Biomedical Sciences & Pathobiology Faculty S. Ansar Ahmed, DVM, PhD

Professor, Immunology Associate Dean, Research and Graduate Studies Director, Summer Veterinary Scholars Research Program Professor, Faculty of Health Sciences Email: ansrahmd@vt.edu

Education 1985 – 1987 Leukemia Foundation of America Fellow, The University of Texas Health Science

Center at San Antonio 1985- PhD

School of Veterinary Studies The Murdoch University, Australia

1978- DVM (BVSc) University of Agricultural Sciences

Administrative Experience:

Director, Center for Molecular Medicine and Infectious Diseases (now renamed as the Center for

One Health Research). (2002-2008) This is a college research center that focuses on infectious

diseases and immunology research, and development of vaccines using animal models. The

renamed center is a collaboration with the Virginia College of Osteopathic of Medicine to address

the above areas using one health approach. Interim Head, Department of Biomedical Sciences and Pathobiology: (2007-2008)

Head, Department of Biomedical Sciences and Pathobiology, VMCVM (2008-2017)

Director, Summer Veterinary Scholars Research Program (2007 to date). This is a college-wide

program, funded by NIH-T35, Boehringer-Ingelheim, and the college to train DVM students from

a national pool of applicants for research in biomedical research.

Head, Biomedical Veterinary Sciences and Graduate Program VMCVM, (2017- to date):

Associate Dean, Research and Graduate Studies, VMCVM (2017- to date)

Chair, Graduate Affairs Committee, VMCVM (2017-to date)

Chair, VMCVM Research Committee (2017-to date)

Testing Facility Management, Good Laboratory Practice Program at the College of Veterinary

Medicine (2017- to date)

Research Interests

The primary research interest in Dr. S. Ansar Ahmed’s laboratory is to investigate why the immune system launches a misdirected attack on self-tissues leading to devastating chronic autoimmune diseases, which afflict millions and costs billions in health care costs. Lupus is a prototypical autoimmune disease that also afflicts domesticated species, including dogs and cats. We employ various in vivo wildtype, gene knockouts, and transgenic lupus murine models to delineate pathogenic autoimmune mechanisms. Thus far, there are no effective cures for lupus. Therefore, our long-term goal is to develop innovative therapies for autoimmune conditions since current therapies (immunosuppressive drugs) have significant side effects.

Our laboratory is actively engaged in addressing the following two main central questions in autoimmune diseases.

• First, why are autoimmune disorders occur preferentially in females? Our laboratory is nationally/internationally recognized in this area. We are actively identifying the underlying reasons for the female predominance of lupus and the contribution of estrogens (including synthetic and environmental) towards the risk for developing autoimmune diseases. In particular, we are investigating hormonal factors (e.g., natural and environmental estrogens) alter signaling pathways, transcription factors and gene activation events involved in the induction of inflammatory cytokines in murine models of chronic inflammatory and autoimmune diseases.

• Second, what is the role of epigenetics in lupus inflammation? We are investigating how epigenetics (microRNA, altered methylation, and acetylation) influences lupus and inflammation. microRNAs are naturally occurring small RNAs that regulate the immune system in healthy and autoimmune individuals. We were one of the first laboratory to identify a signature expression profile of microRNAs in mice exposed to estrogen, and between sexes in lupus-prone mice. Importantly, select microRNAs have been shown to directly down-regulate inflammatory proteins. We are utilizing conditional miRNA knockout mice in lupus background to understand how they regulate autoimmune conditions. The identification of signature microRNA expression profile in autoimmune states is of prognostic, diagnostic, and therapeutic importance.

Dr. Ansar Ahmed also directs the DVM summer program that is funded by NIH- T35, Boehringer-Ingelheim and the college to provide research experience opportunities for DVM students. More details are available at http://www.vetmed.vt.edu/research/svsrp/index.asp

Professional Experience

2016 – Present Associate Dean, Research and Graduate Studies VA-MD College of Veterinary Medicine, Virginia Tech

2008 – 2016. Department Head

Department of Biomedical Sciences and Pathobiology VA-MD College of Veterinary Medicine, Virginia Tech

2001 – Present Professor of Immunology Department of Biomedical Sciences and Pathobiology VA-MD College of Veterinary Medicine, Virginia Tech

2014 – Present VT Faculty of Health Sciences, Translational Biology, Medicine, Biology and Health Program

Professional Memberships

American Association of Immunologists International Cytokine Society OSSD Phi Zeta Kappa Honor Society Member of Grant Review Panels Served on 26 NIH study sections, and 12 National Foundation grant review panels, and several national-level research policy panels

My Bibliography: http://www.ncbi.nlm.nih.gov/sites/myncbi/1xg0wt73uBLAO/bibliography/48807843/public/?sort=date &dir ection=ascending

Recent key Publications:

• Dai R, Edwards MR, Heid B, Ansar Ahmed, S. 17beta-Estradiol and 17alpha-Ethinyl Estradiol Exhibit Immunologic and Epigenetic Regulatory Effects in NZB/WF1 Female Mice. Endocrinology. 2019;160(1):101-18. PubMed PMID: 30418530. PMCID: PMC6305969.

