bacillus
TRANSCRIPT
BacillusBacillus
Aerobic bacilli-B.anthracisAerobic bacilli-B.anthracis
Anerobic bacilli-Clostredium tetaniAnerobic bacilli-Clostredium tetani
Bacillu anthracis Bacillu anthracis
Gram +veGram +ve Aerobic spore bearing bacilliAerobic spore bearing bacilli Thermophilic and psuchrophilicThermophilic and psuchrophilic B.cereus-food borne gastroenteritisB.cereus-food borne gastroenteritis Produces endosporesProduces endospores
First bacterium observed under microscopeFirst bacterium observed under microscope First communicable d/s transmitted by inoculation of First communicable d/s transmitted by inoculation of
infected bloodinfected blood First to be isolated In pure culture and used for the First to be isolated In pure culture and used for the
preperation of attenuated vaccinepreperation of attenuated vaccine Used in biological warfereUsed in biological warfere One of the largest pathogenic bacteriaOne of the largest pathogenic bacteria Capsulated PP capsuleCapsulated PP capsule M’Fadyean’s reaction-Polychrome methylene blue M’Fadyean’s reaction-Polychrome methylene blue
for few seconds-purplish material around the bacillusfor few seconds-purplish material around the bacillus Bamboo stick appearencesBamboo stick appearences NonmotileNonmotile Spores – central /terminalSpores – central /terminal
Culture Culture
Agar plates –frost glass appearanceAgar plates –frost glass appearance Low power –medusa plate appearanceLow power –medusa plate appearance Gelatin stab culture-inverted fir treeGelatin stab culture-inverted fir tree Blood agar-nonhemolyticBlood agar-nonhemolytic Selective medium-PLET medium-Selective medium-PLET medium-
Virulence factorsVirulence factors
Capsular PPCapsular PP Anthra toxinAnthra toxin Pathogenicity testing by inoculation into Pathogenicity testing by inoculation into
guinea pigsguinea pigs
AnthraxAnthrax
ZoonosisZoonosis Human d/s from animal-direct /indirectHuman d/s from animal-direct /indirect d/s may bed/s may be
1.1. CutaneousCutaneous
2.2. PulmonaryPulmonary
3.3. IntestinalIntestinal
All leading to fatal septicemia/meningitisAll leading to fatal septicemia/meningitis
Cutaneous Cutaneous
Entry thru skinEntry thru skin Satellite lasions arranged around a central Satellite lasions arranged around a central
necrotic lesion which is coverd by a black necrotic lesion which is coverd by a black eschar- the name anthrax meaning coal comes eschar- the name anthrax meaning coal comes from the black colour of escharfrom the black colour of eschar
These lesions are called Malignant pustulesThese lesions are called Malignant pustules Hide porter’s d/s-common in dock workers-Hide porter’s d/s-common in dock workers-
carrying loads and skins on their bare back.carrying loads and skins on their bare back. Generally resolves spaontaneouslyGenerally resolves spaontaneously
Pulmonary Pulmonary
Wool sorte’s d/sWool sorte’s d/s Inhalation of infected wool particlesInhalation of infected wool particles Haemorrhagic pneumonia with high fatality Haemorrhagic pneumonia with high fatality
ratesrates Haemorrhagic meningitis is a main Haemorrhagic meningitis is a main
complicationcomplication
Intestinal Intestinal
RareRare Violent enteritis Violent enteritis Bloody diarrhoeaBloody diarrhoea Primitive communities who eat carcasses of Primitive communities who eat carcasses of
animalsanimals
Lab d/gLab d/g
Microscopy, culture,M’fadyeans reaction Microscopy, culture,M’fadyeans reaction Serology-CFT,ELISASerology-CFT,ELISA Ascol’s thermo precipitation test-Ascol’s thermo precipitation test-
demonstration of anthrax antigen in tissue demonstration of anthrax antigen in tissue extractsextracts
Nucleic acid detectionNucleic acid detection
Treatment Treatment
DOC-PenicillinDOC-Penicillin StreptomycinStreptomycin DoxycyclineDoxycycline
ProphylaxisProphylaxis
Improvement of factory hygieneImprovement of factory hygiene Proper sterilizationProper sterilization Proper disposal of animal carcassesProper disposal of animal carcasses Vaccination of animalsVaccination of animals