azacitidine as maintenance therapy following hematopoietic...
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Azacitidine as Maintenance Therapy
Following Hematopoietic Stem Cell
Transplantation for AML/MDS
A Single Center Experience
André Dias Américo
Médico Residente Transplante de Medula Óssea
Hospital Israelita Albert Einstein
Introduction
Azacitidine
Blast
Immunogenic
Antigens
de Lima, M et al. Maintenance Therapy With Low-Dose Azacitidine After Allogeneic HSCT for Recurrent AML or
MDS, Cancer 2010;116:5420-31
CD4+ Treg T CD8+
CD25+
CD127lo
FoxP3+
CD137+
CCR7+
Goodyear, OC et al. Azacitidine augments expansion of regulatory T cells after allogeneic stem cell transplantation
in patients with AML , Blood 2012;119(14):3361-3369
MAGE WT1 RAGE
Introduction
Azacitidine
• Safely administered after HSCT ª º
• Phase I/II Clinical Trials OS-2yr PFS-2 yr approximately
50% after RIC conditioningª º
• Better OS and PFS related with specific CD8 response
and earlier administration (< 90 days) º
• Retrospective study with neutral results after MAC
conditioningªª
ªde Lima, M et al. Maintenance Therapy With Low-Dose Azacitidine After Allogeneic HSCT for Recurrent AML or MDS,
Cancer 2010;116:5420-31
ºCraddock, C et al. Tolerability and Clinical Activity of Post-Transplantation Azacitidine in Patients Allografted for Acute
Myeloide Leukemia Treated on RICAZA Trial, Biol Blood Marrow Transplant 2016; 22: 378-393
ªªMaples, KT et al. Maintenance Azacitidine after myeloablative hematopoietic cell transplantation for myeloid
malignancies, Leuk & Lymph 2018; DOI: 10.1080/10428194.2018.1443334
Study Design:
Retrospective Cohort
Inclusion:
Age > 18 yo
AML or MDS diagnosis
HSCT followed by Azacitidine maintenance (in complete
morphological remission)
Outcomes:
Overall Survival
Relapse Free Survival
Statistical analysis: RR Core Team (2017). R: A language and environment for statistical computing.
R Foundation for Statistical Computing, Vienna, Austria.
URL https://www.R-project.org/
Methods
HSCT
Remission
Induction Morphological
Complete
Remission
D0 ~ D30
Aza
C1
Aza
C2
Aza
C24…
GVHD prophylaxis:
ATG (MUD)
either CsA or FK506
MiniMTX
Methods
Results 76 HSCT
for AML/MDS
2011-2018
17
Azacitidine Cohort
8 Early Deaths
42 did not received
azacitidine
9 azacitidine
as relapse treatment
+/- DLI
4
Completed/Ongoing
Treatment
13
Suspended Treatment
Death = 2
Personal = 1
Relapse = 2
Toxicity = 2
Missing = 6
Variable n = 17
Male (%) 9 (52,9%)
AML / MDS 14 / 3
Age (median) 53 [21-62] yrs
Active Disease (%) 6 (35%)
DRI Intermediate (%) 8 (47,1%)
MAC (%) 11 (64,7%)
PBSC Graft Source (%) 7 (41,2%)
Time to Aza (median) 46 [42-75] days
Aza Cycles (median) 3 [2-16]
DRI High/V. High (%) 9 (52,9%)
Table 1
8 Relapses/Death (47%)
Results - Progression Free Survival
Event
Control Aza
Median PFS ~ 3 years
6 Deaths (47%)
Results - Overall Survival
2 GVHD
2 Relapse
2 Infection
Control Aza
Survival
In this setting of high risk patients we
encountered somewhat lower relapse and death
rates than previously reported
Strenghts
• CIBMTR data center/data manager
• FACT accreditated center
Limitations
• Retrospective nature
• Small sample size
• Not the ideal setting to establish the role of
azacitidine in the post HSCT setting
Conclusions
Azacitidine as Maintenance Therapy
Following Hematopoietic Stem Cell
Transplantation for AML/MDS
A Single Center Experience
André Dias Américo, Larissa Lanes Teixeira, Luis Jorge
Santos Matos Filho, Cinthya Corrêa da Silva, Mariana Nassif
Kerbauy, Andreza Alice Ribeiro, Nelson Hamerschlak
Disease Status at the Time of Azacitidine Initiation
Morphological Remission: 17 (100%)
MDR positive by Flow Citometry: 4 (23,5%)
Chimerism > 90%: 16 (94%)
Preemptive Donor Lymphocyte Infusions: 0
Predictors of Relapse
High Risk Karyotype: 7 (42%)
Disease status prior to HSCT
2 RC: 4 (23,5%)
Active Disease: 6 (35%)
Motive n = 13
Death (%) 2 (15,3)
Personal (%) 1 (7,6)
Relapse (%) 2 (15,3)
Toxicity (%) 2 (15,3)
Missing (%) 6 (46,1)
Median Azacitidine Cycles: 3
Time to Aza (median) 46 [42-75] days
Azacitidine Treatment
VariableAza
(n=17)
AML / MDS 14 / 3
Age (mean) 51 yrs
DRI Intermediate (%) 8 (47,1%)
MAC (%) 11 (64,7%)
PBSC Graft Source (%) 7 (41,2%)
DRI High/V. High (%) 9 (52,9%)
Control
(n=51)
31/20
56 yrs
29 (56%)
22 (43,1%)
18 (35,2%)
21 (41,2%)
Second Complete
Remission (%)7 (13,2%)
Secondary AML (%)
4 (23,5%)
3 (17,6%) 5 (9,8%)
Progression Free Survival Overall Survival
Results - PFS and OS
Median PFS ~ 3 yrs Median OS Not Reached
Cumulative Incidence of Relapse
Relapse 7 (41%)
Univariate
Model
HR (IC 95%)
Multivariate
Model
HR (IC 95%)p p
Azacitidine
DRI Intermediate
0,50 (0,2-1,21) 0,1
0,44 (0,22-0,89) 0,02
0,41 (0,17-1,0) 0,009
0,39 (0,19-0,78)
Overall Survival
Progression Free Survival
0,005Azacitidine
DRI Intermediate
0,28-1,34 0,2
0,41(0,21-0,79) 0,008
0,49 (0,22-1,09)
0,36 (0,18-0,71)
events = 35
events = 38
Results - Cox Regression
All Grade < 3
Mostly Skin
1 Severe GVHD
Graft Versus Host Disease