Big Sandy Community and Technical College

Course Syllabus

PS Number: Lecture: 48780 Lab: 48781

Semester: Fall Year: 2017

Faculty Name: Clinton Hackney Title: Assistant Professor

Course Prefix and Number: BIO 225 Course Credit Hours: 4

Course Prerequisites: BIO 137 & BIO 139 or equivalent.

Course Title: Medical Microbiology

Catalog Course Description: The characteristics of microorganisms and their relation to health and disease are studied. Lecture: 2 credits (30 contact hours); Laboratory: 2 credit (60 contact hours)

Instructor Contact Information:

Campus Location: Pikeville Building & Room: Clayton N. Little #327N

Office Hours: Posted to Office Door and to Blackboard

Office Phone Number: 606-218-1268 Alternate Number: 606-889-1268

Best Times to Call: 10am-3pm

KCTCS Email: Chackney0005@kctcs.edu

Special Instructions: Email is the desired contact method

Supervisor Contact Information:

Name: Dr. Lisa Music, Division Chair Math and Sciences

Campus Location: Prestonsburg Building & Room: Pike Building 107F

Office Phone Number: 606-889-4760

KCTCS Email: Lisa.music@kctcs.edu

Text and Supplies:

Fundamentals of Microbiology 8th Edition, Kathleen Park Talaro or newer. Pollack, Findlay, Mondschein, and Modesto Laboratory Exercises in Microbiology 4th Edition.

KCTCS General Education Competencies

Students should prepare for twenty-first century challenges by gaining: A. Knowledge of human cultures and the physical and natural worlds through study in the sciences

and mathematics, social sciences, humanities, histories, languages, and the arts. Method to Achieve Competency: Written Research Paper Graded on a Rubric IAW KCTCS competencies.

B. Intellectual and practical skills, including

• inquiry and analysis

• critical and creative thinking

• written and oral communication

• quantitative literacy

• information literacy

• teamwork and problem solving Method to Achieve Competency: Measure through lab activities and evaluated through lab practical.

C. Personal and social responsibility, including

• civic knowledge and engagement (local and global)

• intercultural knowledge and competence

• ethical reasoning and action

• foundations and skills for lifelong learning Method to Achieve Competency: Power point presentation over assigned physiological topic.

D. Integrative and applied learning, including synthesis and advanced accomplishment across general and specialized skills.

Method to Achieve Competency: Lecture and Lab.

Course Specific Competencies (Student Outcomes):

Students achieving a passing grade will be able to demonstrate proficiency in the following areas, to a degree commensurate with the grade received. 1. Review history of Microbiology with key contributors 2. Identify key technological advances/discoveries in Microbiology 3. Associate diseases with symptomologies and specific disease-causing microbes 4. Understand basic lab protocols to screen for presence of specific microbes 5. Study epidemiology’s of infectious diseases

Lab Competencies (Student Outcomes): (Enter N/A if this does not apply.)

Students achieving a passing grade will be able to demonstrate proficiency in the following areas, to a degree commensurate with the grade received. 1. Understand the basic applications of laboratory protocol and safety under OSHA guidelines 2. Become proficient in the use of a compound light microscope utilizing the highest magnification (1000x) to view microbes. 3. Identify microorganisms by morphology 4. Prepare differential and selective media 5. Apply staining techniques and study antibiotic effects of specific microbes 6. Produce quality lab reports and maintain a laboratory notebook

Course Outline:

Lecture Topics Central Dogma, Microbiological Historical Perspectives, Prokaryotic & Eukaryotic Structure and Function, Tools of Laboratory Topics (Chapters 1, 2, 3 & 4)

Lecture Exam I DNA and RNA Viruses (Chapters 6 & 7)

Lecture Exam II Microbial Nutrition and Growth, Antibiotics and Antimicrobial Therapy, Microbe/ Human Interactions (Chapters 8, 9, 10 & 11) Adaptive and Specific Immunity, Parasitology, Cocci of Medical Importance, Gram (+) and Gram (-) Bacilli of Medical Importance (Chapters 12, 13, 14, 15, 16 & 17)

Lecture Exam III / Final Exam *Dates for exams will be announced as we approach completion of a block of instruction. **Although exams are not comprehensive, understanding of concepts from each block of instruction is vital for success on subsequent exams.

