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1. Skin Preparation PDI ® Chlorascrub™ Swabstick • After location of the vein, vigorously scrub the venipuncture site with PDI ® Chlorascrub™ Swabstick for 30 seconds. 1 • Allow the site to air dry before venipuncture. • Do not re-palpate the vein. 2. Bottle Preparation • Inspect each blood culture bottle before use to ensure integrity of bottle and sensor on bottom of bottle is intact. The sensor is normally a uniform grayish-green color and a yellow color would indicate contamination of the broth. Discard any bottle found to be damaged or with a sensor that is yellow. • Remove protective flip top overcap. • NOTE: The septum is not sterile and must be disinfected. • Cleanse the septum with 70% alcohol. • The bottle has been pre-marked with 5ml increments. Mark the desired fill volume level on the bottle for 10ml. 3. Venipuncture and Bottle Inoculation Direct Draw with Blood Collection Set or WorkSafe™ Blood Culture Collection Kit • Collect all necessary materials for a direct draw: from diagnosis, the seeds of better health BacT/ALERT ® Blood Culture Collection Procedure Availability WorkSafe Blood Culture Collection Kit 279102-279105 * Saf-T Holder ® Blood Culture Device (Adaptor Cap and Insert) 100/case 96004 ** * see bioMérieux catalogue ** see Smiths Medical catalogue bioMérieux, Inc. 100 Rodolphe Street Durham, NC 27712 U.S.A. Tel: (800) 682-2666 Fax: (800) 968-9494 bioMérieux Canada, Inc. 7815 Henri-Bourassa Boulevard Ouest St. Laurent (Québec) Canada H4S 1P7 Tel: (514) 336-7321 Fax: (514) 807-0015 1-800-361-7321 ext. 221 www.biomérieux-usa.com © 2008 BIOMÉRIEUX, INC. BACT/ALERT ® AND BACT/ALERT ® 3D ARE REGISTERED TRADEMARKS OF BIOMÉRIEUX. SAF-T HOLDER ® IS A REGISTERED TRADEMARK OF THE SMITHS MEDICAL FAMILY OF COMPANIES. CHLORASCRUB™ IS A TRADEMARK OF PDI. PRINTED IN USA. BTA-125-08. Tips ’n’ Hints • Recommended blood to broth ratio is 1:5 to 1:10. As the volume of blood drawn is increased, the yield of positive cultures increases. Optimally, 20ml of blood should be drawn from adults (10ml per bottle). • When labeling the bottles, do not cover the peel-off section of the barcode labels or the lot numbers. • Orient additional patient label vertically for scanning efficiency. • For best volume control, mark fill level on side of bottle prior to collection. • Do not overfill the bottles, as this may cause false positive readings. • When using the Saf-T Holder ® Blood Culture Device (Adaptor Cap and Insert), be sure luer is connected firmly and the needle is straight when entering and leaving the septum. Twisting or turning the bottle may increase the chance that the sheath may not retract and reseal. • To avoid contamination of the blood culture sample, inoculate blood culture bottles first. Then fill additional blood collection tubes. Septicemia occurs in approximately 200,000 patients each year, and between 30 and 50% of those patients die. Because the consequences of septicemia are so serious, rapid and accurate diagnosis of this disorder is critical. The Blood Culture is the best diagnostic tool for detecting septicemia, and it’s absolutely imperative that the blood specimen be collected properly to provide clinically valuable results. BacT/ALERT ® 3D instruments are automated systems that incubate, shake and monitor culture bottles for signs of microbial growth. Samples from patients suspected for septicemia are inoculated into BacT/ALERT culture bottles for monitoring. The bottles are color coded for easy identification of media type and are made of plastic for added safety. Perform venipuncture. When the needle is in the vein, secure it with tape or hold it in place. 2. Place Adapter cap on the aerobic (blue or green label) BacT/ALERT culture bottle septum and press down to penetrate and obtain blood flow. Hold the Adapter Cap down on the bottle. 3. Using the fill indicator lines on the label, obtain 8-10ml of blood. Move the Adapter Cap from the aerobic bottle to the anaerobic (purple or orange label) bottle (if required) and continue the collection. 4. If additional blood is required for other tests, place the Adapter Insert into the Adapter Cap and snap into place. This makes the cap compatible with vacuum collection tubes. 5. When final tube is filled, remove tourniquet. Terminate the venipuncture, activate the safety shield, grab the wing with one hand and slide the wings back until it locks in place. 6. Dispose of wing and adapter cap in a sharps container. 7. SPECIMEN BAG FROZEN REFRIGERATE ROOM TEMPERATURE BIOHAZARD Label specimens and place in plastic biohazard zip lock bag. Bottles can now be safely transported via pneumatic tube system. 8. OR Gather Tourniquet, BacT/ALERT ® bottles, Saf-T Holder ® Adapter, wing, Chlorascrub™ Swabstick, gauze, alcohol prep pads. 1 per bioMérieux/Cumitech guidelines as the manufacturer cannot make this prep time recommendation 1a. Open the WorkSafe Blood Culture Collection Kit which contains all the necessary materials in a single, convenient package. 1b.

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Page 1: BacT/ALERT Blood Culture - bioMérieux Draw with Blood Collection Set or WorkSafe Blood Culture Collection Kit • Collect all necessary materials for a direct draw: from diagnosis,

1. Skin Preparation PDI® Chlorascrub™ Swabstick

• Afterlocationofthevein,vigorouslyscrubthevenipuncture sitewithPDI®Chlorascrub™Swabstickfor30seconds.1

• Allowthesitetoairdrybeforevenipuncture.

•Do not re-palpate the vein.

