the product is on average higher (+ 10%) compared to the production of the control.

At the final harvest, this trend is confirmed with a yield gain of 1.7 tons / ha for treated plants.

A calculation of turnover per hectare in two sales scenarios (regarding French market) allows an estimation of the generated gain after applying the product. Modality with the greatest yield is the modality T2 with 34.6 t / ha compared to 32.9 t / ha for the untreated control, corresponding to a difference of 1.7 tons / ha. For direct sales (0.40 € / kg), the gain is about 679 € / ha, and for wholesale volume (0.15 € / kg), the gain is about 255 € / ha.

It would be interesting to know if under more favorable conditions, the potential of the product is more expressed.

NL X-00029-13 6

Appendix

NL X-00029-13 7

APPENDIX 1

Trial plan

5 m T2 B4 T1 B1

T1 B1 T2 B3

T2 B2 T1 B1

21,5 m

T1 B1 T2 B1

3,5 m (5 rows)

0,7 m (1 row)

0,5

m

NL X-00029-13 8

APPENDIX 2

Crop calendar

Days Operations

14-mai 1rst application

23-mai 2nd application

10-juin 1rst fluo measurement

(golf ball vegetative stage)

10-juin 3rd application

21-juin 4th application

05-juil 2nd fluo measurment + intermediate harvest

(end flowering vegetative stage)

08-juil 5th application

19-juil 6th application

23-août Harvest

NL X-00029-13 9

APPENDIX 3

Raw datas

Fluorimetric measures

Measure n°1 Measure n°2

Treatment Replicate F0 Fv/Fm

Treatment Replicate F0 Fv/Fm

T1 B1 661 0,689 T1 B1 580 0,765 T1 B1 608 0,743 T1 B1 648 0,750 T1 B1 995 0,500 T1 B1 664 0,746 T1 B1 721 0,691 T1 B2 629 0,720 T1 B2 1 111* 0,465* T1 B2 639 0,729 T1 B2 649 0,757 T1 B2 637 0,727 T1 B2 668 0,712 T1 B3 632 0,734 T1 B2 986 0,573 T1 B3 597 0,746 T1 B3 610 0,750 T1 B3 650 0,734 T1 B3 740 0,717 T1 B4 608 0,753 T1 B3 603 0,734 T1 B4 617 0,743 T1 B3 624 0,725 T1 B4 703 0,701* T1 B4 764 0,683 T2 B1 623 0,739 T1 B4 734 0,697 T2 B1 542 0,768 T1 B4 823 0,642 T2 B1 586 0,770 T1 B4 874 0,515 T2 B2 594 0,743 T2 B1 687 0,700 T2 B2 661 0,720 T2 B1 816 0,640 T2 B2 507* 0,768 T2 B1 969 0,601 T2 B3 521 0,769 T2 B1 1 021 0,556 T2 B3 611 0,736 T2 B2 1 019 0,576 T2 B3 653 0,742 T2 B2 672 0,747 T2 B4 586 0,739 T2 B2 634 0,749 T2 B4 617 0,757 T2 B2 696 0,692 T2 B4 595 0,740 T2 B3 708 0,721 T2 B3 706 0,705 T2 B3 659 0,729 T2 B3 772 0,715 T2 B4 702 0,731 T2 B4 833 0,606 T2 B4 839 0,587 T2 B4 724 0,707

* Aberrant values removed for statistical analysis.

NL X-00029-13 10

Results of the intermediate harvest

Treatment Replicate Row Aerial parts Tubers

FM (g) DM (g) % DM 3umber FM (g) DM (g) % DM

T1 B1 1 1104 106 9,6 44 1146 152 13,2

T1 B1 2 1506 138 9,2 47 1590 240 15,1

T2 B1 1 1292 114 8,8 45 728 137 18,8

T2 B1 2 1210 112 9,2 29 872 108 12,4

T1 B2 1 1124 110 9,8 49 1102 147 13,4

T1 B2 2 949 91 9,6 32 911 119 13,0

T2 B2 1 1217 111 9,1 44 1129 134 11,9

T2 B2 2 1110 113 10,2 44 1213 165 13,6

T1 B3 1 950 93 9,8 39 905 123 13,6

T1 B3 2 1426 126 8,8 33 1269 164 12,9

T2 B3 1 1353 123 9,1 42 1100 145 13,2

T2 B3 2 1185 108 9,1 28 785 91 11,6

T1 B4 1 1217 115 9,5 24 603 80 13,3

T1 B4 2 1171 109 9,3 27 873 112 12,8

T2 B4 1 1111 113 10,1 49 1122 150 13,4

T2 B4 2 1590 146 9,2 47 1330 171 12,9

Final harvest results

Treatment Replicate Small size (< à 35 mm) (en

kg)