Course Structure:

Lecture: Lecture Material will be presented in powerpoint, chalkboard, and transparency format. Students are highy encouraged to print off powerpoint notes prior to coming to class in order to annotate them with instructors comments. The lecture portion of the course will constitute 65% of the total grade assigned. Within the scope of the 65%, the following items will be incorporated: Lecture Exams (3) = 50% of total grade Lecture Exams will be announced and consist of definition, essay, multiple choice, short answer, and /or matching questions each with assigned point values to equal 100 points total. No mobile devices may be turned on during any lecture or lab exams. Quizzes (Top 10 scores) = 10% of total grade Lecture quizzes will be unannounced and typically consist of material covered in the previous lecture. Lecture quizzes may be a combination of definition , multiple choice, short answer, and /or matching

questions and will each be worth 10 points (cumulatively standardized to 10% of total grade). If given, quizzes will be handed out at the beginning of each lecture meeting and students will be given 5-10 minutes to complete. Group presentations (1) = 5% of total grade Students will be graded individually on the group presentation. Group presentations will consist of both a written and oral component delivered through power point. Further details regarding presentations will be given by the instructor and posted to blackboard.

Laboratory: The laboratory portion of the course will constitute 35% of the grade assigned. Within the scope of the 35%, the following items will be incorporated: Laboratory Practical Exams (1) = 10% of total grade Lab exams will consist of a written and practical component. (See Addendum A for Lab Outline) Laboratory Reports / Identification of Unknown (13) = 25% of total grade Lab reports will consists of hand-on activities and will be turned in no later than one week upon completion of a lab. Identification of Uknown will count as a lab report and demonstrate proficiency in concepts discussed thorought the semester.

Technology/Media Component:

Instructional delivery will be enhanced by the use of transparencies, powerpoint presentations, and online notes available through blackboard. Information will be supplied to the students on blackboard log-in / downloading and anatomy and physiology-related websites. Students will be encouraged to utilize the internet to obtain peer-reviewed journal articles and check student email/blackboard for announcements, notes, etc.

Service-Learning: These activities are not applicable to this course.

Course Requirements and Evaluation:

The lecture portion of the course will constitute 65% of the total grade from which 3 tests (16.67% of the total grade each), unannounced quizzes (cumulatively 10% of the total grade), and one group paper with powerpoint (5% of total grade) will be used for evaluation. The laboratory portion of the course will constitute 35% of the total grade from which 1 laboratory practical exam (10% of the total grade each) and lab exercises (25% of the total grade) will be used for evaluation. Lecture Quizzes: Although these will be unannounced, we will take 12-15 over the course of the semester. From all the quizzes, only the student’s top 10 scores will be used to determine the lecture quiz portion of the overall grade. No make-up quizzes will be given for any reason other than simple study guides (i.e. no points for tardiness or absence) Group Presentations: Groups will choose a topic to present to the class / instructor in both oral (i.e. power point, blackboard, transparency, etc.) and written form. Students will be evaluated individually on performance. No make-ups will be given if a student misses their presentation date. The written portion of the presentation will be graded in accordance with approved KCTCS competencies and rubric built into blackboard. Extra Credit: Up to 5% extra credit will be given to any student who wishes to write a 5-page research paper on an approved topic within Anatomy and Physiology. Topics must be submitted to and approved by the instructor no later than 24 October 2017. Additional information on the paper's format will be handed out.

Grading Policy:

A = 90-100% of total grade B= 80-89% of total grade C= 70-79% of total grade D= 60-69% of total grade E= Below 60% of total grade

Attendance Policy:

A specific attendance policy does not exist for this course. However, lecture and laboratory examinations cannot be made up unless the excuse for absence is determined to be valid and supporting documentation is presented. This includes, but is not limited to illness, serious illness/death of family member(s), and other unforeseen circumstances. No make-ups will be administered for lecture quizzes. *Cell phones and other mobile devices must be turned off or silenced during lecture. If your device goes off at any time you will be asked to leave. Food and drink are not permitted in lab or lecture areas.

