biology - manatee school for the arts / · pdf file · 2016-11-29biology a tour of...
TRANSCRIPT
Introduction to Cells
https://vimeo.com/37107992?ref=em-share
https://www.brainpop.com/science/cellularlif
eandgenetics/cells/
• A cell is the smallest unit of life.
• They can vary in size, shape and function (structure determines function).
• The light microscope led the way to knowledge of the cell.
http://www.microscope-microscope.org/basic/microscope-
images/138-microscopes-lg.jpg
http://www.smithsonianchannel.com/sc/web/show/3379126/aliens-inside-us
Microscopes:
• A light (COMPOUND)
microscope magnifies objects (specimens) ~1000x their size. Most cellular structures CANNOT be seen.
• You will work with a light microscope in the lab.
http://www.dsbn.edu.on.ca/schools/Westlane/Scien
ce/simon/SBI3C1/micro.gif
• Electron microscopes give more detail and
magnify a million times the object’s size.
• However, the organism dies when using an
electron microscope.
There are 2 types of electron microscopes:
• A scanning electron microscope (SEM) gives
a 3D image of a specimen/object.
• A transmission electron microscope (TEM)
transmits electrons to view the interior of an
object.
http://img72.imageshack.us/img72/2392/071003100546198ddedh5.jpg http://www.st-andrews.ac.uk/~dclogan/Fig3.jpg
CELL THEORY
https://www.khanacademy.org/science/biology/structure-of-a-
cell/introduction-to-cells/v/cell-theory
A history lesson:
• Robert Hooke (1665) named the cell after looking at cork under the microscope.
http://www.google.com/imgres
• Anton van Leeuwenhoek (LAY-von-hohk) (1670’s) developed a simple light microscope & discovered unicellular organisms and called them “little beasties”.
• Rudolf Virchow (1855) stated that all cells came from pre-existing cells (a.k.a. Cell Theory).
http://www.google.com/imgres
The Cell Theory (Virchow) has 3 basic
principles:
1. Cells are the basic
units of life.
2. All organisms are
made of 1 or more
cells.
3. All cells arise from
existing cells.
http://www.leksikon.org/images/virchow_rudolf.jpg
http://www.dmturner.org/Teacher/Pictures/Cell%20reproduction.jpg
Cell Structures:
• All cells have an enclosure called a cell, or plasma membrane.
• This functions as the gate keeper and controls what enters and exits the cell.
• It is mainly composed of phospholipids and proteins. http://www.google.com/imgres
• Within the cell is the cytoplasm. This is a semifluid substance that contains the organelles.
• The organelles are small structures that have specific functions within the cells.
http://www.google.com/imgres
• The cytoskeleton is a protein network within the cytoplasm that helps support the cell and helps the cell maintain or change its shape. It also
– Anchors organelles
– Enables the cell to move
– Allows materials to move throughout the cell
– Composed of microtubules & microfilaments
http://www.google.com/imgres
• The nucleus is the control center of the cell
– It is surrounded by the nuclear envelope
– It contains the chromosomes. There are 46 chromosomes in the human nucleus (in every cell of the human body). The chromosomes are the DNA (genetic material).
• The nucleus is only found in eukaryotic cells!
http://www.google.com/imgres
• The nucleus
contains the
nucleolus
(if more than 1,
nucleoli).
• Nucleolus:
makes
ribosomes
• Ribosomes
make proteins.
http://www.google.com/imgres
2 Types of Cells Prokaryotic Cells
• Bacteria
• NO organelles
(membrane-bound
structures)
• Contained w/in cell
membrane & cell wall,
contain ribosomes, 1
circular chromosome in
nucleoid region (NO
nucleus) & plasmids
(extra pieces of DNA)
Eukaryotic Cells
• Protists, Fungi, Plants,
& Animals
• Contained w/in cell
membrane (may have a
cell wall)
• Contain nucleus &
other membrane-bound
organelles
• Means ‘true kernel’
There are 2 types of cells:
1. Prokaryotes:
These are bacteria
(in Kingdoms
Archaebacteria
and Eubacteria).
– They are unicellular
organisms.
– These were the first
cells. They are very
small cells and are
very simple cells.
http://www.singleton-associates.org/gifs/cell.jpg
2. Eukaryotes: found in
all other kingdoms.
