bioplastic from waste 1confidential ryan smith, cto [email protected]
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Micromidas converts biological waste into biodegradable plastic
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Micromidas converts biological waste into biodegradable plastic
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Jackson
Annually, 150 million tons of plastic is thrown “away”
PHA (Poly-hydroxyalkanoates)a family of linear aliphatic polyesters
In the environment PHAs are biodegraded by many species of bacteria.
PHA
Plastic requires 8% of the world’s oil
PHA granules in bacteria
Poly-hydroxyalkanoates (PHAs) are naturally synthesized biological polyesters. They serve as a carbon energy storage compound for bacteria and are stored as intracellular granules.
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SugarOil
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Feedstock LandscapeNew is as bad as the Old
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New is as bad as the OldFeedstock Landscape
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Wastewater Solids
Sludge is a by-product of treatment
Solids Disposal is 40% of treatment cost8,200,000 MT/year in US
Treatment Cost: $100-500/MT
Sludge capacity limited
Increasingly regulated
Actively seeking new treatment options
Inflow (100%) Primary Solids
Primary Effluent
Biosolids (43%)
Bio Gas (21%)
CO2 (33%)
Secondary Effluent (3%)
Activated Sludge
Digestion
2o Clarifier
1o Clarifier
Aeration Pond
Relative Carbon Flow through Wastewater treatment plant (WWTP)
Inflow (100%) Primary Solids
Primary Effluent
Residual Solids (32%)Lignin, Pulped Cellulose,Inorganic Salts, Silicates
Bio Gas (3%)
Soluble Carbon
CO2 (18%)
PHA (9%)
Cells (3%)
CO2 (33%)
Secondary Effluent (3%)
Activated Sludge
Micromidas
Acid Phase Digestion
2o Clarifier
1o Clarifier
Aeration Pond
Relative Carbon Flow through Micromidas integrated WWTP
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Technology and Tools
Techalive.mtu.eduHiwtc.com
Wastewater Style ProcessingLow Cost
HIGH VOLUME
Existing Operators
Same Equipment
Infrastructure in place
Ve r y Ro b u s t
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2010.igem.org/Team:INSA-Lyon/Project/Stage2/Theory
Selected bioprospecting
Source MicrobesSoil
Aerobic TreatmentPond Water
Evolve
Basic SelectionGenetic
100 ml growth100 ml production
Resist contamination
EvaluationProcess
Metabolic
Ralstonia Eutropha H16
Pseudomonas putida KT 2440
Bacillus cereus
Productive Strains
Microbe Library
DEA-003
Micromidas was formed in 2008 and is currently working with several wastewater treatment facilities across the world to help address problems of costly bio-solids removal.
Micromidas developed a fermentation technology that partitions the organic nutrients from waste and then utilizes a robust set of organisms to store the energy from those nutrients as intracellular bio-plastic.
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