• Wang Z, Heid B, Dai R*, Ansar Ahmed, S*. Similar dysregulation of lupus-associated miRNAs in peripheral blood mononuclear cells and splenic lymphocytes in MRL/lpr mice. Lupus Sci Med.2018;5(1):e000290. PMID: 30515297; PMCID: PMC6241985. * Corresponding author.

• Thomas Brickler, Amanda Hazy, Fernanda Guilhaume Correa, Rujuan Dai, Elizabeth Kowalski, Ross Dickerman, Jiang Chen, Xia Wang, Paul Morton, Abby Whittington, Ansar Ahmed, S, and Michelle Theus: Angiopoietin/Tie2 axis regulates the age-at-injury cerebrovascular response to traumatic brain injury Journal of Neuroscience 2018; 38 (45): 9618-9634 PMID: 30242049 PMCID: PMC6222064, DOI: 10.1523/JNEUROSCI.0914-18.2018

• Edwards MR, Dai R, Heid B, Cecere TE, Khan D, Mu Q, Cowan C, Luo XM, Ansar Ahmed S. Commercial rodent diets differentially regulate autoimmune glomerulonephritis, epigenetics and microbiota in MRL/lpr mice. International Immunology 2017 Jun 1;29(6):263-276. doi: 10.1093/intimm/dxx033. PMID: 28637300. PMCID: PMC5890898.

• Dai R*, Lu R, Ansar Ahmed, S*. The Upregulation of Genomic Imprinted DLK1-Dio3 miRNAs in Murine Lupus Is Associated with Global DNA Hypomethylation. PLoS One 2016;11(4):e0153509. PMID: 27070142. PMCID: PMC4829153. * Corresponding author.

Contributions to Science

1. Sex Differences and Autoimmune Diseases: In my early career, I was intrigued by the observation of marked sex differences in autoimmune diseases such as lupus and autoimmune thyroiditis. One aspect that we investigated was the role of sex hormones in these autoimmune diseases, an area that had not received much attention at that time. My initial work with my mentors resulted in publications that

received high citations and stimulated interest in the field resulting in many laboratories examining the effects of sex hormones in various other autoimmune diseases, inflammatory and physiological conditions.

• Ansar Ahmed S, Penhale WJ, Talal N. Sex hormones, immune responses and autoimmune diseases: Mechanisms of sex hormone action. American Journal of Pathology. 1985; 121(3):531-51. Review. PMID: 3907369. PMCID: PMC1887926. (Cited times: 1008 in Google scholar; 651 - Web of Science)

• Ansar Ahmed S, Talal N: Sex hormones and the immune system - Animal data. In: Pregnancy and the rheumatic diseases. Edited by: Parke, AL. Bailliere’s Clinical Rheumatology. 1990; 4(1): 13-31. PMID: 2282659. (Cited times: 140-Google Scholar; 118 in Web of Science,)

• Ansar Ahmed S, Dauphinee MJ, Talal N. Effect of short-term administration of sex hormones on normal and autoimmune mice. Journal of Immunology. 1985; 134:204-210. PMID: 3964814 (Cited times: 210 in Google Scholar; 164 - Web of Science;)

• Lucas JA, Ansar Ahmed S, Casey ML, MacDonald PC: Prevention of autoantibody formation and prolonged survival in New-Zealand Black New-Zealand white F1-mice fed dehydroisoandrosterone Journal of Clinical Investigation. 1985; 75 (6): 2091-2093. PMID: 3159756. PMCID: PMC425571. (Cited times: 166 in Google Scholar; 142 - Web of Science;)

2. The regulation of the immune system by estrogens and endocrine disruptors (synthetic estrogens): Upon joining the faculty at Virginia Tech, I recognized that there were major gaps in the understanding of how estrogens affect the normal immune system, especially during prenatal and young adult life. With NIH RO1 support to investigate the immunological consequences during prenatal life and additional RO1 support to investigate how estrogens affect inflammatory cytokine signaling, we made notable strides in this field. Our group was in the forefront in the field to report immunomodulatory effects of estrogen and endocrine disruptors, and its potential influence on autoimmune diseases. The following four publications document our experience in the field.

