bio degradation of pollutants
DESCRIPTION
Para sa last exam sa BC 168TRANSCRIPT
Transformation and Degradation of Pollutants
Important Variables for Contaminant Transformations
1. Chemical Structure of the Contaminant
2. Presence of Transforming Species
3. Physical Availability of the contaminant.
4. Dissolved Oxygen
5. pH
6. Temperature
Abiotic Transformation of Pollutants
1. Nucleophilic Substitution
2. Elimination Reaction
3. Oxidation/Reduction
OH = One of the most important oxidants found in air, water and biological systems.
Sources of OH Radical:
a. Fenton Reaction
b. Reaction of Ozone wigth NOx
Biodegradation Reactions and Pathways of Hazardous Contaminants
Microbial Transformation of Pollutants
Microorganisms involved in Biotransformations
1. Bacteria
2. Fungi
3. Algae
4. Protozoan
Classification of Bacteria in Microbial Metabolism
1. Based on Energy Sourcesa. Chemotrophsb. Phototrophs2. Based on Sources of Carbona. Autotrophsb. Heterotrophs3. Based on Sources of Electronsa. Organotrophsb. Lithotrophs
Microbial Growth Pattern
Lag PhaseLog Phase
Stationary Phase
Death Phase
Mic
robi
al P
opul
atio
n
Time
Conceptual Basis for Biodegradation Reaction
Contaminant + Xox Product + Xred
Redox Couple
» Eo’ (V)
• Succinate + CO2 + 2H + 2e a-ketoglu + H2O -0.67
• AcetylcoA + CO2 + 2H + 2e Pyruvate + COAsH -0.48
• A-ketoglu + CO2 + 2H + 2e isocitrate -0.38
• Acetaldehyde + 2H + 2e ethanol -0.20
• Pyruvate + 2H + 2e Lactate -0.19
• Oxaloacetate + 2H + 2e Malate -0.17
• Fumarate + 2H + 2e Succinate 0.03
Substrate Redox Couple
ETS Couple (Aerobic Respiration
2H + 2e - H2 -0.42
Ferridoxin (Fe3+) + 1e Ferridoxin (Fe2+) -0.42
NAD + H+ + 2e --- NADH -0.32
NADP + H+ + 2e - NADPH -0.32
FAD + 2H + 2e - FADH2 -0.18
Cytb(Fe2+) + 1e Cytb(Fe2+) +0.10
Cytc (Fe3+) + 13 Cytc (Fe2+) 0.25
Cyta3 (Fe3+) + 1e Cyta3 (Fe2+) 0.55
O2 + 4H +4e - 2H2O 0.82
• 2H + 2e - H2 -0.42• Ferridoxin (Fe3+) + 1e Ferridoxin (Fe2+) -0.42• NAD + H+ + 2e --- NADH -0.32• NADP + H+ + 2e - NADPH -0.32• FAD + 2H + 2e - FADH2 -0.18• Cytb(Fe2+) + 1e Cytb(Fe2+) 0.10• Cytc (Fe3+) + 1e Cytc (Fe2+) 0.25• Cyta3 (Fe3+) + 1e Cyta3 (Fe2+) 0.55• O2 + 4H +4e - 2H2O 0.82
ETS Couple (Aerobic Respiration
ETS Couple (Anaerobic Respiration
SO42- + 3H + 2e - HSO3- + H2O -0.52
NO3- +2H + 2e NO2- + H2O +0.42
NO2- + 8H + 6e NH4
+ + 2H2O +0.44
Fe3+ + 1e - Fe2+ +0.77
Dependence of Bacteria on Electron Acceptor
• Aerobic Reaction- O2 is the e- acceptor
• Anaerobic Process: 3 Pathways• 1. Anaerobic Respiration• 2. Fermentation• 3. Methanogenesis
Aerobic Metabolism Pathways
• Emden-Meyerhof Pathway• TCA• Electron Transport System• Glucose Metabolism:• Glucose Pyruvate 2 ATP and 2 NADH• 2 Pyruvate 2 AcetyCoA 2 NADH
• 2 AcetylcoA 4 CO2 + 2 H2O 6 NADH, 2 FADH, 2 ATP
• Lactate Fermentation• Glucose + 2 NAD+ + 2 ADP + 2Pi 2 Pyruvate
