asia europe chromatography all other enquiriesimg43.chem17.com/5/20100814/634173864745745345.pdf ·...

Thermo ScientificChromatography Columns and Consumables The right tools, separation after separation.

2010/2011}

2010201 1

Chromatography Colum

ns and Consumables

North America

USA and Canada800 332 3331865 354 4616 [email protected] Europe

France +33 (0) 3 88 67 53 20+33 (0) 3 88 67 11 68 [email protected] Germany+49 6103 408 0+49 6103 408 1111 [email protected]

Switzerland+41 56 618 41 11 +41 56 618 41 41 [email protected]

United Kingdom+44 1509 555500+44 1509 555111 [email protected]

Asia

Japan+81 45 453 9220+81 45 453 9226 [email protected]

China800-810-5118Shanghai: 86-21-64457830 faxBeijing: 86-1084193583 faxGuangzhou: 86-20-83486621 [email protected] India1800 22 8374 (toll-free)+91 22 6716 2200+91 22 6716 2244 [email protected]

All Other Enquiries

+44 (0) 1928 534 050+44 (0) 1928 534 049 [email protected]

© 2009 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.

CTGSCCHROMCAT 0110

For more information, please visit our website at www.thermoscientific.com/chromatography

How to Order

Resources for ChromatographersBeyond this catalog, our Chromatography team shares its extensive expertise through our web-based Chromatography Resource Center and the Separated by Experience enewsletter.

The Chromatography Resource Center, accessed at www.thermoscientific.com/chromatography provides technical support, applications, technical tips and literature to help you move your separations forward, quickly and easily.

The bi-monthly Separated by Experience enewsletter keeps you up-to-date on the latest technical and product information of interest to chromatographers. Valuable information developed by chromatographers for chromatographers. Subscribe today at www.thermoscientific.com/chromatography

Technical SupportNorth America800 332 [email protected]

Outside North America+44 (0) 1928 534 [email protected]

ThermoScientific_CVR_IFC_IBC_BC_FINAL.indd 1 12/7/2009 5:20:15 PM

This year’s catalog offers you 540 new pages of innovative and proven chromatography tools. It showcases our commitment of continued investment in all areas of chromatography to develop and expand our outstanding product portfolio using the most advanced materials and technologies to give you confidence, separation after separation. Where accuracy and repeatability are paramount for the delivery of consistently high quality results, we provide a wealth of chromatography consumables to ensure you have the right tools to meet all your separation needs.

With a long history of chromatography expertise and established products we now have grown our offering to include trusted names, such as Hypersil, Chromacol and National Scientific. This gives you choice, quality and dependability. Today, our comprehensive Thermo Scientific product portfolio continues to expand and includes many new and unique additions. With an extensive range of products, we place the broadest collection of chromatography consumables at your finger tips, including:

• Hypersil GOLD HPLC Columns • TraceGOLD and TracePLOT GC Columns• HyperSep Retain SPE Solutions• Reagents and accessories• National Scientific and Chromacol vials and closures tips.

In recognition of today’s stringent regulatory requirements, our investment extends to the quality assurance of our products. We can guarantee consistently accurate research tools. From R&D through to final production, utilizing our own global manufacturing and column packing facilities means our products are of the highest quality. Such is our commitment to chromatography consumables; we are currently expanding our cutting edge research and manufacturing facilities on a global scale.

This new Thermo Scientific Chromatography Columns and Consumables catalog is supported by our in-depth scientific applications and knowledge. Our expert technical team, in collaboration with many of our customers, is dedicated to constantly developing new techniques and troubleshooting guides to further improve method development and advance chromatography approaches in line with current and future analytical demands.

From sample preparation to separation and analysis, our tools help you achieve repeatable, predictable results – separation after separation. We hope that you find the information in this catalog helpful in your product selection and support.

In addition, we urge you to visit our website, www.thermoscientific.com/chromatography for additional information. Thank you for your continuing support of our product line.

Darren ThomasProduct Line Director, Chromatography Consumables

Welcome to the 2010 – 2011 Thermo Scientific Chromatography Columns and Consumables Catalog Notes

CCG_CVR_IFC_IBC_BC_ FINAL.indd 2 12/7/2009 5:11:13 PM

Sample Preparation ProductsOur comprehensive range of sample preparation products have been developed for rapid,

effective and economical sample preparation. Thermo Scientific HyperSep SPE products are

available in a range of formats including columns, multi-well plates and pipette tips. We also

offer the complete line of National Scientific syringes and the innovative WebSeal system.

86

536_Page 86-87.indd 86 12/10/2009 5:44:13 PM

creo

HyperSep RetainThermo Scientific HyperSep Retain is ideal for fast and easy sample preparation and method development.

QuEChERSThermo Scientific HyperSep Dispersive SPE products provide efficient sample preparation and clean-up using the QuEChERS Method.

Go to — PAGE 114

Go to — PAGE 94

trust

WebSealOur Chromacol WebSeal™ products are ideal for high throughput screening, combinational chemistry life science and HPLC.

Go to — PAGE 150

www.thermoscientific.com/chromatography 87

Sample Preparation

536_Page 86-87.indd 87 12/10/2009 5:44:33 PM

creo

SPE Method DevelopmentThe following flow chart briefly describes the common steps in SPE method development and optimization.

SPE Method Selection Overview

88

536_Page 88-89.indd 88 12/10/2009 5:46:54 PM

creo


SPE Method Development

536_Page 88-89.indd 89 12/10/2009 5:46:55 PM

creo

SPE Column Selection

SPE Phase Selection

Thermo Scientific HyperSep SPE products have been developed to meet the requirements of today’s sample preparation challenges. HyperSep SPE columns and multi-well plates are offered in a range of phases, ideal for use in application areas such as: Pharmaceutical, Biochemical, Environmental, and Food and Beverage.

We are committed to ensuring that high quality products are selected for your application. Please refer to the selection guide below to determine the correct product(s) for your particular application.

90

536_Page 90-91.indd 90 12/10/2009 6:01:56 PM

creo

SPE Phase Selection by Manufacturer Thermo Scientific HyperSep Product Alternative To

HyperSep C18, page 99 CLEAN-UP™ C18-U

Supelclean™ ENVI-18 / LC-18

SampliQ C18

BAKERBOND spe™ Polar Plus™

Isolute™ C18

CHROMABOND™ C18

Bond Elut™ C18

strata™ C18-U

Sep-Pak™ C18

HyperSep C8, page 100 CLEAN-UP C8

Supelclean ENVI-8 / LC-8

SampliQ C8

BAKERBOND spe Octyl C8

Isolute C8

CHROMABOND C8

Bond Elut C8

strata C8

Sep Pak C8

HyperSep Phenyl, page 101 CLEAN-UP Phenyl

Supelclean LC-Ph

SampliQ Phenyl

BAKERBOND spe Phenyl

Isolute Ph

Bond Elut Ph

strata Phenyl (PH)

HyperSep Silica, page 102 CLEAN-UP Silica

Supelclean LC-Si

SampliQ Silica

BAKERBOND spe Silica Gel

Isolute Silica

CHROMABOND SiOH

Bond Elut Si

strata Si-1

Sep Pak Si

HyperSep Aminopropyl, page 108 CLEAN-UP Aminopropyl

Supelclean LC-NH2 SampliQ Amino

BAKERBOND spe Amino

Isolute NH2 CHROMABOND NH2 Bond Elut NH2 strata NH2 Sep-Pak NH2

Thermo Scientific HyperSep Product Alternative To

HyperSep Florisil, page 107 CLEAN-UP Florisil

Supelclean ENVI-Florisil / LC-Florisil

SampliQ Florisil

BAKERBOND spe Florisil

Isolute Florisil

CHROMABOND Florisil

Bond Elut Florisil

strata FL-PR

Sep Pak Florisil

HyperSep SAX, page 103 CLEAN-UP QAX

Supelclean LC-SAX

SampliQ Si-SAX

BAKERBOND spe Quaternary amine

Isolute SAX

CHROMABOND SB

Bond Elut SAX

strata SAX

HyperSep SCX, page 104 CLEAN-UP BCX

Supelclean LC-SCX

SampliQ Si-SCX

BAKERBOND spe Aromatic Sulfonic Acid

Isolute SCX

CHROMABOND SA

Bond Elut SCX

strata SCX

HyperSep Verify-AX, page 106 CLEAN-UP THC

Isolute HAX

Bond Elut Certify II

strata Screen-A

HyperSep Verify-CX, page 105 CLEAN-UP DAU

Discovery DSC-MCAX

SampliQ C8/SCX

Isolute HCX

CHROMABOND Drug

Bond Elut Certify I

strata Screen-C

HyperSep Retain PEP, page 94 Oasis HLB (Waters)

Strata-X (Phenomenex)

FOCUS™ (Varian)

Easy (Macherey Nagel)

Isolute ENV+ (Biotage)

SampliQ OPT

StyreScreen DVB (UCT)

H2O-philic DVB (JT Baker)

HyperSep Retain-CX, page 96 Oasis MCX (Waters)

SampliQ SCX

Strata-X-C (Phenomenex)

StyreScreen DBX (UCT)

HyperSep Retain-AX, page 98 Oasis MAX (Waters)

SampliQ SAX

StyreScreen QAX (UCT)


SPE Column Selection

536_Page 90-91.indd 91 12/10/2009 6:01:56 PM

creo

eVol Dispensing SystemAllows precise and accurate, operator-independent dispensing for better deployment of laboratory staff

Applications:

•Routinedispensingofvolatilesolvents,hazardous,corrosiveorviscouschemicals

}Can be calibrated easily by user to ensure validity of results

}Intuitive interface with touch wheel and full-color screen

}Complies to GLP and GMP protocols

}Improves confidence in reported results

}Unifies two precision devices, a digitally controlled electronic drive and an XCHANGETM analytical syringe

}Integrated XCHANGETM coupling allows syringes to be quickly and easily changed

}XCHANGETM syringes offer exceptional versatility and functionality

}Addition of MEPS to XCHANGETM syringe offers automated sample preparation by SPE in a handheld system

92

HyperSep HypercarbUnique material for retention of highly polar compounds

}Flat, 100% porous graphitic carbon (PGC) with selectivity for structurally similar compounds, offering separation of compounds with simple solvents

}pH stable 0 to 14

}High batch-to-batch reproducibility

}Strong retention properties allow use of low bed weights for concentrated extracts

}Interaction mechanism with polar molecules

eVol Dispensing SystemType Volume Cat. No. Quantity SampleDispensingSystem N/A 66002-020 1EacheVolTMXCHANGEsyringe 5µL 66002-021 1EacheVolTMXCHANGEsyringe 50µL 66002-022 1EacheVolTMXCHANGEsyringe 500µL 66002-023 1EachSampleDispensingSystemKit N/A 66002-024 1EacheVolStand N/A 66002-025 1Each

HyperSep Hypercarb SPE ColumnsBed Weight Column Volume Cat. No. Quantity 25mg 1mL 60106-304 50Pack50mg 1mL 60106-303 50Pack100mg 1mL 60106-302 30Pack200mg 3mL 60106-301 30Pack500mg 6mL 60106-402 20Pack1g 6mL 60106-403 10Pack2g 15mL 60106-404 10Pack

HyperSep-96 Hypercarb Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60302-601 100Pack25mg 1mL 60302-602 100Pack50mg 1mL 60302-603 100PackWell Plates10mg 1mL 60302-606 1Each25mg 1mL 60302-607 1Each50mg 1mL 60302-608 1Each

536_2-93.indd 92 12/14/2009 11:03:43 AM

creo

93

Sample Preparation

}Flat, 100% porous graphitic carbon (PGC) with selectivity for structurally similar compounds, offering separation of compounds with simple solvents

}pH stable 0 to 14

}High batch-to-batch reproducibility

}Strong retention properties allow use of low bed weights for concentrated extracts

}Interaction mechanism with polar molecules

Cyanuric Acid in Drinking WaterCompounds: Cyanuric AcidPart Number: 60106-402Phase: HyperSep™ HypercarbVolume: 6mLBed Weight: 500mgSample Adjust water sample to pH 3 Pretreatment: Conditioning: Wash column with 10mL methanol Condition column with 10mL LC-grade waterApplication: Force/aspirate 250-500mL of water sample into column at rate

of 5mL/min.Washing: Dry column under vacuumElution: 20mL methanol – evaporate to dryness at 50°C under nitrogenSource: Marie Claire Hennion, ESPCI, Paris

Cyanuric Acid is an FDA-accepted component of feed-grade biuret, a ruminant feed additive. It is also found in swimming pool water a chlorine stabilizer. Consumer exposure may be through swallowing swimming pool water, through drinking water processed from surface water and through fish which may accumulate this chemical.

The loading capacity and the effects of drying out of material on recovery were investigated for HyperSep Hypercarb using a 200mg 3mL column (part number 60106-301) . Here, melamine was used to demonstrate Hypercarb’s ability to recover polar compounds.

• The Effect of Post-Loading or Washing Drying Time

The HyperSep Hypercarb SPE cartridges were dried after loading the analytes by drawing air through the packed bed. HyperSep Hypercarb gave a 95% recovery of melamine, irrespective of the drying time. (Figure 1)

• Loading CapacityThe loading capacity of HyperSep Hypercarb was established using an aqueous solution of melamine with a concentration equal to that of 1% of the sorbent bed weight in 1mL. (Figure 2) HyperSep Hypercarb was found to have a loading capacity of approximately 4% of the column bed weight.

Application Example for HyperSep Hypercarb – Cyanuric Acid in Drinking Water

Loading Capacity and the Effects of Drying Out of Hypercarb

Porous Graphitic Carbon (PGC) is a unique stationary phase composed of flat sheets of hexagonally arranged carbon atoms with a satisfied valence, as in a very large polynuclear aromatic molecule. PGC is unlike traditional silica bonded phases in both its structure and retentive properties, allowing for total pH stability and the retention and separation of highly polar species. HyperSep HypercarbTM is ideal for ‘problem’ analytes in SPE applications.

Thermo Scientific HyperSep Reversed Phase / Polar Retention Effect on Graphite Method (Hypercarb):

1. Analyte Properties – Polar, uncharged species – can also retain some ionic/charged species

2. Sample Preparation/Application (Polar, aqueous) – Add internal standard to the sample if quantification is desired. Optimize sample application by removing particulates if necessary (centrifugation or filtration) and/or diluting viscous matrices with water or buffer to ensure proper pH for desired interactions. This will require some experimentation.

3. Column Conditioning – 2-5 column volumes of a strong eluting solvent followed by 2-5 column volumes of water.

4. Column Wash – Use water or a weak eluting solvent which will wash most interferences from the sorbent without loss of analytes. Wash pH may greatly affect cleanup and/or recovery.

5. Elution – Use a strong eluting solvent to elute the analytes of interest. Addition of 0.1% TFA to the solvent will increase its elution strength or polar analytes.

Suggested Elution Solvents

• MeOH• THF*• Ethyl Acetate*• Dichloromethane*• Chloroform*

*Elution strength will depend on the nature of the analytes.

HyperSep Hypercarb SPE Columns

Increasing Elution Strength

(Figure 2)

(Figure 1)

www.thermoscientific.com/chromatography

536_Page 92-93.indd 93 12/14/2009 4:04:08 PM

creo

94

HyperSep Retain PEP

Results:

Compound Name HyperSep Retain PEP Recovery

Nicosulfuron 0.91

Thifensulfuron-methyl 0.89

Metsulfuron-methyl 0.89

Sulfometuron-methyl 0.86

Chlorsulfuron 0.99

Ethametsulfuron-methyl 0.81

Tribenuron 0.15

Bensulfuron-methyl 0.82

Pyrazosulfuron-ethyl 0.93

Chlorimuron-ethyl 1.07

Generic Procedure for HyperSep Retain PEP:

Benefits of using HyperSep Retain PEP

A comparison was made between HyperSep Retain PEP & HyperSep C18 SPE Cartridges to demonstrate the benefits of using a polymeric-based material for SPE applications. There are benefits to using HyperSep Retain polymeric SPE materials for SPE applica-tions. Here, a HyperSep Retain PEP 60mg 3mL column (part number 60107-203) was compared with a HyperSep C18 200mg 3mL column (part number 60108-303).

• The Effect of Post-Loading or Washing Drying Time

The SPE cartridges were dried after loading the analytes by drawing air through the packed bed:

1. ConDiTioning: Methanol followed by de-ionised water

2. SamPLE LoaDing: Load sample at a rate of 1-2mL/min.

3. WaShing: Methanol / de-ionised water (5/95, v/v)

4. ELuTion: Organic solvent such as methanol or acetonitrile

Application Example for HyperSep Retain PEP – Thiourea herbicides in Soil

1. SamPLE PRE-TREaTmEnT: Thiourea herbicides extracted from soil (1ppm) in phosphorate buffer solution (pH 2.5)

2. ConDiTioning: 5mL methanol followed by 5mL phosphorate buffer (pH 2.5)

3. SamPLE LoaDing: Load 10mL sample at 1-2mL /min.

4. WaShing: 3mL phosphorate buffer (pH 2.5)

5. ELuTion: 5mL acetonitrile / phosphorate buffer (pH 7.8) (9:1, v/v)After drying the HyperSep C18 bed for 1 minute, the recovery levels of both salicyclic acid and acetaminophen were significantly reduced. Acetaminophen showed a 20% decrease in recovery, with subsequent drying producing additional losses, eventually demonstrating recoveries of less than 30% after 10 minutes.

The experiment was repeated using the HyperSep Retain PEP sorbent and the acetaminophen recovery was sustained at > 95% irrespective of drying time.

