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    Global Strategy for the ex situConservation and Use of BarleyGermplasm

    10th IBGS Alexandria, Egypt, April 2008

    ICARDA

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    Global Initiatives on PGR

    1983International Undertaking on Plant Genetic Resources forFood and Agriculture, adhered to by more than 100 countries

    1996Global Plan of Action for the Conservation and SustainableUtilization of Plant Genetic Resources for Food and Agriculture

    adopted by representatives of more than 150 countries

    2003 entry into force of the International Treaty on Plant GeneticResources for Food and Agriculture, ratified by more than 100countries

    2004 - Global Crop Diversity Trust (GCDT), an endowment fund,established. Recognized as an essential element of the FundingStrategy of the International Treaty in relation to the ex situconservation and availability of PGRFA

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    GCDT Conservation Strategies

    1. To define the most efficient and effective approach for conservation atthe global crop level and at the regional level

    2. To guide the Trust in setting funding priorities

    Process:

    Involves collection holders and other stakeholders through networksand other associations

    Is facilitated by experts in consultation with stakeholders

    Gathers existing information on collections and is supplemented withsurveys and consultations

    Is backed-up from the Trust Secretariat, the CGIAR centres and FAO

    Crop and regional strategies 2 complementary approaches

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    Global Barley Conservation Strategy

    Identify the most important collections of barley (coveringthe existing genetic diversity)

    Determine collectively a global model for collaboration andsharing of responsibilities

    Assess priorities for upgrading and capacity building, ifneeded

    Identifies collections to receive conservation support overthe long term

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    Reference or key collections

    Collections on which the world depends:

    Substantial size and diversity

    Generally international or regional in coverage

    Secure - managed to international standards -

    and in general adequately funded

    Readily available on request under terms ofInternational Treaty on PGRFA

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    Other collections to be identified:

    Collections that are important (size,

    diversity, uniqueness etc.) and justify

    support from the Trust to assist withconservation and distribution

    Collections that, while of less significance in

    the context of the total global genepool,nevertheless contain important accessionsthat could usefully be duplicated in areference collection

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    Preparation of the Barley Strategy1. Lists of collections and other data assembled from sources such

    as:

    Global Inventory of Barley Genetic Resources FAO-WIEWS ECP/GR - EURISCO National/Institute Databases on internet

    2. Regional strategies completed by end 2006. Reports available for

    WANA, Asia/Pacific (SSEE Asia), E. Africa and Latin America

    3. In May 2067, more than 60 collection holders were invited tocontribute to an on-line survey. In total 25 responses received

    4. Workshop in Tunis, 4-6 September 2007, entitled: GlobalCollaborative Ex situConservation Strategy for Barley

    5. Follow-up with workshop participants and others to gatheradditional information

    6. Development of the final strategy document

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    Other elements to be included in thestrategy

    Identification of other potential partners to be involved, e.g. in storage,

    characterization, evaluation, data management, training etc.

    Identification of major gaps in the global genepool coverage and strategies

    to fill them

    A scheme for safety duplication

    Mechanisms for promoting links to users

    Effective, cross-searchable data and information management systems

    Training needs

    Any major constraints to conservation and distribution

    Coordination mechanisms (e.g. networking) and leadership

    The next steps foreseen in further developing the strategy and its

    implementation

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    Tunis workshop participation

    Countries:

    Michael Mackay (AUS), Edson Iorczewski (BRA), Bryan Harvey (CAN),Jaroslav punar (CZE), Adugna Woldesemayat (ETH), Helmut Knpffer(DEU), Javad Mozafari (IRN), Kazuhiro Sato (JPN), Hassan Ouabou(MAR), Luz Gmez (PER), Olga Kovaleva (RUS), Roland von Bothmer

    (SWE), Abdelhakim Issaoui (TUN), Mouldi Elfelah (TUN), MichaelAmbrose (GBR), Harold Bockelman (USA)

    Organizations:

    Global Crop Diversity Trust Brigitte Lalibert, Britta SkagerfltICARDA - Jan Konopka, Stefania Grando, Mohammed El Mourid, JanValkoun (consultant)