+ 2 NADH + 2 H+ + 2 ATP• 2 NADH + 2 H + 2 Pyruvate 2 Lactate• Glucose + 2 ADP + 2Pi 2 Lactate + 2 ATP
Ethanol Fermentation
Glucose + 2 NAD+ + 2 ADP + 2Pi 2 Pyruvate + 2 NADH + 2 H + 2 ATP
2 Pyruvate - 2 Acetaldehyde + 2 CO2
2 Acetaldehyde + 2 NADH + 2 H - 2 Ethanol + 2 NAD+
Glucose + 2 ADP + 2 Pi - 2 Ethanol + 2 CO2 + 2 ATP
Methanogenesis:
CH3COOH -- CH4 + CO2
CO2 + 4 H2 - CH4 + 2 H2O
• Important Bacteria in Hazardous Waste Systems:
• White Rot Fungus or Wood Rot Fungus
Examples:
Phanerochaete Chrysosporum
Phanerochaete sordida
-These fungi have been shown to degrade PAH, PCB,
pentachlorophenol, DDT through the activity of extracellulase peroxidase enzyme
Important Bacteria in Hazardous Waste Systems:
a. Pseudomonas
b. Nocardia
c. Mycobacterium
d. Arthrobacteria
e. Bacillus
Biodegradation Reactions and Pathways of Hazardous Contaminants
• 1. Contaminants pass through the cell membrane.• 2. Compounds too large to pass through the cell
membrane may be partially degradaded by exoenzymes which are secreted through the cell wall.
• 3.The following reactions will occur in the cytoplasm: Hydroxylation, Hydrolysis, Dehalogenation, Dealkylation and Reduction
• 4. Dehydrohalogenation
Oxidative Processes for which Oxygen is the Electron Acceptor
• Monooxygenases are characterized by their ability to introduce one of the atoms of O2 into an organic substrate, S and the other being incorporated into a molecule of water.
• S + O2 + AH + H+ -- SO + A + H2O
• Reactions catalyzed by Monooxygenases:• 1. Hydroxylation at Saturated and Unsaturated Carbon• 2. Epoxidation of Olefin• 3. Baeyer-Villiger Oxidation of Ketones• 4. Oxidation at S and N• 5. Heteroatom Dealkylation
Protoporphyrin Ring
N
NN
N
CO2-
CO2-
Fe2+
S(Cys-Protein)
Fe3+
Fe2+---S
S---3+Fe
OOH
S
4+Fe=O
S, 1e
O2, e, H+H+
H2O
S(O)
Fe2+P + O2 -----> Fe3+ (O2-) -----> Fe3+(O2
-) -------> Fe3+(OOH)
Fe3+(OOH)----> Fe4+(O2-) + HO
Fe3+(OOH)---->Fe4+P(O2-) + HO-
HomolyticBond Cleavage
HeterolyticBond Cleavage
1e H+
.
• Cytochrome P450-Dependent monooxygenase delivers oxygen to the substrate in the form of a heme-iron oxo complex
• Responsible for the majority of biological hydroxylation, epoxidation, and heteroatom dealkylation
Hydroxylation of Alkane
Fe3+P(OOH) + CH4 Fe4+P(O2-) + CH3
. +H2O
Fe4+P(O2-) + CH3
. ---> Fe3+P-OCH3 --
[Fe3+P] + HOCH3
Epoxidation of Alkene
Fe3+P(OOH) + CH2=CH2 Fe3+P(OH) + epoxide
O
OH O
Hydroxylation of Benzylic Carbon, Amines and Mercaptans
-Hydroxylation of the substrate can occur following the removal of one electron from the aromatic ring.
R Fe3+P(OOH)+.
R R
.R
HO
Fe4+P(O-2) + HO.
Fe4+PO. + CH3NH2 ----> Fe4+PO- + CH3NH2+. ----> CH2=NH2
+
PFe3+ + CH3-N+-O- Fe3+PO-N(CH3)3+
Fe4+O. + -S- ------> Fe4+-O- + -S+-
-S-
OFe3+ +
Fe4+P(O2-)EnzymeRecycling
EnzymeRecycling
Initial Degradation of Benzene