The effect of drying time, measured using recovery levels of salicyclic acid and acetaminophen

• Loading Capacity

The loading capacities of the HyperSep Retain PEP and HyperSep C18 were compared using acetaminophen as the compound of interest.On the HyperSep C18 sorbent, compound breakthrough occurred when the sample load was less than 5% of the packed bed weight. However, HyperSep Retain PEP demonstrated a sample loading capacity up to 15% of the packed bed weight. This effect may be attributed to the higher surface area of the polymeric media. Consequently, the higher capacity of the polymeric material results in a reduced risk of compound breakthrough. A further advantage of the HyperSep Retain PEP over HyperSep C18 is that equal or better recovery can be obtained using a smaller bed weight, leading to smaller volumes of solvent, which has environmental and economic advantages.

Loading capacities of HyperSep Retain PEP (60mg) and HyperSep C18 (200mg) as measured using breakthrough of acetaminophen.

536_Page 94-95.indd 94 12/10/2009 6:05:42 PM

creo


Sample Preparation

HyperSep Retain PEPVersatile polymeric material for retention of polar and non-polar analytes

}Exceptional recoveries for polar and non-polar analytes

}high and consistent recoveries

}high capacity material is a high purity, highly porous polystyrene DVB material modified with urea functional groups to give balanced retention of polar and non-polar analytes

}Fast and easy sample preparation and method development

}ph stable 0 to 14

Applications:

•Drugsandmetabolitesin biologicalfluids

•Peptidesinserum,plasmaorbiologicalfluids

•Environmentalsamples

HyperSep Retain PEP SPE ColumnsBed Weight Column Volume Cat. No. Quantity 30mg 1mL 60107-201 100 Pack30mg 3mL 60107-202 50 Pack60mg 3mL 60107-203 50 Pack60mg 6mL 60107-208 30 Pack100mg 6mL 60107-207 30 Pack150mg 6mL 60107-211 30 Pack200mg 3mL 60107-204 50 Pack200mg 6mL 60107-212 30 Pack500mg 3mL 60107-205 50 Pack500mg 6mL 60107-206 30 Pack1g 25mL 60107-215 20 Pack2g 25mL 60107-214 20 Pack

hyperSep-96 Retain PEP Well Plates and individual WellsBed Weight Volume Cat. No. Quantity individual Wells5mg 1mL 60303-201 100 Pack10mg 1mL 60303-202 100 Pack30mg 1mL 60303-203 100 Pack60mg 1mL 60303-204 100 PackWell Plates5mg 1mL 60303-205 1 Each10mg 1mL 60303-206 1 Each30mg 1mL 60303-207 1 Each60mg 1mL 60303-208 1 Each

536_Page 94-95.indd 95 12/10/2009 6:05:49 PM

creo

96

HyperSep Retain-CXVersatile polymeric material for retention of basic compounds

}Exceptional recoveries for basic analytes

}High and consistent recoveries

}High capacity material is a high purity, highly porous polystyrene DVB material partially modified with sulfonic acid functional groups


}pH stable 0 to 14

Applications:

•Analysisofawiderangeofdrugsofabuseincludingbasicandneutraldrugs

HyperSep-96 Retain-CX Well Plates and Individual WellsBed Weight Column Volume Cat. No. QuantityIndividual Wells5mg 1mL 60303-301 100Pack10mg 1mL 60303-302 100Pack30mg 1mL 60303-303 100Pack60mg 1mL 60303-304 100PackWell Plates5mg 1mL 60303-305 1Each10mg 1mL 60303-306 1Each30mg 1mL 60303-307 1Each60mg 1mL 60303-308 1Each

HyperSep Retain CX SPE ColumnsBed Weight Column Volume Cat. No. Quantity30mg 1mL 60107-301 100Pack30mg 3mL 60107-302 50Pack60mg 3mL 60107-303 50Pack60mg 6mL 60107-308 30Pack100mg 6mL 60107-307 30Pack150mg 6mL 60107-311 30Pack200mg 3mL 60107-304 50Pack200mg 6mL 60107-314 30Pack500mg 3mL 60107-305 50Pack500mg 6mL 60107-306 30Pack1g 25mL 60107-315 20Pack2g 25mL 60107-312 20Pack

536_Page 96-97.indd 96 12/10/2009 6:07:12 PM

creo


Sample Preparation

Comparison – Acetaminophen in Calf Serum

HyperSep Retain-CX offers additional selectivity for basic compounds. A comparison of the recovery levels generated by HyperSep Retain PEP 60mg 3mL column (part number 60107-203) and HyperSep Retain-CX 60mg 3mL column (part number 60107-303) were compared. The compound analysed was acetaminophen from calf serum.

1. SAMPLE PRE-TREATMENT: Dilute serum with 10mmol phosphoric acid of the same volume

2. CONDITIONING: 2mL methanol followed by 2mL DI Water

3. SAMPLE LOADING: Load 2mL sample

4. WASHING: 2mL methanol / water (5/95, v/v)

5. ELUTION: 2mL methanol

HyperSep Retain-CX gave additional selectivity for a basic compound such as acetaminophen when compared with HyperSep Retain PEP.

HyperSep Retain-CX Method / Application Example / Retain-CX vs Retain PEP for basic compounds

SPE Column Recovery

HyperSepRetainPEP 86.9%

HyperSepRetain-CX 94.6%

Generic Procedure for HyperSep Retain-CX:

1. CONDITIONING: Methanol followed by de-ionised water

2. SAMPLE LOADING: Load sample at a rate of 1-2mL/min.

3. WASHING: Methanol / de-ionised water (5/95, v/v)

4. ELUTION: Organic solvent such as methanol or acetonitrile

Application Example – Melamine in Animal Feeds

PartNumber: 60107-303Phase: HyperSepRetain-CXVolume: 3mLBedWeight: 60mgSample To5gofanimalfeed,add50mLof0.1%trichloroaceticacidPretreatment: aqueoussolution.Vortexfor1min,thenadd2mLof2%lead acetateaqueoussolution.Sonicatefor20mins,thentransfer aportionofthemixturetoa10mLCentrifugetube.Centrifuge at8000rpmfor10mins.Conditioning: 3mLmethanolfollowedby3mLdeionizedwaterApplication: Load3mLsampleat1-2mL/minWashing: 3mLwaterfollowedby3mLmethanol.PurgecolumntodrynessElution: 5mLammoniainmethanol(5:95,v/v).Drysampleundernitrogen stream.Reconstitutewith20%methanolaqueoussolution.

536_Page 96-97.indd 97 12/10/2009 6:07:13 PM

creo

98

Generic Procedure for HyperSep Retain-AX:1. ConditioninG: Methanol followed by de-ionised water2. SAmPle loAdinG: Load sample at a rate of 1-2mL/min.3. WASHinG: Water containing 2% ammonium hydroxide4. elution: Methanol containing 2% acetic acid

HyperSep Retain-AXVersatile polymeric material for retention of acidic compounds

}exceptional recoveries for acidic analytes

}High and consistent recoveries

}High capacity material is a high purity, highly porous polystyrene dVB material partially modified with quaternary amine functional groups


}pH stable 0 to 14

Applications:

• Analysis of THC and its metabolites

HyperSep-96 Retain-AX Well Plates and individual WellsBed Weight Volume Cat. no. Quantityindividual Wells5mg 1mL 60303-401 100 Pack10mg 1mL 60303-402 100 Pack30mg 1mL 60303-403 100 Pack60mg 1mL 60303-404 100 PackWell Plates5mg 1mL 60303-405 1 Each10mg 1mL 60303-406 1 Each30mg 1mL 60303-407 1 Each60mg 1mL 60303-408 1 Each

HyperSep Retain AX SPe ColumnsBed Weight Column Volume Cat. no. Quantity30mg 1mL 60107-401 100 Pack30mg 3mL 60107-402 50 Pack60mg 3mL 60107-403 50 Pack60mg 6mL 60107-408 30 Pack100mg 6mL 60107-407 30 Pack150mg 6mL 60107-411 30 Pack200mg 3mL 60107-404 50 Pack200mg 6mL 60107-412 30 Pack500mg 3mL 60107-405 50 Pack500mg 6mL 60107-406 30 Pack1g 25mL 60107-415 20 Pack2g 25mL 60107-414 20 Pack

Compounds: Methylmalonic acid (MMA)Part Number: 60107-403Phase: HyperSep Retain-AXVolume: 3mLBed Weight: 60mgSample To 250μL serum or plasma add 100μL Pretreatment: D3MMA (2.5μM/L) and 1.5mL waterConditioning 2mL methanol followed by 2mL waterApplication: Load samples at 1-2mL/min.Washing: 2mL water followed by 2mL methanol with 0.5% acetic acidElution: 2mL Tert Butyl Methyl Ether (TBME) with 3% formic acid. Evaporate to dryness at <40ºC.Derivatization: Add 50μL acetonitrile and 25μL MTBSTFA + 1% TBDMCl. Heat at 70ºC for 10 mins.Source: Department of Laboratory Medicine, The Hospital of Telemark

Compounds: NadifloxacinPart Number: 60107-403Phase: HyperSep Retain-AXVolume: 3mLBed Weight: 60mgSample 5ppm of nadifloxacin sample in Pretreatment: 50mM phosphate pH 7.4Conditioning: 1mL methanol followed by 1mL of 2M NaOH and 1mL deionized waterApplication: Load 5mL sample at 1-2mL/min.Washing: 1mL of 5% ammonia aqueous solution followed by 1mL methanolElution: 3mL of methanol with 4% acetic acid

Application example – methylmalonic Acid

Application example – nadifloxacin

536_Page 98-99.indd 98 12/10/2009 6:08:49 PM

creo


Sample Preparation

}Retentive for most non-polar compounds

}Retains most organic analytes from aqueous matrices

}Hydrophobic reversed phase

}For nonpolar to moderately polar compounds

HyperSep C18Feature a highly retentive alkyl-bonded phase for non-polar to moderately polar compounds

Applications:

• Retentive for non-polar compounds

• Retains most organic analytes from aqueous matrices

HyperSep C18 SPe ColumnsBed Weight Column Volume Cat. no. Quantity50mg 1mL 60108-390 100 Pack100mg 1mL 60108-302 100 Pack200mg 3mL 60108-303 50 Pack500mg 3mL 60108-304 50 Pack500mg 6mL 60108-305 30 Pack1g 6mL 60108-301 30 Pack2g 15mL 60108-701 20 Pack5g 25mL 60108-702 20 Pack10g 75mL 60108-703 10 Pack

HyperSep-96 C18 Well Plates and individual WellsBed Weight Volume Cat. no. Quantity individual Wells10mg 1mL 60300-421 100 Pack25mg 1mL 60300-422 100 Pack50mg 1mL 60300-423 100 Pack100mg 1mL 60300-524 100 PackWell Plates10mg 1mL 60300-425 1 Each25mg 1mL 60300-426 1 Each50mg 1mL 60300-427 1 Each100mg 1mL 60300-428 1 Each

536_Page 98-99.indd 99 12/10/2009 6:08:58 PM

creo

100

HyperSep C8Less retentive alternative to C18 for polar and non-polar compounds

}Well-suited for methods requiring less retention than C18

}Excellent for moderately polar analytes

}Potential for polar interactions greater than with C18

Applications:

• Drugs and their metabolites in biological samples

• Peptides in biological samples

HyperSep C8 SPE ColumnsBed Weight Column Volume Cat. No. Quantity50mg 1mL 60108-391 100 Pack100mg 1mL 60108-392 100 Pack200mg 3mL 60108-393 50 Pack500mg 3mL 60108-309 50 Pack500mg 6mL 60108-394 30 Pack1g 6mL 60108-427 30 Pack2g 15mL 60108-704 20 Pack5g 25mL 60108-705 20 Pack10g 75mL 60108-706 10 Pack

HyperSep-96 C8 Well Plates and Individual WellsBed Weight Volume Cat. No. Quantity Individual Wells10mg 1mL 60300-441 100 Pack25mg 1mL 60300-442 100 Pack50mg 1mL 60300-443 100 Pack100mg 1mL 60300-444 100 PackWell Plates10mg 1mL 60300-445 1 Each25mg 1mL 60300-446 1 Each50mg 1mL 60300-447 1 Each100mg 1mL 60300-448 1 Each

536_Page 100-101.indd 100 12/10/2009 6:10:24 PM

creo


Sample Preparation

HyperSep PhenylOffer alternative selectivity for retention of basic compounds

}Commonly employed for nonpolar interactions

}The electron density of the aromatic ring provides different selectivity than other non-polar sorbents

Applications:

• Extraction of aromatic compounds

• Extraction of basic analytes

HyperSep Phenyl SPE ColumnsBed Weight Column Volume Cat. No. Quantity50mg 1mL 60108-516 100 Pack100mg 1mL 60108-386 100 Pack200mg 3mL 60108-387 50 Pack500mg 3mL 60108-388 50 Pack500mg 6mL 60108-389 30 Pack1g 6mL 60108-517 30 Pack2g 15mL 60108-707 20 Pack5g 25mL 60108-708 20 Pack10g 75mL 60108-709 10 Pack

HyperSep-96 Phenyl Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-681 100 Pack25mg 1mL 60300-682 100 Pack50mg 1mL 60300-683 100 Pack100mg 1mL 60300-684 100 PackWell Plates10mg 1mL 60300-685 1 Each25mg 1mL 60300-686 1 Each50mg 1mL 60300-687 1 Each100mg 1mL 60300-688 1 Each

536_Page 100-101.indd 101 12/10/2009 6:10:33 PM

creo

102

HyperSep SilicaA polar sorbent primarily used to retain analytes in nonpolar matrices

}Effectively separates compounds of very similar structure

}Adsorbs analytes from nonpolar solvents like hydrocarbons, less polar esters and ethers

}Suitable for use as an intermediate strength cation exchanger in aqueous medium

Applications:

•Extractionofpolarcompoundsincludingaldehydes,amines,drugs,pesticidesandherbicides

•Extractionofcarotenoids,fat-solublevitamins,aflatoxinsinfoodmatrices

•Extractionoffattyacidsandphospholipids

HyperSep Silica SPE ColumnsBed weight Column Volume Cat. No. Quantity50mg 1mL 60108-409 100Pack100mg 1mL 60108-317 100Pack200mg 3mL 60108-410 50Pack500mg 3mL 60108-315 50Pack500mg 6mL 60108-411 30Pack1g 6mL 60108-426 30Pack2g 15mL 60108-710 20Pack5g 25mL 60108-711 20Pack10g 75mL 60108-712 10Pack

HyperSep-96 Silica Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-481 100Pack25mg 1mL 60300-482 100Pack50mg 1mL 60300-483 100Pack100mg 1mL 60300-484 100PackWell Plates10mg 1mL 60300-485 1Each25mg 1mL 60300-486 1Each50mg 1mL 60300-487 1Each100mg 1mL 60300-488 1Each

536_Page 102-103.indd 102 12/10/2009 6:12:24 PM

creo


Sample Preparation

HyperSep SAX Strong Anion ExchangerStrong anion exchange sorbent for extraction of weak acids

}Extracts negatively charged compounds from both aqueous and non-aqueous solutions

}Ideally suited for the extraction of weak acids, e.g. carboxylic acids

}Amine group masks the effect of the carbon chain of the functional group

}Selectivity can be tuned by selection of buffer

Applications:

•Isolationofanionicproteins

•Removalofacidicfoodpigments

•Isolationofphenoliccompounds

•Nucleicacids,nucleotidesandsurfactants

Applications:

•Extractionofantibiotics,drugs,organicbases,aminoacids,catecholaminesandherbicides

HyperSep SAX SPE ColumnsBed Weight Column Volume Cat. No. Quantity50mg 1mL 60108-418 100Pack100mg 1mL 60108-360 100Pack200mg 3mL 60108-417 50Pack500mg 3mL 60108-419 50Pack500mg 6mL 60108-434 30Pack1g 6mL 60108-521 30Pack2g 15mL 60108-713 20Pack5g 25mL 60108-714 20Pack10g 75mL 60108-715 10Pack

HyperSep-96 SAX Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-561 100Pack25mg 1mL 60300-562 100Pack50mg 1mL 60300-563 100Pack100mg 1mL 60300-564 100PackWell Plates10mg 1mL 60300-565 1Each25mg 1mL 60300-566 1Each50mg 1mL 60300-567 1Each100mg 1mL 60300-568 1Each

536_Page 102-103.indd 103 12/10/2009 6:12:33 PM

creo

104

Applications:

•Isolationofanionicproteins

•Removalofacidicfoodpigments

•Isolationofphenoliccompounds

•Nucleicacids,nucleotidesandsurfactants

HyperSep SCX Strong Cation ExchangerA strong cation exchange sorbent for extraction of charged basic compounds

}Benzene ring provides potential for non-polar interactions

}Low pKa value sorbent

HyperSep-96 SCX Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-581 100Pack25mg 1mL 60300-582 100Pack50mg 1mL 60300-583 100Pack100mg 1mL 60300-584 100PackWell Plates10mg 1mL 60300-585 1Each25mg 1mL 60300-586 1Each50mg 1mL 60300-587 1Each100mg 1mL 60300-588 1Each

HyperSep SCX SPE ColumnsBed Weight Column Volume Cat. No. Quantity50mg 1mL 60108-420 100Pack100mg 1mL 60108-421 100Pack200mg 3mL 60108-422 50Pack500mg 3mL 60108-423 50Pack500mg 6mL 60108-520 30Pack1g 6mL 60108-433 30Pack2g 15mL 60108-716 20Pack5g 25mL 60108-717 20Pack10g 75mL 60108-718 10Pack

536_Page 104-105.indd 104 12/10/2009 6:13:59 PM

creo


Sample Preparation

HyperSep Verify-CXFeatures non-polar and anionic characteristics for improved analysis of drugs of abuse

}Mixed mode sorbent for extraction of basic drugs

}Separation based on two functional groups: reversed phase C8 and an ion exchanger, benzene sulfonic acid

