biochemistry grp 3 copy

BIOCHEMISTRYLABORATORY

LEADER: Kimberly Faye G. Fainiem

Group 3 : MT14206

SECRETARY: Ma. Jolina S. Ferrer

MEMBERS:Karen Escarez

Eunice ExpectacionVince LakapMariel Angeline Loyola

Pau Laxamana

LIPIDS

4 Categories of large molecules in cells:

CARBOHYDRATES

PROTEINS NUCLEIC ACIDS

Carbohydrates include:

Small sugar molecules in soft drinksLong starch molecules in pasta and

potatoes

CARBOHYDRATES

LIPIDS

Lipids:They are hydrophobic - “WATER FEARING”. Do NOT mix with water.

FAT MOLECULE

PROTEINS

Proteins includes: They are polymers made of

monomers called AMINO ACIDS.

All proteins are made of 20 different amino acids linked in different orders.

Proteins are used to build cells, act as hormones & enzymes, and do much of the work in the cell

NUCLEIC ACIDS

Nucleic Acids includes: Store hereditary information. Contains information for making all the body’s protein.

Two type exist: DNA RNA

EXPERIMENT 1

Experiment 1: Subcellular

ComponentsObjectives: To study the function of any organelle. To isolate it first to its pure form and free of

the contaminants by other organelles.

Experiment 1: Subcellular Components

Schematic DiagramProcedures

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2

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Data and Results

PRECIPITATE

NUCLEIC ACIDS

PROTEINS

LIPIDS

CARBOHYDRATES

I+++ ++ + +

II+ + +++ +++

III++ +++ ++ ++

NUCLEIC ACIDSDische Reaction (DNA) Diphenylamine (DPA) Reacts to deoxyribose of

DNA (+) blue color

ORCINOL TEST/BIALS

TEST (RNA)Specific test for pentoses (+) blue green color

PROTEINS

Biuret Test CuSO4 – oxidizing agent NaOH - reaction

medium Yields positive results

with polypeptides/ proteins but no with dipeptide

(+) violet color

LIPIDS

Sudan IV Hydrophobic dye 2 methyl phenylazo-beta-

naphtol (+) red color

CARBOHYDRATESMolisch Test Non-specific, general

test for CHO in free and combined form.

(+) purple/ violet color

RESULTS: Precipitate I

Carbohydrates

(-) purple color

Lipids

(-) red color

Proteins

(-) violet color

Nucleic Acids

(+) blue green color +++ ++ + +

RESULTS: Precipitate II

Carbohydrates

Lipids

Proteins

Nucleic Acids

(+) purple color

(+) red color

(-) violet color

(-) blue green color + + +++ +++

RESULTS: Precipitate III

Carbohydrates

Lipids

Proteins

Nucleic Acids

(+) purple color

(+) red color

(+) violet color

(+) blue green color ++ +++ ++ ++

EXPERIMENT 2

Experiment 2:

Cell Membrane (RBC)Objectives: To know the permeability of cell membrane. To know the osmotic pressure, presence of

ions present in the membrane.

Experiment 2: Cell Membrane


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biochemistry grp 3 copy

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