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JOANNE LOMAS-NEIRA Ph.D.3 Watercress Court * Coventry, Rhode Island 02816401.439.3610 * jl85686e@westpost.net

SENIOR BIOLOGICAL SCIENTIST

Dynamic PhD scientist with over 10 years experience in molecular/cell biology, immunology and disease pathology. Successfully training and mentoring undergraduate and graduate students in human biology and clinical laboratory science for 8years. Proven analytical skills. Published in American Journal of Physiology, books, and numerous scientific journals. Demonstrated expertise in efficiency optimization and cost control. Combined scientific background with business acumen developed during tenure as a private sector senior manager. Certified in ProjectManagement.

Cell & Molecular Biology * Pathology * In vivo Disease ModelingEducation/mentoring * Project & Laboratory Management * ResearchEfficiency Optimization * Data Analysis * Team Leadership & Development* Publications & Abstracts * Presentations * Protocol AnalysisCost Containment * Client & Vendor Relations * Budgeting & Forecasting

RESEARCH EXPERIENCE

RHODE ISLAND HOSPITAL/BROWN UNIVERSITY, Providence, Rhode Island * 1999 to presentDr. Alfred Ayala's laboratory (focusing on sepsis-related immune dysfunction) inthe Department of Surgical Research for a major urban hospital and world-renowned university.

Instructor in Surgery-Research (previously Post Doctoral Fellow)Published 12 first author manuscripts, review articles, co-authored additional manuscripts, book chapters and wrote numerous abstracts. Designed and oversaw experiments and delivered presentations at national and international conferences.Developed protocols and training for new technology implementation. Trained labo

ratory technicians and students in surgical, experimentation, histology, protein/gene characterization assays and data analysis techniques.* Designed and conducted experiments to examine therapeutic potential of siRNA as a short-term suppressant of gene expression in vivo, resulting in a request for a manuscript, review article and presentations at conferences in the US, Canada and the United Kingdom.* Developed mouse lung primary endothelial-cell culture monolayer model of vascular injury* Advancing the understanding of the role of murine G-protein coupled receptor,CXCR2, in acute lung injury, through original research* Developed proficiency in MS PowerPoint, Word and Excel, as well as Flowcytometric, image analysis and database management software.

COMMUNITY COLLEGE OF RHODE ISLAND, Warwick, Rhode Island * 2007-2008Department of Biological Sciences with associate and pre-professional college curriculum

Adjunct Professor* Teaching Cell Biology lecture and laboratory and Human AnatomyJOANNE LOMAS-NEIRA * Page 2 * jlomasneira@verizon.net

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BROWN UNIVERSITY, Providence, Rhode Island * 1998 to 1999Dr. Boris Rotman's university and NIH-funded laboratory research project focusing cell-based cancer detection from patient tissue samples.

Research AssistantPerformed experiments and advised on potential protocol modifications. Studied underlying concepts of Dr. Rotman's research paradigm and critiqued collected data. Isolated and cultured human cancer cells for experimentation. Conducted immunoassays. Maintained equipment, lab notebook, and materials inventory.

REPRESENTATIVE PUBLICATIONS

Thakkar, R.H., Huang, X., Lomas-Neira, J., Heffernan, D. Ayala, A. Sepsis and the Immune Response. In: Essential Immunology for Surgeons. Editor: E. Eremin. Oxford Press, UK. 2010 (in press).Venet,F., Chung, C.S., Huang,X., Lomas-Neira, J., Chen, Y., Ayala, A. Lymphocytes in the development of lung inflammation: A role for regulatory CD4+ T cells inindirect pulmonary lung injury. J. Immol. (2009)

Perl, M., Chung, C.S., Perl, U., Lomas-Neira, J., Ayala, A. Therapeutic Accessability of caspase-3 activation as a key pathomechanism in septic acute lung injury. Critical Care Med. (2009)

Perl, M., Lomas-Neira, J., Chung, C.S., Ayala, A. 2008. Epithelial cell apoptosis and neutrophil recruitment in acute lung injury-A unifying hypothesis? What we have learned from small interfering RNAs. J Mol Med 14(7) (2008) Online.