}Co-polymerized on a rigid, purified silica gel support

Applications:

•Analysisofawiderangeofdrugsofabusefrombiologicalmatrices,includingbasicandneutraldrugs

HyperSep-96 Verify-CX Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-801 100Pack25mg 1mL 60300-802 100Pack50mg 1mL 60300-803 100Pack100mg 1mL 60300-804 100PackWell Plates10mg 1mL 60300-805 1Each25mg 1mL 60300-806 1Each50mg 1mL 60300-807 1Each100mg 1mL 60300-808 1Each

HyperSep Verify-CX SPE ColumnsBed Weight Column Volume Cat. No. Quantity130mg 1mL 60108-719 100Pack200mg 6mL 60108-722 50Pack300mg 3mL 60108-720 50Pack500mg 3mL 60108-721 50Pack500mg 6mL 60108-723 30Pack1g 6mL 60108-724 30Pack

536_Page 104-105.indd 105 12/10/2009 6:14:01 PM

creo

106

HyperSep Verify-AXFeatures nonpolar and cationic characteristics for improved analysis of acidic drugs and metabolites

}Mixed mode sorbent suited for the extraction of acidic drugs and metabolites from biological matrices

}Separation based on two functional groups: reversed phase C8 and an ion exchanger, quaternary amine

}Rigid, purified silica gel support to which the two functionalities are co-polymerized

Applications:

•AnalysisofTHCanditsmetabolites

HyperSep Verify-AX SPE ColumnsBed Weight Column Volume Cat. No. Quantity130mg 1mL 60108-727 100Pack200mg 6mL 60108-730 50Pack300mg 3mL 60108-728 50Pack500mg 3mL 60108-729 50Pack500mg 6mL 60108-731 30Pack1g 6mL 60108-732 30Pack

HyperSep-96 Verify-AX Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-809 100Pack25mg 1mL 60300-810 100Pack50mg 1mL 60300-811 100Pack100mg 1mL 60300-812 100PackWell Plates10mg 1mL 60300-813 1Each25mg 1mL 60300-814 1Each50mg 1mL 60300-815 1Each100mg 1mL 60300-816 1Each

536_Page 106-107.indd 106 12/10/2009 6:15:18 PM

creo


Sample Preparation

HyperSep FlorisilIdeal for the isolation of polar compounds from non-polar matrices

}Magnesia-loaded silica gel offers rapid flowrates for large sample volumes

}Extremely polar in nature, especially well-suited for separation of chlorinated pesticides

Applications:

•Extractionofpesticidesusingofficialmethods

•Polychlorinatedbiphenylsintransformeroil

•Alcohol,aldehydes,aminesanddrugs

HyperSep Florisil SPE ColumnsBed Weight Column Volume Cat. No. Quantity50mg 1mL 60108-402 100Pack100mg 1mL 60108-403 100Pack200mg 3mL 60108-404 50Pack500mg 3mL 60108-405 50Pack500mg 6mL 60108-500 30Pack1g 6mL 60108-431 30Pack2g 15mL 60108-735 20Pack5g 25mL 60108-736 20Pack10g 75mL 60108-737 10Pack

HyperSep-96 Florisil Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-721 100Pack25mg 1mL 60300-722 100Pack50mg 1mL 60300-723 100Pack100mg 1mL 60300-724 100PackWell Plates10mg 1mL 60300-725 1Each25mg 1mL 60300-726 1Each50mg 1mL 60300-727 1Each100mg 1mL 60300-728 1Each

536_Page 106-107.indd 107 12/10/2009 6:15:29 PM

creo

108

HyperSep AminopropylA polar sorbent for both polar and anion exchange interactions

}Weaker anion exchanger than sorbents like SAX

}Enhanced retention of strong anions like sulfonic acids

}Excellent retention of drugs, metabolites and structural isomer

Applications:

•Separationofstructuralisomers

•Drugsandmetabolitesinbiologicalfluids

•Separationofsaccharides,phenolsandpetroleumproducts

HyperSep Aminopropyl SPE ColumnsBed Weight Column Volume Cat. No. Quantity50mg 1mL 60108-424 100Pack100mg 1mL 60108-364 100Pack200mg 3mL 60108-425 50Pack500mg 3mL 60108-518 50Pack500mg 6mL 60108-519 30Pack1g 6mL 60108-432 30Pack2g 15mL 60108-738 20Pack5g 25mL 60108-739 20Pack10g 75mL 60108-740 10Pack

HyperSep-96 Aminopropyl Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-501 100Pack25mg 1mL 60300-502 100Pack50mg 1mL 60300-503 100Pack100mg 1mL 60300-504 100PackWell Plates10mg 1mL 60300-505 1Each25mg 1mL 60300-506 1Each50mg 1mL 60300-507 1Each100mg 1mL 60300-508 1Each

536_Page 108-109.indd 108 12/10/2009 6:16:58 PM

creo


Sample Preparation

109

HyperSep CyanoOptimized for the retention of polar compounds from non-polar matrices

}Medium polarity sorbent, less retentive than silica or diol

}Suitable for reverse phase extractions of moderately polar compounds

Applications:

•Retainingpolarcompoundsfromhexaneandoils

•Reversedphaseextractionofmoderatelypolarcompounds

HyperSep-96 Cyano Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-817 100Pack25mg 1mL 60300-818 100Pack50mg 1mL 60300-819 100Pack100mg 1mL 60300-820 100PackWell Plates10mg 1mL 60300-821 1Each25mg 1mL 60300-822 1Each50mg 1mL 60300-823 1Each100mg 1mL 60300-824 1Each

HyperSep Cyano SPE ColumnsBed Weight Column Volume Cat. No. Quantity50mg 1mL 60108-746 100Pack100mg 1mL 60108-745 100Pack200mg 3mL 60108-747 50Pack500mg 3mL 60108-748 50Pack500mg 6mL 60108-749 30Pack1g 6mL 60108-750 30Pack2g 15mL 60108-751 20Pack5g 25mL 60108-752 20Pack10g 75mL 60108-753 10Pack

536_Page 108-109.indd 109 12/10/2009 6:17:06 PM

creo

110

}Short alkyl chains with polar functional groupsApplications:

•Normalphaseextractionofpolarcompounds

•Purificationofpolarcompounds

HyperSep DiolIdeal for the extraction of polar compounds

HyperSep-96 Diol SPE ColumnsBed Weight Column Volume Cat. No. Quantity50mg 1mL 60108-571 100Pack100mg 1mL 60108-572 100Pack200mg 3mL 60108-573 50Pack500mg 3mL 60108-574 50Pack500mg 6mL 60108-575 30Pack1g 6mL 60108-576 30Pack2g 15mL 60108-755 20Pack5g 25mL 60108-756 20Pack10g 75mL 60108-757 10Pack

HyperSep-96 Diol Well Plates and Individual WellsBed Weight Volume Cat. No. QuantityIndividual Wells10mg 1mL 60300-635 100Pack25mg 1mL 60300-636 100Pack50mg 1mL 60300-637 100Pack100mg 1mL 60300-638 100PackWell Plates10mg 1mL 60300-630 1Each25mg 1mL 60300-631 1Each50mg 1mL 60300-632 1Each100mg 1mL 60300-633 1Each

536_Page 110-111.indd 110 12/10/2009 6:19:18 PM

creo


Sample Preparation

111

HyperSep Universal Vacuum Manifold

Universal Vacuum Manifold

Universal ManifoldBase/Gauge

}Accommodates both SPE columns and 96-well plates

System supplied with: Manifold, base/gauge, flask & stopper, tubing & spigots

HyperSep Universal Vacuum ManifoldDescription Cat. No. QuantityUniversalVacuumManifold 60104-230 1EachVacuumPump,NorthAmericanPlug 60104-241 1EachVacuumPump,EuropeanPlug 60104-243 1EachPlugsfor24-positionExtractionPlate 60104-234 24PackPlugsfor48-positionExtractionPlate 60104-235 48Pack

536_Page 110-111.indd 111 12/10/2009 6:19:29 PM

creo

112

HyperSep Glass Block Manifolds

Manifold Lid

Collection Rack

Manifold Safety Tray

Vacuum Gauge and Valve Assembly

}16-Port Vacuum Manifold:

Glass Block, Corian Manifold Lid, Cover Gasket, Vacuum Gauge and Valve Assembly, 16 Teflon Tips, Adjustable Collection Rack, Bulkhead Luer Fittings, 16 Plugs and Manifold Safety Tray

}24-Port Vacuum Manifold:

Glass Block, Corian Manifold Lid, Cover Gasket, Vacuum Gauge and Valve Assembly, 24 Teflon Tips, Adjustable Collection Rack, Bulkhead Luer Fittings, 24 Plugs and Manifold Safety Tray

Luer Lock Plugs Retaining Clips for Collection Rack

Bulkhead Luer Fittings Vacuum Gauge and Valve Assembly

Stopcocks Teflon Tips

Manifold Lid Legs

HyperSep Glass Block ManifoldsDescription Cat. No. Quantity16-port Vacuum Manifold 60104-232 1 Each24-port Vacuum Manifold 60104-233 1 EachVacuum Pump, North American Plug 60104-241 1 EachVacuum Pump, European Plug 60104-243 1 EachReplacement PartsVacuum Gauge 60104-240 1 EachStopcocks for 16-port Vacuum Manifold 60104-242 16 PackStopcocks for 24-port Vacuum Manifold 60104-244 24 PackTFE tips for Vacuum Manifold 60104-245 12 PackVacuum Gauge and Valve Assembly 60104-261 1 EachLid for 16-port Glass Block Manifold 60104-262 1 EachLid for 24-port Glass Block Manifold 60104-248 1 EachGasket for 16-port Manifold 60104-249 1 EachGasket for 24-port Manifold 60104-250 1 EachCollection Rack for 16-port Vacuum Manifold 60104-251 1 EachCollection Rack for 24-port Vacuum Manifold 60104-252 1 EachGlass Block for 16-port Vacuum Manifold 60104-253 1 EachGlass Block for 24-port Vacuum Manifold 60104-254 1 EachManifold Safety Tray 60104-260 1 EachRetaining Clips for Collection Rack 60104-255 12 PackBulkhead Luer Fittings 60104-256 12 PackManifold Lid Legs 60104-257 4 PackLuer Lock Plugs 60104-258 12 Pack

536_Page 112-113.indd 112 12/10/2009 6:21:48 PM

creo


Sample Preparation

HyperSep SPE AccessoriesA range of accessories to complement the HyperSep SPE Manifolds

HyperSep-96 Well Plate Manifold

HyperSep-96 Well Plate

Manifold Lid

Manifold Base

Included with system:}Base

}Lid

}Waste collection tray

HyperSep-96 well plate is not included and needs to be purchased separately

HyperSep SPE AccessoriesDescription Cat. No. Quantity Base Plate for HyperSep-96 Well Plate 60300-301 1 EachBase Plate for HyperSep-96 Well Plate 60300-303 5 PackSample Collection Plate, 1mL 60300-402 50 PackSample Collection Plate, 2mL 60300-403 50 PackAdaptors for 1mL, 3mL and 6mL SPE Columns 60104-259 15 PackEmpty 1mL Wells 60300-311 100 PackEmpty 1mL Wells, Fritted 60300-318 100 Pack

HyperSep-96 Well Plate ManifoldsDescription Cat. No. Quantity HyperSep-96 Vacuum Manifold 60103-351 1 EachVacuum Pump, North American Plug 60104-241 1 EachVacuum Pump, European Plug 60104-243 1 Each

536_Page 112-113.indd 113 12/10/2009 6:22:12 PM

creo

114

QuEChERS Dispersive SPE Product SelectionThe QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) technique is increasingly becoming the technique of choice for extraction and clean-up of pesticide residues. It was developed by the USDA Eastern Regional Research Centre. Please refer to the information below for selection of the most appropriate QuEChERS product for multi-residue pesticide analysis:

There are four variations of the QuEChERS method currently being used:

• TheoriginalQuEChERSmethod.Thiswasintroducedin2003,usingsodium chloride to enhance extraction

• DispersiveAOAC2007.01.Thisusessodiumacetateasabuffer,replacing sodium chloride

• Thedualphasevariation–thismethodintroducestheuseofPSAandGCB to remove high levels of chlorophyll and plant sterols in the final extract without the loss of planar pesticides (polar aromatics) using anacetone:toluenesolventmix(3:1)

• TheEuropeanversionissimilartotheAOACmethod,excepttheextraction uses sodium chloride, sodium citrate dihydrate and disodium citrate sesquihydrate instead of sodium acetate

Sorbents used in QuEChERS Methods:

Material Purpose Typical Matrices

MgSO4 Removal of excess water Fruits, vegetables

PSA (Primary/Secondary Amine) Removal of organic acids, Fruits fatty acids, sugars

C18 Removal of lipids & sterols Milk, meat, fish

GCB (Graphatized Carbon Black) Removal of pigments & sterols Wine, green vegetables, carrots

Product Selection:

Matrix Type Examples Sorbent Requirements

General Matrices Apples MgSO4, PSA Cucumber MelonFatty Matrices Milk MgSO4, PSA, C18 Cereals FishPigmented Matrices Lettuce MgSO4, PSA, C18, GCB Carrot WineHigh Pigmented Matrices Spinach MgSO4, PSA, C18, GCB Red Peppers

Method Part Number Description

Original 60105-211 50mL Tube with 4g MgSO4, 1g Sodium Chloride 250pk

AOAC 2007.1 60105-210 50mL Tube with 6g MgSO4, 1.5g Sodium Acetate 250pk

60105-203 2mL Tube with 150mg MgSO4, 50mg PSA 100pk

60105-204 2mL Tube with 150mg MgSO4, 50mg PSA, 50mg C18 100pk

60105-205 15mL Tube with 900mg MgSO4, 300mg PSA, 150mg GCB 50pk


60105-223 2mL Tube with 150mg MgSO4, 50mg PSA, 50mg C18, 50mg GCB 100pk



60105-226 15mL Tube with 1200mg MgSO4, 400mg PSA, 400mg C18, 400mg GCB 50pk

European 60105-212 50mL Tube with 6g MgSO4, 1.5g NaCl, 1.5g Sodium EN15662 Citrate Tribasic Dihydrate, 0.75g Sodium Citrate Dibasic Sesquihydrate

60105-216 50mL Tube with 4g MgSO4, 1g NaCl, 1g Sodium Citrate Tribasic Dihydrate, 0.5g Sodium Citrate Dibasic Sesquihydrate

60105-215 15mL Tube with 900mg MgSO4, 150mg PSA, 50pk




60105-221 2mL Tube with 150mg MgSO4, 25mg PSA, 2.5mg GCB 100pk

60105-222 2mL Tube with 150mg MgSO4, 25mg PSA, 7.5mg GCB 100pk


Dual Phase 60105-207 6mL Column with 200mg GCB on Top, 400mg PSA Method on Bottom, separated by a Frit 30pk

60105-208 6mL Column with 250mg GCB on Top, 500mg PSA on Bottom, separated by a Frit 30pk

60105-209 6mL Column with 500mg GCB on Top, 500mg PSA on Bottom, separated by a Frit 30pk

Other variations of QuEChERS are available – please see page 115 for full ordering information

536_Page 114-115.indd 114 12/10/2009 6:23:37 PM

creo


Sample Preparation

QuEChERS ProductsConvenient and effective approach for determining pesticide residues in fruit, vegetables and other foods

The QuEChERS method is a two-step process: extraction followed by cleanup. TheextractionstepproductsuseMgSO4 to aid extraction, along with either NaCl sodium citrate, or anhydrous sodium acetate for “base-sensitive” compounds (e.g., folpetorcaptan).Theextractionstepproductsaresuppliedina50mLpolypropylenecentrifuge tube for convenient extractions. The clean-up step products contain PSA (primary/secondary amine) for the removal of organic acids and polar pigments among other compounds. Some products couple the PSA with endcapped C18 for the removalof most lipids and sterols, or graphitized carbon black for the removal of sterols and pigments such as chlorophyll. A variety of tube formats and bed weights are available to accommodate large and small sample sizes.

