automated preparation of chromosome and fish slides€¦ · 17/08/2016 · manual preparation 2.5...
TRANSCRIPT
![Page 1: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/1.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 1
Automated Preparation of Chromosome and FISH Slides
![Page 2: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/2.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 2
Presenter
Jim Stanchfield, Ph.D.SciGene Founder and CEOSunnyvale, CA
![Page 3: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/3.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 3
About SciGene
• Founded in 2004 to develop solutions for relieving pain points in cytogenetic workflows
• Provides innovative FISH workflow equipment and associated reagents
We supply everything for FISHexcept probes and microscopes!
![Page 4: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/4.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 4
Most Labs Lose Money Running FISH
2018 reimbursement: $51 per hybridization(CPT 88275 Interphase FISH 100-300 cells)
![Page 5: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/5.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 5
Setting up FISH Panels is a Lot of Work
• Difficult to finish daily workload• Requires multiple slides and coverslips per patient • Tedious to seal coverslips with rubber cement
![Page 6: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/6.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 6
Typical FISH MethodSix Probe Panel Uses $150 – 300 of Probe
3 6
30-60 μl probe
Slides Coverslips Rubber Cement
5 - 10 µl
![Page 7: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/7.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 7
MICROFISH® ASSAY SYSTEM
![Page 8: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/8.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 8
MicroFISH Assay System
A simple system for performing cellular FISH panels
that uses a single slide per patient and 1 µl cell sample
and 1 µl probe solution per well.
![Page 9: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/9.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 9
MicroFISH Assay Slide
Coating retains samples in wells.1 µl wells conserve samples.
Wells numbered individually.1 coverslip — No sealant.
Single Patient Slide for up to 8 Probes22x 50 mm coverslip
![Page 10: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/10.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 10
Simple Workflow
1 2 3 4MicroFISH® Hyb Oven
INCUBATELittle Dipper® for FISH
AUTOMATED WASHDrop Cells
Add Probes
MANUAL PREPCytoBrite® PLUS System
DENATURE
Place Coverslips
![Page 11: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/11.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 11
New CAP Regulation for CytogeneticsISH Slide Processing System Temperature Checks
**NEW** CAP REGULATIONCYG.33950 ISH Slide Processing System Temperature Checks Phase IIIndividual slide slots (or a representative sample thereof) of in situ hybridization (ISH) temperature controlled slide processing systems are checked for temperature accuracybefore being placed in service and at least annually thereafter.
Evidence of compliance:√ Written procedure for verification of temperature accuracy AND√ Records of equipment verification
Cytogenetics Checklist | 08.17.2016
![Page 12: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/12.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 12
CytoBrite® PLUS Slide Incubation System Monitors Temperature of Every Slide Position
• Meets new CAP Regulation CYG.33950• PCR Technology rapidly heats/cools slides • Run data collected on USB key for record keeping• Slide trays streamline handling
![Page 13: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/13.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 13
CytoBrite PLUS Slide Incubation System Slide Temperature Verification
• Each slide position equipped with temperature sensor wired to a jack
• Connect the provided thermometer to view each slide position temperature
![Page 14: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/14.jpg)
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1 2 3 4 5 6 7 8 9 10 11 12
Tem
pera
ture
(°C)
Time (min)
Allowable range
CytoBrite PLUS Slide Temperature Uniformity
![Page 15: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/15.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 15
MicroFISH Hybridization Oven
• For incubating MicroFISH® slides• Boosts probe signals • Holds up to 162 MicroFISH slides• Slide trays transferred directly from
CytoBrite System
Controls Temperature and Humidity
![Page 16: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/16.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 16
Little Dipper® ProcessorMultipurpose Cytogenetic Slide Processor
G Banding
Post-hybProcessing
Tissue Pretreatment
![Page 17: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/17.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 17
Little Dipper Processor Automates MicroFISH Coverslip Removal + Washing
Coverslips shaken offand slides washed