Middle size (35 mm à 55 mm) (en kg)

Big size (> à 55 mm) (en

kg)

% DM

Area (m²) Yield FM

(t / ha)

T1 B 1 1,39 8,03 11,28 19,9 6,4 33,53

T1 B 2 1,66 7,67 9,92 26,0 6,4 31,11

T1 B 3 0,92 5,83 16,69 16,7 6,4 38,11

T1 B 4 1,13 7,39 9,14 22,3 6,4 28,95

T2 B 1 0,77 5,67 12,81 16,7 6,4 31,14

T2 B 2 1,13 5,92 17,47 16,1 6,4 39,61

T2 B 3 1,20 6,94 10,91 20,4 6,4 30,78

T2 B 4 1,22 9,32 12,32 19,2 6,4 36,92

NL X-00029-13 11

APPENDIX 4

Soil analyses of the trial plot

Laboratoire agréé par le Ministère de l'Agriculture français toutes options - Membre du GEMAS

S. Expérimentation

68700 ASPACH-LE-BAS

PARCELLE

Granulométrie sans décarbonatation NFX 31-107

Bouclage à 100% sur la fraction minérale

Limon argileux

Argile *

Limon fin *

Limon grossier *

Sable fin *

Sable grossier *

Indice de battance

Stabilité structuraleBartoli

Capacité de rétention(pF 2.8)

Point de flétrissement(pF 4.2)°

Calcaire total *NF ISO 10693 Matière organique *

NF ISO 14235

Optimum : 19 g/kg

NF ISO 10390pH Eau * Carbone organique *

NF ISO 14235

Conductivité *NF ISO 13878

Azote total *NF ISO 11265

Calcairetotal pH Rapport C/N Taux de

M.O.

Accréditation COFRAC n° 1-0751 Portée disponible sur www.cofrac.fr

L'accréditation de la section laboratoires du COFRACatteste de la compétence du laboratoire SADEF pour les

seuls essais couverts par l'accréditation.

Banque : C.I.A.L. 40, rue de la Sinne - 68100 Mulhouse - RIB n°10037 33281 00018765701 72 - n° identification CEE : FR 29 385 620 240

SARL au capital de 150.000 euros - Code APE 731 Z - RCS Mulhouse385 620 240 B - Siret 385 620 240 00023

SADEF POLE D'ASPACH Rue de la Station - F-68700 Aspach-le-Bas - www.sadef.frTel : +33 (0)3 89 62 72 30 - Fax : +33 (0)3 89 62 72 49 - Email : [email protected]

Rapport d'analyse de terre n° : T-04042-13 (0) - Page 1/2

N° Lab :

Dossier :

N° F.R. :

N° EDIS : SERRE.644.1

WW 399011

LAB13 7360

T-04042-13 (0) Prof. : 25 cm

-

0 %

0 %Cailloux :

Refus tamis :

Masse :

Nom :Commune :Surface : 1 ha

TRAUBACH-LE-HAUTPDT TRAUBACH

Coordonnées gps :

Prélèvement : 24/04/2013

26/4/2013Arrivée Labo :

26/04/2013Début analyse :

Fin analyse :

Edition : 23/05/2013

23/05/2013

0 %

6

-

28.4 g/kg

16.4 g/kg

-

-

19.5 %

37.8 %

34.3 %

4 %

4.4 %

-

-

-

-

SADEF EXPERIMENTATION

ECHANTILLON : PDT TRAUBACH

1.7Sol assez battantCalculé (Rémy Marin-Laflèche)