    CIMMYT Thomas Payne

    Unable to attend: Vince Logan (GRDC - AUS), Richard St-Pierre (CAN),Zhang Jing (CHN), Viktor Shevtsov (RUS), George Ayad (Bioversity)

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    The barley genetic resources

    1. Modern cultivars in current use

    2. Obsolete cultivars, often the elitecultivars of the past

    3. Landraces

    4. Wild relatives in the genus Hordeum

    5. Genetic and cytogenetic stocks6. Breeding lines

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    WILD HORDEUM SPECIES

    Tunis 2007 b courtes of R. von Bothmer

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    CAN004; 39,852

    USA005; 29,838

    BRA003; 29,227

    SYR002 - ICARDA; 26,117

    GBR011; 23,603

    DEU146; 22,106

    CHN001; 18,818

    KOR003 ; 18,764RUS001; 17,850

    ETH001; 15,350

    JPN009; 14,104

    SWE002; 13,435

    AUS003; 12,600

    Other; 120,360

    Current status

    Size of collections 402,034 total

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    0%

    10%20%

    30%

    40%

    50%

    60%70%

    80%

    90%

    100%

    CAN0

    04

    USA005

    ICARDA

    GBR0

    11

    DEU1

    46

    CHN0

    01

    KOR0

    03

    RUS001

    ETH0

    01

    JPN0

    09

    S

    WE0

    02

    AUS003

    Other

    Landraces

    Wild relatives

    Barley collections: content

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    Wild relatives;

    43588; 11%

    Landraces; 128870;

    32%

    Breeding material;

    49059; 12%

    Genetic stocks;

    25300; 6%

    Cultivars; 44003;

    11%

    Unknow n status;111214; 28%

    Global barley holdings: content

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    Overview of barley collections

    485,000 accessions FAO,1996

    371,000 accessions Hintum andMenting, 2003

    326,000 H. vulgaresubsp. vulgare

    37,000 H. vulgaresubsp. spontaneum

    1,400 H. bulbosum

    6,600 wild Hordeumspecies of the tertiarygenepool

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    Landrace major collections

    Country Genebank code No. of acces.

    ICARDA SYR002 15,496

    China CHN001 15,472

    Ethiopia ETH001 15,301

    Canada CAN004 14,074USA USA005 13,106

    Germany DEU146 12,482

    Japan JPN009 10,588

    Russia RUS001 5,132

    Korea, Rep. of KOR003 4,487

    United Kingdom GBR011 3,722

    Morocco MAR003 3,522

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    Genetic stocks major collections

    Country Genebank code No. of acces.

    Sweden SWE002 10,024

    Canada CAN004 6,011

    USA USA005 3,203

    Japan JPN009 2,890

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    Cultivar major collections

    Country Genebank code No. of acces.

    Russia RUS001 9,652

    Germany DEU146 5,172

    Canada CAN004 5,064

    USA USA005 4,394

    United Kingdom GBR011 2,739

    Poland POL003 2,404

    ICARDA SYR002 2,277

    Czech Rep. CZE047 2,161

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    Regeneration needsFirst priority to be given to wild species and geneticstocks wild species to be differentiated between secondary

    and tertiary gene pool

    Major collections, such as PGRC Canada, USDA,ICARDA and IPK Germany are in good shape andgermplasm availability is high for all the categories

    Urgent regeneration needs:

    Eritrea Ethiopia Mongolia Iran

    Peru (for long-term storage)

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    Safety duplication

    Prioritization:

    Priority 1 wild relatives landraces

    genetic stocks

    Priority 2

    cultivars

    Priority 3 breeding material

    Suggestions:

    Formal agreement in long-termstorageInclude information on safety

    duplication in the Global BarleyRegisterICARDA facilities could beused for hosting safetyduplicatesSecond level of safety

    duplication is highly desirable.Svalbard Global Seed Vaultfacilities would be mostappropriate for the secondlevel safety net

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    Number of samples distributed

    Institute code Nationally Internationally Total

    USA005 5000 3000 8000

    ICARDA 2800 2600 5400

    DEU146 1700 2300 4000

    AUS003 3300 200 3500

    RUS001 3000 400 3400

    JPN001 1300 400 1700

    CZE047 1200 300 1500

    SWE002 300 500 800

    Strengthening links to the users and increasing effectiveness of the

    collections is a priority in the global strategy for barley

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    Information and data management systems

    Individual genebanks

    Passport information is computerized in most of the collections;availability of characterization/evaluation data electronically is lower.Many accessible via internet.