• Dai, R., Phillips, R., Karpuzoglu, E., R., Ansar Ahmed S. Despite inhibition of nuclear localization of NF-kB p65, C-Rel., Rel-B, estrogen upregulates NF-kB p50/p50: Role of Bcl3. Journal of Immunology. 2007;179(3):1776-83. PMID: 17641044. (Cited times: - 67- Google Scholar; 44-Web of Science).

• Karpuzoglu E, Phillips RA, Dai R, Graniello C, Gogal RM Jr, Ansar Ahmed S. Signal transducer and activation of transcription (STAT) 4beta, a shorter isoform of IL-12-induced STAT-4, is preferentially activated by estrogen. Endocrinology. 2008; 150(3):1310-20 2009. PMID: 18988675. PMCID: PMC2654738.

• Khan D, Dai R, Karpuzoglu E, Ansar Ahmed S. Estrogen increases, whereas IL-27 and IFN-gamma decrease, splenocyte IL-17 production in WT mice. European Journal of Immunology. 2010; 40(9):2549-56. PMID:20623549. PMCID: PMC3097107.

• Calemine, JB., Zelanka, J., Karpuzoglu-Sahin, E., Lengi,A., Ward,D., Ansar Ahmed S. Immunological Changes induced in Geriatric Mice by Estrogenic Endocrine Disruptors

(Diethylstilbestrol, Alpha-Zearalanol, and Genistein): Effects on IFN- mRNA and protein. Toxicology. 2003;194(1-2): 115-128. PMID:14636701.

3. Estrogen regulation of microRNAs: One important mechanism by which estrogens regulate the immune system is through alterations of epigenetics (miRNA and methylation). Estrogens accelerate the aberrant pattern of miRNA expression as evidenced by an earlier expression of lupus-associated miRNA upon estrogen treatment of relatively resistant male-lupus prone mice.

• Dai R, Phillips RA, Zhang Y, Khan D, Crasta O, Ansar Ahmed S. Suppression of LPS-induced IFN and nitric oxide in splenic lymphocytes by select estrogen-regulated miRNA: A novel mechanism of immune modulation. Blood 1;112(12):4591-7. (2008) PMID: 18791161. PMCID: PMC2597130. (Considered as a highly cited publication- Cited in: 140- Web of Science; 190- Google scholar).

• Dai R, McReynolds S, LeRoith T, Heid B, Liang Z, and Ansar Ahmed, S. Sex differences in the expression of lupus-associated miRNAs in splenocytes from lupus-prone NZB/WF1 mice. BMC-Biology of Sex Differences. 2013; 4(1):19. PMID: 24175965. PMCID: PMC3843556.

• Dai, R and Ansar Ahmed S. Sexual dimorphism of miRNA expression: a new perspective in understanding sex bias of autoimmune diseases. Therapeutics and Clinical Risk Management. 2014; 10: 151-163, (Invited Review). eCollection 2014. PMID: 24623979. PMCID: PMC3949753.

• Khan, D., Dai, R., Ansar Ahmed S. Sex differences and Estrogen regulation of miRNAs in lupus, a prototypical Autoimmune Disease. Cellular Immunology, 2015 Apr; 294(2):70-9. PMID: 25619140.

4. MicroRNAs and Autoimmune diseases: Our initial findings on miRNA regulation by estrogens led us to the identification of unique expression of miRNA patterns in lupus-prone mice. These miRNAs appear before the expression of lupus parameters and correlate with the course of the disease.

• Dai, R., and Ansar Ahmed S. MicroRNA, a new paradigm for understanding immunoregulation, inflammation, and autoimmune diseases. Translational Research. 2011; 157(4):163-79. PMID:21420027. PMCID: PMC3072681. (Considered as a highly cited publication- Cited in: 355-Google Scholar; 238 citations- Web of Science;)

• Dai R*, Zhang Y, Khan D, Heid B, Caudell D, Crasta O and Ansar Ahmed S*. Identification of a Common Lupus Disease-Associated microRNA Expression Pattern in Three Different Murine Models of Lupus. PLoS One 2010, 5 (12):e14302. PMID: 21170274; PMCID: PMC3000827. * Corresponding author.

• Dai, R., and Ansar Ahmed, S. microRNAs, important epigenetic regulators of immunity and autoimmunity. Translating MicroRNAs to the Clinic. Chapter 11. 1st Edition. (Editors): Jeffrey Laurence; Hardcover ISBN: 9780128005538; eBook ISBN: 9780128005545; Academic Press; 29th September 2016.

• Edwards M, Dai R, Ansar Ahmed, S. Our environment shapes us: The importance of environment and sex differences in regulation of autoantibody production. Frontiers in Immunology. 2018; 9:478. eCollection 2018. PMID: 29662485 PMCID: PMC5890161.

