biobuilder eau d’coli biobuilder educational foundation natalie kuldell
TRANSCRIPT
BioBuilder
Eau d’coli
BioBuilder Educational Foundation
Natalie Kuldell
System level description (initial)
Black box functionsInput Output
System level description (initial)
Black box functionsPrecursor
compound Smell
Device level description (initial)
Precursor compound Smell
WGD: Wintergreen Generating Device
WGD
BGDand
BGD: Banana Generating Device
Device List !!
System level description (revised)
Cell Growth SmellWGD
andWGD
BGD
Device level description (revised)
Cell Growth Smell
WGD: Wintergreen Generating Device
WGD
BGDand
BGD: Banana Generating Device
saGD
iaGD
saGD: salicylic acid Gen Dev iaGD: isoamyl alcohol Gen Dev
Device List !!
Parts specification for stationary phase banana smell
BGDcell density
time
“lag”
“log”
“stationary”
OsmY is a promoter that is only active during stationary phase
Parts specification for log phase wintergreen smell
WGD
cell density
time
“lag”
“log”
“stationary”
Stationary LogOsmY 1 0OsmY + inverter
0 1
One last modification to the E. coli chassis
cell density
time
“lag”
“log”
“stationary”
eliminate natural indole production
saGD
iaGD BGD
WGD
“Eau d’coli”
cell density
time
“lag”
“log”
“stationary”
saGD
iaGD BGD
WGD