    Global, regional and specialized systems

    have been developed to link different sources of locally curateddata:

    CGIAR SINGER Global Inventory of Barley Genetic Resources (GIBGR

    International Barley Information System (IBIS) EURISCO (European PGR catalogue) European Barley Database (EBDB)

    Database on Barley Genes and Barley Genetic Stocks (BGS)

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    Documentation held electronically

    Institute code Passport data Char./eval. data Web acces.

    CAN004 100% 100% Yes

    USA005 100% 100% Yes

    BRA003 100% Yes

    ICARDA 100% 100% Yes

    GBR011 100% 100% Yes

    DEU146 100% 100% Yes

    CHN001 100% 100% Yes

    KOR003 100% 80% Yes

    RUS001 100% 10% Yes

    ETH001 100% 50% Yes

    JPN009 60% 50% Yes

    SWE002 100% 20% Yes

    AUS003 100% 80% Yes

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    Information management recommendations

    Researchers and breeders will require, first of all, evaluation data

    Need to strengthen capacity of National Programs in documentationDevelopment of crop portal is suggestedEvaluate GBIF approach or other technological options foraccessing data in collectionsRevision of multi-crop passport descriptors (MCPD) should beconsidered

    Geo-referencing encouraged, important mainly for wild material andlandracesSet-specific variables/descriptors may be required, e.g. for geneticstocksCGIAR GPG2 project on Crop Registers has started. All collectionsshould become data providers for Barley Register.

    Step 1 ICARDA will develop the first versionStep 2 Advisory Group should guide the development of systemThe group: Mike Ambrose, Roland von Bothmer, Stefania Grando,Bryan Harvey, Helmut Knuepffer, Jan Konopka, Hassan Ouabou,Thomas Payne

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    Gaps and threats

    H. vulgaresubsp. spontaneum and other wild relativesare endangered, because of habitat lost by overgrazing,changes in land use and other negative human-inducedactivities.

    Landraces are gradually replaced with improvedgermplasm, but they are still grown in in low-inputfarming systems in marginal and stress-affected areas.

    To assess accurately the gaps in the ex situcollections,it first necessary to complete geo-referencing of existing

    collections as much as possible and map the collectionsites on the distribution area of natural populations ofwild relatives and landrace-growing regions

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    Networks relevant to barley GRNetworks

    Regional networks, Barley Core Collection, Generation Challenge

    Program (GCP), information networks

    Global Barley Strategy for collaboration to be strengthened:

    evaluation networks using common set of germplasm (e.g. based onBCC)

    molecular characterization/genomics diversity analysis / mapping

    climate modeling

    Suggestion/recommendation 1: GCDT could play a valuable role at theinstitutional or ministerial level to help individuals participation in meeting

    and expert panels to put a Trust mark on the individuals and networksSuggestion/recommendation 2: That the Barley Genetic ResourcesCommittee of the International Barley Genetic Symposium be re-established

    To facilitate regular meetings and to maintain momentum in completing theoverview of global resources, coordination is crucial. This might be a task ofGCDT or the Governing body of ITPGRFA.

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    Barley Core Collection

    Subset Accessions Present situation

    Ethiopia / Eritrea - not solved cooperation with Ethiopia necessary

    West Asia, NorthAfrica

    285 available from ICARDA

    South and East Asia 380 available from RIB

    Europe 298 available from IPK

    Americas 152 available from USDA

    Oceania 11 available from Tamworth Australian barleys only

    H. spontaneum 70+144 two candidate sets original ssd material & newly

    selected set of populations, available from Alnarp& ICARDA

    Other wild species 48 available from Alnarp, multiplication often difficult,partly IPK

    Genetic stocks &reference material

    - coordinators selected BCC candidates, availablefrom USDA

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    Policy issues

    Barley is included within the multilateral systemfor access and benefit-sharing under theITPGRFA, listed in the annex 1 of the Treaty.