Accréditation COFRAC n° 1-0751 Portée disponible sur www.cofrac.fr

Composition du complexeargilo-humique

Saturation ducomplexe 100.0 %

CEC Metson à pH 7 *

2+0% 50% 100%NF X 31-130

Ca

K+

Mg+

Na

2+

CEC au pH du solCalculée

+HCEC à

pH 7CEC au

pH du sol

Optimum

20 mg/kg

8 mg/kg

0.8 mg/kg

0.4 mg/kg

0.26 mg/kg

-

Teneur du sol

Fer (Fe) *

Manganèse (Mn) *

Zinc (Zn) *

Cuivre (Cu) *

Bore (B)

Molybdène (Mo)

DTPA - NF X 31-121

DTPA - NF X 31-121

DTPA - NF X 31-121

DTPA - NF X 31-121

Eau chaude - NF X 31-122

Grigg

Fe Mn Zn Cu B

T-04042-13 (0) - Page 2/2Rapport d'analyse de terre n° :

Ce rapport d'essai ne concerne que les objets soumis à l'essai. L'accréditation de la sectionlaboratoires du COFRAC atteste de la compétence du laboratoire SADEF pour les seuls essais

couverts par l'accréditationCe rapport ne doit pas ête reproduit sans l'approbation du laboratoire d'essai. La reproduction de ce

rapport d'essai n'est autorisée que sous sa forme intégrale.(*) : Essais couverts par l'accréditation.Les résultats sont exprimés par rapport à la masse de terre

fine sèche à 2 mm. L'interprétation est hors champ d'accréditation.

88.7 %

1.4 %

9.7 %

0.3 %

0.0 %

118 mé/kg

105 mé/kg

181 mg/kg

22.7 mg/kg

4.8 mg/kg

2.6 mg/kg

0.24 mg/kg

-

Optimum Optimum Optimum Optimum Optimum Optimum Optimum

Teneur du sol Optimum

Phosphore - P O *Joret-Hebert - NF X 31-161

2 5 0.05 g/kg 0.16 g/kg

Phosphore - P O *2 5Olsen - NF ISO 11263

0.026 g/kg 0.08 g/kg

Optimum Optimum Optimum Optimum Optimum Optimum Optimum Potasse - K O *2

NF X 31-1080.07 g/kg ou 1.6 mé/kg 0.17 g/kg

Magnésie - MgO *NF X 31-108

0.23 g/kg ou 11.5 mé/kg 0.1 g/kg

NF X 31-108

Chaux - CaO * 3.1 g/kg ou 110.6 mé/kg 2.93 g/kg

Sodium - Na O *NF X 31-108

2 0.01 g/kg ou 0.3 mé/kg -

( 0.1 )K O / MgO (K/Mg)2 0.3 1 à 2 Joret H DyerP O

K O2 MgO CaO K O2

2 5 MgO (0,4 à 0,8)

Sylvie LHOTEResponsable Dépt TERRE

Protocol for FPA Experiment Title: The Efficacy of KaitoSol®Plant for Pechay (var Brassica)

I. Introduction Pechay belongs to the green leafy vegetable and has been used in Asian cooking for centuries. The leaves are delicious and crispy, and are excellent sources of Vitamin A, folic acid and essential elements such calcium and potassium, and fiber. Pechay is a cash crop and hence, for the farmers to get their returns at a shorter time, the need to employ fertilization should be looked into. KaitoSol®Plant is a natural product developed from natural shrimp base and plant extracts with no negative effect on the environment. It is used as a growth enhancer through the powerful action of its ingredients at the nanoscale. The following are the functions of KaitoSol®Plant: • Increases the yield and quality of productivity by improving the health

and growth of plants. Specifically, it: • Increases branching and increase size of leaves • Increases the ability to absorb more nutrients and micronutrients • Makes the roots of the plants to grow deeper • Helps damaged soil and encourages growth of useful microbes

II. Objective

To evaluate the effectiveness of KaitoSol®Plant, a natural product from shrimp-based and plant extract in promoting higher growth rates and yields of pechay.