5. Microbiota and Autoimmune: In collaboration with our departmental colleague, Dr. Xin Luo, we are actively investigating the role of microbiota in lupus. Lupus-prone mice have unique microbial dysbiosis and exhibit leaky-gut that has a potential role in regulating autoimmune diseases. We also found that diet contributes to altered microbiota.

• Xin M. Luo, Michael R. Edwards, Christopher M. Reilly, Qinghui Mu and Ansar Ahmed S. Diet and Microbes in the Pathogenesis of Lupus. In: Lupus , 2017 http://dx.doi.org/10.5772/68110 http://www.intechopen.com/books/lupus.

• Mu, Q., Zhang, H., Liao, X., Lin, K., Liu,H., Edwards, M., Ansar Ahmed, S., Yuan, R., Li, L., Cecere, T., Branson, D., Kirby, JL., Goswami, P., Leeth, C., Read, KA., Oestrich, KJ., Luo, XM. Control of lupus nephritis by changes in gut microbiota. Microbiome. 2017; 5 (1) 73. PMID: 28697806. PMCID: PMC5505136.

• Luo XM, Edwards MR, Mu Q, Yu Y, Vieson MD, Reilly CM, Ansar Ahmed S., Bankole AA. Gut microbiota in human systemic lupus erythematosus and a mouse model of lupus. Applied Environmental Microbiology. 2018; 84(4):e02288-17. PMID: 29196292. PMCID: PMC5795066.

• Mu Q, Tavella, VJ, Kirby JL, Cecere T, Chung M, Lee, J, Li, Ansar Ahmed, S, Eden K, Allen, IC, Reilly CM, Luo XM. Antibiotics ameliorate lupus-like symptoms in mice. Scientific Reports, 2017; 7(1): 13675. PMID: 29057975. PMCID: PMC5651817.

Citation Honors (Source” Google Scholar and ISI Web of Science

Google Scholar Citation indices

All

Citations 7339 h-index 41 i10-index 79

Cited in ~ 1000 (Google Scholar) and over 500 (Web of Science) other Publications:

Cited in 1326 publications by Google Scholar: Cited in 789 publications- Web of Science. Ansar Ahmed S, Gogal, R.M. Jr., Walsh, J.E A new rapid and simple non-radioactive assay to monitor and determine the proliferation of

lymphocytes: an alternative to [3H] thymidine incorporation assay Journal of Immunological Methods 170: 211-224, 1994. http://www.vtnews.vt.edu/articles/2009/08/2009-600.html

Cited in 1008 publications- Google Scholar: Cited in 529 other publications –Web of Science: Ansar Ahmed S, Penhale WJ, Talal N: Sex hormones, immune responses, and autoimmune diseases. Mechanisms of sex hormone action. American Journal of Pathology 121:5 31-559,1985.

Cited in over 150 -350 other Publications:

Cited in 355 publications (Google Scholar): Cited in 122 publications (Web of Science): Dai, R. Ansar Ahmed S, MicroRNA, a new paradigm for understanding immunoregulation, inflammation, and autoimmune diseases Translational Research 157 (4): 163-179, 2011 (ISI Web of Knowledge rates this as “Highly Cited Paper”)

Cited in 263 publications (Google Scholar): Cited in 114 publications (Web of Science). Ansar Ahmed S, The immune system as a potential target for environmental estrogens (endocrine disrupters): a new emerging field Toxicology. 150: 191-206, 2000

Cited in 234 publications (Google Scholar): Cited in 107 publications (Web of Science): Ansar Ahmed S, Hissong BD, Verthelyi D Gender and risk of autoimmune diseases: possible role of estrogenic compounds. Environmental Health Perspectives 107: 681-686, 1999

Cited in 208 publications (Google Scholar): Cited in 139 publications (Web of Science) Ansar Ahmed S, Dauphinee MJ, Talal, N.:

Effects of short-term administration of sex hormones on normal and autoimmune mice. Journal of Immunology 134: 204-210,1985

Cited in 190 publications (Google Scholar): Cited in 140 publications (Web of Science) Dai, R., Phillips, Y., Zhang Y., Khan, D., Crasta, O, Ansar Ahmed, S.: Suppression of LPS-induced Interferon-γ and nitric oxide in splenic lymphocytes by select estrogen-regulated microRNAs: a novel mechanism of immune modulation Blood 112: 4591-4597 Impact factor: 10.896

Cited in 171 publications (Google Scholar): Cited in 134 publications (Web of Science): Lucas JA, Ansar Ahmed S, Casey ML, MacDonald PC: Prevention of autoantibody formation and prolonged survival in New Zealand black/New Zealand white F1 mice fed dehydroisoandrosterone. Journal Of Clinical Investigation 75 (6): 2091-2093 1985

