biology notes chapter four complete
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Biology Notes 07/12/2009 22:50:00
4.1- Microscopes reveal the world of the cell
Vocabulary-
Light microscope (LM)
o
An optical instrument with lensesthat refract (bend) visible light to
magnify images and project them
into a viewer's eye or onto
photographic film.
Cell theory
o The theory that all living things
are composed of cells and that all
cells come from other cells.
Electron microscope
o An instrument that focuses an
electron beam through, or onto
the surface of, a specimen. An
electron microscope achieves a
hundredfold greater resolution
than a light microscope.
Scanning electron microscope
o A microscope that uses an
electron beam to study the
surface architecture of a cell or other specimen. Transmission electron microscope (TEM)
o A microscope that uses an electron beam to study the internal structure of
thinly sectioned specimens.
4.2- Most cells are microscopic
There is a reason that our knowledge of cells depended on the development of
the microscope.
Most cells cannot be seen with the naked eye.
The logistics of carrying out a cells function sets limits on its size
o Minimum: Must be big enough to house DNA, protein molecules, and
internal structures to survive and reproduce
o Maximum: required to have enough surface area to obtain adequate
nutrients and oxygen and dispose of wastes
o Also limited to the distance these materials must diffuse in a cell
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Larger cells have more surface area than smaller cells, but less area in
comparison to their volume than smaller cells
4.3- Prokaryotic cells are structurally simpler than eukaryotic cells
Vocabulary
Prokaryotic cells
o A type of cell lacking a membrane-enclosed nucleus and other membrane-
enclosed organelles; found only in the domains Bacteria and Archaea.
Eukaryotic cells
o A type of cell that has a membrane-enclosed nucleus and other
membrane-enclosed organelles. All organisms except bacteria and
archaea are composed of eukaryotic cells.
Plasma membrane
o The membrane that sets a cell off from its surroundings and acts as a
selective barrier to the passage of ions and molecules into and out of the
cell; consists of a phospholipid bilayer in which are embedded molecules
of protein and cholesterol.
Chromosomes
o A threadlike, gene-carrying structure found in the nucleus of a eukaryotic
cell and most visible during mitosis and meiosis; also, the main gene-
carrying structure of a prokaryotic cell. Chromosomes consist of
chromatin, a combination of DNA and protein.
Ribosomes
o A cell structure consisting of RNA and protein organized into two subunits
and functioning as the site of protein synthesis in the cytoplasm. The
ribosomal subunits are constructed in the nucleolus.
Cytoplasm
o Everything inside a cell between the plasma membrane and the nucleus;
consists of a semifluid medium and organelles.
Nucleoid
o A dense region of DNA in a prokaryotic cell.
Flagella
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o Plural, flagella) A long cellular appendage specialized for locomotion. The
flagella of prokaryotes and eukaryotes differ in both structure and
function. Like cilia, eukaryotic flagella have a 9 + 2 arrangement of
microtubules covered by the cell's plasma membrane.
Two kinds of structurally different cells have evolved over time.
Prokaryotic cells
o Bacteria and archaea
Eukaryotic cells
o Everything else, ie; protists, fungi, plants, and animal
All cells have several basic features in common
Bounded by a plasma membrane
Have chromosomes
o Carry genes of DNA
Contain ribosomes
o Tiny structures that
make proteins according
to gene instruction
Have cytoplasm
o Area between nucleus
and cell membrane
Eukaryotic cells
Membrane bound nucleus
o Houses DNA
Contain many more membrane bound organelles than prokaryotic cells
Usually about 10x the size of prokaryotic cell
Prokaryotic cells
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Nucleoid
o Where DNA is stored in a prokaryotic cells
No membrane surrounds DNA
Ribosomes
o
Smallero Differ
Basis for the action of some antibiotics
Have a rigid, chemically complex cell wall
o Protects and helps maintain shape
Flagella
o Long projections that propel the cell through its liquid environment
4.4- Eukaryotic cells are portioned into functional compartments
Vocabulary
Organelles
o A membrane-enclosed structure with a specialized function within a cell.
Cellular metabolism
o The chemical activities of cells
Plant Cell
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4.5- The structure of membrane correlates with their function
For all cells, the plasma membrane forms a boundary between the living cell and its
surroundings and controls the traffic materials into and out of the cell.
4.6- The nucleus is the cells genetic control center
Vocabulary
Nucleus
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o (plural, nuclei) (1) An atom's central core, containing protons and
neutrons. (2) The genetic control center of a eukaryotic cell.
