baculogenes: designing an improved gene therapy … · project ‐ gene therapy: an integrated...

GENE THERAPY FOR PROSTATE CANCER ‐ 3 GIANT CONSORTIUM GENE THERAPY The role of YCR‐CRU within the GIANT consor3um is to carry out in vitro cell model tes3ng of novel gene therapy vectors and molecules:‐ LNCaP metasta3c prostate cells grown in 3D and infected with an adenovirus (blue = cell DNA, green = adenovirus). Electron microscopy showing holes observed in outer cells of an LNCaP spheroid 3 days aJer infec3on with a prostate targeted adenovirus. Damaged cells Healthy cells The aim of the EU‐funded GIANT project ‐ G ene Therapy: An I ntegrated A pproach for N eoplas3c T reatment ‐ is to develop and test novel prostate‐targeted gene therapy vectors. BPH‐1 prostate cells (turquoise = cell nuclei) bound to transferrin pep3de (red), a poten3al molecule for targe3ng gene therapy vectors. Baculovirus BACULOGENES: DESIGNING AN IMPROVED GENE THERAPY VECTOR The insect baculovirus AcMNPV is another promising gene therapy vector that is being developed by the EU funded Baculogenes project. Advantages of AcMNPV include:‐ • Large insert capacity for foreign DNA. • No pre‐exis3ng immunity (helping it evade our immune system). • Eﬃcient nuclear gene delivery to dividing and non‐dividing cells. • It can easily be produced inexpensively in large quan33es. 2 1 Our aims within the Baculogenes project are: 1. to determine what signals govern packaging of viral DNA; 2. to engineer the virus to be targeted towards prostate cancer cells and to only express therapeu3c genes within those cells.

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Page 1: BACULOGENES: DESIGNING AN IMPROVED GENE THERAPY … · project ‐ Gene Therapy: An Integrated Approach for Neoplasc Treatment ‐ is to develop and test novel prostate‐targeted

GENETHERAPYFORPROSTATECANCER‐3

GIANTCONSORTIUMGENETHERAPY

TheroleofYCR‐CRUwithintheGIANTconsor3umistocarryoutinvitrocellmodeltes3ngofnovelgenetherapyvectorsandmolecules:‐

LNCaPmetasta3cprostatecellsgrownin3Dandinfectedwithanadenovirus(blue=cellDNA,

green=adenovirus).

Electron microscopy showing holes observed inouter cells of an LNCaP spheroid 3 days aJerinfec3onwithaprostatetargetedadenovirus.

Damagedcells

Healthycells

The aim of the EU‐fundedGIANT project ‐ Gene Therapy: AnIntegrated Approach for Neoplas3c Treatment ‐ is to developandtestnovelprostate‐targetedgenetherapyvectors.

BPH‐1prostatecells(turquoise=cellnuclei)boundtotransferrinpep3de(red),apoten3almoleculefortarge3nggenetherapyvectors.

Baculovirus

BACULOGENES:DESIGNINGANIMPROVEDGENETHERAPYVECTOR

The insect baculovirus AcMNPV is another promising gene therapyvectorthatisbeingdevelopedbytheEUfundedBaculogenesproject.

AdvantagesofAcMNPVinclude:‐• LargeinsertcapacityforforeignDNA.• Nopre‐exis3ngimmunity(helpingitevadeourimmunesystem).• Efficientnucleargenedeliverytodividingandnon‐dividingcells.• Itcaneasilybeproducedinexpensivelyinlargequan33es.

Our aims within the Baculogenesproject are: 1. to determine whatsignals govern packaging of viral DNA;2. toengineer thevirus tobetargetedtowards prostate cancer cells and toonly express therapeu3c genes withinthosecells.

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