}Determine greater number of pesticides than with standard SPE

}Easy to use

}Available in a number of configurations

QuEChERS Extraction ProductsDescription Capacity Cat. No. Quantity 6g anhydrous MgSO4, 1.5g anhydrous sodium acetate 50mL 60105-210 250 Pack4g anhydrous MgSO4, 1g anhydrous NaCl 50mL 60105-211 250 Pack4g anhydrous MgSO4, 1g sodium chloride, 1g sodium citrate dihydrate, 0.5g disodium citrate sesquihydrate 50mL 60105-216 250 Pack6g anhydrous MgSO4, 1.5g sodium chloride, 1.5g sodium citrate dihydrate, 0.75g disodium citrate sesquihydrate 50mL 60105-212 250 Pack

QuEChERS Clean-Up ProductsDescription Capacity Cat. No. Quantity 150mg anhydrous MgSO4, 25mg PSA 2mL 60105-219 100 Pack150mg anhydrous MgSO4, 25mg PSA, 25mg C18 2mL 60105-220 100 Pack150mg anhydrous MgSO4, 25mg PSA, 2.5mg graphitized carbon 2mL 60105-221 100 Pack150mg anhydrous MgSO4, 25mg PSA, 7.5mg graphitized carbon 2mL 60105-222 100 Pack150mg anhydrous MgSO4, 50mg PSA, 50mg graphitized carbon 2mL 60105-202 100 Pack150mg anhydrous MgSO4, 50mg PSA 2mL 60105-203 100 Pack150mg anhydrous MgSO4, 50mg PSA, 50mg endcapped C18 2mL 60105-204 100 Pack150mg anhydrous MgSO4,50mg PSA, 50mg endcapped C18, 50mg graphitized carbon 2mL 60105-223 100 Pack900mg anhydrous MgSO4, 300mg PSA, 150mg endcapped C18 15mL 60105-206 50 Pack900mg anhydrous MgSO4, 300mg PSA, 150mg graphitized carbon 15mL 60105-205 50 Pack1200mg anhydrous MgSO4,400mg PSA 15mL 60105-224 50 Pack1200mg anhydrous MgSO4,400mg PSA, 400mg endcapped C18 15mL 60105-225 50 Pack1200mg anhydrous MgSO4,400mg PSA, 400mg endcapped C18, 400mg graphitized carbon 15mL 60105-226 50 Pack900mg anhydrous MgSO4, 150mg PSA, 150mg C18 15mL 60105-227 50 Pack150mg anhydrous MgSO4,50mg PSA, 50mg graphitized carbon 15mL 60105-230 50 Pack900mg anhydrous MgSO4,150mg PSA, 45mg graphitized carbon 15mL 60105-217 50 Pack900mg anhydrous MgSO4,150mg PSA, 15mg graphitized carbon 15mL 60105-218 50 Pack900mg anhydrous MgSO4,150mg PSA 15mL 60105-215 50 Pack150mg anhydrous MgSO4,300mg PSA, 150mg ChloroFiltr 15mL 60105-231 50 Pack750mg anhydrous MgSO4, 250mg PSA, 250mg endcapped C18, 250mg graphitized carbon 15mL 60105-213 50 Pack900mg anhydrous MgSO4, 300mg PSA 15mL 60105-214 50 Pack400mg PSA on bottom, 200mg graphitized carbon on top, separated by a Teflon frit 6mL 60105-207 30 Pack500mg PSA on bottom, 250mg graphitized carbon on top, separated by a Teflon frit 6mL 60105-208 30 Pack500mg PSA on bottom, 500mg graphitized carbon on top, separated by a Teflon frit 6mL 60105-209 30 Pack

ChloroFiltr is a trademark of United Chemical Technologies Inc.

536_Page 114-115.indd 115 12/10/2009 6:23:42 PM

creo

116

For non-base sensitive compounds using the European EN15662 method}Weigh 15g of homogenized (hydrated at least 80%) sample in a 50mL centrifuge tube

}Add 15mL acetonitrile (or 1:1 acetone/hexane, ethyl acetate) and IS

}Shake briefly

}Add 6g anhydrous magnesium sulfate, 1.5g sodium chloride, 1.5g sodium citrate Tribadic dehydrate, 0.75g sodium citrate dibasic

}Shake by hand for 1 minute

}Centrifuge at 5,000 rpm for 5 minutes

}Transfer a portion of supernatant to a QuEChERS clean up tube

}Shake for 30 seconds

}Centrifuge for 1 minute at 6,000 rpm

For non-base sensitive compounds, such as bendiocarb and diuron using the original QuEChERS method}Add 15mL of acetonitrile to QuEChERS centrifuge tube

}Shake to mix contents

}Add surrogate or internal standards if necessary

}Add 15g of homogenised hydrated sample and shake for 1 minute

}Centrifuge tube for 1 minute at 3700rcf

}Add an aliquot of the supernatant to the appropriate clean-up tube (and shake for 1 minute)

}Centrifuge for 1 minute at 3700rcf

}Analyze extract

For base sensitive compounds such as folpet and captan using the AOAC 2007.01 QuEChERS method}Add 15mL of 1% acetic acid in acetonitrile to QuEChERS centrifuge tube

}Shake to mix contents

}Add surrogate or internal standards if necessary

}Add 15g of homogenised hydrated sample and shake for 1 minute

}Centrifuge tube for 1 minute at 3700rcf

}Add an aliquot of the supernatant to the appropriate clean-up tube and shake for 1 minute

}Centrifuge for 1 minute at 3700rcf

}Analyze extract

For polar aromatic (planar) compounds such as matrix plant pigments using the Schenck method}Pre-rinse the cartridge with 5mL of toluene

}Add an aliquot of the supernatant to the cartridge

}Start collection

}Elute with 6-12mL of 3:1 acetone:toluene

}Concentrate for GC/MS analysis - or -

}Concentrate to dryness and reconstitute in mobile phase for LC analysis

QuEChERS Methods

536_Page 116-117.indd 116 12/10/2009 6:24:41 PM

creo


Sample Preparation

QuEChERS Dispersive SPE Technical InformationConsiderations in Method Development:

1) Determine the properties of the pesticides of interest:

a. Base sensitive b. pH dependent c. Non-base sensitive

Product Selection —Sample Extraction:

• BaseSensitiveCompounds Useextractionproductwithsodiumacetate • Non-BaseSensitiveCompounds Useextractionproductwithsodiumchlorideorsodiumcitrate

2)Determinethepropertiesofthesamplematrix:

• General • Fatty • Pigmented • HighlyPigmented

Product Selection —Sample Cleanup:

MatrixType Examples SorbentRequirements

General Matrices Apples MgSO4, PSA Cucumber MelonFatty Matrices Milk MgSO4, PSA, C18 Cereals FishPigmented Matrices Lettuce MgSO4, PSA, C18, GCB Carrot WineHigh Pigmented Matrices Spinach MgSO4, PSA, C18, GCB Red Peppers

QuEChERS Troubleshooting:

Problem Causes RecommendedSolutions

Loss of planar Presence of GCB may result Use a product with less GCB pesticides in a loss of planar compounds Use the Dual Phase QuEChERS product

Loss of acidic Presence of PSA will extract Use a product containing compounds e.g. 2,4-D acidic compounds from matrix MgSO4 and C18 from starting matrix

Loss of compounds Some compounds are Use an ‘analyte protectant e.g. toluene during subsequent unstable and can break down or sorbitol analysis during analysis

Addition of sample to Exothermic reaction between Add the sample to the tube, then theQuEChERS Extraction water in sample and MgSO4 solvents, then the sorbent materialstube containing sorbent causes an exothermic reaction

Poor recovery of Sample not in appropriate Wrong products used in methodpesticide compounds homogenisation state Ensure sample is hydrated to 80% or higher Verify nature of pesticides e.g. are base sensitive compounds present

536_Page 116-117.indd 117 12/10/2009 6:24:45 PM

creo

118

Aspire Protein A and Protein G TipsPurify antibodies in minutes

}Capture and purify a wide range of monoclonal and polyclonal IgG antibodies from serum, ascites and cell culture supernatants

}Embedded with high quality immobilized Thermo Scientific Protein A Plus and Protein G Plus Agarose with the capacity to purity (1mg of human IgG

}Spin column capacity for a fraction of the cost

}Fast and easy protocol does not compromise purity and yield

Aspire RP30 Desalting TipsEffectively remove ion-suppressing contaminants from digested complex protein mixtures for LC/MS and LC/MS/MS analysis within 20 minutes

Proprietary reversed-phase resin allows superior peptide binding and recovery of digested complex protein mixtures compared to conventional C18 products.

}Removes salts, detergents and other ion-suppressing contaminants

}Reduced ion-suppression, increase signal-to-noise ratios and sequence coverage

}Effective sample cleanup prior to LC/MS and LC/MS/MS analysis

}20-Minute purification protocol features color-coded parallel sample processing

}Bind up to 30µg of total peptide without the need for centrifugation

Aspire IMAC Cobalt TipsQuick and clean his-tagged protein purification

}Design in a pipette tip format to effectively purify up to 0.5mg of his-tagged proteins from cell protein lysates

}Embedded with Thermo Scientific HisPur Cobalt Resin with higher specificity compared to conventional nickel resins

}Spin column capacity for a fraction of the cost per sample

}Color-coded, multichannel Thermo Scientific Aspire protocol allows parallel sample processing in minutes without the need for centrifugation

Aspire Protein A and Protein G TipsDescription Cat. No. Quantity Aspire Protein A Tips 990-03 96 PackAspire Protein G Tips 990-02 96 Pack

Aspire IMAC Cobalt TipsDescription Cat. No. Quantity Aspire IMAC Cobalt Tips 990-04 96 Pack

Aspire RP30 Desalting TipsDescription Cat. No. Quantity Aspire RP30 Desalting Tips 990-01 96 Pack

536_Page 118-119.indd 118 12/10/2009 6:26:28 PM

creo


Sample Preparation

HyperSep Microscale Solid Phase Extraction TipsRevolutionary micropipette tip sample preparation

}Faster sample preparation with minimal sample loss

}Patented micropipet tip in which the chromatographic material is directly attached to its inner surface

}No contamination from the supporting matrix

}Separation in volumes as low as 100nL

Applications:

•Massspectrometry

•Desalting

•Proteinpurification

•MALDI

•Electrophoresis

•HPCE

•HPLC

•CEC

HyperSep Tip Microscale Solid Phase Extraction TipsMaterial Cat. No. Quantity1-10µL CapacityBioBasic C18 60109-201 96 PackBioBasic C8 60109-202 96 PackBioBasic C4 60109-203 96 PackHypercarb 60109-204 96 PackHypercarbandC18(mixmode) 60109-205 96 PackHILIC 60109-206 96 PackTrypsin 60109-207 96 PackTitaniumDioxide 60109-208 96 PackZirconiumDioxide 60109-217 96 Pack10-200µL CapacityBioBasic C18 60109-209 96 PackBioBasic C8 60109-210 96 PackBioBasic C4 60109-211 96 PackHypercarb 60109-212 96 PackHypercarbandC18(MixMode) 60109-213 96 PackHILIC 60109-214 96 PackTrypsin 60109-215 96 PackTitaniumDioxide 60109-216 96 PackZirconiumDioxide 60109-218 96 Pack

536_Page 118-119.indd 119 12/10/2009 6:26:34 PM

creo

120

HyperSep SpinTip Microscale Solid Phase Extraction TipsRevolutionary micropipette tip for sample preparation

}Pipet tips with a 1 to 2µm wide slit at bottom that permits the liquid to pass through but retains the chromatographic material (20 to 30µm)

}Faster sample preparation with minimal sample loss

}No contamination from the supporting matrix

}Separation in volumes as low as 100nL

HyperSep SpinTip Microscale Solid Phase Extraction TipsMaterial Cat. No. Quantity1-10µL CapacityC18 60109-401 96 PackC8 60109-402 96 PackC4 60109-403 96 PackHypercarb 60109-404 96 PackHypercarb and C18 (mix mode) 60109-405 96 PackHILIC 60109-406 96 PackTrypsin 60109-407 96 PackPOROS Weak Anion Exchanger 60109-408 96 PackPOROS Strong Anion Exchanger 60109-409 96 PackPOROS Strong Cation Exchanger 60109-410 96 PackTitanium Dioxide 60109-411 96 PackZirconium Dioxide 60109-424 96 Pack10-200µL CapacityC18 60109-412 96 PackC8 60109-413 96 PackC4 60109-414 96 PackHypercarb 60109-415 96 PackHypercarb and C18 (mix mode) 60109-416 96 PackHILIC 60109-417 96 PackTrypsin 60109-418 96 PackPOROS Weak Anion Exchanger 60109-419 96 PackPOROS Strong Anion Exchanger 60109-420 96 PackPOROS Strong Cation Exchanger 60109-421 96 PackTitanium Dioxide 60109-422 96 PackZirconium Dioxide 60109-425 96 Pack

536_Page 120-121.indd 120 12/10/2009 6:27:49 PM

creo


Sample Preparation

HyperSep Lab PlatesFor the purification and sample preparation of proteins, DNA, RNA and other biomolecules

}Sample concentration of small-scale samples

}Available in a range of chromatographic materials

}96-Well format with media embedded at the bottom of the plate

}Can be processed manually or using a liquid-handling robot

}Not suitable for use with a vacuum

Applications:

•Tissuecultureandseparation of products

•Sampleconcentration

•Samplecleanup

•Collectionofsampleafter chromatography

HyperSep Lab PlatesDescription Cat. No. QuantityPolystyreneC18 60110-201 5 PackC8 60110-202 5 PackC4 60110-203 5 PackHypercarb 60110-204 5 PackHypercarb and C18 (Mixed Mode) 60110-205 5 PackZirconium Dioxide 60110-206 5 PackTitanium Dioxide 60110-207 5 PackSCX 60110-208 5 PackSAX 60110-209 5 PackPolypropyleneC18 60110-301 5 PackC8 60110-302 5 PackC4 60110-303 5 PackHypercarb 60110-304 5 PackHypercarb and C18 (Mixed Mode) 60110-305 5 PackZirconium Dioxide 60110-306 5 PackTitanium Dioxide 60110-307 5 PackSCX 60110-308 5 PackSAX 60110-309 5 Pack

536_Page 120-121.indd 121 12/10/2009 6:27:57 PM

creo

122

HyperSep Filter PlatesFor effective cleanup of small-scale samples

}96-Well plate for the purification and separation of proteins, peptides, DNA, RNA and other biomolecules

}Cleanup of microgram-level samples

}Can be used under vacuum

}Available in a range of chromatographic materials

HyperSep Filter PlatesDescription Cat. No. Quantity5-7µL Bed VolumeC18 60110-401 1 EachC8 60110-402 1 EachC4 60110-403 1 EachHypercarb 60110-404 1 EachHypercarb and C18 (Mix Mode) 60110-405 1 EachZiconium Dioxide 60110-406 1 EachTitanium Dioxide 60110-407 1 EachSCX 60110-408 1 EachSAX 60110-409 1 Each40µL Bed VolumeC18 60110-501 1 EachC8 60110-502 1 EachC4 60110-503 1 EachHypercarb 60110-504 1 EachHypercarb and C18 (Mix Mode) 60110-505 1 EachZirconium Dioxide 60110-506 1 EachTitanium Dioxide 60110-507 1 EachSCX 60110-508 1 EachSAX 60110-509 1 Each

536_Page 122-123.indd 122 12/10/2009 6:29:15 PM

creo


Sample Preparation

HyperSep MEPS ProductsOnline SPE for GC and LC sample preparation − extraction to injection in a single process

Save Hours in Sample Preparation}Reduce the time to prepare and inject samples from hours to minutes

}Eliminate all extra steps between sample preparation and sample injection

}Reduce buffer and solvent volume from Milliliters to Microliters

}Reduce the sample volume needed to as little as 3.6µL

What is MEPS?MEPS is Micro Extraction by Packed Sorbent and is a new development in the fields of sample preparation and sample handling. MEPS is the miniaturization of conventional SPE packed bed devices from milliliter bed volumes to microliter volumes.

The MEPS approach to sample preparation is suitable for reversed phases, normal phases, mixed mode or ion exchange chemistries. MEPS is available in a variety of common SPE phases.

The MEPS Barrel Insert and Needle Assembly contains the stationary phase, and is built into the syringe needle.

Why use MEPS?Historically, many sample preparation methods used liquid-liquid extraction (LLE) which required large volumes of sample, solvents and time. The advantages of SPE over LLE are that SPE takes much less time, can be developed into a fully automated technique, requires much less solvent and offers selectivity.

MEPS performs the same functions as SPE – the removal of interfering matrix components and the selective isolation and concentration of analytes. MEPS increases the advantages of conventional SPE in the following ways:

}Significantly reduces the time needed to prepare and inject samples

}Can be combined with LC or GC automation – the extraction step and injection step are performed on-line using the same syringe.

}Significantly reduces the volume of solvents needed

}Ability to work with samples as small as 3.6µL versus several hundred mL for SPE

Sample Size and SensitivitySample volumes may be as little as 10µL, or by taking multiple aliquots of 100µL or 250µL, samples of 1mL or larger may be concentrated.

AutomationThe capability to extract samples and make injections on-line using a single device reduces both sample processing times and the need for operator intervention.

Sorbent LifeTypical BIN life for extraction of whole plasma sample is conservatively about 40 to 100 samples. This significantly increases for cleaner samples.

CarryoverThe small quantity of phase in the MEPS products can be easily and effectively washed between samples to reduce the possibility of carryover. This washing process is simply not practical with off-line SPE devices. With automation of MEPS, washing can occur while the previous sample is running.

Flexible and easy to useThe dimensions of the sorbent bed ensure that the performance remains identical to conventional SPE devices when used for extraction of similar samples. MEPS products can be used for sample volumes as small as 3.6µL making them particularly well suited to on-line use with LC-MS analysis of volume limited samples.