Chromatin
o The complex of DNA and proteins that constitutes eukaryotic
chromosomes; often used to refer to the diffuse, very extended formtaken by chromosomes when a cell is not dividing.
Nuclear envelope
o A double membrane, perforated with pores, which encloses the nucleus
and separates it from the rest of the eukaryotic cell.
Nucleolus
o A structure within the nucleus of a eukaryotic cell where ribosomal RNA is
made and assembled with proteins imported from the cytoplasm to make
ribosomal subunits.
The nucleus contains most of the calls DNA and controls the cells activities by
directing protein synthesis
Eukaryotic cells
Made up of chromatin
o A complex of DNA and
protein
o Appears as a diffuse
mass
o As a cell prepares to
divide, the DNA is
copied and the thin
chromatin fibers coil up,
becoming thick enough
to be seen with a light microscope
Nuclear envelope
o Encloses the nucleus
o A double membrane perforated with protein-lined pores
o Control the flow materials into and out of the nucleus
o Connects with the cells network called the endoplasmic reticulum Nucleolus
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o A prominent structure in the nucleus
o A special type of RNA called ribosomal RNA
Synthesized according to instruction in DNA
4.7- Ribosomes make proteins for use in the cell and export
Ribosomes are the cellular components that carry out protein synthesis.
High rated of proteins, means more ribosomes
Ribosomes found in two areas
Free ribosomes- suspended in fluid
Bound ribosomes- attached to the endoplasmic reticulum, and nuclear envelope
Structurally identical, and ribosomes can alternate between the two.
Most proteins made on free ribosomes function with cytoplasm
Ex. Enzymes that catalyze the first steps of sugar breakdown
Cells that specialize in protein secretion, have a high proportion of bound ribosomes
to free ribosomes
4.8- Overview: Many cell organelles are connected through the end membrane
system
Vocabulary
Endomembrane system
o A network of membranes inside and around a eukaryotic cell, related
either through direct physical contact or by the transfer of membranous
vesicles.
Endoplasmic reticulum
o An extensive membranous network in a eukaryotic cell, continuous with
the outer nuclear membrane and composed of ribosome-studded (rough)
and ribosome-free (smooth) regions. See also rough ER; smooth ER.
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4.9- The endoplasmic reticulum is a biosynthetic factory
Vocabulary-
Smooth endoplasmic reticulum
o A network of interconnected membranous tubules in a eukaryotic cell's
cytoplasm. Smooth ER lacks ribosomes.
Rough endoplasmic reticulum
o A network of interconnected membranous sacs in a eukaryotic cell's
cytoplasm. Rough ER membranes are studded with ribosomes that make
membrane proteins and secretory proteins.
Glycoprotein
o A macromolecule consisting of one or more polypeptides linked to short
chains of sugars.
Transport Vesicle
o A tiny membranous sac in a cell's cytoplasm carrying molecules produced
by the cell. The vesicle buds from the endoplasmic reticulum or Golgi and
eventually fuses with another membranous organelle or the plasma
membrane, releasing its contents.
Smooth ER The Smooth ER of various cell types functions in diverse metabolic processes.
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o Enzymes are important in the synthesis of lipids, including oils,
phospholipids and steroids.
Liver
o Certain enzymes in the smooth ER help process drugs and other
potentially harmful substances.o As cells are exposed to chemicals, the amount of smooth ER increases
Store calcium ions
o Muscle cells
o Rush of calcium into cell causes the cell to contract
Rough ER
One of the functions of Rough ER is to make more membrane
o Phospholipids made by enzymes are inserted into membrane
Therefore enlarges
Part of membrane is transferred to other parts of the
endomembrane system by vesicles.
Bound ribosomes produce proteins to be inserted into membrane, used in cell, or
transported out of cell
Protein synthesis
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4.10- The Golgi apparatus finishes, sorts and ships cell products
Vocabulary-
Golgi apparatuso An organelle in eukaryotic cells consisting of stacks of membranous sacs
that modify, store, and ship products of the endoplasmic reticulum.
After leaving the ER, many transport vesicles travel to the Golgi apparatus
Discovered by Camillo Golgi
Flattened sacs on top of each other.
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Cell can contain few, or hundreds
o Depends on how active the cell is in secreting proteins
Functions
Receives and modifies products from ER One side serves as a receiving dock, and vesicles join the Golgi apparatus
Exit from the other side, and travel elsewhere.
