automation of genetic design - dels microsite...
TRANSCRIPT
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Our funders:
Automation of Genetic Design
National Academy of Sciences
Industrialization of Biology Workshop
February 17, 2014
J. Christopher Anderson
Assistant Professor, Bioengineering
512E EBB, UC Berkeley
http://andersonlab.qb3.berkeley.edu
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Synthetic
Biology
Today
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Automated
Synthetic
Biology
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synbiotrails.org Coming June 11th!
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Act Ontology
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Exhaustively compute the biosynthetically reachable space Enumerate all paths to each reachable chemical Rank those pathways for plausibility Provide linkage to the DNA sequences (parts) that encode each enzyme
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True
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The L0-L2 Reachable Space
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Filtering the Reachable Space
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Filtering the Reachable Space
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Validation of an L2 target: Tylenol
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Known Prediction Holes
Expression in foreign host Chemical modifications Multiple subunits Kinetic parameters Membrane/organellar localization Spontaneous reactions Volatile products
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Big-Picture Bottlenecks
Intellectual property / freedom to operate Biosafety/biosecurity checks Regulatory analysis Target coordination Polyketides/Non-ribosomal peptides Polysaccharides Complex lipids Genetic circuits