Missed Exam Policy:

Due to time and resource constraint of laboratory exams, they cannot be made up under any circumstance. Lecture exams may be made up no later than one week past the exam date if the excuse is determined valid and documentation is presented. Exams will be done in my office suite at a scheduled time and may be reformatted from the original exam.

Late Assignment Policy:

Extra Credit assignments may not be made up and must be received and approved before 24 October 2017

Withdrawal Policy:

A student may withdraw through the final withdrawal date officially designated for the semester and receive a "W" with consent of instructor.

Additional information can be found at the following site: http://www.bigsandy.kctcs.edu/Academics/Office_Hours_Schedules_and_Syllabi.aspx

Addendum A BIO 225 Fall 2017 Tentative Lab Schedule

All Exercises will either come from the instructor or from Pollack, Findlay, Mondschein, and Modesto Laboratory Exercises in Microbiology 4th Edition. A copy of the lab manual has been put on reserve in the library for student access. Week 1: *Lab Safety Rules and Introduction to Microscopy *Objectives include: **Aseptic Technique (hand washing, bench cleaning, etc.) **OSHA Guidelines, “Right to Know” Act *Monday: Introduction to lab equipment and safety rules *Wednesday: Microscopy – refresher on how to use a compound light microscope *Assignment: Complete lab report 1 and turn in for grading Week 2: *Transfer and Isolation Techniques, Microbes in the Environment *Objectives include: **Transfer bacteria aspectically between tubes of growth media **Aspectically perform a streak plate resulting in isolated colonies **Properly prepare a pour plate **Determine ubiquitous nature of microorganisms *Monday: Transfer and inoculate media *Wednesday: Inspect tubes and plates *Assignment: Complete lab report 2 and turn in for grading Week 3: *Microbial Morphology (Cultural and Cellular) *Objectives include: **Properly prepare bacterial smears from broth and from agar for staining **Distinguish between different bacterial morphologies on agar plates and slants **Recognize the following morphological shapes and arrangements: ***diplococci ***streptococci ***staphylococci ***single rods (bacillus) ***spirilla *Monday: Inoculate plates and tubes *Wednesday: Stain *Assignment: Complete lab report 3 and turn in for grading

Week 4: *Microbial Growth *Objectives include: **Explain why growth in bacteria is different from growth in humans **Identify the components of a bacterial growth curve **Determine viable cell numbers in a culture tube by performing a pour plate technique **Quantitate bacterial numbers and determine a growth curve *Monday: Inoculate bacteria/dilutions *Wednesday: Read and analyze results *Assignment: Complete lab report 4 and turn in for grading Week 5 *Gram Stain and Acid-Fast Stain *Objectives include: **Describe the principal of a differential stain **Properly perform a Gram stain **Differentiate between Gram positive, Gram negative, and Gram variable reactions **Properly perform and acid-fast stain **Differentiate between and acid-fast and nonacid-fast staining reactions

*Monday: Inoculate media *Wednesday: Perform differential stains Complete lab report 5 and turn in for grading Week 6: *Endospore Stain, Capsule Stain, Hanging Drop Technique *Objectives include: **Properly preform and endospore stain **Differentiate between endospore formation and nonendospore formation **Recognize the presence of bacterial capsules under the microscope **Properly preform a hanging drop technique and assess for motility

*Monday: Prepare and inoculate microbes *Wednesday: Perform stains *Assignment Complete lab report 6 and turn in for grading Week 7: *Microbial Sensitivity Lab I (Virtual) *Objectives include: **Determine advantages and disadvantages of using UV light as a sterilizing agent **Determine how evidence of mutations can be detected when a microbe is subjected to UV light **Determine whether some species of bacteria can resist high temperature while others cannot, and formulate answers as to why.

*Monday: Prepare bacteria and discuss microbial sensitivity *Wednesday: Conduct sensitivity experimentation *Assignment Complete lab report 7 and turn in for grading

Week 8: *Microbial Sensitivity Lab II (Hands on) *Objectives include: **Determine which species of bacteria were better able to withstand UV light, and formulation answers to why. **Relate the principle of Kirby-Bauer method of antibiotic sensitivity **Determine what constitutes a susceptible or sensitive reaction, and intermediate reaction, and a resistant reaction with the Kirby-Bauer method of determining antibiotic resistance.