Products are usually modified during their transit in the Golgi.
Sacs nature as they move from receiving side to shipping side
Vesicles become part of plasma membrane, or exit from it.
4.11- Lysosomes are digestive compartments within a cell
Vocabulary-
Lysosome
o A digestive organelle in eukaryotic cells; contains hydrolytic enzymes that
digest the cell's food and wastes.
A lysosomes consists of digestive enzymes enclosed in a membranous sac
Enzymes and membranes made by Rough ER and then transported to Golgi for
more processing.
Illustrate compartmentalization
Enclosed a compartment in which digestive enzymes are provided with an acidic
environment and are safely isolated from the rest of the cell
Several digestive functions
Many protists engulf food particles into cytoplasmic sacs called food vacuoles
With help of lysosomes, cell continually renews itself
4.12- Vacuoles function in the general maintenance of the cell
Vocabulary-
Vacuoleso A membrane-enclosed sac that is part of the endomembrane system of a
eukaryotic cell, having diverse functions.
Central Vacuole
o A membrane-enclosed sac occupying most of the interior of a mature
plant cell, having diverse roles in reproduction, growth, and development.
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Vacuoles are membranous sacs that have a variety of functions.
Central vacuole
o Has hydrolytic functions like a lysosomes
o Helps the cell grow in size by absorbing water and enlarging
o
Can store vital chemicals or waste productso
Without a way to get rid of excess water, the cell would become too dilute to support
life, and eventually would swell and burst.
4.13- A review of the structure involved in the manufacturing and breakdown
Vocabulary-
Peroxisome
o An organelle containing
enzymes that transfer
hydrogen from various
substrates to oxygen,
producing and then
degrading hydrogen
peroxide.
Relationships
Structural
o Between
Nuclear envelope
Rough ER
Smooth ER
Functional
o Membrane and proteins produced by the ER travel in transport vesicles to
the Golgi and from there to other areas.
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o Some become lysosomes or vacuoles
Transport vesicles
Carry secretory proteins to the protein membrane
Peroxisome An organelle containing enzymes that transfer hydrogen from various substrates
to oxygen, producing and then degrading hydrogen peroxide.
Not a part of the endomembrane system
Breaks down fatty acids to be used as fuel and detoxify
4.14- Mitochondria harvest chemical energy from bond
Vocabulary-
Mitochondria
o An organelle in eukaryotic cells where cellular respiration occurs. Enclosed
by two concentric membranes, it is where most of the cell's ATP is made.
Intermembrane space
o One of the two fluid-filled internal compartments of the mitochondrion.
The intermembrane space is the narrow region between the inner and
outer membranes.
Mitochondrial matrix
o The fluid contained within the inner membrane of a mitochondrion.
Mitochondria are organelles that carry out cellular respiration in nearly all eukaryotic cells
Convert sugars and foods to ATP (adenine triphosphate)
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o Main source of energy for cellular work
Structure suits function
Two phospholipids bilayers each with unique embedded proteins
Two internal componentso Intermembrane space
The narrow region between the inner and outer membranes.
o Mitochondrial matrix
Enclosed by inner membrane
Contains mitochondrial DNA, and ribosomes
Also includes many enzymes that catalyze some reactions of
cellular respiration
o Inner membrane
Highly folded
Embedded with molecules of protein that make ATP
o Cristae
Folds
4.15- Chloroplasts convert solar energy to chemical energy
Vocabulary-
Chloroplasts
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o An organelle found in plants and photosynthetic protists that absorbs
sunlight and uses it to drive the synthesis of organic molecules (sugars)
from carbon dioxide and water.
Stroma
o
The fluid of the chloroplast surrounding the thylakoid membrane; involvedin the synthesis of organic molecules from carbon dioxide and water;
Sugars are made in the stroma by the enzymes of the Calvin cycle.
Thylakoids
o One of a number of disk-shaped membranous sacs inside a chloroplast.
Thylakoid membranes contain chlorophyll and the enzymes of the light
reactions of photosynthesis. A stack of thylakoids is called a granum.
Granum
o A stack of hollow disks formed of thylakoid membrane in a chloroplast.
Grana are the sites where light energy is trapped by chlorophyll and
converted to chemical energy during the light reactions of photosynthesis.
