aspartylproteases in toxoplasma gondii and plasmodium ... · pdf filesarcocystis toxoplasma...
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Aspartyl proteasesinToxoplasma
gondii andPlasmodiumfalciparum
meBOP-ModuleII
DimaElSafadiImen MkadaAssef Hamdan
Apicomplexa
Piroplasmida
Haemosporida
Coccidia
Plasmodium spp
Theileriidiae
Babesiidae
Theileria
Babesia
Eimeriidae
Cryptosporidiidae
Sarcocystidae
EimeriidaEimeria
Cryptosporidium spp
Sarcocystis
Toxoplasma
Neospora
Malaria
Toxoplasmosis
cryptosporidiosis
East coast fever
Coccidiosis
Apicomplexans are human and animal pathogens
LifeCycleof Plasmodium– HumanCycle
LifeCycleof Toxoplasma – HumanCycle
Apicomplexan ASPs follow 6 distinct groups
TgASP3
PM1,2,4,HAPTgASP1
PMV
TgASP7
TgASP5
TgASP2
TgASP6
PMVII
PMVIII
PMVI
PMX
PMIX
TgASP4
PlasmodiumspecificHemoglobindegradation
D
C
BE
AF
ProteinexportPV/PVM
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?
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ASP3Knockdowninduciblesystem(Asp3-iKD)
• Intracellularparasites
• ASP3Knockout→lytic cycleofparasite(ASP3playanimportantroleinthelifecycle)
• ASP3Knockdowninduciblesystem(Asp3-iKD)
-ATC
Δku80
+ATC
Asp3-iKD
7days
PlaqueAssay
ASP3knockdowndisplaysaseverelyticcycledefect
Glidingmotility
Egress
Invasion
Intracellulargrowth
Attachment
CellLysis