– These have a nucleus
(as well as a cell
membrane and the
majority of the
organelles being
discussed, depending
on the type of
organism).
http://www.google.com/imgres
ANIMAL CELLS:
http://viewpure.com/cj8dDTHGJBY?start=0&end=0
PLANT CELLS:
http://viewpure.com/9UvlqAVCoqY?start=0&end=0
• Ribosomes make proteins from amino acids;
can be found suspended within the
cytoplasm or attached to the endoplasmic
reticulum. (not really organelles; these are
cellular components)
• The endoplasmic reticulum (ER) is a network
of membrane that produces materials for the
cell. There are 2 types:
– The rough ER contains ribosomes and functions
in protein synthesis and makes new cell
membrane.
– The smooth ER makes lipids, process
carbohydrates and breaks down toxins.
• The golgi apparatus is composed of flat
membranous save that modify, package and
distribute molecules (warehouse of the cell).
http://www.google.com/imgres
• Vacuoles are membrane-
bound organelles that
have various functions.
– Some store food, water,
proteins, ions, or wastes.
Generally these are large
and centralized.
• Lysosomes contain
digestive enzymes that
break down large
molecules and old
organelles that the cell no
longer needs.
Vacuole
http://www.google.com/imgres
• Chloroplasts, double-membrane-bound organelles, perform photosynthesis.
– This is the process of making sugar (synthesis) in the presence of light (photo).
– Plants (some bacteria & protists) make their own food (a.k.a. autotrophic).
http://www.daviddarling.info/images/chloroplast.jpg
• The mitochondrion is the “powerhouse of the cell” b/c it changes stored enter from food into useable chemical energy (ATP) for chemical reactions.
– ATP = adenosine triphosphate (energy ‘currency’ of cells)
– The chemical reactions are cellular respiration.
http://scienceblogs.com/worldsfair/Mitochondria.jpg
• Cilia are short hair-
like projections
that are in the
surface on the cell
usually in large
number. (NOT
organelles)
– Beat in unison and
aid in the cell’s
movement or in the
movement of fluid
over the cell.
Cellular Structures
http://www.google.com/imgres
• Flagella are long tail-like
projections that are on
the surface of the cell.
(NOT organelles)
– Usually 1 to 3 of these.
– In prokaryotic cells, they
spin like propellers.
– In eukaryotic cells, they
move like whips.
http://www.google.com/imgres
Plants differ from animal
cells. In plants:
• A cell wall surrounds the
cell membrane.
• The cell wall is a rigid outer
covering that protects and
maintains the shape of the
plant cell.
• Fungi, algae (a type of
protist) and bacteria also
have cell walls but the
composition is different.
Cell Wall
http://www.google.com/imgres
• Plants LACK lysosomes and centrioles.
• Animals are heterotrophic, meaning
they must consume food.
Animal cells:
• LACK a cell wall but have cytoskeletons
for structural support.
• LACK chloroplasts
• Contain small vacuoles (instead of a
large centralized one)
• Have lysosomes
BOTH Animal and Plant Cells Contain:
• Organelles previously mentioned
(nucleus, ER, mitochondria, ER,
ribosomes, etc)
• Cell membranes
• DNA (in chromosomes)
REVIEW:
http://www.cellsalive.com/cells/cell_mod
el.htm
SEMI-PERMEABLE MEMBRANE • Cell/plasma
membrane is composed of a phospholipid bilayer (2 layers of phospholipids) with proteins interspersed.
• Phospholipids have a hydrophilic head & hydrophobic tail
• Fluid & flexible
http://www.bioteach.ubc.ca/Bio-
industry/Inex/graphics/phospholipid.gif
Membrane Functions • Cell membranes are semipermeable. This
means that some things pass through the membrane while others cannot pass through (this depends on the size & charge of the molecule).
• Passive transport is the movement of a substance across a membrane without energy input.
• Active transport is the movement of a substance across a membrane with the input of energy.
• http://www.sumanasinc.com/webcontent/animations/content/diffusion.html
• Concentration=[ ].
• Molecules move from a higher [ ] gradient to a lower [ ]
gradient.
• A [ ] gradient is the difference between the [ ] of a particular
molecule in 1 area and its [ ] in an adjacent area.
• The rate of diffusion depends on temperature and size of
molecules involved (molecules move faster at higher temperatures
and smaller molecules move faster than larger molecules).
