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Atomic Absorption & Emission Spectroscopy Chapter 5 Slide 2 Sub Topics Introduction to Atomic Spectroscopy Atomic Absorption Spectroscopy AAS Atomic Emission Spectroscopy AES Interferences and Comparisons Slide 3 Atomic Absorption and Emission Spectroscopy What is it used for? Study of trace metals in the environment Examples: Contamination of water Food stuffs Petrol products Quality control Qualitative and Quantitative method for >70 elements with detection levels in ppm/ppb range Fast and convenient with high selectivity Slide 4 Three Types Done in the Gas Phase Why is this significant? No vibrational or rotational energy levels, only electronic transitions Therefore spectra made of quantised spectral lines Spectral ranges of interactions? 180 800 nm (UV-Vis) Where is electromagnetic radiation absorbed / emitted in each spectroscopy? Slide 5 Spectrum Related to Electronic Configuration(s) Slide 6 Basic Instrumentation Requirements: Atoms / Ions in gas phase AES: No Radiation Source AAS: As Shown AFS: Radiation Source at 90 to Detector Slide 7 Atomisation Sample needs to be in gas phase Atomisation is therefore crucial Several ways: AES Inductively Coupled Plasma (ICP) AAS Flames Electrothermal Atomizers Two Types of Atomisation ContinuousSample Continuously Introduced DiscreteSample introduced by syringe/autosampler Slide 8 Sub Topics Introduction to Atomic Spectroscopy Atomic Absorption Spectroscopy AAS Atomic Emission Spectroscopy AAS Interferences and Comparisons Slide 9 Process of Atomisation Nebulization Production of mist / spray Commonly used for solutions. Solids: Electric Spark or Laser SampleSprayDry-AerosolFree Atoms / Molecules / Ions Nebulization Desolvation Volatilization Slide 10 Example of Atomisation Process Flames Geometry of a Flame Different Fuels Different fuels give different temperatures Normal FlameAlkali/Alkaline Metals1700-2400C O 2 or N 2 OHeavy Metals2500-3100C Slide 11 Sample Introduction and Atomisation Pneumatic Nebulizer Continuous Achieved using Venturi Effect Jet effect to form mist / spray Slide 12 Interferences Any effect that changes signal from proper signal Blank Interferences Spectral Analyte (multiplicative) Interferences Physical (matrix) Chemical Ionisation Slide 13 Spectral Interferences Independent of Analyte concentration From elements other than Analyte E.g. Na (285.28 nm) overlaps Mg (285.21)nm Can subtract blank Na spectrum Results in Line Broadening Spectral lines have finite widths, but determined by spectrometry properties Not needed to know different types Slide 14 What if too concentrated? Slide 15 Sub Topics Introduction to Atomic Spectroscopy Atomic Absorption Spectroscopy AAS Atomic Emission Spectroscopy AES Interferences and comparisons Slide 16 Inductively Coupled Plasma Plasma Atomisers Plasma is a conducting gaseous mixture containing cations and anions. Argon typically used for AAS Once Argon ions formed, can absorb enough power from external source to reach high temperatures so further ionisation can occur (T > 10,000 K) Power Sources DC Electric Powerful Radio Frequency (RF) Generators Powerful Microwave Frequency (MW) Generators (ICP) RF / ICP provide best advantage, sensitivity and freedom from interference DC Plasma cheaper and simpler Slide 17 Inductively Coupled Plasma Three concentric quartz tubes End of torch is a RF induction coil which induces magnetic field Gases (Ar or N 2 ) flow in outer tubes to cool torch. Stabilizes plasma. Torch is seeded with electrons using spark. H accelerates to high energy to ionise gases. White hot fireball formed (8000 10000 K) Slide 18 Inductively Coupled Plasma Sample (from nebulizer, + Ar gas) is injected into it via central tube. Advantages Higher Excitation Temperature than any flame Lower