biomart update
DESCRIPTION
Title: Biomart 2007Author: Arek KasprzykTRANSCRIPT
BioMart 2007
Arek KasprzykEuropean Bioinformatics InstituteBOSC Vienna, July 2007
Data Flow
Mart
JAVA
PERL
Source data
DAS
WebGUI
Commandline
Desktop GUI
WebService
Data Flow
JAVA
PERLMartDAS
WebGUI
Commandline
Desktop GUI
WebService
Admin Tools
Recent developments (0.4- 0.6)
• MartBuilder
• MartView
• Web services
• API
• DAS
• Central Server
• More deployers
Data Flow
Mart
JAVA
PERL
Source data
DAS
WebGUI
Commandline
Desktop GUI
WebService
MartBuilder
MartBuilder
MartBuilder
MartView
APImy $initializer = BioMart::Initializer->new('registryFile'=>$confFile);my $registry = $initializer->getRegistry;my $query = BioMart::Query->new('registry'=>$registry,'virtualSchemaName'=>’central_server_1');
$query->setDataset("hsapiens_gene_ensembl"); $query->addFilter("chromosome_name", [”1"]);
$query->addAttribute("ensembl_gene_id"); $query->addAttribute("ensembl_transcript_id"); $query->addAttribute(”ensembl_peptide_id"); $query->setDataset(“msd”); $query->addFilter(”experiment_type", [”NMR"]); $query->addAttribute("pdb_id"); $query->addAttribute(”resolution");
$query->addAttribute(”release_date"); $query->addAttribute(”header");
my $query_runner = BioMart::QueryRunner->new(); $query_runner->execute($query); $query_runner->printResults();
Web service
<Query virtualSchemaName="central_server_1">
<Dataset name="hsapiens_gene_ensembl" > <Filter name="chromosome_name" value="1"/><Attribute name="ensembl_gene_id"/><Attribute name="ensembl_transcript_id"/><Attribute name="ensembl_peptide_id"/> </Dataset>
<Dataset name="msd"> <Filter name="experiment_type" value=”NMR"/><Attribute name="pdb_id"/><Attribute name=”resolution"/><Attribute name=”release_date"/><Attribute name=”header"/>
</Dataset></Query>
MartService
• Meta data(GET)– Marts
– Datasets
– Configuration
• Queries (POST)
Meta data
http://www.mycompany.com/mypath/martservice?
• Martstype=registry
• Datasetstype=datasets&mart=mymart
• Configurationtype=configuration&dataset=mydataset
Query
<?xml version="1.0" encoding="UTF-8"?> <!DOCTYPE Query><Query virtualSchemaName = "default" count = "" softwareVersion = "0.5" > <Dataset name="hsapiens_gene_ensembl" >
<Attribute name="ensembl_gene_id"/><Attribute name="ensembl_transcript_id"/><Filter name="chromosome_name" value="1"/><Filter name="band_end" value=”p36.33"/><Filter name="band_start" value=”q44"/>
</Dataset>
<Dataset name="msd"><Attribute name="pdb_id"/><Attribute name=”experiment_type"/><Filter name="experiment_type" value=”NMR"/>
</Dataset></Query>
wget -q 'http://www.biomart.org/biomart/martservice?query=
-O 5utr.dat
Results
• Ordered according to 1. Datasets
2. Attributes
• Default Format TSV– Can be altered by specifying a formatter
Genomic data
Uniprot, MSD, ArrayExpress
Model organism databases
Developmental models
Proteomics
Name Fragment Position Alleles strand
SNP1 AL139258 1659852 T/A 1
SNP2 NT_25698 2569873 C/T -1
SNP3 chr13 1125698 C/G 1
Data conversion and integration
Ensembl
HapMap
NCBI
UCSC
Priopriatery data
Diabetes-Gene Association DataBase
Combined proprietary and
public data
Genetics of Infectious and Autoimmune Diseases, Pasteur Institute, INSERM U730, Paris, France.