2.BottlePreparation • Inspecteachbloodculturebottlebeforeusetoensureintegrity

ofbottleandsensoronbottomofbottleisintact.Thesensor isnormallyauniformgrayish-greencolorandayellowcolor wouldindicatecontaminationofthebroth.Discardanybottle foundtobedamagedorwithasensorthatisyellow.

• Removeprotectivefliptopovercap.

•NOTE:Theseptumisnotsterileandmustbedisinfected.

• Cleansetheseptumwith70%alcohol.

•Thebottlehasbeenpre-markedwith5mlincrements. Markthedesiredfillvolumelevelonthebottlefor10ml.

3.VenipunctureandBottleInoculation Direct Draw with Blood Collection Set or WorkSafe™ Blood Culture Collection Kit

• Collectallnecessarymaterialsforadirectdraw:

from diagnosis,the seeds of better health

BacT/ALERT® Blood Culture Collection Procedure

AvailabilityWorkSafeBloodCultureCollectionKit 279102-279105*

Saf-THolder®BloodCultureDevice(AdaptorCapandInsert) 100/case 96004**

*seebioMérieuxcatalogue **seeSmithsMedicalcatalogue

bioMérieux, Inc.100RodolpheStreetDurham,NC27712U.S.A.Tel: (800)682-2666Fax: (800)968-9494

bioMérieux Canada, Inc.7815Henri-BourassaBoulevardOuestSt.Laurent(Québec)Canada H4S 1P7Tel:(514)336-7321Fax:(514)807-00151-800-361-7321ext.221 www.biomérieux-usa.com

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Tips ’n’ Hints•Recommendedbloodtobrothratiois1:5

to1:10.Asthevolumeofblooddrawnisincreased,theyieldofpositiveculturesincreases.Optimally,20mlofbloodshouldbedrawnfromadults(10mlperbottle).

•Whenlabelingthebottles,donotcoverthe peel-offsectionofthebarcodelabelsorthe lotnumbers.

•Orientadditionalpatientlabelverticallyfor scanningefficiency.

•Forbestvolumecontrol,markfilllevelonsideofbottlepriortocollection.

•Donotoverfillthebottles,asthismaycause falsepositivereadings.

•WhenusingtheSaf-THolder®BloodCultureDevice(AdaptorCapandInsert),besureluerisconnectedfirmlyandtheneedleisstraightwhenenteringandleavingtheseptum.Twistingorturningthebottlemayincreasethechancethatthesheathmaynotretractandreseal.

•Toavoidcontaminationofthebloodculturesample,inoculatebloodculturebottlesfirst. Thenfilladditionalbloodcollectiontubes.

Septicemiaoccursinapproximately200,000patientseachyear,andbetween30and50%ofthosepatientsdie.Becausethe consequencesofsepticemiaaresoserious,rapidandaccuratediagnosisofthisdisorderiscritical. TheBloodCultureisthebestdiagnostictoolfordetectingsepticemia,andit’sabsolutelyimperativethatthebloodspecimenbecollectedproperlytoprovideclinicallyvaluableresults. BacT/ALERT®3Dinstrumentsareautomatedsystemsthatincubate,shakeandmonitorculturebottlesforsignsofmicrobialgrowth. SamplesfrompatientssuspectedforsepticemiaareinoculatedintoBacT/ALERTculturebottlesformonitoring.Thebottlesarecolor codedforeasyidentificationofmediatypeandaremadeofplasticforaddedsafety.

SPECIMEN BAG

FROZEN REFRIGERATE

ROOM TEMPERATURE

BIOHAZARD

Performvenipuncture.Whentheneedleisinthevein,secureitwithtapeorholditinplace.

2.

SPECIMEN BAG

FROZEN REFRIGERATE

ROOM TEMPERATURE

BIOHAZARD

PlaceAdaptercapontheaerobic(blueorgreenlabel)BacT/ALERTculturebottleseptumandpressdowntopenetrateandobtainbloodflow.HoldtheAdapterCapdownon thebottle.

3.

SPECIMEN BAG

FROZEN REFRIGERATE

ROOM TEMPERATURE

BIOHAZARD

Usingthefillindicatorlinesonthelabel,obtain8-10mlofblood.MovetheAdapterCapfromtheaerobicbottletotheanaerobic(purpleororangelabel)bottle(ifrequired)andcontinuethecollection.

4.

SPECIMEN BAG

FROZEN REFRIGERATE

ROOM TEMPERATURE

BIOHAZARD

Ifadditionalbloodisrequiredforothertests,placetheAdapterInsertintotheAdapterCapandsnapintoplace.Thismakesthecapcompatiblewithvacuumcollectiontubes.

5. SPECIMEN BAG

FROZEN REFRIGERATE

ROOM TEMPERATURE

BIOHAZARD

Whenfinaltubeisfilled,removetourniquet.Terminatethevenipuncture,activatethesafetyshield,grabthewingwithonehandandslidethewingsbackuntilitlocksinplace.

6. SPECIMEN BAG

FROZEN REFRIGERATE

ROOM TEMPERATURE

BIOHAZARD

Disposeofwing andadaptercapin asharpscontainer.

7. SPECIMEN BAG

FROZEN REFRIGERATE

ROOM TEMPERATURE

BIOHAZARD

Labelspecimensandplaceinplasticbiohazardziplockbag.Bottlescannowbesafelytransportedviapneumatictubesystem.

8.

OR

GatherTourniquet,BacT/ALERT®bottles, Saf-THolder®Adapter,wing,Chlorascrub™Swabstick,gauze,alcoholpreppads.

1perbioMérieux/Cumitechguidelinesasthemanufacturercannotmakethispreptimerecommendation

1a.OpentheWorkSafeBloodCultureCollectionKitwhichcontainsallthenecessarymaterialsinasingle,convenientpackage.

1b.

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