Cited in 152 publications (Google Scholar): Cited in publications (Web of Science): Dai R., Zhang Y., Khan, D, Heid, B, Caudell, D., O Crasta,, Ansar Ahmed S, Identification of a common lupus disease-associated microRNA expression pattern in three different murine models of lupus PLoS One 5 (12): e14302, 2010

Training of Graduate students:

As a Major Advisor:

1) Name of Student: Dr. D.I. Verthelyi, Ph.D. (Immunology), VMRCVM, Dept. Pathobiology Role & Period: Major Advisor, and Chair, Graduate Advisory Committee,1990-1996

Dr. Verthelyi holds a medical degree (MD). (Completed-March, 1996)

Dissertation Title: “Effects of estrogen on the B cell functions of normal mice”.

2) Name of Student: Dr. Zhi-Jun Yin, MS. (Immunology), Dept . of Biomedical Sciences and Pathobiology, VMRCVM,

Role & Period: Major Co-Advisor, (Dr. Sriranganathan, Major advisor) Graduate Advisory Committee,1994-1997 Dr. Yin holds a medical degree (MD). (Completed-July, 1997)

Dissertation Title: “Effect of long-term exposure of normal C57BL/6J inbred mice to 17-

estradiol on gene expression in lymphocytes: mRNA analysis of lymphokines and bcl-2/fas”.

3) Name of Student: Ms. Kelcey Manae Becker MS, VMRCVM, Virginia Tech

Role & Period: Major Advisor, and Chair, Graduate Advisory Committee, 1997-99 (Completed-August,30, 1999)

Dissertation Title: “Effects of short-term exposure to octylphenol and genistein on the immune system of C57BL/6 and (NZB X NZW)F1mice”

4) Name of Student: Ms. Ebru Karpuzoglu Sahin, Ph.D. (Molecular Immunology), Student, VMRCVM, Virginia Tech

Role & Period: Major Advisor, and Chair, Graduate Advisory Committee1999-2005 (Completed-April 7, 2005)

Dissertation Title: “Molecular Analysis of Estrogen-induced alterations in caspases

involved in apoptosis of T and B c ells: role of interferon- ”

5) Name of Student: Role & Period:

Dissertation Title:

Jillian Calamine, M.S. VMRCVM, Virginia Tech . Major Advisor, and Chair, Graduate Advisory Committee, 2001-2004) “Immunological Effects of adult and prenatal exposure to diethylstilbestrol . ”

6) Name of Student: Role & Period:

Dissertation Title:

Tyson Brummer, MS., VMRCVM, Virginia Tech . Major Advisor, and Chair, Graduate Advisory Committee, 2004-2007) “Differential Effects of 17-beta estradiol and Ethynyl Estradiol in young and aged, male and female mice ”

7) Name of Student: Role & Period:

Dissertation Title:

Deena Khan, Ph.D., VMRCVM, VirginiaTech . Major Advisor, and Chair, Graduate Advisory Committee, 200-2012) “ Molecular Basis of Upregulation of Il-17 in Estrogen-Model of Inflammation” “

8) Name of Student:

Role & Period: Dissertation Title (provisional):

Rebecca Phillips, (part-time) Ph.D. candidate in Immunotoxicology, VMRCVM,Virginia Tech . Major Advisor, and Chair, Graduate Advisory Committee,) “Interactions of transcription factors in estrogen Response Elements and Chromatin folding in lymphocytes”

9) Name of Student:

Role & Period:

Dissertation Title:

Samantha Chang, DVM, Ph.D. Inflammation/Immunology, VMRCVM,Virginia Tech . Co-Major Advisor, and Co-Chair, Graduate Advisory Committee, 2007- 2011) (Completed-July 27, 2011)

“A Mechanism for the Metabolic and Inflammatory Alterations

Associated with Low-dose Endotoxemia

10) Name of Student:

Role & Period: Tentative

Catherine Cowan, DVM/Ph.D. dual degree student Inflammation/Immunology, VMRCVM, Virginia Tech. Major Advisor, 2010- to date)

Dissertation Title: “Role of neutrophils in lupus”

11) Name of Student:

Role & Period:

Tentative

Ruoxi Yuan, Ph.D. degree student (Institute of Critical Technology and Applied Science, ICTAS scholar) Inflammation/Immunology, VMRCVM,Virginia Tech . Co-Major Advisor and Co-Chair, 2010- 2016 (graduated on Aug, 16, 2016)

Dissertation Title: “Low dose LPS and disease susceptibility ”

12) Name of Student:

Role & Period:

Michael Edwards, DVM. (Ph.D. candidate) A NIH T32 student working on a Ph.D. degree student (VMRCVM, Virginia Tech . Major Advisor and Chair, 2010- to date

Tentative

Dissertation Title: “Microbiota, nutrition and inflammatory disease ”

13) Name of Student: Mr. Zhuang Wang, MS. candidate, VMRCVM, Virginia Tech

Role & Period: Major Advisor (2016-to date) Tentative Dissertation Title : “Role of miR-182-96-183 cluster in in lupus”.

