biochain next generation sequencing products
DESCRIPTION
Introducing Biochain Next Generation Sequencing ProductsTRANSCRIPT
Sample Preparation for Diagnostics & Life Science
A
G
C
T
Next Generation Sequencing Products
Next Generation Sequencing Products
CancerSeq-Characterized Cancer FFPE tissue samples by Next Generation Sequencing (NGS)
Sample and Services for Next Generation Sequencing
Cancer Gene Panel-NGS Testing Service
RapidSeq-Directonal mRNA Sample Prep Kit for NGS
RapidSeq-Small RNA Sample Prep Kit for NGS
Sample Preparation for Diagnostics & Life Science
www.biochain.com
1-888-762-2568 (Main)
[email protected] (Order)
BioChain’s CancerSeq™ genetically characterized FFPE blocks give you and your data more power
Blocks and Slides available for:
- Breast Cancers - Colon Cancers - Lung Cancers - Melanomas - Other Cancers (via request)*
Cancer Gene Panel Includes:
The Illumina TruSeq® Amplicon - Cancer Panel (TSACP) to characterize 48 cancer genes, including BRAF, KRAS, EGFR, ALK, and Tp53.
Features- Tissue from a wide variety of sources, Whole FFPE blocks, slides, and DNA
- Suitable for immunohistochemistry, in situ hybridization assays,
and molecular applications
ABL1 ERBB4 JAK2 PIK3CAAKT1 FBXW7 JAK3 PTENALK FGFR1 KDR PTPN11APC FGFR2 KIT RB1ATM FGFR3 KRAS RETBRAF FLT3 MET SMAD4CDH1 GNA11 MLH1 SMARCB1CDKN2A GNAQ MPL SMOCSF1R GNAS NOTCH1 SRCCTNNB1 HNF1A NPM1 STK11EGFR HRAS NRAS TP53ERBB2 IDH1 PDGFRA VHL
CATALOG # PRODUCT NAME SIZET2235086-SB CancerSeq™ Paraffin Tissue Tumor Block: Breast 1 BlockT2235090-SB CancerSeq™ Paraffin Tissue Tumor Block: Colon 1 BlockT2235152-SB CancerSeq™ Paraffin Tissue Tumor Block: Lung 1 BlockT2235218A-SB CancerSeq™ Paraffin Tissue Tumor Block: Skin Melanoma 1 Block
- Make sense out of ambiguous IHC results by knowing the genetics- Buy just the blocks with the right genetic background - Search our databases for blocks with novel mutations - Take advantage of NGS without expensive infrastructure
BioChain’s CancerSeq™ genetically characterized FFPE blocks give you and your data more power
NGS Samples and Services
As a provider of tissues and nucleic acids derived from them, BioChain is uniquely positioned to extract DNA and RNA from the most challenging tissues, and perform re-sequencing and RNAseq.
Tissues: We have over 300,000 Frozen and FFPE blocks to collect DNA and RNA
from. You may also contract with us to procure rare materials for your sequencing project.
Extraction: We have provided high quality DNA and RNA from the most challenging
tissues including FFPE blocks, cartilage, and brain for over 15 years.
Library preparation: We are Experienced with all Illumina library preparation kits as well as
our proprietary RapidSeqTM small RNA library prep kit.
Illumina MiSeq NGS: With special expertise in RNAseq and resequencing with Trueseq
Custom and Cancer Amplicon panels, we handle a wide variety of sequencing projects.
Data analysis and consultation: We can help with experimental design through variant calling and anno-
tation.
Cancer Driver Gene PanelNGS Testing ServiceOne - Step Shop Cancer Sequencing Test Services from Beginning to End
NGS Leading Platform- - Greater than 35 kb of cancer consensus targets including BRAF, KRAS & EGFR - 212 amplicons in one tube; 48 genes
Unrivaled Multiplexing
- Up to 96 sample pooling on MiSeq - >90% specificity and uniformity- Detect low frequency variants (<5%)
Unparalleled Workflow
- FFPE-enabled with sample QC Kit- Automated paired end sequencing with MiSeq
Industry Standard Delivery
- Cost-effective solution- Quick turn-around time
ABL1 EGFR GNAS MLH1 RETAKT1 ERBB2 HNF1A MPL SMAD4ALK ERBB4 HRAS NOTCH1 SMARCB1APC FBXW7 IDH1 NPM1 SMOATM FGFR1 JAK2 NRAS SRCBRAF FGFR2 JAK3 PDGFRA STK11CDH1 FGFR3 KDR PIK3CA TP53
CDKN2A FLT3 KIT PTEN VHLCSF1R GNA11 KRAS PTPN11
CTNNB1 GNAQ MET RB1
48 Cancer Biomarker
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACCCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGG
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACCCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGAATTCCGGCCGATCAGGGGTTTACACCAATG
GGTTTACACCAATGGGACCATTACCAAC
Directional mRNA Sample Prep Kit
Sample Prep System for mRNA Sequencing
Features
• Low RNA Input Requires as little as 50 ng of total RNA input• Directional Increased useful number of reads• < 1 Hour Hands-on Time Streamlined workflow Library construction in < 7 hours• All-in-One Kit Includes all necessary enzymes and plastics • FFPE RNA Compatible
High Quality Libraries withoutArtifacts or Contaminations
Bioanalyzer Trace of Constructed Library of mRNA from corn
DescriptionThe RapidSeq Directional mRNA Sample Prep kit provides strand-specific cDNA synthesis
with reduced costs and increased sensitivity.