• Once molecules are dispersed evenly, DYNAMICequilibrium is
reached and diffusion stops.
CLICK ON LINK:
http://highered.mheducation.com/sites/007249585
5/student_view0/chapter2/animation__how_diffu
sion_works.html
• Diffusion is the movement of molecules from
a higher concentration to a lower
concentration.
Passive Transport
http://www.google.com/imgres
• Facilitated diffusion is the diffusion of
molecules with the help of a carrier
protein embedded within a cell
membrane. CLICK ON LINK:
• http://highered.mheducation.com/sites/00
72495855/student_view0/chapter2/animat
ion__how_facilitated_diffusion_works.htm
http://www.google.com/imgres
• Osmosis is the diffusion of water.
• Diffusion, facilitated diffusion and osmosis
are all type of passive transport. These do
NOT require energy (occur spontaneously).
http://www.biologycorner.com/resources/osmosis.jpg
• Active transport in cells usually occurs
with the help of carrier proteins but
REQUIRE energy. An example is the
sodium-potassium pump (Na+/K+ pump).
http://web.ahc.umn.edu/~mwd/cell_www/images/Na-Kpump.png
• When comparing 2 solutions with a membrane between them, there are 3 types of solutions:
• Hypertonic: the fluid outside a cell has a higher [solute] than the cytoplasm inside the cell. In this case, water diffuses out of the cell.
• Isotonic: the [solute] outside the cell= the [solute] inside the cell. In this case, no osmosis will occur.
• Hypotonic: the fluid outside a cell has a lower [solute] than the cytoplasm inside the cell. In this case, water will move inside the cell.
http://road-to-rn.tumblr.com/post/127238852030/hypotonic-
hypertonic-isotonic-solutions-videos
Bulk Transport:
1. Exocytosis:
exo=exit; cyto=cell
• Wastes and cell
products are
packaged in
vesicles by the
golgi apparatus.
• The vesicles fuse
with the cell
membrane and
leave the cell
http://www.octc.kctcs.edu/gcaplan/anat/images/Image152.gif
2. Endocytosis: endo=within; cyto=cell
• A portion of the cell membrane
surrounds a substance outside of the
cell & pinches off to form a vesicle
• The vesicle moves inward and fuses
with other organelles
• This includes
– Pinocytosis: cell drinking
– Phagocytosis: cell eating
https://www.youtube.com/watch?v=xuG4ZZ1GbzI
LIMITS TO CELL SIZE – view link for
SURFACE AREA TO VOLUME RATIO:
https://www.youtube.com/watch?v=URUJD5NEXC8&s
ns=em
DO VENN DIAGRAM after watching:
ENDOSYMBIOSIS Where did mitochondria, centrioles, and chloroplasts come from?
Evidence of Symbiosis
•Mitochondria and chloroplasts contain DNA, which is fairly different from that in the cell’s nucleus and that is similar to that of bacteria (circular and smaller). •They are surrounded by two or more membranes, and the innermost of these shows differences in composition compared to the other membranes in the cell. The composition is like that of a prokaryotic cell membrane. •New mitochondria and chloroplasts are formed only through a process similar to binary fission. •Much of the internal structure and biochemistry of chloroplasts, for instance the presence of thylakoids and particular chlorophylls, is very similar to that of cyanobacteria. •Centrioles are also believed to have developed by endosymbiosis. •The size of these organelles is comparable to bacteria. •These organelles' ribosomes are like those found in bacteria. •Most of this research began in the 1960’s by Lynn Margulis and was confirmed by later research in the 1980’s. (see animation attached )
http://highered.mcgraw-hill.com/sites/9834092339/student_view0/chapter4/animation_-_endosymbiosis.html
DOMAIN KINGDOM UNICELLULAR or
MULTICELLULAR
AUTOTROPH or
HETEROTROPH
PROKARYOTE or
EUKARYOTE
EUBACTERI
A or
BACTERIA
bacteria U both P
ARCHAEA archaebacteri
a
U both P
EUKARYA protists Mostly unicellular,
some multicellular
algae
both E
EUKARYA fungi Mostly multicellular;
yeasts: unicellular
H E
EUKARYA plants All M A E
EUKARYA animals ALL M H E
CLASSIFICATION - TAXONOMY