Lomas-Neira, J.L., Chung, C.S., Ayala, A. RNA Interference As A Potential Therapeutic Treatment For Acute Lung Injury. Int J Clin Exp Med 2, 154-160 (2008).

Wesche-Soldato, D.E., Lomas-Neira, J., Perl, M., Chung, C.S., Ayala, A. Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis. In: Methods in Molecular Biology Series: siRNA, shRNA and miRNA. Editors: S. Barik. Human Press Inc., Totowa, NJ, pg (2008).

Lomas-Neira, J.L., Ayala, A. CXCL2 Polymorphism in Sepsis and ARDS: PathologicalSignificance Lost in Translation. Crit. Care Med. 25 (2007).

Lomas-Neira, J., Chung, C.S., Perl, M., Gregory, S., Biffl, W., Ayala, A. "Roleof alveolar macrophage & migrating neutrophils in hemorrhage induced priming forALI subsequent to septic challenge." Amer. J. Physiol. 290:51-58 (2006).

Lomas-Neira, J.L., Chung, C.S., Wesche, D.E., Perl, M., Ayala, A. "In vivo genesilencing (with siRNA) of pulmonary expression of MIP-2 vs. KC results in divergent effects on hemorrhage induced neutrophil mediated septic acute lung injury."J. Leukocyte Biol. 77:846-853 (2005).

Lomas-Neira, J.L., Chung, C.S., Wesche, D.E., Perl, M., Ayala, A. In vivo gen

e silencing (with siRNA) of pulmonary expression of MIP-2 vs. KC results in divergent effects on hemorrhage induced neutrophil mediated septic acute lung injury. J. Leukocyte Biol. 77:846-853 (2005).

Lomas-Neira, J., Ayala, A. Pepducins: an effective means to inhibit G-protein Coupled Receptor Signaling by neutrophils? Trends in Immunology. 26:619-621 (2005).

Lomas-Neira, J.L., Perl, M., Chung, C.S., Ayala, A. . Shock and hemorrhage: Anoverview of animal models. Shock 24 (Suplt. 1):33-39 (2005).

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CURRENT FUNDED PROJECTS"Mediators of endothelial cell dysfunction in the development of shock induced acute lung injury" Agency: The Rhode Island Foundation 3/25/10-3/24/11.

REPRESENTATIVE PRESENTATIONS

* "siRNA and the treatment of acute lung injury/sepsis." British Association forLung Research, British Inflammation Research Association & British PharmacologySociety Meeting. Girton College of Cambridge, London, United Kingdom (2006).

* Oral & Poster presentation in Session #43 (free communications) on 'Cellular Mechanism in Airway Inflammation', presentation entitled: "The Murine PulmonaryEndothelial Cell's Response to Pro-inflammatory Stimuli Associated with Traumatic Shock" at 7th International Congress on the Immune Consequences of Trauma, Shock and Sepsis, Munich, Germany, March 13-17, 2007

* Invited seminar/presentation entitled: "TNF-* Priming for the Development ofShock Induced Acute Lung Injury (ALI): Mediation by Local Tissue Not CirculatingCells" at Pfizer Pharmaceuticals, Inc. in Groton, CT, August 17, 2007

* Seminar speaker on topic entitled: TNF-* Priming for the Development of ShockInduced Acute Lung Injury (ALI): Mediation by Local Tissue Not Circulating Cells", as a part of Division of Surgical Research Seminar Series/Department of Surge

ry, held at the Rhode Island Hospital, Providence, RI, November 6th, 2007

* ASIP 2009 Experimental Pathologist-in-Training awardee. Presentation entitled: "Suppression of Angiopoietin-2 using in vivo siRNA following hemorrhagic shock ameliorates acute lung injury in murine shock/sepsis model", New Orleans, LA.