536_Page 122-123.indd 123 12/14/2009 4:21:32 PM

creo

124

HyperSep MEPS for GC Applications: Thermo Scientific, CTC Analytics, HTA and Varian 8400 Systems

}For use with 100 and 250µL MEPS syringes

HyperSep MEPS Syringe Components

HyperSep MEPS for GC Applications: CTC Analytics Using 250µL Syringes

HyperSep MEPS Development Kit for GC applications contains 1 each of Retain PEP, Retain-CX, Retain-AX, Hypercarb and C18

HyperSep MEPS Development Kit for GC applications contains 1 each of Retain PEP, Retain-CX, Retain-AX, Hypercarb and C18

MEPS Syringes and ComponentsDescription Cat. No. QuantityThermo Scientific, CTC analytics, HTA and Varian 8400 systems100µL removable needle MEPS syringe 60308-101 1 EachReplacement plunger assembly for 100µL MEPS syringe 60308-102 1 Each250µL Removable Needle MEPS Syringe 60308-103 1 EachReplacement plunger assembly for 250µL MEPS syringe 60308-104 1 EachCTC Analytics Only250µL removable needle MEPS syringe 60308-105 1 EachReplacement plunger assembly for 250µL CTC-compatible syringe 60308-106 1 Each

MEPS For GC : Thermo Scientific, CTC Analytics, HTA and Varian 8400 SystemsDescription Cat. No. QuantityHyperSep Retain PEP MEPS 60308-201 5 PackHyperSep Retain-CX MEPS 60308-202 5 PackHyperSep Retain-AX MEPS 60308-203 5 PackHyperSep Hypercarb MEPS 60308-204 5 PackHyperSep Verify-CX MEPS 60308-205 5 PackHyperSep Verify-AX MEPS 60308-206 5 PackHyperSep C18 MEPS 60308-207 5 PackHyperSep Silica MEPS 60308-208 5 PackMEPS Development Kit for GC Applications 60308-209 5 Pack

MEPS For GC: CTC Analytics using 250µL SyringesDescription Cat. No. QuantityHyperSep Retain PEP MEPS 60308-301 5 PackHyperSep Retain-CX MEPS 60308-302 5 PackHyperSep Retain-AX MEPS 60308-303 5 PackHyperSep Hypercarb MEPS 60308-304 5 PackHyperSep Verify-CX MEPS 60308-305 5 PackHyperSep Verify-AX MEPS 60308-306 5 PackHyperSep C18 MEPS 60308-307 5 PackHyperSep Silica MEPS 60308-308 5 PackMEPS Development Kit for GC Applications 60308-309 5 Pack

536_Page 124-125.indd 124 12/10/2009 6:30:40 PM

creo


Sample Preparation

HyperSep MEPS For LC Applications: Thermo Scientific, CTC Analytics, HTA and Varian 8400 Systems

}For use with 100 and 250µL MEPS syringes

HyperSep MEPS Development Kit for LC applications contains 1 each of Retain PEP, Retain-CX, Retain-AX, Hypercarb and C18.

HyperSep MEPS For LC Applications: CTC Analytics Using 250uL Syringes

}For use with 250µL MEPS syringes

HyperSep MEPS Development Kit for LC applications contains 1 each of Retain PEP, Retain-CX, Retain-AX, Hypercarb and C18.

HyperSep Protein Precipitation PlateRemoval of proteins using the CRASH method

}Dual frit design

}Hydrophobic/oleophobic frits to enable only precipitation of proteins

}Pore size optimized for ideal flowrate

}Specially selected polypropylene for low extractables

MEPS For LC:Thermo Scientific, CTC Analytics, HTA and Varian 8400 SystemsDescription Cat. No. QuantityHyperSep Retain PEP MEPS 60308-401 5 PackHyperSep Retain-CX MEPS 60308-402 5 PackHyperSep Retain-AX MEPS 60308-403 5 PackHyperSep Hypercarb MEPS 60308-404 5 PackHyperSep Verify-CX MEPS 60308-405 5 PackHyperSep Verify-AX MEPS 60308-406 5 PackHyperSep C18 MEPS 60308-407 5 PackHyperSep Silica MEPS 60308-408 5 PackMEPS Development Kit for LC applications 60308-409 5 Pack

MEPS For LC Applications: CTC Analytics using 250µL SyringesDescription Cat. No. QuantityHyperSep Retain PEP MEPS 60308-501 5 PackHyperSep Retain-CX MEPS 60308-502 5 PackHyperSep Retain-AX MEPS 60308-503 5 PackHyperSep Hypercarb MEPS 60308-504 5 PackHyperSep Verify-CX MEPS 60308-505 5 PackHyperSep Verify-AX MEPS 60308-506 5 PackHyperSep C18 MEPS 60308-507 5 PackHyperSep Silica MEPS 60308-508 5 PackHyperSep MEPS Development Kit for LC applications 60308-509 5 Pack

Protein Precipitation ProductsDescription Cat. No. QuantityProtein Precipitation Plate 60304-201 1 Each

536_Page 124-125.indd 125 12/10/2009 6:30:41 PM

creo

126

HyperSep Online SPE ProductsRetain specific analytes in a sample matrix when used with an appropriate HPLC column

}Effective removal of contaminants such as proteins from samples

}Compatible with conventional HPLC systems

}Fast and effective clean-up and concentration of target compounds

}HyperSep Retain PEP: for retention of polar and non-polar analytes

}HyperSep Retain-CX: for retention of basic and non-polar analytes

}HyperSep Retain-AX: for retention of acidic and non-polar analytes

}HyperSep Hypercarb: for retention of extremely polar analytes

HyperSep Javelin Direct-Connect Online SPE ColumnsI.D. Length Retain-PEP Retain-CX Retain-AX Hypercarb Quantity2.1mm 10mm 60310-201 60310-301 60310-401 60310-501 4 Pack3.0mm 10mm 60310-202 60310-302 60310-402 60310-502 4 Pack

HyperSep UNIGUARD Direct-Connect Online SPE CartridgesI.D. Length Retain-PEP Retain-CX Retain-AX Hypercarb Quantity2.1mm 10mm 60311-201 60311-301 60311-401 60311-501 4 Pack3.0mm 10mm 60311-202 60311-302 60311-402 60311-502 4 Pack

HyperSep HPLC Columns for Online SPEI.D. Length Retain-PEP Retain-CX Retain-AX Hypercarb Quantity2.1mm 20mm 60312-201 60312-301 60312-401 60312-501 1 Each3.0mm 20mm 60312-202 60312-302 60312-402 60312-502 1 Each

This diagram shows the typical load and elution positions for the HyperSep Online SPE Setup

536_Page 126-127.indd 126 12/10/2009 6:32:46 PM

creo


Sample Preparation

Doxepin in Rat SerumCompounds: DoxepinPart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample Mix 10mL of doxepin aqueous Pretreatment: solution (20mg/L) and 30mL rat serum in a 100mL flask and dilute to volume with 0.5% ammonia solution: 2ppmConditioning: 2mL methanol, followed by 2mL deionized waterApplication: Load sample at 1-2mL/min.Washing: 2mL 0.5% ammonia solution containing 5% methanolElution: 2mL 1% acetic acid/methanol

HyperSep Retain PEP

Propranolol in Rat SerumCompounds: PropranololPart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample Mix 10 mL of propranolol aqueous Pretreatment: solution (100mg/L) and 30mL rat serum in a 100mL flask and dilute to volume with 0.5% ammonia solution: 10ppmConditioning: 2mL methanol, followed by 2mL deionized waterApplication: Load sample at 1-2mL/min.Washing: 2mL 0.5% ammonia solution containing 5% methanolElution: 2mL 1% acetic acid/methanol Phenols in Tap Water

Compounds: Phenol, 4-nitrophenol, m-methylphenol, 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, pentachlorophenolPart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample To a 0.5-1 ppm sample of phenols in Pretreatment: tap water add formic acid (1%)Conditioning: 2mL methanol followed by 2mL of 1% formic acidApplication: Load 10mL of sample at 1-2mL/min.Washing: 1mL of 1% formic acidElution: 2mL methanol. Dry sample under nitrogen and reconstitute using 1mL methanol/1% formic acid (1:1).

Acetaminophen in Calf SerumCompounds: AcetaminophenPart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample Dilute 10 mg acetaminophen in Pretreatment: 100mL water (100 ppm). Mix 25mL of this solution with 25 mL water. In a separate vessel, mix 25mL solution with 25mL serum/1% H3PO4.Conditioning: 2mL methanol followed by 2mL waterApplication: Load 2mL samplesWashing: 2mL methanol/water (5:95, v/v)Elution: 2mL methanol

Nitro AnilinesCompounds: 2-nitroaniline, 4-nitroanilinePart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgConditioning: 3mL methanol, followed by 3mL deionized waterApplication: Load 3mL of sample at 1-2mL/min.Washing: 1mL of 0.1% ammonia aqueous solutionElution: 3mL methanol/1% formic acid (95:5, v/v). Dry sample under nitrogen and reconstitute using 1mL methanol.

Aniline and N,N-dimethylaniline in Dionized WaterCompounds: Aniline, N,N-dimethylanilinePart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgConditioning: 3mL methanol, followed by 3mL deionized waterApplication: Load 3mL of sample at 1-2mL/min.Washing: 1mL of waterElution: 3mL methanol/water (95:5, v/v). Dry sample under nitrogen and reconstitute using 1mL methanol.

Thiourea Herbicides Extracted from Soil in Phosphorate Buffer Solution (pH 2.5)Compounds: Nicosulfuron, thifensulfuron-methyl, metsulfuron-methyl, sulfometuron- methyl, chlor-sulfuron, ethametsulfron methyl, tribenuron, bensulfuron- methyl, pyrazosulfuron-ethyl, chlorimuron-ethylPart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample Add thiourea herbicides extracted Pretreatment: from soil (1ppm) to phosphorate buffer solution (pH 2.5)Conditioning: 5mL methanol followed by 5mL phosphorate buffer (pH 2.5)Application: Load 10mL of sample at 1-2mL/min.Washing: 3mL of phosphorate buffer (pH 2.5)Elution: 5mL ACN/phosphorate buffer (pH 7.8) (9:1, v/v). Dry sample under nitrogen and reconstitute using 1mL methanol.

Atrazine in Deionized WaterCompounds: AtrazinePart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample 10 ppm atrazine in acetic acid (0.2%) Pretreatment: aqueous solutionConditioning: 2mL methanol followed by 2mL deionized waterApplication: Load 4 mL of 10 ppm atrazine in acetic acid (0.2%) aqueous solution sample at 1-2mL/min.Washing: 1mL of waterElution: 5mL methanol. Dry sample under nitrogen and reconstitute using 0.5mL water.

AcephateCompounds: AcephatePart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample 10ppm acephate in ammonium sulfate Pretreatment: aqueous solution (20% w/w)Conditioning: 2mL methanol followed by 2mL deionized waterApplication: Load 4mL of 10 ppm acephate in ammonium sulfate aqueous solution (20% w/w) at 1-2mL/min.Washing: 1mL of waterElution: 5mL methanol. Dry sample under nitrogen and reconstitute using 0.5mL water.

536_Page 126-127.indd 127 12/10/2009 6:32:47 PM

creo

PraziquantelCompounds: PraziquantelPart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample 20ppm praziquantel in 0.2% acetic Pretreatment: acid and 5% methanolConditioning: 2mL methanol followed by 2mL deionized waterApplication: Load 4mL of 20 ppm praziquatel in 0.2% acetic acid and 5% methanol at 1-2mL/min.Washing: Methanol/deionized water (5:95, v/v)Elution: 4mL methanol. Dry sample under nitrogen and reconstitute using 0.5mL water.

AcetaminophenCompounds: AcetaminophenPart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample 5ppm of acetaminophen in aqueous Pretreatment: solutioncontaining 0.2% ammonium acetate, pH 5Conditioning: 2mL methanol followed by 2mL deionized waterApplication: Load 4 mL of sample at 1-2mL/min.Washing: 1mL deionized waterElution: 5mL of methanol. Dry sample under nitrogen and reconstitute using water.

CefoperazoneCompounds: CefoperazonePart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample 5ppm Cefoperazone solution in water Pretreatment: Conditioning: 2mL methanol followed by 2mL deionized waterApplication: Load 10mL of 5 ppm cefoperazone solution in water at 1-2mL/min.Washing: 1mL of methanol/deionized water (5:95, v/v). Dry column under vacuum.Elution: 6mL methanol. Dry sample under nitrogen and reconstitute using 0.5mL water.

LovastatinCompounds: LovastatinPart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample 2ppm Lovastatin in 10% acetonitrile Pretreatment: aqueous solutionConditioning: 2mL methanol followed by 2mL deionized waterApplication: Load 10 mL of sample at 1-2mL/min. Dry column under vacuum.Washing: 1mL of deionized waterElution: 4mL of acetonitrile. Dry sample under nitrogen and reconstitute using 1mL methanol.

HyperSep Retain PEP

AcephateCompounds: AcephatePart Number: 60107-203Phase: HyperSep Retain PEPVolume: 3mLBed Weight: 60mgSample 10ppm acephate in ammonium sulfate Pretreatment: aqueous solution (20% w/w)Conditioning: 2mL methanol followed by 2mL deionized waterApplication: Load 4mL of 10 ppm acephate in ammonium sulfate aqueous solution (20% w/w) at 1-2mL/min.Washing: 1mL of waterElution: 5mL methanol. Dry sample under nitrogen and reconstitute using 0.5mL water.

128

536_Page 128-129.indd 128 12/10/2009 6:33:58 PM

creo

HyperSep Retain-CX

Acetaminophen in Calf SerumCompounds: AcetaminophenPart Number: 60107-303Phase: HyperSep Retain-CXVolume: 3mLBed Weight: 60mgSample Dilute 10 mg of acetaminophen to Pretreatment: 100 mL with water (100 ppm). Mix 25mL of acetaminophen (100 ppm) solution and 25mL serum/1% H3PO4 in a 50mL flask.Conditioning: 2mL methanol, followed by 2mL deionized waterApplication: Load samples at 1-2 mL/min.Washing: 2mL 5% methanol/waterElution: Elute acetaminophen with 2mL methanol

ß-Agonists in Pig LiverCompounds: Cimaterol, sulfamonomethoxine, clenbuterol hydrochloride, salbutamolPart Number: 60107-305Phase: HyperSep Retain-CXVolume: 3mLBed Weight: 500mgSample Extract 20g of pig liver sample using Pretreatment: ACN, then dry and spike with a standard of chloric acid (10 mmol) solution containing the 4 agonist compounds of interest to get sample solutions at 5 different concentrations (1μg/mL, 2μg/mL, 5μg/mL,10μg/mL and 100μg/mL)Conditioning: 5mL methanol, followed by 5mL deionized water and 5mL 30mmol/L chloric acidApplication: Load sample at 1-2mL/min.Washing: 5mL deionized water followed by 5mL methanol. Dry column under vacuumElution: 5mL of methanol containing 4% ammonia. Evaporate to dryness at 50°C using a nitrogen stream.

Melamine in Animal FeedsPart Number: 60107-303Phase: HyperSep Retain-CXVolume: 3mLBed Weight: 60mgSample To 5g of animal feed, add 50mL of Pretreatment: 0.1% trichloroacetic acid aqueous solution. Vortex for 1 min, then add 2mL of 2% lead acetate aqueous solution. Sonicate for 20 mins, then transfer a portion of the mixture to a 10mL Centrifuge tube. Centrifuge at 8000 rpm for 10 mins.Conditioning: 3mL methanol followed by 3mL deionized waterApplication: Load 3mL sample at 1-2 mL/min.Washing: 3mL water followed by 3mL methanol. Purge column to dryness.Elution: 5 mL ammonia in methanol (5:95, v/v). Dry sample under nitrogen stream. Reconstitute with 20% methanol aqueous solution.

ClenbuterolCompounds: ClenbuterolPart Number: 60107-303Phase: HyperSep Retain-CXVolume: 3mLBed Weight: 60mgSample 10ppm of clenbuterol in 20 mM Pretreatment: ammonia acetateConditioning: 2mL methanol followed by 2mL deionized water and 2mL 30 mM HClApplication: Load 1mL sampleWashing: 1mL of methanol followed by 1mL deionized waterElution: 2mL methanol with 4% ammonia. Dry sample under nitrogen and reconstitute using 1mL methanol/0.05% phosphoric acid (40:60, v/v).

SalbutamolCompounds: SalbutamolPart Number: 60107-303Phase: HyperSep Retain-CXVolume: 3mLBed Weight: 60mgSample 10ppm of salbutamol in 20 mM Pretreatment: ammonia acetateConditioning: 2mL methanol followed by 2mL deionized water and 2mL 30 mM HClApplication: Load 1mL sampleWashing: 1mL of methanol followed by 1mL deionized waterElution: 2mL methanol with 4% ammonia. Dry sample under nitrogen and reconstitute using 1mL methanol/0.05% phosphoric acid (40:60, v/v).

Melamine in Milk Products for HPLC AnalysisCompounds: MelaminePart Number: 60107-303Phase: HyperSep Retain-CXVolume: 3mLBed Weight: 60mgSample Weigh 5g of milk powder (or measure Pretreatment: 10mL of milk) into a 250mL flask. Add 50mL of 1% Trichloroacetic acid (TCA). Mix / vortex. Add 2mL of 2% lead acetate / water solution into the mixture then sonicate for 20 minutes. Transfer part of the final mixture into a 10mL centrifuge tube. Centrifuge for 10 minutes at 8,000 rpm.Conditioning 3mL methanol followed by 3mL DI waterApplication: Load 6mL sample at 1-2mL/min.Washing: 3mL DI water followed by 3mL methanol. Dry column (5 minutes at > 10 inches Hg)Elution: 5mL 5% ammonia / methanol Collect eluate at 1 to 2 mL/min. Evaporate to dryness at <50ºC using nitrogen. Reconstitute sample using 1mL of mobile phase.


Sample Preparation

536_Page 128-129.indd 129 12/10/2009 6:33:58 PM

creo

HyperSep Retain-AXNadifloxacinCompounds: NadifloxacinPart Number: 60107-403Phase: HyperSep Retain-AXVolume: 3mLBed Weight: 60mgSample 5ppm of nadifloxacin sample in Pretreatment: 50mM phosphate pH 7.4Conditioning: 1mL methanol followed by 1mL of 2M NaOH and 1mL deionized waterApplication: Load 5mL sample at 1-2mL/min.Washing: 1mL of 5% ammonia aqueous solution followed by 1mL methanolElution: 3mL of methanol with 4% acetic acid

NicosulfuronCompounds: NicosulfuronPart Number: 60107-403Phase: HyperSep Retain-AXVolume: 3mLBed Weight: 60mgSample 10ppm of nicosulfuron in Pretreatment: deionized waterConditioning: 1mL methanol followed by 1mL deionized waterApplication: Load 2mL sampleWashing: 1mL of 2% ammonia hydroxide followed by 1mL methanolElution: 2mL methanol with 2% acetic acid

Ethametsulfuron in Aqueous SolutionCompounds: EthametsulfuronPart Number: 60107-403Phase: HyperSep Retain-AXVolume: 3mLBed Weight: 60mgSample Dilute 1mg ethametsulfuron in Pretreatment: 100mL 2% ammonia aqueous solution (10 ppm)Conditioning: 1mL methanol followed by 1mL deionized waterApplication: Load 2 mL sampleWashing: 1mL of 2% ammonia hydroxide solution followed by 1mL methanolElution: 2mL methanol with 2% acetic acid

Pharmaceutical and Biochemical

Cyclosporin from BloodCompounds: CyclosporinPart Number: 60108-304Phase: HyperSep C18Volume: 3mLBed Weight: 500mgSample Mix 1mL heparinised blood Pretreatment: with 2mL water/acetonitrile

(7:3, v/v). Stir mixture and centrifuge after 5 min.