    Countries that are parties to the Treaty have anobligation to help facilitate their collections ofbarley genetic resources available under theterms specified in the Treaty.

    Most countries represented in the survey areparties to the Treaty and 9 countries out of the39 have yet to ratify.

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    Capacity building

    The following priority activities were proposed: Training of staff in general PGR conservation Regional workshop for database managers Genebanks visits and on site training

    Training of trainers in documentation Strategies for conservation of wild material andgenetic stocks

    Training in molecular genetics and taxonomy Sequence between activities and timing issuesMost training and capacity building activities are non-crop-specific and possibly also useful for other crop andregional strategies

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    Strategic approach to conserving thebarley gene pool

    Key collections may be a basis of a global network providing diversityto international community of users

    Criteria:

    structure size, scope, uniqueness

    quality - documentation, facilities, resources access accessibility, availability

    Category:

    A high on all 3 groups of criteria - secured and well resourced Bhigh on 2 out of 3 groups - significant but may need capacity

    building

    C more information is needed

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    Country GB code Total no. ofaccessions

    Category

    A/B/C

    United States USA005 29838 A

    Germany DEU146 22106 A

    Canada CAN004 39738 AICARDA SYR002 26117 A

    Sweden SWE002 13435 A

    Japan JPN009 14106 A

    United Kingdom GBR011 9903 A

    Australia AUS003 12600 A

    Japan JPN003 8806 A

    CIMMYT MEX002 14528 A

    Netherlands NLD037 3507 A

    France FRA040 5517 A

    Czech Republic CZE047 2705 A

    Switzerland CHE001 795 A

    Korea, Rep. of KOR003 18296 A

    Spain ESP004 2421 A

    Czech Republic CZE122 1865 A

    Poland POL003 5942 A

    Hungary HUN003 4208 A

    Austria AUT001 747 A

    Slovakia SVK001 2047 A

    Key collections category A

    Total holdings A:

    ca. 240,000 acces.21 genebanks

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    Country GB code Total no. ofaccessions

    Category

    A/B/C

    Russia RUS001 17850 B

    Brazil BRA003 29227 B

    Iran IRN029 7600 B

    Ethiopia ETH001 15360 B

    Brazil BRA015 3360 B

    Turkey TUR001 1208 B

    Morocco MAR003 3743 B

    Peru 2759 B

    Category BTotal holdings B: ca. 81,000 accessions held at 8 genebanks

    Category C:Total holdings 71,000 acces. at 20 genebanksAdditional collections: 8 countries

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    Next stepsThe Tunis meeting suggested the following steps in theimplementation of the Global Strategy for Barley:

    Final version of the Strategy to be circulated to the:

    Barley Newsletter

    Barley Genetics Newsletter

    Grain GenesOpportunities to present the Strategy:

    International Barley Genetics Symposium, April 2008,Alexandria, Egypt

    ECPGR Cereal Network meeting, April 2008, Izmir, Turkey

    Barley genetics meetings (regional and national)

    Advisory Group and implementation of the Strategy

    The Global Barley Conservation Strategy Advisory Group isconstituted of the participants of the Tunis meeting

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    Advisory Group (AG) and theimplementation of the Strategy

    AG would be responsible for reviewing periodically the Strategy assessingits implementation, identifying threatened collections and orphan collections

    AG could present updated reports to the International Barley GeneticsSymposium

    A letter should be send to the IBGS International Committee from the Trust(Cary Fowler) requesting to: Re-establish the barley germplasm committee Consider questions raised in the strategy Play a facilitating role

    It was proposed that the Trust endorses this group to facilitate institutionalcommitment in its participation

    Funding be sought to hold AG meetings and the Trust assistance wasrequested

    It was proposed that ICARDA act as a clearing mechanism and the contactorganization. It was therefore proposed that the Trust (Cary Fowler) sends aletter to ICARDAs DG.

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    Acknowledgments

    Valuable contribution to the Global Barley Strategydevelopment of many involved is gratefully acknowledged.

    Thanks are particularly due to:

    Genebank managers or barley collection curators whoresponded to the Questionnaire

    Participants of the Tunis meeting

    Global Crop Diversity Trust

    ICARDA (Jan Konopka, Stefania Grando and others)

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    Thank you