Directionality Assessment
Comparison with TruSeq in Prokaryotic Cells
mRNA Input (ng) Sample Type % Sense
100 (Competitor I Kit) FFPE mRNA (1 year) 97.85%100 (RapidSeq Kit) FFPE mRNA (1 year) 97.88%500 (Competitor I Kit) FFPE mRNA (10 years) 97.59%500 (RapidSeq Kit) FFPE mRNA (10 years) 97.57%
Parameter TruSeq RapidSeq Coverage Rate 44.00% 65.00%
rRNA Contamination with 23s, 16s, and 5s removal
2.70% 1.50%
tRNA contamination 64.00% 57.00%Unknown sequence percentage 5.00% <1% Directional Percentage 74% 77.50%Average Quality Score (range from 0 to 40, with 40 being the highest quality)
26 37
CATALOG # PRODUCT NAMEKS073012 RapidSeq High Yield Directional mRNA Sample Prep Kit - Without Aligner
KS073012 I-IV RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligners 1-48
K2012008 MagSeq mRNA Puri�cation Kit
AATTCCGGCCGATCAGGGGTTTACACCAATG
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACCCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGGTGGCCATGGAACCTTGGACCCA
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACCCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGGTGGCCATGGAACCTTGGACCCATTTAGAACAG
Small RNA Sample Prep Kit Everything you need in one box
Applications
• Small RNA discovery and quantification• miRNA expression profiling• miRNA related functional assessments
DescriptionThe RapidSeq Small RNA Sample Prep Kit
is a cost effective and reliable solution for small RNA library preparation using Illumina’s sequencing platform. Our reagents are master mixed for optimal performance and simple experiment setup, which minimizes human error. RapidSeq can be used for a variety of studies involving small RNA libraries.
Construct Pure Libraries with no Artifacts or Contamination
Bioanalyzer Trace of FFPE microRNA Library
Highly Consistent andReproducible Results
Normalized R2 Plot of miRNA counts from Two Replicate Libraries
CATALOG # PRODUCT NAMEKS071012-I RapidSeq Small RNA Sample Prep Kit - With Aligner 1-12KS071012-II RapidSeq Small RNA Sample Prep Kit - With Aligner 13-24KS071012-III RapidSeq Small RNA Sample Prep Kit - With Aligner 25-36KS071012-IV RapidSeq Small RNA Sample Prep Kit - With Aligner 37-48
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACCCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGGTGGCCATGGAACCTTGGACCCA
Save time Less than 1 hour hands-on (7 hours with incubations).
Avoid purchasing headaches Includes all necessary enzymes and plastics.
Don’t worry about RNA quantity Good results with as little as 1 ng or as much as 10 µg of total RNA.