Conditioning: 3mL acetonitrile followed by 3mL water/acetonitrile (8:2, v/v)Application: Force or aspirate the sample slowly

through columnWashing: 0.5 M acetic acid/acetonitrile (8:2, v/v) followed by 0.5 M acetic acid/acetonitrile (6:4, v/v)Elution: Acetonitrile

Gabapentin in Serum, Plasma or Whole BloodCompounds: GabapentinPart Number: 60108-302Phase: HyperSep C18Volume: 1mLBed Weight: 100mgSample 500µL sample, calibrator or Pretreatment: control to be placed into a glass test

tube. Add 25µL internal standard (5.0mg/L). Add 500µL 20% acetic acid and vortex tube.

Conditioning: 3mL CH3OH followed by 3mL deionised water and 1mL 100mM HCl

Application: Load sample at 1-2mL/min.Washing: 3mL deionized water followed by 3mL ethyl acetate and 3mL hexane.

Dry column under vacuum for 30 secElution: 1mL 2% NH4OH in CH3OH Evaporate to dryness at <40 °C

Ketamine in UrineCompounds: KetaminePart Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 2mL urine add internal standard Pretreatment: and 1mL 100mM phosphate

buffer (pH 6). Mix/vortex. Use 100mM monobasic or dibasic sodium phosphate to ensure sample pH of 6

Conditioning: 3mL CH3OH followed by 3mL deionized water and 1mL 100mM phosphate buffer (pH 6)Application: Load at 1mL/min.Washing: 3mL deionized water 1mL 100mM acetic acid 3mL CH3OH Dry column (5 min at > 10 inches Hg)Elution: 3mL dichloromethane/isopropanol/

ammonium hydroxide (78:20:2) – collect eluents at 1-2mL/min. using minimal vacuum Evaporate to dryness at <40°C

Methylmalonic Acid (MMA) from Serum or Plasma for GCMS AnalysisCompounds: Methylmalonic acid (MMA)Part Number: 60107-403Phase: HyperSep Retain-AXVolume: 3mLBed Weight: 60mgSample To 250μL serum or plasma add 100μL Pretreatment: D3MMA (2.5μM/L) and 1.5mL waterConditioning 2mL methanol followed by 2mL waterApplication: Load samples at 1-2mL/min.Washing: 2mL water followed by 2mL methanol with 0.5% acetic acidElution: 2mL Tert Butyl Methyl Ether (TBME) with 3% formic acid. Evaporate to dryness at <40ºC.Derivatization: Add 50μL acetonitrile and 25μL MTBSTFA + 1% TBDMCl. Heat at 70ºC for 10 mins.Source: Department of Laboratory Medicine, The Hospital of Telemark

130

536_Page 130-131.indd 130 12/10/2009 6:35:06 PM

creo

Antineoplastic Agents from PlasmaCompounds: Bisantrene, mitoxantronePart Number: 60108-302Phase: HyperSep C18Volume: 1mLBed Weight: 100mgConditioning: 2mL methanol followed by 2mL distilled waterApplication: Force or aspirate 1-2mL plasma slowly through columnWashing: 2mL distilled waterElution: 2 x 200µL volumes 0.5M methanolic HCl

Antiarrhythmic Drug Flecainide from PlasmaCompounds: FlecainidePart Number: 60108-392Phase: HyperSep C8Volume: 1mLBed Weight: 100mgSample Mix 1mL plasma with 1mL water Pretreatment: and 200µL 0.2M sodium carbonate

solutionConditioning: 2mL methanol followed by 2mL distilled waterApplication: Force or aspirate sample slowly through columnWashing: 2mL distilled waterElution: 500µL methanol, then elute from column after 1 min.

Antiepileptics from SerumCompounds: Carbamazepine, dilantin,

phenobarbital, primidonePart Number: 60108-302Phase: HyperSep C18Volume: 1mLBed Weight: 100mgSample Mix 500µL serum with 500µL Pretreatment: 4-methylprimidone in citrate buffer

pH 4 (internal standard)Conditioning: 2mL methanol followed by

2mL waterApplication: Force or aspirate sample slowly through columnWashing: 2 column volumes distilled waterElution: 2 x 100µL volumes acetone

Catecholamine Metabolites from UrineCompounds: Vanillylmandelic acid,

homovanillic acidPart Number: 60108-521Phase: HyperSep SAXVolume: 3mLBed Weight: 500mgSample Collect 24h urine (preserved Pretreatment: with 0.1M HCl). Store at 4°C Dilute

sample prior to extraction 1:1 with water. Use 0.5M NaOH to adjust pH to 7.5.

Conditioning: 6mL methanol followed by 6mL distilled waterApplication: Force or aspirate pretreated

sample through columnWashing: 6mL distilled waterElution: 6mL 1.5M sodium hydroxide

solution


Sample Preparation

536_Page 130-131.indd 131 12/10/2009 6:35:08 PM

creo

Environmental

Cyanuric Acid in Drinking WaterCompounds: Cyanuric AcidPart Number: 60106-402Phase: HyperSep HypercarbVolume: 6mLBed Weight: 500mgSample Adjust water sample to pH 3 Pretreatment: Conditioning: Wash column with 10mL methanol Condition column with 10mL LC-grade waterApplication: Force/aspirate 250-500mL of

water sample into column at rate of 5mL/min.

Washing: Dry column under vacuumElution: 20mL methanol – evaporate to dryness at 50°C under nitrogenSource: Marie Claire Hennion, ESPCI, Paris

Organochlorine Insecticides from WaterCompounds: Aldrin, p,p’-DDE, o,p’-DDE, o,p’-DDT,

p,p’-DDT, dieldrin, endosulfan I, endosulfan II, endrin, heptachlor, heptachlor epoxide, lindane, p,p’-methoxychlor

Part Number: 60108-305Phase: HyperSep C18Volume: 6mLBed Weight: 500mgSample Filter sample if required Pretreatment: Conditioning: 12mL ethyl acetate followed by

6mL methanol and 6mL distilled waterApplication: Force or aspirate sample slowly

through column.Washing: 6mL distilled water – dry column under vacuum for 15 min.Elution: 2 x 500µL ethyl acetate

Concentrate eluate to 250µL in stream of nitrogen at 40°C.

Explosives from WaterCompounds: 1,3-dinitrobenzene, 2,6-dinitrotoluene,

2,4-dinitrotoluene, nitrobenzene, RDX (hexahydro-1,3,5-trinitro-s-triazine), tetryl (N-methyl-N,2,4,6-tetranitroaniline), 1,3,5-trinitrobenzene, 2,4, 6-trinitrotoluene

Part Number: 60108-305Phase: HyperSep C18Volume: 6mLBed Weight: 500mgSample Adjust 500mL water sample Pretreatment: to pH 6 Dissolve 150g NaCl in the

sample and filterConditioning: 12mL methanol followed by 12mL waterApplication: Force or aspirate sample slowly through columnWashing: 1mL distilled water. Dry column under vacuum for 5 min.Elution: 2 x 1mL volumes methanol

Extraction of Tear GasCompounds: Chloroacetophenone (cs),

o-chlorobenzylidenemalonitrile (cn), trans-8-methyl-n-vanillyl-6-nonen-amide (oc)

Part Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample Clothing: Cut out portion of sprayed Pretreatment: area and a ‘negative’ control sample.

Extract each into hexane Canisters: Spray onto a Kimwipe™ and extract sprayed area and a negative control sample into hexane.

Conditioning: 3mL CH3OH followed by 3mL deionized water and 1mL 100mM phosphate buffer (pH 6)

Application: Load at 1mL/min.Washing: 3mL deionized water and 3mL hexane.

Dry column for 5 min at >10 inches HgElution: 1mL CH3OH Evaporate to dryness at <40°C

132

Chlorophenoxy Acid Herbicides in Water for GC or GCMS AnalysisCompounds: 2,4-D Acid, 2,4,5-trichloro phenoxy propionic acid (Silvex), Dicamba, Dinitro-sec-butyl phenolPart Number: 60108-301Phase: HyperSep C18Volume: 6mLBed Weight: 1gSample Adjust pH of 1 litre of water sample Pretreatment: to pH 1.0 with hydrochloric acid.Conditioning 10mL Hexane / Acetone (50:50) 10mL Acidified Methanol (5% HCl in MEOH) 10mL distilled WaterApplication: Load 1 Litre of pH adjusted water sample at a rate of 8-10mL/min.Washing: 10mL Distilled Water adjusted to pH 1.0 with HCl. Dry column under maximum vacuum pressure for 15-30 minutes.Elution: 10mL of Hexane / Acetone (50:50)Concentration/ Add 500μL of a keeper solvent (meth- Evaporation: anol, DMF, other). Evaporate to 500μL under a nitrogen stream at room tem perature. Reconstitute with 100μL TCTEF. Inject at 1-2μL onto GC

Polynuclear Aromatic Hydrocarbons in Pond Water for GC or GCMS AnalysisCompounds: Napthene, Fluorene, Acenapthene, Phenanthrene, Anthracene, Fluoranthene, Pyrene, B(a)anthracene, Chrysene, B(e)pyrene, B(b)fluoranthene, B(k)fluoranthene, B(a)pyrene, D(a,h)anthracene, B(g,hi)perylene, Indeno(1,2,3,-cd)pyrenePart Number: 60108-518Phase: HyperSep AminopropylVolume: 3mLBed Weight: 500mgSample Filter water through a 0.5μm filter. Pretreatment: Add 2mL methanol to 200mL of filtered water sample. Mix and de-gas the sample for 2 minutes.Conditioning 15-20mL Methylene Chloride / Trichlorotrifluoroethylene (TCTFE) 15-20mL TCTFE then dry for 5 minutes 15-20mL Methanol 20mL Distilled WaterApplication: Load 200mL water sample at a rate of 8-10mL/min.Washing: 20mL Distilled Water. Dry column for 15-30 minutes under maximum vacuum pressureElution: 20mL TCTFEConcentration/ Evaporate to dryness under a nitrogen Evaporation: stream at room temperature. Reconstitute with 100μL TCTEF. Inject at 1-2μL onto GC

536_Page 132-133.indd 132 12/10/2009 6:36:20 PM

creo

Trace Metal Elements from WaterCompounds: Bi, Cd, Co, Cu, Fe, Hg, Mn, Mo, Ni, Pb, TiPart Number: 60108-388Phase: HyperSep PhenylVolume: 3mLBed Weight: 500mgSample Adjust 500mL water to pH 8-9 Add Pretreatment: 1mL 0.1% aqueous sodium diethyl dithiocarbamate solutionConditioning: 3mL methanol followed by

3mL waterApplication: Force or aspirate sample through column at rate of 3-4mL/min.Washing: 2mL distilled water. Dry column under vacuum for 3-4min.Elution: 6mL Methanol

Pesticides and PAHs from WaterCompounds: Pesticides, PAHsPart Number: 60108-302Phase: HyperSep C18Volume: 1mLBed Weight: 100mgConditioning: 1mL methanol followed by 1mL distilled waterApplication: Force or aspirate 50-100mL water through columnWashing: Dry column under vacuumElution: Pour 500µL ethyl acetate into

column. Allow to percolate without vacuum. Collect eluate for subsequent analysis

Forensics/Toxicology

Carboxy D9 Tetrahydrocannibinol (THC) in UrineCompounds: Carboxy D9 THCPart Number: 60108-304Phase: HyperSep C18Volume: 3 mLBed Weight: 500 mgSample To 5 mL urine add 0.5 mL 10N KOH Pretreatment: Heat at 55 °C for 15 min and then

cool. Add 1 mL glacial acetic acid.Conditioning: 6 mL methanol followed by 6 mL 0.01N HClApplication: Force or aspirate sample slowly through columnWashing: 2 x 500 µL volumes acetonitrile/

0.01N HCl (60:40, v/v). Evaporate to dryness under vacuum

Elution: 2 x 500 µL volumes n-heptane/ethyl acetate (85:15, v/v)

Amphetamines, Opiates and Phencyclidine in Oral FluidCompounds: Amphetamines, opiates,

phencyclidinePart Number: 60108-741Phase: HyperSep Verify-CXVolume: 1mLBed Weight: 50mgSample Add 100-500 µL neat sample to a Pretreatment: clean tube. Add internal standard

and leave for 10 min at ambient temperature. Add 800µL 100 mM phosphate buffer pH 6 Mix/vortex for 10 sec. Use 100 mM monobasic or dibasic sodium phosphate to ensure pH 6.

Conditioning: 200µL CH3OH 200µL deionized water 200µL 100 mM phosphate buffer pH 6

Application: Do not exceed 1 mL/minWashing: 500µL deionized water

500µL 100 mM acetic acid 500µL CH3OH. Dry column

Elution: 800µL CH2Cl2/IPA/NH4OH (70:26:4) Do not exceed 1mL/min Prepare elution solvent daily

Therapeutic and Abused Drugs in Urine for Acid/Neutral and Basic DrugsCompounds: Barbiturates, ibuprofen, cotinine, amphetamine, codeine, ketaminePart Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 2mL urine add internal Pretreatment: standard(s) and 1 mL 100 mM phos-

phate buffer (pH 6). Mix/vortex. Use 100 mM monobasic or dibasic sodium phosphate to ensure sample pH of 6.

Conditioning: 3mL CH3OH 3mL deionized water 2mL 100 mM phosphate buffer (pH 6) Aspirate at < 3 inches Hg

Application: Load at 1 to 2mL/minWashing: 3mL deionized water

1mL 100 mM acetic acid Dry column (5 min at >10 inches Hg) 2mL hexane

Elution: Acidic and Neutral: 3mL hexane/ethyl acetate (50:50) Collect eluate at < 2mL/min Evaporate to dryness at < 40°C Basic: 3mL CH2Cl2/IPA/NH4OH (78:20:2). Collect eluate at 1-2mL/min Evaporate to dryness at < 40°C using evaporator


Sample Preparation

536_Page 132-133.indd 133 12/10/2009 6:36:21 PM

creo

Drugs from Blood and UrineCompounds: Amphetamines, barbiturates,

opiatesPart Number: 60108-304Phase: HyperSep C18Volume: 3mLBed Weight: 500mgSample Adjust 10mL urine to desired Pretreatment: pH value using HCI or ammonia

and centrifuge Amphetamines and Opiates: pH 8-9 Active components: pH 7-8 Barbiturates: pH 7

Conditioning: 6mL methanol followed by 6mL Distilled water pH7Application: Force or aspirate sample slowly through columnWashing: 6mL water. Dry column under

vacuum for 5 min.Elution: Aspirate 750µL eluent into column.

Elute after 1 min. and then flush with 750µL eluent. Eluents: Cannabinoids: acetone. Barbiturates, active components, bases, amphetamines: acetone/chloroform (1:1)

Methadone in UrineCompounds: Methadone Part Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 2mL urine add internal Pretreatment: standard(s) and 1mL 100mM

phosphate buffer (pH 6). Mix/vortex. Use 100mM monobasic or dibasic sodium phosphate to ensure sample pH of 6.

Conditioning: 3mL CH3OH 3mL deionized water 2mL 100mM phosphate buffer (pH 6) Aspirate at < 3 “Hg

Application: Load at 1 to 2mL/min.Washing: 3mL deionized water

1mL 100mM acetic acid 3mL CH3OH Dry column (5 min at >10 “Hg)

Elution: 3mL CH2Cl2/IPA/NH4OH (78:20:2) Collect eluate at 1-2mL/min. Prepare elution solvent daily

Forensics/Toxicology

Beta Agonists in Urine – for GC or GC/MS AnalysisCompounds: Beta AgonistsPart Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 1mL urine add 2mL of 0.1M Pretreatment: Acetate buffer at pH 4.7.Conditioning 3mL methanol then aspirate 3mL DI water then aspirate 1mL 0.1M acetate buffer (pH 4.7). NOTE: Aspirate at < 3 “Hg to prevent sorbent dryingApplication: Load sample at 1-2mL/min.Washing: 2 x 1mL Acetone / Methanol (1:1) then aspirate. Dry column (5 minutes at > 10 “Hg)Elution: 1mL CH2Cl2 / IPA / NH4OH (82/16/2). Collect the eluate by gravity feed. NOTE: Prepareelution solvent fresh daily. Add IPA / NH4OH mix, then add CH2Cl2 (pH 11-12). Evaporate to dryness at <40ºC.Derivatization: Methaneboronic acid at 5mg/mL prepared in dry ethyl acetate (use molecular sieve) stored at -20ºC (freezer conditions) until use. Add 100μL of the methaneboronic acid solution then mix / vortex. React for 15 minutes at 70ºC. Remove from heat source to cool. NOTE: Do not evaporate this solution.

Benzodiazepines from SerumCompounds: BenzodiazepinesPart Number: 60108-302Phase: HyperSep C18Volume: 1mLBed Weight: 100mgSample Mix 500µL serum with 100µL Pretreatment: 0.1M sodium carbonate solution.

