biosope – macro-particles 1- scientific objectives

BIOSOPE – macro-particles

1- Scientific objectives

We are proposing two projects:

A- Estimation of the stock of particulate matter (PM) > 100 µm in the studied water column and the relationship between PM’s spatial pattern and the bio-physical data.

B- Estimation of the distribution and of the diversity of meso- and macrozooplankton. Relation between their size-dependent distribution and ecosystem state. NET SAMPLING.

Methods –ZOOSCAN and OPC for treatment of zooplankton

OPC

Triple net ZOOSCAN

1) Pour and distributethe sample in the cell

2) Digitize and analyze thepicture in one mouse click

3) Collect the sampleback without lost

or degradation

Easy 3 steps dataacquisition

<15 min

Image digitization

200 µm

20% of the whole image

Each picture is 17,500 x 7,000 pixels and contains hundreds of animals

Image analysis1 mm

010

0000

2500

00

total

y

janv. mai juil. sept. nov. janv.

24

68

AcartiaSp

y


46

810

14

CalyptopisSp

y


0.0

0.5

1.0

1.5

CavoliniaInflexa

y


510

1520

25

CentropagesSp

y


02

46

810

Chaetognathes

y


510

2030

CladoceransOthers

y


510

1520

ClausoParacalanus

y


3040

5060

70

Copepodit

y


0.5

1.0

1.5

Coryceidae

y


0.0

1.0

2.0

CreseisSp

y


0.2

0.6

1.0

1.4

EggOthers

y


12

34

5

EuterpinaSp

y


0.0

0.2

0.4

FishEgg

y


0.00

0.10

0.20

0.30

FishLarvay


05

1015

FritillariaSp

y


12

34

5

Hydrozoa

y


24

68

10

MolluskEggs

y


02

46

8

MolluskOthers

y


0.5

1.5

2.5

3.5

Mysis

y


05

1015

OikopleuraSp

y


1020

3040

OithonaSp

y


020

4060

PeniliaSp

y


02

46

8

Pluteus

y


2040

6080

reject

y


12

34

5

Salp

y


02

46

812

SiphonoCali

y


0.0

1.0

2.0

SiphonoPhyso

y


46

810

12

TemoraSp

y


0.5

1.5

2.5

3.5

Zoe

y


Size distribution (or temporal evolution) of the different faunistical groups determined by the ZOOSCAN

3- Sampling needs

Dig-UVP will be mounted on the CTD (hopefully)we need a dry lab place for a large desktop computer, printer and a laptop computer

Zooplankton net sampling – according to the schedulewe need a storage place for the fixed samplespreservative = buffered formaldehyde

Zooscan/OPC – the treatments will be carried out in France

Results50

6070

8090

Methods accuracies and timings (8 external samples)

accu

racy

(%)

5060

7080

90

lda qda mda fda knn lvq tree rpar bagg db.l db.k rfor svm nnet dvf

lda qda mda fda knn lvq tree rpar bagg db.l db.k rfor svm nnet dvf

time

to p

redi

ct 1

0,00

0 ite

ms

(sec

)

05

1015

2025

05

1015

2025

• 15 methods tested and compared

• Recognition accuracies higher than 80% are difficult to obtain

• Our new method (dvf, discriminant vector forest) is both efficient and reasonably fast

biosope – macro-particles 1- scientific objectives

Documents