III. Researcher:

VERONICA P. MIGO BIOTECH, UP Los Baños College, Laguna, University of Philippines FPA Accreditation No. 143

IV. Materials and Methods

a. Site description

The field efficacy trial will be conducted in San Ildefonso, Bulacan. The area is flat, has adequate irrigation facilities, accessible, has the same previous cropping, fertilizer and cultural treatment history. The area was planted previously with ampalaya. The soil type is clay loam with a pH of 6.8, 0.58 % of organic matter, available P

and K of 96 ppm (Olsen P) and 0.76 cmol (+) kg soil respectively (based on soil chemical analysis). b. Description of product

KaitoSol®Plant is a natural product developed from natural shrimp base and plant extracts with no negative effect on the environment. It is used as a growth enhancer through the powerful action of its ingredients at the nanoscale. It increases the yield and quality of productivity by improving the health and growth of plants. Specifically, it: • Increases branching and increase size of leaves • Increases the ability to absorb more nutrients and micronutrients • Makes the roots of the plants to grow deeper • Helps damaged soil and encourages growth of useful microbes

c. Crop species and variety

Pechay seeds of the variety Pavo or Pavito will be used.

d. Trial Conditions and Experimental Design The experimental design is RCB with 3 replications. The treatments are as follows:

1- Control (no fertilizer) 2- Recommended Rate (RR) of NPK (commercial fertilizer) 3- 0.5 RR of NPK 4- 0.5 RR of NPK + RR of KaitoSol®Plant 5- RR of KaitoSol®Plant 6- RR of NPK + RR of KaitoSol®Plant

e. Method and time of application of the product The seeds will be wrapped with clean cloth (as in Ragdoll method of germinating the seeds), will be dipped in the solution of KaitoSol®Plant overnight. On the following day in the morning, the treated seeds will be air-dried and sown into the potting media in the seedling tray. The seedlings will be raised in the seedling trays for 10-14 days, after which they will be transplanted in the field at one plant per square meter for yield assessment. The 15 m2 plots will be prepared for each replication. The treatments will be replicated 3 times.

f. Recommended cultural practices Land Preparation Thorough land preparation and construction of beds will be done. The field will be plowed and harrowed. A 30-cm high beds will be constructed using a plow. The distance between centers of adjacent furrows will be 150 cm with a 30 cm bed per plot.

Fertilizer Application The recommended rate of commercial fertilizer application for pechay is 55-75 kg N/ha, 40-80 kg P/ha and 80-100 kg K/ha basal application. Two weeks after planting, side dressing of nitrogen is again applied. The recommended fertilizer rate of KaitoSol®Plant is at the ratio 1:100 -(10mL KaitoSol®Plant + 1 Liter water) applied as mist spray to any reachable parts of the plants.

Irrigation and Drainage A furrow irrigation method will be employed every 5 days or whenever necessary. Harvesting and handling The harvesting is expected to start 45 days after transplanting.

V. Parameters to be gathered at harvest

To evaluate the efficacy of KaitoSol®Plant, the following data will be collected: Yield (marketable clusters)

The marketable size of pechay clusters will be picked up and the total yield for every picking schedule will be obtained. Yield will be expressed in terms of kg/ha.

VI. Results and Discussion:

Table 1 and Figure 1 shows that the application of KaitoSol®Plant with full rate of RRC (T6) gave the highest average weight per plant corresponding to 131 grams. Furthermore, there is an increase of 31.7% in average weight per plant when KaitoSol®Plant was combined with full rate of conventional fertilizer, RRC (T6 versus T2). The positive combined effects of KaitoSol®Plant were observed when it is added with full rate of RRC but not with half rate of RRC. However, the application of half rate RRC with

KaitoSol®Plant is better than the control. KaitoSol®Plant alone (T5) performs very well than the conventional fertilizer at both half and full rate dose.

In terms of yield, the data in Table 2 and Figure 2 showed that the highest yield of 26.2 tons per hectare was observed in T6 (KaitoSol®Plant +RRC). Similarly, there is an increase of about 32% in tons per hectare when KaitoSol®Plant was combined with full rate of RRC (T6 versus T2). The positive effects of KaitoSol®Plant were observed when it is added with full rate of RRC (T6) but not with half rate of RRC (4). The application of KaitoSol®Plant alone resulted to an increase in yield of 30.6% compared to full rate of RRC and 28.3% compared to half rate of RRC. The plants with KaitoSol®Plant treatments have more vigor and the leaves are deeply rooted. There was no significant incidence of pests and diseases and water stress throughout the growing period of the plants since there was regular irrigation at the experimental site. The following are the functions of KaitoSol®Plant in terms of increasing yield and quality of crop productivity: 1. Increases the immunity and strengthens the plants defence system by

inducing chitinase enzymes and phytoalexin; 2. Improves plant health and growth; 3. Makes the roots of the plants grow deeper and wider; 4. Increases branching and increases size of leaves; 5. Increases the ability to absorb more nutrients and micronutrients; and 6. Makes the infected and damaged plants recover faster

VII. Conclusions:

1. The supplementation of KaitoSol®Plant as an organic fertilizer was

observed in treatments where KaitoSol®Plant was combined with full rate of RRC but not with half rate. However, the application of half rate RRC with KaitoSol®Plant is better than the control. These are observed in both the average weight per plant and yield in tons per hectare.