14) Name of Student: Mr. Alex Murray, MS. candidate, VMRCVM, Virginia Tech

Role & Period: Co-Major Advisor (with Dr. Xie) (2017-to date) Tentative Dissertation Title : “Epigenetic molecular mechanisms in lupus”.

15) Name of Student: Ashton Shiraz, PhD/DVM dual degree candidate. VMRCVM, Virginia Tech

Role & Period: Co-Major Advisor (with Dr. Reilly) (2019-to date) Tentative Dissertation Title : “Metabolic Changes in lupus”.

16) Name of Student: Krishna Shah, PhD degree candidate. VMRCVM, Virginia Tech

Role & Period: Major Advisor (2019-to date) Tentative Dissertation Title : TBA.

Member of Graduate Advisory Committee:

1) Name of Student:

Role & Period: Dissertation Title:

2) Name of Student:

Role & Period: Dissertation Title:

3) Name of Student:

Role & Period: Dissertation Title:

4) Name of Student:

Role & Period: Dissertation Title:

Dr. Lucy Edens MS/Resident,(Equine Immunology) Equine Medical Center,VMRCVM, Dr. Edens holds a DVM degree Graduate Advisory Committee member, 1990-1993, “In vitro cytotoxic activity of equine lymphocytes on equine herpes-1-infected allogenic fibroblasts”.

Ms. Darla Wise, Ph.D. (Immunology), Pathobiology, VMRCVM, Committee member Graduate Advisory Committee member, 1990-94, “Intracellular growth of B.abortus and B. melitensis in murine macrophage-like cell lines and partial characterization of a biologically active extract from B. abortus strain RB51.

Ms. Arden Bond, M.S. (Immunology/Biochemistry), Department of Veterinary Biosciences, VMRCVM Graduate Advisory Committee member, 1990-92, “The Production and characterization of a putative anti-idiotypic

antibody to tumor necrosis factor-”.

Mr. Das Kumuda Chandra, Ph.D. (Cell Biology/Biochemistry), Department of Veterinary Biosciences, VMRCVM, Graduate Advisory Committee member, 1990-1992, “Amelioration of oxidative lung injury by antiarrythmic agents”

5) Name of Student:

Role & Period: Dissertation Title:

Mr. Mathew Walker, Ph.D. (Immunology), Department of Biology, College of Arts and Sciences, Virginia Tech Graduate Advisory Committee member, 1989-94, “Cellular and Molecular Investigations of macrophage alterations by colony stimulating factors during tumor growth”

6) Name of Student:

Role & Period: Dissertation Title:

Mr. David Aleva, Ph.D. (Immunology), Department of Biology, College of Arts and Sciences, Virginia Tech Graduate Advisory Committee member1989- 1994 “Regulation of macrophage activity by tumor growth: Mechanism of immunosuppression”

7) Name of Student:

Role & Period: Dissertation Title:

Ms. Maxine Kellman, DVM., Ph.D. (Immunoparasitology), Dept. of Veterinary Medical Sciences Graduate Advisory Committee member, 1993-97, “Development of an antigen-specific ELISPOT to detect intestinal antibody responses to the swine whipworm, Trichuris suis”.

8) Name of Student:

Role & Period:

Dissertation Title:

Mr. David Warren Mullins Ph.D., College of Arts and Sciences, Virginia Tech Graduate Advisory Committee member, 1994-1998, (Completed Dec 18, 1998)

“Piclitaxel-induced macrophage activities in the tumor-bearing host: Immunologic implications and therapeutic applications”

9) Name of Student: Role & Period:

Dissertation Title:

Dr. Lesley Colby , MS, VMRCVM, Virginia Tech Graduate Advisory Committee member, 1995-1997, (Completed Feb 4, 1997)

“The humoral immune response of Elk (Cervus elaphus nelsoni) and mice to vaccination with Brucella abortus strain RB51”

10) Name of Student: Role & Period:

Dissertation Title:

Dr. Lutz Steffen Goehring DVM, MS, VMRCVM, Virginia Tech. Graduate Advisory Committee member, 1996-1998, (Completed Aug, 1998)

“Equine Protozoal Myeloencephalitis: Preliminary Investigation of protozoan-host interaction in the horse”

11) Name of Student:

Role & Period: Dissertation Title:

Dr. Yongquin, He (Oliver), Ph.D. Student, VMRCVM,Virginia Tech. Dr. He holds a DVM degree Graduate Advisory Committee Member 1996- 2001 “Induction of antibody and cell mediated immune responses by B. abortus strain RB51, Ochrobactrum anthropi and recombinants thereof.”