detection limits Wide range of metals and non-metals Disadvantages Many Lines Initial and Running Costs Large! Other Points Long, Linear Calibration Curves (4-5 orders magnitude) Useful for both Atomic Spectroscopy and Mass Spectrometry Slide 19 Comparison ICP vs DC ICP Advantages Chemically inert environment Unlike a flame (violent, highly reactive) Temperature cross-section constant Thin optical path Reduces self-absorption Calibration linear over large orders of concentration Disadvantages Does not like organic solvents Clogging due to carbon build up DC and other Plasma Sources Advantages Less Argon Needed Can cope with organic solvents Less expensive Less sensitive Good Reproducibility Disadvantages Useful for atoms, not ions Good alignment of optics Electrodes need to be replaced Slide 20 Recap so far Elemental Analysis ICP Most common source Need Light Source Flame most common source Slide 21 Some more equations Slide 22 Absorbance Slide 23 Requirements for Source Atomic Absorption Spectroscopy AAS Narrow resonance line profile from lamp Stable and reproducible output Enough intensity for good S/N ratio Durability Easy Operation Hollow Cathode Lamp Good Choice! Slide 24 Atomization AAS FlameAir or N 2 O combined with acetylene Eletrothemal AtomizerGraphite furnace Background Corrections may be required Chemical Interference Continuum Source Corrections Pulsed Hollow Cathode Lamp Corrections Zeeman Effect Slide 25 Electrothermal Atomizers: Graphite Furnace AAS Drawbacks of AAS/AES/AFS in General Large volumes Poor efficiency nebulization Aqueous solutions required Electrothermal Atomizer: Graphite Furnace (AAS/AES only) Heated with inert purge gas All sample is atomized in confined space! Small volume in furnace with a syringe Programmed heating Slide 26 GF-AAS Procedure 1. Introduce sample 1. 5-50 microlitres or solid 2. Add modifier 3. Drying 1. 110C 4. Pyrolysis/Ashing 1. 300-1100C 5. Evaporation of matrix? 6. Atomisation and measurement time resolved 1. 2000-3000C 7. Clean out 8. Cool down Whole process takes milliseconds! Slide 27 GF-AAS Interferences Corrections are needed Matrix Modifier Dual Lamp setup T selective programing Zeeman Correction Slide 28 Zeeman Effect Correction Strong Magnetic Field splits degenerate spectral lines into several lines according to their M j values, which have different polarization characteristics Example, Magnesium Slide 29 Zeeman Background Correction Slide 30 Sub Topics Introduction to Atomic Spectroscopy Atomic Absorption Spectroscopy AAS Atomic Emission Spectroscopy AES Interferences and comparisons Slide 31 Interferences Ionization Loss of atoms as ions give completely different spectra Can be supressed by adding ionization suppressor (e.g. Cs) to sample and reference Chemical Key problem for AAS Formation of chemical species which prevent/promote dissociation, giving rise to depressed/enhanced signals Requires releasing/protective agents Slide 32 Calibration Very Important Encompass range of expected values for samples Blank Triplicate to determine detection limit Standard addition method required for high matrix samples Slide 33 Comparison What is less prone to spectral interferences? AAS AES Temperature affects signal more in? AES / AFS AAS Because ground state population is largest Slide 34 Rank the following in Limit of Detection? AES, ICP-AES, AAS, GF-AAS, AFS AES>AFS>AAS>ICPAES>GF-AAS Rank the following in dynamic working range? AES, ICP-AES, AAS, GF-AAS, AFS ICP-AES>AFS>AES>AAS>GF-AAS Slide 35 Other Comparisons AAS Ratio method removes systematic errors but sensitivity affected by difference in two large quantities ICP-AES and AFS can be multi element, less so than ASS AAS most cost effective, ICP-AES most versatile AFS good for vapour phase elements Cost of instrumentation ICP-AES>GF-AAS>AAS>AFS>AES