Coverslips retrieved from basket
Slides centrifuged dry and removed
![Page 18: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/18.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 18
MicroFISH SystemReduces Probe Cost Per Test up to 90%
3 6 1 1
Typical Method MicroFISH Method
Probe cost: $150 - 300 Probe cost: $30
![Page 19: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/19.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 19
SCORPION™ SLIDE PREPARATION ROBOT
![Page 20: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/20.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 20
Scorpion RobotAutomates Chromosome and MicroFISH Slide Prep
• Smaller than a lab incubator• Fast – Makes 12 chromosome slides in < 10 minutes• Fast – Makes 12 MicroFISH slides in < 30 minutes• Controlled humidity for optimal metaphase spreading• Produces detailed patient reports• Tracks probe inventory
![Page 21: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/21.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 21
Scorpion Robot VIDEO
Find link on SciGene home page
![Page 22: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/22.jpg)
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Scorpion Robot for MicroFISH SlidesDeck Set Up
← Probe tubes
← Pipet ps
12 patient sample tubes with fixed cells ↓
12 MicroFISHslides in two slide trays →
![Page 23: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/23.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 23
Scorpion Robot for MicroFISH SlidesMakes 12 Slides in 25 Minutes
Robotic Steps Time (min)
1. Upon start, robot dispenses 1 µl of patient cells into designated wells across 12 slides
3
2. Robot pauses to allow slides to dry. 10
3. Robot dispenses 1 μl of probe into designated wellsacross 12 slides
12
Elapsed Time: 25 min
![Page 24: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/24.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 24
Scorpion Robot for MicroFISH SlidesSaves 2.5 Hours Labor per 12 Patients
Manual Preparation
2.5 hrs
Automated Slide Prep
5 min
![Page 25: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/25.jpg)
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Scorpion Robot for MicroFISH Slides
• Details every FISH probe hybridization • Includes probe names/lot numbers / expiration dates
Generates Detailed Patient Reports
![Page 26: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/26.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 26
Scorpion Robot for Chromosome SlidesDeck Set Up
← Pipet ps
Patient sample tubes with fixed cells ↓
12 slides in two slide trays →
![Page 27: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/27.jpg)
SciGene, Sunnyvale, CA | 1.408.733.7337 | [email protected] | www.scigene.com 27
Humidity Effects on Chromosome Spreading Using a Cell Dropping Robot
• Chromosome slides prepared at humidity levels between 25-70% RH
• Slides G-banded and 20 metaphases per slide analyzed by a certified cytogeneticist
![Page 28: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/28.jpg)
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Automated Preparation of Chromosome Slides
40% RH
Humidity Effects on Spreading
Quality # Spreads %
Good 4 20%
Fair 15 75%
Poor 1 5%
25% RH
Quality # Spreads %
Good 0 0%
Fair 3 15%
Poor 17 85%
![Page 29: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/29.jpg)
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50% RH 55% RH
Quality # Spreads %
Good 14 70%
Fair 6 30%
Poor 0 0%
Quality # Spreads %
Good 10 50%
Fair 9 45%
Poor 1 5%
Automated Preparation of Chromosome SlidesHumidity Effects on Spreading
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60% RH 70% RH
Quality # Spreads %
Good 13 65%
Fair 7 35%
Poor 0 0%
Quality # Spreads %
Good 4 20%
Fair 16 80%
Poor 0 0%
Automated Preparation of Chromosome SlidesHumidity Effects on Spreading
![Page 31: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/31.jpg)
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A Complete Slide Processing Workflow
![Page 32: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/32.jpg)
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Flexible Configuration Chromosome Slides
Scorpion™ Robot
Automated G-banding
Little Dipper® Processor
Manual G-banding
![Page 33: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/33.jpg)
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Flexible Configuration MicroFISH Slides
Manual Prep Automated Prep
Automated Post-HybManual Post-Hyb
Scorpion™ Robot
Little Dipper® Processor
CytoBrite PLUS System with MicroFISH Oven
>> CORE SYSTEM <<
![Page 34: Automated Preparation of Chromosome and FISH Slides€¦ · 17/08/2016 · Manual Preparation 2.5 hrs Automated Slide Prep 5 min. SciGene, Sunnyvale, CA | 1.408.733.7337 | custserv@scigene.com](https://reader035.vdocuments.site/reader035/viewer/2022071101/5fdabcbb1b983c1de9408c9f/html5/thumbnails/34.jpg)
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Schedule a demo running your samples and probes
BOOTH 1009
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Automated Preparation of Chromosome and FISH Slides