Have FFPE RNA? No problem! FFPE RNA compatible
NGS Related Products
T2235086-SB CancerSeq Parafin Tissue Tumor Block: Breast 1 BlockT2235090-SB CancerSeq Parafin Tissue Tumor Block: Colon 1 BlockT2235152-SB CancerSeq Parafin Tissue Tumor Block: Lung 1 BlockT2235218A-SB CancerSeq Parafin Tissue Tumor Block: Skin Melanoma 1 Block
KS073012-I RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 1-12 1 KitKS073012-II RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 13-24 1 KitKS073012-III RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 25-36 1 KitKS073012-IV RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 37-48 1 KitKS073012 RapidSeq High Yield Directional mRNA Sample Prep Kit - Without Aligner 1 Kit
KS073012-T RapidSeq High Yield Directional mRNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners
1 Kit
KS074012-I RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 1-12 1 KitKS074012-II RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 13-24 1 KitKS074012-III RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 25-36 1 KitKS074012-IV RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 37-48 1 KitKS074012 RapidSeq High Yield Small RNA Sample Prep Kit - Without Aligners 1 Kit
KS074012-T RapidSeq High Yield Small RNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners
1 Kit
KS075012-I RapidSeq MasterMix Directional mRNA Sample Prep Kit - With Aligner 1-12 1 KitKS075012-II RapidSeq MasterMix Directional mRNA Sample Prep Kit - With Aligner 13-24 1 KitKS075012-III RapidSeq MasterMix Directional mRNA Sample Prep Kit - With Aligner 25-36 1 KitKS075012-IV RapidSeq MasterMix Directional mRNA Sample Prep Kit - With Aligner 37-48 1 KitKS075012 RapidSeq Mastermix Directional mRNA Sample Prep Kit - Without Aligners 1 kitKS071012-I RapidSeq MasterMix Small RNA Sample Prep Kit - With Aligner 1-12 1 KitKS071012-II RapidSeq MasterMix Small RNA Sample Prep Kit - With Aligner 13-24 1 KitKS071012-III RapidSeq MasterMix Small RNA Sample Prep Kit - With Aligner 25-36 1 KitKS071012-IV RapidSeq MasterMix Small RNA Sample Prep Kit - With Aligner 37-48 1 KitKS071012 RapidSeq MasterMix Small RNA Sample Prep Kit - Without Aligner 1 Kit
CATALOG # DESCRIPTION UNITS
Other Related Products
D1B34999-G02 Control Genomic DNA - Bovine Female 100 µgD1B34999-G01 Control Genomic DNA - Bovine Male 100 µgD1C34999-G02 Control Genomic DNA - Chicken Female 100 µgD1C34999-G01 Control Genomic DNA - Chicken Male 100 µgD1534999-Cy-G02 Control Genomic DNA - Cynomolgus Monkey Female 100 µgD1534999-Cy-G01 Control Genomic DNA - Cynomolgus Monkey Male 100 µgD1734999-G02 Control Genomic DNA - Dog Female 100 µgD1734999-G01 Control Genomic DNA - Dog Male 100 µgD1G34999-G02 Control Genomic DNA - Guinea Pig Female 100 µgD1G34999-G01 Control Genomic DNA - Guinea Pig Male 100 µgD1H34999-G02 Control Genomic DNA - Hamster Female 100 µgD1H34999-G01 Control Genomic DNA - Hamster Male 100 µgD1234999-G02 Control Genomic DNA - Human Female 100 µgD1234999-G01 Control Genomic DNA - Human Male 100 µgD1334999-G02 Control Genomic DNA - Mouse Female 100 µgD1334999-G01 Control Genomic DNA - Mouse Male 100 µgD1934999-G02 Control Genomic DNA - Porcine Female 100 µgD1934999-G01 Control Genomic DNA - Porcine Male 100 µgD1834999-G02 Control Genomic DNA - Rabbit Female 100 µgD1834999-G01 Control Genomic DNA - Rabbit Male 100 µgD1434999-G02 Control Genomic DNA - Rat Female 100 µgD1434999-G01 Control Genomic DNA - Rat Male 100 µgD1534999-G02 Control Genomic DNA - Rhesus Monkey Female 100 µgD1534999-G01 Control Genomic DNA - Rhesus Monkey Male 100 µg
R1234010-50 Total RNA - Human Adult Normal Tissue: Bladder 50 µgR1234035-50 Total RNA - Human Adult Normal Tissue: Brain 50 µgR1234086-50 Total RNA - Human Adult Normal Tissue: Breast 50 µgR1234090-50 Total RNA - Human Adult Normal Tissue: Colon 50 µgR1234090-P Total RNA - Human Adult Normal Tissue 5 Donor Pool: Colon 50 µgR1234122-50 Total RNA - Human Adult Normal Tissue: Heart 50 µgR1234142-50 Total RNA - Human Adult Normal Tissue: Kidney 50 µgR1235010-10 Total RNA - Human Tumor Tissue: Bladder 10 µgR1235023-10 Total RNA - Human Tumor Tissue: Bone 10 µgR1235086-50 Total RNA - Human Tumor Tissue: Breast 50 µgR1235090-50 Total RNA - Human Tumor Tissue: Colon 50 µgR1235142-50 Total RNA - Human Tumor Tissue: Kidney 50 µgR1235149-50 Total RNA - Human Tumor Tissue: Liver 50 µg
Control Genomic DNA
Total RNA
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACCCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGG
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACCCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGGTGGCCATGGAACCTTGGACCCATTTAGAACAG
Sample Preparation for Diagnostics & Life Science
www.biochain.com
1-888-762-2568 (Main)
[email protected] (Order)