Target SNP selection for the study of
type 1 diabetes (T1D), malaria and dengue
CAPRISA understanding HIV pathogenesis and epidemiology as
well as HIV/AIDS treatment and prevention
Clinical Data
MID
Cellular ImmunityHumoral Immunity HLA TypingSequence &
Sequence Related
Pipeline
Unilever
• Human study to evaluate Omics in assessing safety indicators
• Study of skin inflammation in response to detergent
• Skin samples taken and analyzed with multiple Omics techniques. – Blood– Skin biopsy– Microdialysis
1. Filter 2. Attributes
3. Results
Use Example 1 All genes in the human genome
up-regulated in Pancreatic Adenocarcinomas (PDACs) vs Normal Pancreas (ND))
1. Filter 2. Attributes
3. Results
Use Example 2 all upstream sequences for all genes on chromosome 1
up-regulated in Pancreatic Adenocarcinomas (PDACs) vs Normal Pancreas (ND))
1. Filter2. Attributes
Use Example 3
3. Results
Just Finished my experiment and would like to get the overlaps between my results and those reported in previous studies !
Web service
Perl
DAS
Bioconductor package biomaRt
Galaxy
Taverna
Central Server (www.biomart.org)
www.biomart.org/biomart/martservice
Future plans
New configuration system
• Normalized– Based on a partition table concept– Unified pointer system -> relational attribute– Configuration merge - implicit federation– Write to the db
• Run time slice and dice of a registry object rather than combinatorial pre-compilation
New configuration system
• Scalability– Updates and maintenance of large configurations
– Run time server scalability (cache and memory)
– Scalable for multiple mart users (single instance - security)
– Scalable for alternative configurations (new MartGUI framework)
New MartGUI framework
• Components– Alternative DS Configurations– Alternative GUIs (MView, MQForm,MSForm etc)– Alternative Analyzers/Vizualizers (optional install)
• Extensible – Custom extensions to the components
• Common interface – Formatters, DAS, Analyzers, Visualizers– Importable/Exportable pair interface
New GUI framework
• Old ‘GUI unit’:– full registry+MartView+default formatters– Customization limited to colors and headers
• New ‘GUI unit’:- RegistrySlice+ MartGUI+Visualizer/Analyzer- Combine units into your unique functional
environment- Functional level customization
New GUI framework
Gene Id conversion
Functional annotation
Compare two gene lists
Analyze gene list Draw distribution
Full search
Draw bla bla chart
Home
Welcome to my data mining website
SITE HEADER
New GUI framework
Gene Id conversion
Functional annotation
Compare two gene lists
Analyze gene list Genbank
TremblUniprot
Submit
Draw distribution
Full search
paste your ids here
Draw bla bla chart
Hugo
Home
SITE HEADER
New GUI framework
Home Gene Id converterFu
Full search
Welcome to my data mining website
New GUI framework
Hugo Genebank
Uniprot Swissprot
Submit
paste your ids here
HomeFu
Full searchGene Id conversion
Cytogenetic distribution of pancreatic cancer genes satisfying my query (histogram)
Cytogenetic distribution of pancreatic cancer genes satisfying my query (ideogram)
Cytogenetic distribution of chromosomal aberrations in pancreatic cancer
New GUI framework
New GUI framework
New configuration tool
• MartConfigurator– Handles a complete registry object
– Defines GUI units
– Automated service discovery
– Manual link override
– Automated updates for large configurations
– Improved user interaction
Credits• Martians
– Syed Haider
– Richard Holland
– Damian Smedley
• Contributors– Steffen Durinck (NCI, NIH)
– Eric Just (Northwestern University)
– Don Gilbert (Indiana University)
– Darin London (Duke University)
– Will Spooner (CSHL)
– Gudmundur Thorisson (CSHL)
– Benoit Ballester (Universite de la Mediterranee)
– James Smith (Ensembl)
– Arne Stabenau (Ensembl)
– Andreas Kahari (Ensembl)
– Craig Melsopp (Ensembl)
– Katerina Tzouvara (EBI)
– Paul Donlon (Unilever)