12) Name of Student: Role & Period: Dissertation Title:

Mr. Mike McConell, MS. (immunology), Department of Biology, Graduate Advisory Committee Member 1997-99 (awarded MS in ’99) “Tumor avoidance of macrophage tumoricidal activity modulation of NF-kB activity”

13) Name of Student:

Role & Period:

Dr. Teri Leichner DVM., MS. (Equine Immunology), MS. Student, Department of Large Animal Clinical Sciences, VMRCVM,Virginia Tech Graduate Advisory Committee Member 1999-2002

Dissertation Title:

14) Name of Student:

Role & Period: Dissertation Title:

15) Name of Student: Role & Period:

Dissertation Title:

16) Name of Student: Role & Period:

Dissertation Title:

17) Name of Student: Role & Period: Dissertation Title:

18) Name of Student: Role & Period: Dissertation Title:

19) Name of Student: Role & Period: Dissertation Title:

20) Name of Student: Role & Period:

Dissertation Title :

21) Name of Student: Role & Period: Dissertation Title :

22) Name of Student:

Role & Period: Dissertation Title :

23) Name of Student:

“Equine COPD: Investigation of the horse as a model for human asthma ”

Dr. Mary Renee Zwallen Prater, Ph.D., VMRCVM, Virginia Tech. Dr. Prater holds a DVM and MS degrees. Graduate Advisory Committee Member, (1999- 2002)

“Immunotoxicity of Dermal Permithrin and cis-Uronic acid Chemical mixtures in environmental health”

Dr. Korawuth Punareewattana (Sine) Ph.D, VMRCVM, Virginia Tech . Graduate Advisory Committee Member, (2000- 2003) (Graduated April 18, 2003)

“Immunoteratologic studies using gene expression analysis“

Fang-Fang Huang, Ph.D., VMRCVM, Virginia Tech . Graduate Advisory Committee Member, (1999- 2004)

(Graduated Sept. 1, 2004)

“Molecular characterization of animal strains of hepatitis E (Graduated

Dec 01, 2004)

Zhi-Feng Sun,, Ph.D., VMRCVM, Virginia Tech . Graduate Advisory Committee Member, (1999- 2004)

“ avian and swine HEV ” (Graduated January. 20, 2005)

Dr. Andrea Conteras Ph.D., VMRCVM,Virginia Tech . Graduate Advisory Committee Member, (1999- 2004)

“Protection induced against Paratuberculosis by recombinant strain RB51” (Graduated July. 30, 2004)

Dr. Shoranda Meade Ph.D., VMRCVM, Virginia Tech . Graduate Advisory Committee Member (2000-2005) “ Molecular mechanisms involving immunity of turkeys against

hemorrahagic enteritis virus ” (Graduated Dec. 7, 2004)

Mr. Elswaifi Shaadi Ph.D., VMRCVM, Virginia Tech . Provisional Graduate Advisory Committee Member (2000-2007) (Completed Nov. 16, 2006)

“ Molecular genetic analysis of lipopolysaccharides from Actinobacillus”.

Mr. Amjad Mustafa Ph.D. VMRCVM, Virginia Tech . Provisional Graduate Advisory Committee Member (2006-12/18/08) “ Dioxin effects on B cell Development”.

Mr. Abdul Gafoor Ph.D. VMRCVM, Virginia Tech . Provisional Graduate Advisory Committee Member (2009-2012) “ B lymphocyte development and function in a NUP98-HOXD13 transgenic mouse model for leukemia ”.

Mr. Samantha Chang DVM, Ph.D. VMRCVM, Virginia Tech .

Role & Period: Provisional Graduate Advisory Committee Member (2009-2011) Dissertation Title : “A Mechanism for the Metabolic and Inflammatory Alterations

Associated with Low-dose Endotoxemia”

24) Name of Student: Mr. Christine Chafin Ph.D. VMRCVM, Virginia Tech .

Role & Period: Graduate Advisory Committee Member (2009-2013 Graduated on 09/24/2013)

Dissertation Title : “MicroRNA-mediated Attenuation of Inflammation in NZB/W Lupus Mice”.