2. KaitoSol®Plant when applied alone performs than those in treatments with RRC in both half and full rate doses. Therefore KaitoSol®Plant can stand in itself as the lone organic fertilizer without the addition of conventional inorganic fertilizer.

VIII. References:

1. Dufault, R.J. 1985. Relationship among nitrogen, phosphorus, and potassium fertility regimes on celery transplant growth. Hort Science 20, 1104-1106.

2. Kratky, B.A. and Mishima, H.Y. 1981. Lettuce seedling and yield

response to pre-plant and foliar fertilization during transplant production. Journal of the American Society of Horticultural Science 106, 3-7.

3. Masson, J.,Tremblay, N. and Gosselin, A. 1991. Nitrogen fertilization

and HPS supplementary lighting influence vegetable transplant production. Journal of the American Society of Horticultural Science 116, 594-598.

Table 1. Average weight of pechay (in grams) as affected by KaitoSol®Plant.

Treatment Replication Mean* 1 2 3

T1- Control 34 35 35 34.67 F T2 – RRCF 90 89 89.5 89.5 D T3 – 0.5 RRCF 92 92.3 93 92.43 C T4 – 0.5 RRCF + RR Acc 70 68 69 69.0 E T5 – RR Acc 128 128.7 130 128.9 B T6 – RRCF + RR Acc 130 131 132 131 A RRCF – Recommended Rate of Chemical Fertilizer RR Acc - Recommended Rate of KaitoSol®Plant *Means followed by the same letter are not significantly different as 5% alpha. Table 2. Yield of pechay (in tons per hectare) as affected by KaitoSol®Plant.

Treatment Replication Mean* 1 2 3

T1- Control 6.8 7 7 6.93 F T2 – RRCF 18 17.8 17.9 17.9 D T3 – 0.5 RRCF 18.4 18.46 18.6 18.49 C T4 – 0.5 RRCF + RR Acc 14 13.6 13.8 13.8 E T5 – RR Acc 25.6 25.74 26 25.78 B T6 – RRCF + RR Acc 26 26.2 26.4 26.2 A RRCF – Recommended Rate of Chemical Fertilizer RR Acc - Recommended Rate of KaitoSol®Plant *Means followed by the same letter are not significantly different as 5% alpha.

0

20

40

60

80

100

120

140

T1- Control T2 – RRCF T3 – 0.5 RRCF T4 – 0.5 RRCF + RR Acc

T5 – RR Acc T6 – RRCF + RR Acc

Average weight of pechay (in grams) as affected by KaitoSol®Plant

Mean*

Aver

age

wei

ght o

f pec

hay

(in g

ram

s)

0

5

10

15

20

25

30

T1- Control T2 – RRCF T3 – 0.5 RRCF T4 – 0.5 RRCF + RR Acc

T5 – RR Acc T6 – RRCF + RR Acc

Yield of pechay (in tons per hectare) as affected by KaitoSol®Plant

Mean*

Yiel

d of

pec

hay

(in to

ns p

er h

ecta

re)

Photographs of the standing crop at different treatments:

Photographs of the harvested pechay plants at different treatments:

Testing of the Kaitosol

IN KG.THOM, CAMBODIA

30Kg/10mx10m Control

19Kg/1 Omx1 Om

Advanced Green Nanotechnologies Sdn. Bhd.No 2,Jalan Industri USJ 1/11, 47600 Subang Jaya, Selangor, Malaysia.Tel: +603-8023 5425 / Fax:8023 5426 Email : [email protected]

Manufactured By:

Distributed By:

Email : inquiry@agent

Distributed By: Global Green TechK203, Purva Highlands,Mallasandra Village, Uttarahalli, Hobli,Off Kanakapura Road, Bengaluru 560068, Karnataka, IndiaTel: +91-9989755008 / [email protected]

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