Add internal standard if requiredConditioning: 2mL methanol followed by 2mL distilled waterApplication: Force or aspirate sample slowly through columnWashing: 2mL distilled water followed by 50µL methanolElution: 2 x 200 µL volumes methanol

Amphetamines in UrineCompounds: AmphetaminesPart Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 2mL urine add internal Pretreatment: standard(s), 1mL 100mM

phosphate buffer pH 6 and 1mL 0.35M sodium periodate. Mix/vortex and incubate at ambient temperature for 20 min. Use 100mM monobasic or dibasic sodium phosphate to ensure sample pH of 6.0±0.5.

Conditioning: 3mL CH3OH followed by 3mL deionised water and 1mL 100mM phosphate buffer (pH 6) Aspirate at < 3 “Hg to prevent sorbent drying

Application: Load at 1 to 2mL/min.Washing: 3mL deionized water followed by

1mL 100 mM acetic acid and 3mL CH3OH. Dry column (5 min at >10 “Hg)

Elution: 3mL CH2Cl2/IPA/NH4OH (78:20:2) Collect eluate at 1 to 2mL/min. Prepare elution solvent daily Concentrate eluate by adding 30µL silation grade DMF to eluate. Evaporate to 30µL at <40°C

134

536_Page 134-135.indd 134 12/10/2009 6:37:32 PM

creo

Abused Drugs in Equine UrineCompounds: Aminocaproic acid

(6-aminohexanoic acid)Part Number: 60108-421Phase: HyperSep SCXVolume: 1mLBed Weight: 100mgSample Mix 1mL urine with 1mL phosphoric Pretreatment: acid (7mM). Use concentrated H3PO4

to adjust pH to 2Conditioning: 1mL methanol followed by 1mL

distilled water and 1mL 7mM H3PO4Application: Force or aspirate sample slowly

through column Dry column under vacuum for 30 sec.

Washing: 1mL 7mM phosphoric acid 0.5mL 0.1M acetic acid 1mL methanol Dry column under vacuum for 30 sec.Elution: 2 x 1 mL ammoniacal

methanol (1%)

Opiates in Urine – Oxime TMS Procedure for GC or GC/MS AnalysisCompounds: Codeine TMS, Morphine TMS, Hydrocodone Oxime TMS, Oxycodone- Oxime TMS, Oxmorphone-Oxime TMSPart Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 2mL urine add internal standard(s)* Pretreatment: and 400μL concentrated HCl. Add 200μL 10% Hydroxylamine Solution. (Acid Mix / Vortex. Hydrolysis of Heat to 90ºC for 20 min. in a heating Glucuronides) block or an autoclave for 15 minutes on a liquid cycle. Cool before proceed ing. Centrifuge for 10 minutes 1t 2,000rpm and discard pellet. Add 500μL 50% Ammonium Hydroxide, then mix/vortex. Adjust sample pH to 6.0-7.0 by drop-wise addition with 50% Ammonium HydroxideConditioning: 3mL methanol then aspirate 3mL DI water then aspirate 2mL 0.1M phos phate buffer (pH 6.0) then aspirate NOTE: Aspirate at < 3 “Hg to prevent sorbent dryingApplication: Load sample at 1-2mL/min.Washing: 3mL DI water then aspirate 3mL 0.1M acetate buffer (pH 4.5) then aspirate 3ml methanol then aspirate Dry column (5 minutes at >10 “Hg)Elution: 3mL CH2Cl2/IPA/NH4OH (78/20/2); collect eluate at 1-2ml/min. NOTE: Prepare elution solvent daily. Add IPA/NH4OH, them mix, then add CH2Cl2 (pH 11-12). Evaporate to dryness at <40ºC.Derivatization: Add 100μL ethyl acetate and 50μL MSTFA (Part number TS-38831) Overlayer with N2 and cap. Mix/vortex. React 20 minutes at 85ºC in a heat block. Remove from heat source to cool. NOTE: Do not evaporate MSTFA solution


Sample Preparation

Nicotine and Cotinine in Urine or Serum – for GC or GC/MS AnalysisCompounds: Beta AgonistsPart Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 2mL urine or serum add internal Pretreatment: standard(s) and 2mL of 0.1M phos phate buffer (pH 6.0). Mix / vortex.Conditioning 3mL methanol then aspirate 3mL DI water then aspirate 2mL 0.1M phosphate buffer (pH 6.0) then aspirate. NOTE: Aspirate at < 3 “Hg to prevent sorbent dryingApplication: Load sample at 1mL/min.Washing: 3mL DI Water then aspirate 1mL 0.1M acetic acid then aspirate Dry column (5 minutes at >10 “Hg). 2mL Hexane then aspirateElution 3mL Hexane / Ethyl Acetate (50:50), (Cotinine): collect at 1-2mL/min.Washing: Remove rack of collection tubes to re-wash columns 3mL Methanol then aspirate Dry column, 5 minutes at >10 “Hg.Elution Replace rack of collection tubes (Nicotine): 3mL CH2Cl2 / IPA / NH4OH (78/20/2); collect eluate at 1mL/minute. NOTE: Prepare elution solvent fresh daily. Add IPA / NH4OH mix, then add CH2Cl2 (pH 11-12). Evaporate to dryness at <40ºC. Take care not to over-heat or over-evaporate.Derivatization: 100μL Methanol Inject 1-2μL onto the GC.

536_Page 134-135.indd 135 12/10/2009 6:37:33 PM

creo

Flunitrazepam & Metabolites in Urine – for GC or GC/MS AnalysisCompounds: Flunitrazepam, 7-aminoflunitrazepam, desmethylflunitrazepamPart Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 2mL urine add internal standard(s)* Pretreatment: and 1mL ß-glucuronidase solution. ß-glucuronidase solution contains 5,000 F units/mL Patella Vulgata in 0.1M acetate buffer (pH = 5.0). Mix / vortex. Hydrolyze for 3 hours at 65ºC. Centrifuge for 10 minutes at 2,000 rpm and discard pellet. Cool before proceeding.Conditioning: 3mL methanol then aspirate 3mL DI water then aspirate 1mL 0.1M phosphate buffer (pH 6.0). NOTE: Aspirate at < 3 “Hg to prevent sorbent dryingApplication: Load sample at 1-2mL/min.Washing: 2mL DI water then aspirate 2mL 20% acetonitrile in 0.1M phos phate buffer (pH 6.0) then aspirate Dry column (5 minutes at > 10 “Hg). 2mL hexane then aspirateElution: 3mL ethyl acetate with 2% NH4OH; collect eluate at 1 to 2mL/minute. Prepare fresh daily. Evaporate to dry ness at <40ºC.Derivatization: Add 50μL ethyl acetate and 50μL MTBSTFA (with 1% TBDMCS) (Part number TS-48927) Overlayer with N2 and cap. Mix/vortex. React 20 minutes at 70ºC. Remove from heat source to cool. NOTE: Do not evaporate MTBSTFA solution.

Phencyclidine in Urine – for GC or GC/MS AnalysisCompounds: PhencyclidinePart Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 2mL urine add internal standard(s) Pretreatment: and 1mL of 0.1M phosphate buffer (pH 6.0). Mix / vortex. Sample pH should be 6.0±0.5. Adjust pH accord ingly with 0.1M monobasic or dibasic sodium phosphate.Conditioning 3mL methanol then aspirate 3mL DI water then aspirate 1mL 0.1M phosphate buffer (pH 6.0) then aspirate NOTE: Aspirate at <3 “Hg to prevent sorbent drying.Application: Load sample at 1-2mL/min.Washing: 3mL DI Water then aspirate. 1mL 0.1M acetic acid then aspirate 3mL Methanol then aspirate Dry column (5 minutes at >10 “Hg).Elution: 1mL CH2Cl2 / IPA / NH4OH (78/20/2). Collect the eluate by gravity feed. NOTE: Prepare elution solvent fresh daily. Add IPA / NH4OH mix, then add CH2Cl2 (pH 11-12). Evaporate to dryness at <40ºC. Remove immediately upon completion.Derivatization: 100μL ethyl acetate. Inject 1-2μL onto the GC.

4’4-Methylenedianaline in Serum – for LC or LCMS AnalysisCompounds: 4’4-MethylenedianalinePart Number: 60108-302Phase: HyperSep C18Volume: 1mLBed Weight: 100mgSample None Pretreatment:Conditioning 3mL methanol 3mL DI Water Application: Load sample at 1mL/min.Washing: 1mL DI WaterElution: 0.25mL Methanol containing 1M ammonium hydroxideInjection / Inject 10μL onto the HPLC system analysis: 4’4-Methylenedianaline – extracted 100μg/mL sampleRetention time = 3.162 minutesMobile phase: Methanol / water (50:50) C18 column Flow rate = 1.2mL/min. Inj. Volume = 10μL Wavelength = 254nm

Methadone in Urine – for GC or GC/MS AnalysisCompounds: MethadonePart Number: 60108-742Phase: HyperSep Verify-CXVolume: 10mLBed Weight: 200mgSample To 2mL urine add internal standard(s) Pretreatment: and 1mL of 0.1M phosphate buffer (pH 6.0). Mix / vortex. Sample pH should be 6.0±0.5. Adjust pH accordingly with 0.1M monobasic or dibasic sodium phosphate.Conditioning: 3mL methanol then aspirate 3mL DI water then aspirate 1mL 0.1M phosphate buffer (pH 6.0) then aspirate NOTE: Aspirate at <3 “Hg to prevent sorbent drying.Application: Load sample at 1-2mL/min.Washing: 3mL DI Water then aspirate. 1mL 0.1M acetic acid then aspirate 3mL Methanol then aspirate Dry column (5 minutes at > 10 “Hg).Elution: 1mL CH2Cl2 / IPA / NH4OH (78/20/2). Collect eluants at 1-2mL/min. using minimal vacuum NOTE: Prepare elution solvent fresh daily. Add IPA / NH4OH mix, then add CH2Cl2 (pH 11-12).Derivatization: 100μL ethyl methanol Inject 1-2μL onto the GC.

136

536_Page 136-137.indd 136 12/10/2009 6:38:27 PM

creo

Food Safety

Water Soluble Vitamins from Aqueous SolutionsCompounds: Niacinamide, pyridoxine, riboflavin, thiaminePart Number: 60108-304Phase: HyperSep C18Volume: 3mLBed Weight: 500mgSample Use an amber glass bottle. Pretreatment: Mix 50mL sample with riboflavin con-

tent < 6mg with 0.5mL acetic acid and 0.1g heptane-1-sulphonic acid sodium salt. Flush bottle with nitrogen, heat to 55°C, shake. Cool down rapidly.

Conditioning: 3mL Methanol followed by 3mL of a solution of 0.5mL acetic acid and 0.1g heptane-1-sulfonic acid sodium salt in 50mL water (to be prepared daily)

Application: 2mL sample solution to be forced or aspirated through column

Washing: 2 x 250µL of a solution of 0.5mL ace-tic acid and 0.1g heptane-1-sulphonic acid sodium salt in 50mL water

Elution: 3 x 500µL methanol – Immediate analysis required

Aflatoxin M1 Mycotoxin from MilkCompounds: Aflatoxin M1Part Number: 60108-305Phase: HyperSep C18Volume: 6mLBed Weight: 500mgSample Dilute 20 mL milk with 30mL Pretreatment: distilled waterConditioning: 10mL methanol followed by

10mL distilled waterApplication: Force or aspirate sample slowly through columnWashing: 10mL distilled water followed by

10mL n-hexane – then dry column at 50°C for 10-20 min or at ambient temp overnight

Elution: 3mL dichloromethane/ acetone (4:1, v/v)

Anthocyan Dyes from Red WineCompounds: Anthrocyan dyePart Number: 60108-309Phase: HyperSep C8Volume: 3mLBed Weight: 500mgConditioning: 3mL methanol followed by 3mL distilled waterApplication: Force or aspirate wine sample slowly through columnWashing: 1.5mL distilled waterElution: Small volume methanolic HCl

Folic Acid from FoodCompounds: Folic acidPart Number: 60108-521Phase: HyperSep SAXVolume: 3mLBed Weight: 500mgSample Homogenize 10 g food sample Pretreatment: in 100 mL 0.01 M phosphate buffer

pH 7.4. FilterConditioning: 6mL n-hexane followed by 6mL methanol and 6mL distilled waterApplication: Force or aspirate 10mL filtrate through columnWashing: 6mL distilled waterElution: 5mL 10% NaCl in 0.1M sodium acetate buffer


Sample Preparation

536_Page 136-137.indd 137 12/10/2009 6:38:36 PM

creo

Problem Causes Recommended Solutions

Poor Recovery of Analytes Improper conditioning. Condition column according to phase: Reversed phase: use methanol, acetonitrile or isopropanol followed by

solvent used for sample solution. Ion exchange: use methanol or isopropanol followed by a buffer at a pH which charges both sorbent and analyte.

Analytes have a greater affinity for the sample solution Choose a column having a greater selectivity for analytes. than for the column. Change pH of sample to increase affinity of analytes to sorbent. Change polarity of sample solvent to produce lower affinity for the analytes. Improper washing. Wash column using appropriate wash solution. Poor elution. Increase eluent volume, increase eluent strength. Decrease elution strength of washing solvent.

Analytes Not Eluted from Column Analytes have stronger interaction with column Choose a column that is less retentive for the analytes. sorbent than with eluting solvent. Elution volume is too low. Increase the elution volume. Elution solvent is too weak to disrupt analyte Change pH of eluting solvent to ensure greater affinity for interaction with the column. the analytes. Change polarity of eluting solvent to ensure greater affinity for the analytes.

Inconsistent Extraction Column dries before sample added. Re-condition column. Capacity of column is exceeded. Decrease sample volume. Use a column with a larger amount of sorbent. Sample loading flow rate is too high. Decrease flow rate. Elution flow rate is too fast. Allow elution solvent to seep into column before aspirating or forcing

through column. Apply in two separate aliquots versus a single aliquot. Using too strong a wash solution to remove interferences. Reduce strength of wash solvent. Elution volume is too small. Increase volume of elution solvent.

Interferences Eluted with Analytes Interferences are extracted at the same time as analytes. Selectively wash interferences from column prior to elution. Use a column which retains the analytes more than the interferences. Interference is due to leachables from column. Wash column with eluting solvent prior to conditioning.

Slow Column Flowrate Excessive particulate matter in sample. Filter or centrifuge sample. Sample solution is too viscous. Dilute sample with a weak solvent. Inadequate vacuum. Increase vacuum.

Large Volume of Sample Required Sample volumes larger than extraction column capacity. Use larger extraction column volumes.

Low Throughput Improper methodology. Consider a different extraction mechanism sorbent or format.

Determine appropriate column volume

The column volume referred to in the ordering information is the maximum volume of sample or solvent that can be applied to the SPE tube or well per aliquot. The most appropriate column volume for a

SPE Troubleshooting

Other Considerations for SPE Method Developmentspecific method will allow for easy application of the sample and will closely match the necessary volume of wash solvent for the method.

Determine the appropriate bed weight

The bed weight defines the maximum amount of analyte a sorbent can retain.

Bed weights should be chosen based on the sample size, taking into consideration the approximate amount of contaminants present and the separation mode. For normal and reversed phase modes, typical bed capacity is 1 - 5% of the bed weight, up to 100mg/g packing for strongly retained compounds. For example, a 500mg bed weight can retain up to 25mg of solute mass. For less retained compounds, if the bed weight is too small relative to the sample matrix volume, breakthrough (early elution) can occur.

For ion exchange sorbents, the number of charged sites available for interaction with the analyte determines the capacity. This ionic capacity of an ion exchange sorbent is given in mEq/g. The mean ionic capacity values range from 0.072 to 0.320mEq/g.

138

536_Page 138-139.indd 138 12/10/2009 6:39:35 PM

creo

SPE Technical SectionThe Role of pH in SPE:Reversed Phase Applications:

• If trapping of the analyte in the tube is desired, the pH of the conditioning solvent should be adjusted

• If the compound of interest is acidic or basic – use pH at which compound is not charged

• The retention of neutral compounds is not usually affected by pH

Normal Phase Applications:

• pH is not normally an issue in normal phase applications

• Solvents used are typically non-polar organic solvents, rather than water

• There is no need to verify sample application pH

Ion Exchange Applications:

• pH and pKa are important considerations here

• Acidic compounds are extracted by an anion-exchange columne from a sample solution 1-2 pH units above the pKa of the analyte

• Basic compounds are extracted by a cation-exchange column from a sample solution 1-2 pH units below the pka of the analyte

• Adjust pH accordingly using appropriate buffers

Recommended Solvents:

• Non-polar solvents such as THF are strong solvents in reversed phase interactions

• Polar solvents such as methanol and water are strong solvents in normal phase interactions

• The choice of solvent is dependent upon application and analyte properties

Key Terms used in SPE:

Term Definition

Analyte Compound to be isolated & measured in the SPE systemBed Volume The sum of the interstitial volume plus the pore volume of the sorbent within the SPE columnBreakthrough Lack of analyte retention which occurs when the total mass of the solutes (analytes & interferences) exceeds the capacity of the sorbentCapacity Total quantity of compounds (analytes & interferences) which can be retained from a specific sample matrix solution by a given mass of sorbentEluent Solvent used for elution in SPE to remove the analytes from the solid phaseInterference Substance in the sample of separation system which may influence the retention of the analyte on the sorbent or may co-elute with the analyte and influence analytical determinationMatrix All components of the sample including the solvent, but excluding the analytesSorbent Bonded phase silica or adsorbent used as the stationary phase in SPESurface Area Sum of the external surface area of a sorbent particle plus the accessible surface area within the pores (m2/g)

Solvent Polarity

Hexane Non-polarIsooctanePetroleum etherCyclohexaneCarbon tetrachlorideChloroformMethylene chlorideTetrahydrofuranDiethyl etherEthyl acetateAcetoneAcetonitrileIsopropanolMethanolWaterAcetic acid Polar


Sample Preparation

536_Page 138-139.indd 139 12/10/2009 6:39:58 PM

creo

140

}National Scientific Target Syringe Filters

Syringe Filter Membrane Selection GuideChoose a filter or membrane based on:

1. Chemical compatibility of the membrane and housing with your sample matrix2. Size and amount of particulates in the sample3. Potential interactions (binding) between the membrane and sample components4. Special considerations such as requirement for pre-filter or inorganic ion certification

Target Syringe Filter Housings

• Target™ Syringe filter housings are manufactured from solvent-resistant, low-extractable polypropylene resins specifically selected for wide compatibility with common HPLC sample matrices.• Solutions at temperatures up to 100°C can be filtered using Target syringe filters.• Target syringe filters can be sterilized by autoclave at 125°C for 15 minutes.• The inlet connection is an enhanced female Luer-Lok™ fitting designed for extra security when attached to a Luer-Lok syringe.• The outlet fitting is a standard size male Luer-slip fitting for ease of filtrate collection.• Target polypropylene syringe filter housings meet the requirements of 21 CFR 177.1520.