*Monday: Inoculate Kirby-Bauer plates with antibiotic discs *Wednesday: Read plates and perform calculations

*Assignment Complete lab report 8 and turn in for grading Week 9: *Fungi and Viruses *Objectives include: **Properly differentiate between the four major divisions of fungi **Identify sporangiophores, sporangiospores, and sporangia **Identify zygospores **Identify conidiophores and conidia **Differentiate between septate and nonseptate hyphae **Identify a mycelium, yeast cells, and budding **Describe what viruses are **Differentiate between various structural forms of viruses **Prepare a serial dilution **Identify and count a bacteriophage plaque **Determine the number of bacteriophages in a suspension

*Monday: Fungi *Wednesday: Viruses *Assignment Complete lab report 9 and turn in for grading Week 10: *Bacterial Biochemistry *Objectives include: **Explain the concept of one enzyme, one substrate, one reaction **Explain why other microbes thrive in environments that are detrimental to other life **Determine how to recognize whether a microbe possesses enzymes to: ***ferment glucose and/or lactose and produce acid or gas byproduct ***oxidize glucose ***Utilize citrate as a source of high energy carbon ***Metabolize amino-acid tryptophan into indole ***Remove carboxyl group from amino acid ***Reduce nitrates into nitrites and nitrogen gas ***Catabolize urea into ammonia by hydrolysis

*Monday: Inoculate tubes *Wednesday: Read reactions in tubes *Assignment Complete lab report 10 and turn in for grading Week 11: *Gas Requirements of Microorganisms *Objectives include: **Differentiate microbes according to their gas requirements **Explain the various ways to grow anaerobic bacteria **Explain why there is bubbling when hydrogen peroxide is placed on a wound

*Monday: Inoculate media *Wednesday: Read results *Assignment Complete lab report 11 and turn in for grading Week 12: *Specialized Media *Objectives include: **Explain the function of enriched, selective, highly selective, differential, transport, and multitest media. **Differentiate between alpha, beta, and gamma hemolysis on a BAP **Describe the functions of MacConkey (MAC) and eosin-methylene blue (EMB) agar **Explain the function of mannitol salt agar (MSA) **Explain the function of phenylethyl alcohol agar (PEA) **Describe the possible reactions of triple sugar iron agar (TSIA) and sulfide-indole motility medium (SIM)

*Monday: Inoculate media *Wednesday: Read results *Assignment Complete lab report 12 and turn in for grading Week 13 *Identification of Unknown *Objectives include: **Use techniques from semester to determine an unknown microbe **Correctly document and identify unknown *Assignment Correctly identify unknown using techniques and procedures learned during semester Week 14 *Review prior to Final Lab Exam *Objectives include: **Retrain any labs that were missed **Prepare for final comprehensive lab exam

Week 15

*Final Comprehensive Lab Exam (10% of total grade in course)

Addendum B Laboratory Grading

Your lab grade will constitute 35% of your overall grade in the course. This 35% will be broken down as follows:

(1) Laboratory Reports/ID of Unknown (25% of total grade): You will have 12 laboratory reports to complete during the semester, each worth 100 total points. You will have one week to complete and turn in each lab report. During week 13 you will show what you have learned through the identification of an unknown microbe. This exercise will count as a lab report and will also be worth 100 points

(2) Laboratory Practical Exam (10% of total grade): During the final week of lab you will have a comprehensive exam that covers all lab objectives covered. Your lab final will be standardized to 10% of the overall grade. No make-up lab exams will be administered during the semester. Keep up with your grade during the semester using the following: Week 1 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 2 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 3 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 4 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 5 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 6 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 7 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 8 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 9 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 10 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 11 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 12 Lab Report (100 points): My grade /100 = 1.93% of total grade Week 13 ID of unknown (100 points): My grade /100 = 1.93% of total grade Week 14 (Review – No points possible): Total: /1300 = 25% of total grade Week 15 (Lab Final Exam – 100 points): Total: _____/100 = 10% of total grade *Remember that the 1300 points available for lab reports/ID of unknown will be standardized to 25% of your overall grade and the lab final will be standardized to 10% of your overall grade. This total (35%) will incorporated into your lecture grade (65%) to obtain your final grade in the course (See syllabus).