BIOLOGICAL OXIDATION

Dr Vijay MarakalaAssistant professor

BiochemistrySIMS&RC

BIOLOGICAL OXIDATION

Oxidation Biological oxidation

Phosphorylation

Oxidative phosphorylatio

n

Biological oxidative

phosphorylation

Requirements for biological

oxidation

OXIDATION OF FOOD STUFFS IN THREE STAGES

Primary metabolism Secondary metabolism Tertiary metabolism

BIOLOGICAL OXIDATION

OXIDATION• Removal of electrons

REDUCTION• Gain of electrons

OIL

REG

BIOLOGICAL OXIDATION & PHOSPHORYLATION

Biological oxidationOxidation which occurs in living system

Phosphorylation The formation of ATP from

ADP and Pi

BIOLOGICAL OXIDATIVE PHOSPHORYLATION

Biol

ogic

al o

xida

tive

phos

phor

ylat

ionBiol

ogic

al

oxid

atio

n

Phos

phor

ylat

ion

BIOLOGICAL OXIDATION

REQUI

REM

ENTS

ENZY

MES

COEN

ZYM

ES

ELEC

TRON

CARR

IERS

BIOLOGICAL OXIDATION - REQUIREMENTS

ENZYMES

Oxidases

Dehydrogenases

Hydroperoxidases

Oxygenases

COENZYMES

NAD+

NADP+

FMN

FAD

BIOLOGICAL OXIDATION - REQUIREMENTS

CYTO

CHRO

MES

Cyt a

Cyt b

Cyt c

ELECTRON CARRIERS

OXIDASES

Cytochrome oxidase

L- amino acid oxidase

Xanthine oxidase

DEHYDROGENASES

Require specific coenzymes

Nicotinamide coenzymes• NAD+or NADP+

Flavin coenzymes• FMN or FAD

Coenzyme

Dehydrogenase specific for A

Dehydrogenase specific for B

NAD+ LINKED COENZYMES

Involved in

Glycolysis TCAETC

NAD+ NADH+H+

FLAVIN- COENZYME LINKED DEHYDROGENASE

2H

FMN FMNH2

HYDRO PEROXIDASES

CATALASESPEROXIDASES

DIOXYGENASESMONO OXYGENASES

OXYGENASES

ENZYMES N OF BIOLOGICAL OXIDATION

Use oxygen as an electron acceptorOxidases

Can’t use O2 as an electron acceptorDehydrogenases

Use H2O2 as a substrate

Hydroperoxidases

Catalyse the direct transfer of O2 into the substrate Oxygenases

ELECTRON CARRIERS

Iron

conta

inin

g

elect

ron

transfe

rring

prote

ins

CYTOCHROMES

Next class ETCPlease read and come