4.16- Mitochondria and chloroplasts evolved by endosymbiosis
Vocabulary-
Endosymbiosis
o A process by which the mitochondria and chloroplasts of eukaryotic cells
probably evolved from symbiotic associations between small prokaryotic
cells living inside larger cells.
4.17- The cells internal skeleton helps organize its structure and activities
Vocabulary-
Cytoskeleton
o A network of protein fibers in the cytoplasm of a eukaryotic cell; includes
microfilaments, intermediate filaments, and microtubules.
Microfilaments
o The thinnest of the three main kinds of protein fibers making up the
cytoskeleton of a eukaryotic cell; a solid, helical rod composed of the
globular protein actin.
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Intermediate filaments
o An intermediate-sized protein fiber that is one of the three main kinds of
fibers making up the cytoskeleton of eukaryotic cells. Intermediate
filaments are ropelike, made of fibrous proteins.
Microtubuleso The thickest of the three main kinds of fibers making up the cytoskeleton
of a eukaryotic cell; a straight, hollow tube made of globular proteins
called tubulins. Microtubules form the basis of the structure and
movement of cilia and flagella.
4.18- Cilia and flagella move when microtubules bend
Vocabulary-
Cilia
o A short cellular appendage specialized for locomotion formed from a core
of nine outer doublet microtubules and two single microtubules covered by
the cell's plasma membrane.
Basal Body
o A eukaryotic cell organelle consisting of a 9 + 0 arrangement of
microtubule triplets; may organize the microtubule assembly of a cilium or
flagellum; structurally identical to a centriole.
Microtubular structures that extend outside some cells and that assist in moving the
cell or the cell's surroundings.
Flagella are much longer than cilia.
When a cell has cilia, their number is large, whereas a cell will have few flagella,
or a single flagellum.
Many protists have cilia, and the sperm of many plants and animals have flagella.
Flagella and cilia are assembled from tubulin subunits organized to form a circular
arrangement of nine pairs ofmicrotubules attached to a central pair.
Flagella and cilia bend to cause movement of either the cell or the surroundings.
The bending uses energy derived from the hydrolysis ofATP.
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4.19-Problems with sperm motility may be environmental or genetic
When the flagellum dont flip correctly, the mommy does not get pregnant.
4.20- The extracellular matrix of animal cells functions in support, movement, and
regulation
Vocabulary-
Extracellular matrix
o A substance in which the cells of an animal tissue are embedded; consists
of protein and polysaccharides.
Integrins
o A transmembrane protein that interconnects the extracellular matrix and
the cytoskeleton.
4.21- Three types of cell junctions are found in animal tissues
Tight junctions
Bind cells together
Found predominantly in epithelial tissues
o Such as the lining of the intestines.
Tight junctions form a barrier that prevents fluids from moving between the
intestinal space and the space between cells.
Animal cells in multicellular tissues are usually joined by tight junctions,
desmosomes (also called anchoring junctions), and gap junctions (also called
communicating junctions). Plant cells are often joined by plasmodesmata. Each
type of junction has specialized functions, such as preventing fluid leakage
between membranes or allowing the free passage of small molecules and ions
between adjacent cells.
Anchoring junctions
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Desmosomes
appear as thickened patches in the cell membrane region between two cells
contain specialized proteins, such as keratin (the same protein found in
fingernails and hair), that increase the rigidity of tissues.
Desmosomes such as those found in epithelium, smooth muscle, and manyother animal tissues are buttonlike junctions that bind cells together and also
function as anchors for fibers in the cytoskeleton.
Gap junctions
Communicating junctions
Two cells are separated by a small gap, which is bridged by specialized channels
that allow the passage of water and small molecules.
Help coordinate the activities of adjacent cells
o a hormone that stimulates one cell will often activate adjacent cells as well
by the passage of intracellular signals through gap junctions.
Plant cells, unlike animal cells, are surrounded by thick walls that form rigid tissues.
They do not need the junctions found in animal tissues.
o But some higher plant cells are interconnected by plasmodesmata
tubelike structures that penetrate through cell walls and form fluid
connections between adjacent cells.
o Like gap junctions in animal cells, plamodesmata allow the free exchange
of small molecules and help coordinate the activities of neighboring cells.
4.22- Cell walls enclose and support plant cells
Vocabulary-
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Cell wall
o A protective layer external to the plasma membrane in plant cells,
bacteria, fungi, and some protists; protects the cell and helps maintain its
shape.
Plasmodesmatao An open channel in a plant cell wall through which strands of cytoplasm
connect from adjacent cells.
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