biology 22
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Biology 22. Drosophila Identification of various phenotypes. Outline. Brief description of Drosophila phenotypes and life cycle 1-Experiment 1: collection of data related to gender and body color 2-Experiment : 3 point Test Cross - PowerPoint PPT PresentationTRANSCRIPT
Biology 22
DrosophilaIdentification of various phenotypes.
Outline Brief description of Drosophila phenotypes and life cycle
1-Experiment 1: collection of data related to gender and body color
2-Experiment : 3 point Test Cross
How to study keep your flies still during the identification.
Reference for Pictures:
Carolina Drosophila melanogster Manual (45-2620)
Published: Carolina Biological Supply Company
Based on research of Dr. Thomas Hunt Morgan
1866-1945
The life Cycle
Life Cycle: Drosophila melanogaster
Eggs: contain filaments
2 molting phases “instars”
Life cycle is 10 days
Fruity Fly Egg and Larva
Fruit Fly Pupa and Adult
1- EXPERIMENT: Identification of:
Gender
Body Color
Wild Type Versus Mutants
Body Color
Wild type: BeigeMutant: Yellow body
Parental phenotypes Experiment: body color
A= female: wt (beige) B=female: wt (beige) C=male: wt (beige) D=female: mutant (yellow) E=male: mutant (yellow)
Fruit Fly Body Color
Sex combs: Male Drosophila
Female and Male Fruit Flies
Abdominal segements
7 segments to female abdominal segments
5 segments to male abdominal segments
Male body size is smaller than female
But the “last” abdominal segment is larger in male
2- Experiment: 3 point test cross:
Experiment 2 3 point test cross
Eye color Body color Wing length
3 point test cross for:
1. Body Color
2. Wing length
3. Eye Color
♦ Wt vs. Mutant
Beige (bl+) vs. Black (bl)
Long(vg+) vs. Vestigal(vg)
Brick red (br+) vs. brown (br)
Crosses:
Female Males
Males and female progeny from this F2 cross are your 3 point test cross results!
Xbl vg bw
bl vg bw
bl+ vg+ Bw+
bl vg bw
There are all sorts of eyes!
Eyes
You will have only red eyes (wt)
Fruit Fly Wings
Nap TimeTriethylamine
How to anesthetize a fly
How to use the Napper
View the Fruit Fly
You will record class data
After counting the crosses record the data on the class spreadsheet