25) Name of Student: Mr. Xiaofeng Liao Ph.D. VMRCVM, Virginia Tech

Role & Period: Graduate Advisory Committee Member (2012-2017- graduated on July 11, 2017)

Dissertation Title : “Role of microbiota in MRL/lpr lupus-prone mice”.

26) Name of Student: Mr. Qinghui Mu Ph.D. candidate in Immunology, VMRCVM, Virginia Tech

Role & Period: Graduate Advisory Committee Member (2012-to date) Dissertation Title : “Analysis of infiltrating DC cells in murine

Lupus nephritis”.

27) Name of Student: Mr. Nick Parkinson Resident/MS. candidate, VMRCVM, Virginia Tech

Role & Period: Graduate Advisory Committee Member (2014-to date) Dissertation Title : “microRNAs in BALs from horses”.

28) Name of Student: Leila Abdul Hamid, PhD candidate, VMRCVM, Virginia Tech

Role & Period: Graduate Advisory Committee Member (2018-to date) Dissertation Title : “Environmental regulation of lupus ”.

TRAINING RESIDENTS OR POST-DOCTORAL SCHOLARS:

Responsible for training the following scholars in immunology

1. Dr. Li-Zi Pan, MD, Visiting Scientist from The Republic of China. 1984-1985. UTHSCSA, Texas.

2. Dr. Jong-Rern Chen, M.D., Visiting Scientist from Taiwan. 1987-1988. UTHSCSA, Texas.

3. Dr. D. Olive, M.D., Dept. of Obstetrics and Gynecology.1987-88 UTHSCSA, Texas.

4. Dr. Robert Gogal Jr. DVM, Research Associate in Immunology, 1992-1997, Dept. Pathobiology, VMRCVM.

5. Dr. Lucy Edens, DVM, MS (Large Animal Resident)Equine Medical Center, VMRCVM. 1990-1993. Leesburg, VA (M.S/ Resident Program: Research committee member).

6. Dr. Lutz Goehring,, DVM., MS (Large Animal resident) 1996-98. 7. Dr. Bruce Hissong, Ph.D. (Immunology) 1996-2000 8. Dr. Ebru Karuzoglu,Ph.D. (Immunology), 2005 to date 9. Dr. Rujuan Dai, Research Scientist (Immunology), 2005- 2012 10. Dr. Rujuan Dai, Research Assistant Professor (Immunol), 2012-to date

11. Dr. Zhihong Liang, Visiting Research Scientist, 2012-2013

12. Dr. Abdul Gafoor Puthiyaveetil, Research Fellow at University Health Network, Toronto, Ontorio, Canada,

13. Dr. Deena Khan, Postdoc (Immunology), 2012-2015 14. Dr Ran Lu, Postdoc (Immunology), 2013-2016

Current positions held by former graduate students:

• Dr. Li-Zi Pan : Present Position: Asst. Professor of Medicine, Gungzhon, China

• Dr. Jong-Rern Chen: Associate Professor in Internal Medicine, Kaohsiung Medical College, Taiwan.

• Dr. David Olive: Associate Professor of Obstetrics and Gynecology, UTHSCSA, Texas.

• Dr. Lucy Edens: Present Position: Assistant professor, University of Florida

• Dr. Daniela Verthelyi: Present Position: Chief, Laboratory of Immunology, Division of Therapeutic Proteins, Center for Biologics Evaluation and Research of FDA

• Ms. Arden Bond: Present Position: Research Associate, NCSU, NC

• Dr. David Alleva, Sr. Manager, Emergent Biosolutions. Boston, University, MA

• Dr. Robert Gogal Jr. DVM, Professor, University of Georgia, Vet Med-

• Dr. Matt Walker, Associate Professor, Central Arkansas University.

• Dr. Kamuda Das, Assistant Professor, Texas Tech., Lubbock, TX

• Ms. Kelcey Becker, Ph.D. Student, Texas A & M

• Dr. Ebru Karpuzoglu, Postdoc, Res. Assist. Prof. University of Georgia

• Mrs. Jillian Calemine, Research Associate, Depts. of Immunology and Protein Chemistry, Genetech, Inc., 1 DNA Way, South San Francisco, CA

• Dr. Tyson Brummer, completed DVM and is now a Senior VMRCVM resident,

• Dr. Liang Zhihong, Associate Professor, College of Food Science and Nutritional Engineering Box 302, China Agricultural University No.17 Qinghua Donglu, Haidian District, Beijing, P.R. China, 100083

• Dr. Abdul Gafoor Puthiyaveetil, Assistant Professor of Biotechnology, American University of Ras Al Khimah School of Arts and Science, Dubai ; Current position Research Fellow at University Health Network, Toronto, Ontorio, Canada,

• Dr. Deena Khan, Ohio State University, Ohio