This table offers general guidelines for membrane characteristics and compatible applications.

Membrane Type Membrane Characteristics Applications

Cellulose Acetate Low protein binding, ideal for aqueous-based samples; high protein recovery from Tissue Culture media filtration, sensitive filtrate; lower protein binding compared to PVDF biological samplesGlass MicroFiber Larger porosity; able to remove large particulates without clogging Dissolution testing, general filtrationNylon Most frequently selected membrane; broad compatibility with aqueous and organic General laboratory filtration; filtration for most samples; naturally hydrophilic membrane; extremely low in extractables; excellent HPLC samples. NOTE: Nylon binds protein, flowrate with most sample matrices; not compatible with strong acids or bases do not use when high protein recovery is desiredPolyethersulfone High flowrates with good throughput volume; low protein binding; compatible with high PES is certified for Ion Chromatography; temperature liquids; mechanically strong membrane low in inorganic extractable ions Tissue Culture filtration; filtration of proteins and nucleic acidsPolypropylene Hydrophilic membrane has wide chemical compatibility with organic solvents; Filtration of biological samples; filtration of low nonspecific protein binding aggressive organic solutionsPTFE Hydrophobic membrane is resistant to nearly all solvents, acids, and bases; membrane is Filtration of aggressive organic, highly basic or mechanically strong and will withstand exposure to high temperature liquids; hot solutions, ideal for transducer protectors low in extractables; PTFE blocks water vapor; can be used to filter aqueous solutions after prewetting with an alcohol PVDF Hydrophilic membrane with good solvent resistance; low UV absorbing extractables General biological filtration; filtration of and low nonspecific binding samples where high protein recovery is desiredRegenerated Cellulose Hydrophilic membrane with good solvent resistance, extremely low nonspecific binding; Membrane of choice for low nonspecific compatible with nearly all common HPLC solvents; tolerates aqueous samples in binding applications; Tissue Culture media pH range of 3 to 12 filtration and general biological sample filtration

Go to: paGe 8See our range of Certified Vials

536_Page 140-141.indd 140 12/10/2009 6:41:47 PM

creo


Sample Preparation

Nylon Syringe FiltersNaturally hydrophilic membrane offers broad compatibiity with aqueous and organic samples

Solvent-resistant, low-extractable polypropylene housing. Syringe filters can be sterilized by autoclave at 125° for 15 minutes.

}Membrane: HPLC Certified Nylon

}Prefilter: binder-free glass microfiber

}Connections: enhanced female Luer-Lok inlet, male slip outlet

}Max. operating temperature: 100°C

}Max. operating pressure: 4mm - 75psi, 17mm - 115psi, 30mm - 90psi

}Retention volumes: 4mm - <15µL, 17mm - <29µL, 30mm - <137µL

Applications:

• General laboratory filtration

• Filtration for most HPLC samples

National Scientific Target Nylon Syringe FiltersOutside Dia. Pore Size Prefilter Cat. No. Quantity4mm 0.45µm No F2504-1 100 Pack4mm 0.2µm No F2504-2 100 Pack17mm 0.45µm No F2513-1 100 Pack17mm 0.2µm No F2513-2 100 Pack30mm 0.45µm No F2500-1 100 Pack30mm 0.2µm No F2500-2 100 Pack30mm 1.5µm No F2500-12 100 Pack30mm 5.0µm No F2500-50 100 Pack30mm 0.45µm Yes F2502-1 100 Pack30mm 0.2µm Yes F2502-2 100 Pack

536_Page 140-141.indd 141 12/10/2009 6:41:51 PM

creo

142

}PTFE Syringe FiltersStrong, hydrophobic membrane is resistant to nearly all solvents, acids and bases


}Membrane: HPLC certified PTFE, with polypropylene support

}Housing: medical grade, solvent-resistant, virgin polypropylene

}Prefilter: binder-free glass microfiber

}Connections: enhanced female Luer-Lok inlet, male Luer slip outlet




Applications:

• Filtration of aggressive organic, highly basic or hot solutions, transducer protectors

• Filter aqueous solutions after prewetting with an alcohol

PVDF Syringe FiltersPerfect for general biological filtration and filtration of samples where high protein recovery is desired


}Membrane: HPLC-certified PVDF

}Hydrophilic membrane with good solvent resistance

}Housing: medical grade, virgin polypropylene

}Prefilter: binder-free glass microfiber prefilter





Applications:

• General biological filtration

• Filtration of samples where high protein recovery is desired

National Scientific Target PTFE Syringe FiltersOutside Dia. Pore Size Prefilter Cat. No. Quantity4mm 0.45µm No F2504-3 100 Pack4mm 0.2µm No F2504-4 100 Pack17mm 0.45µm No F2513-3 100 Pack17mm 0.2µm No F2513-4 100 Pack30mm 0.45µm No F2500-3 100 Pack30mm 0.2µm No F2500-4 100 Pack30mm 1.0µm No F2500-13 100 Pack30mm 0.45µm Yes F2502-3 100 Pack

National Scientific Target PVDF Syringe FiltersOutside Dia. Pore Size Prefilter Cat. No. Quantity4mm 0.45µm No F2504-5 100 Pack4mm 0.2µm No F2504-6 100 Pack17mm 0.45µm No F2513-5 100 Pack17mm 0.2µm No F2513-6 100 Pack30mm 0.45µm No F2500-5 100 Pack30mm 0.2µm No F2500-6 100 Pack

536_Page 142-143.indd 142 12/10/2009 6:43:07 PM

creo


Sample Preparation

Regenerated Cellulose Syringe FiltersIdeal for low nonspecific binding applications; tissue culture media filtration and general biological sample filtration


}Membrane: HPLC Certified Regenerated Cellulose

}Hydrophilic membrane with good solvent resistance; compatible with nearly all common HPLC solvents


}Protein binding: <5µg/cm2





Applications:

• Low nonspecific binding applications

• Tissue culture media filtration and general biological sample filtration

PES (Polyethersulfone) Syringe FiltersLow protein binding, PES membrane provides high flowrates with good throughput volume


}Membrane: ICP Certified PES (Polyethersulfone)






Applications:

• Ion chromatography

• Tissue culture filtration, filtration of proteins and nucleic acids

• High-temperature liquids

National Scientific Target Regenerated Cellulose Syringe FiltersOutside Dia. Pore Size Prefilter Cat. No. Quantity4mm 0.45µm No F2504-7 100 Pack4mm 0.2µm No F2504-8 100 Pack17mm 0.45µm No F2513-7 100 Pack17mm 0.2µm No F2513-8 100 Pack30mm 0.45µm No F2500-7 100 Pack30mm 0.2µm Yes F2500-8 100 Pack

National Scientific Target PES (Polyethersulfone) Syringe FiltersOutside Dia. Pore Size Prefilter Cat. No. Quantity17mm 0.45µm No F2513-14 100 Pack17mm 0.2µm No F2513-17 100 Pack30mm 0.45µm No F2500-14 100 Pack30mm 0.2µm No F2500-17 100 Pack

536_Page 142-143.indd 143 12/10/2009 6:43:08 PM

creo

144

}GMF (Glass MicroFiber) Syringe FiltersLarger porosity membrane; able to remove large particulates without clogging


}Membrane: Binder-free Glass Microfiber




}Max. operating pressure: 30mm - 90psi

}Retention volumes: 30mm - <137µL

Applications:

• Dissolution testing

• General filtration

Polypropylene Syringe FiltersPolypropylene membrane offers wide chemical compatibility with organic solvents


}Membrane: Hydrophilic Polypropylene

}Low nonspecific protein binding




}Max. operating pressure: 17mm - 115psi, 30mm - 90psi

}Retention volumes: 17mm - <29µL, 30mm - <137µL

Applications:

• Filtration of biological samples

• Filtration of aggressive organic solutions

National Scientific Target GMF (Glass MicroFiber) Syringe FiltersOutside Dia. Pore Size Prefilter Cat. No. Quantity30mm 0.7µm No F2500-18 100 Pack30mm 1.2µm No F2500-19 100 Pack30mm 3.1µm No F2500-20 100 Pack

National Scientific Target Polypropylene Syringe FiltersOutside Dia. Pore Size Prefilter Cat. No. Quantity4mm 0.45µm No F2504-9 100 Pack4mm 0.2µm No F2504-10 100 Pack17mm 0.45µm No F2513-9 100 Pack17mm 0.2µm No F2513-10 100 Pack30mm 0.45µm No F2500-9 100 Pack30mm 0.2µm No F2500-10 100 Pack30mm 0.45µm Yes F2502-9 100 Pack

536_Page 144-145.indd 144 12/10/2009 6:44:22 PM

creo


Sample Preparation

Cellulose Acetate Syringe FiltersLow protein binding membrane ideal for aqueous-based samples.


}Membrane: HPLC Certified Cellulose Acetate


}Protein binding: <24µg/cm2





Applications:

• Tissue culture media filtration, sensitive biological samples

National Scientific Target Cellulose Acetate Syringe FiltersOutside Dia. Pore Size Prefilter Cat. No. Quantity4mm 0.45µm No F2504-15 100 Pack4mm 0.2µm No F2504-16 100 Pack17mm 0.45µm No F2513-15 100 Pack17mm 0.2µm No F2513-16 100 Pack30mm 0.45µm No F2500-15 100 Pack30mm 0.2µm No F2500-16 100 Pack

536_Page 144-145.indd 145 12/10/2009 6:44:52 PM

creo

146

750µL Micro-Centrifugal Filters, NonsterileFilter volumes as low as 50µL with low hold-up volume

}Filter volumes as low as 50µL up to 750µL with low hold-up volume

}Use with any laboratory microcentrifuge

}Virgin polypropylene filter housing with tapered 2mL, capped receiver tube

}10,000xG maximum centrifugal force

All-Plastic Disposable SyringesDisposable syringes with polyethylene barrels and polypropylene plungers; use for all syringe filter applications

}Two-part, all-plastic construction eliminates the need for rubber or synthetic plunger gaskets

}No silicone or oil lubricant is required in the barrel

}Choose Luer-Slip or Luer-Lok syringes, in capacities ranging from 1 to 50mL

Nonsterile; packed in bulk.

National Scientific Target Target All-Plastic Disposable SyringesCapacity Cat. No. Quantity Luer-Slip Syringes1mL S7510-1 100 Pack3mL S7510-3 100 Pack5mL S7510-5 100 Pack10mL S7510-10 100 Pack20mL S7510-20 100 Pack30mL S7510-30 50 Pack50mL S7510-50 30 PackLuer-Lok Syringes3mL S7515-3 100 Pack5mL S7515-5 100 Pack10mL S7515-10 100 Pack20mL S7515-20 100 Pack

National Scientific 750µL Micro-Centrifugal Filters, NonsterileMaterial [Membrane] Pore Size Cat. No. QuantityCellulose Acetate 0.22µm F2517-1 100 PackCellulose Acetate 0.45µm F2517-2 100 PackNylon 0.2µm F2517-3 100 PackNylon 0.45µm F2517-4 100 PackPVDF 0.2µm F2517-5 100 PackPVDF 0.45µm F2517-6 100 PackRegenerated Cellulose 0.2µm F2517-7 100 PackRegenerated Cellulose 0.45µm F2517-8 100 PackPTFE 0.2µm F2517-9 100 PackPTFE 0.45µm F2517-10 100 Pack

536_Page 146-147.indd 146 12/10/2009 6:46:21 PM

creo


Sample Preparation

2mL Centrifugal Filters, Nonsterile}Filter sample volumes up to 2mL

}Virgin polypropylene filter housing with tapered 5mL, capped receiver tube

}Use with benchtop or floor model centrifuges


25mL Centrifugal Filters, Nonsterile}Filter sample volumes up to 25mL

}Virgin polypropylene filter housing with conical receiver

}Use with benchtop or floor model centrifuges


National Scientific 2mL Centrifugal Filters, NonsterileMaterial Pore Size Cat. No. QuantityCellulose Acetate 0.22µm F2520-1 25 PackCellulose Acetate 0.45µm F2520-2 25 PackNylon 0.2µm F2520-3 25 PackNylon 0.45µm F2520-4 25 PackPVDF 0.2µm F2520-5 25 PackPVDF 0.45µm F2520-6 25 PackPTFE 0.2µm F2520-7 25 PackPTFE 0.45µm F2520-8 25 Pack

National Scientific 25mL Centrifugal Filters, NonsterileMaterial Pore Size Cat. No. QuantityCellulose Acetate 0.22µm F2519-1 50 PackCellulose Acetate 0.45µm F2519-2 50 PackNylon 0.22µm F2519-3 50 PackNylon 0.45µm F2519-4 50 PackPVDF 0.22µm F2519-5 50 PackPVDF 0.45µm F2519-6 50 Pack

536_Page 146-147.indd 147 12/10/2009 6:46:25 PM

creo

Chemical Nylon PTFE PVDF PES CA RC PP GMF

ACIDS

Acetic, Glacial LC C C C IC C C C

Acetic, 25% C C C C CA C C C

Hydrochloric, Concentrated IC C C C IC IC C C

Hydrochloric, 25% IC C C C IC IC C C

Sulfuric, Concentrated IC C IC IC IC IC C C

Sulfuric, 25% IC C C C IC LC C C

Nitric, Concentrated IC C C IC IC IC C LC

Nitric, 25% IC C C C IC IC C LC

Phosphoric, 25% IC C ND ND C LC C ND

Formic, 25% IC C ND ND LC C C C

Trichloroacetic, 10% IC C ND ND C C C ND

ALCOHOLS

Methanol, 98% C C C C C C C C

Ethanol, 98% C C C C C C C C

Ethanol, 70% LC C C C C C C C

Isopropanol C C C C C C C C

n-Propanol C C C C C C C C

Amyl Alcohol (Butanol) C C C C C C C C

Benzyl Alcohol C C C ND LC C C IC

Ethylene Glycol C C C C C C C C

Propylene Glycol C C C C LC C C C

Glycerol C C C C C C C C

ALKALIES

Ammonium Hydroxide, 25% C C LC C C LC C C

Sodium Hydroxide, 3N C C C C IC LC C IC

AMINES AND AMIDES

Dimethyl Formamide LC C IC IC IC LC C C

Diethylacetamide C C ND ND IC C ND C

Triethanolamine C C ND ND C C ND ND

Aniline ND C ND ND IC C ND ND

Pyridine C C IC IC IC C IC C

Acetonitrile C C C LC IC C C C

LEGENDC = CompatibleLC = Limited Compatibility (Membrane may swell and shrink)IC = Incompatible (Not Recommended)ND = No Compatibility Data Currently AvailablePTFE = Polytetrafluoroethylene (Teflone)PVDF = PolyvinylidenePES = PolyethersulfoneCA = Cellulose AcetateRC = Regenerated CellulosePP = PolypropyleneGMF = Glass MicroFiber

Use the information in this table to determine the ability of a specific syringe filter membrane to withstand exposure to a solvent. All concentrations are 100% unless noted.

Syringe Filter Membrane Compatibility Chart

148148

536_Page 148-149.indd 148 12/10/2009 6:47:42 PM

creo

Chemical Nylon PTFE PVDF PES CA RC PP GMF

ESTERS

Ethyl Acetate/Methyl Acetate C C C IC IC C LC C

Amyl Acetate/Butyl Acetate C C IC IC LC C LC C

Propyl Acetate C C IC IC LC C LC ND

Propylene Glycol Acetate ND C ND IC IC C C ND

2-Ethoxyethyl Acetate ND C ND IC LC C ND ND

Methyl Cellusolve ND C ND IC IC C C C

Benzyl Benzoate C C ND IC C C ND ND

Isopropyl Myristate C C ND IC C C ND ND

Tricresyl Phosphate ND C ND IC C C ND ND

HALOGENATED HYDROCARBONS

Methylene Chloride LC C C IC IC C LC C

Chloroform C C C IC IC C LC C

Trichloroethylene C C C IC C C C C

Chlorobenzene C C C LC C C C C

Freon® C C C LC C C C C

Carbon Tetrachloride C C C IC LC C LC C

HYDROCARBONS

Hexane/Xylene C C C IC C C IC C

Toluene/Benzene C C C IC C C IC C

Kerosene/Gasoline C C C LC C C LC ND

Tetralin/Decalin ND C C ND C C ND ND

KETONES

Acetone C C IC IC IC C C C

Cyclohexanone C C IC IC IC C C C

Methyl Ethyl Ketone C C LC IC LC C LC C

Isopropylacetone C C IC IC C C ND C

Methyl Isobutyl Ketone ND C LC IC ND C LC C

ORGANIC OXIDES

Ethyl Ether C C C C C C LC ND

Dioxane C C LC IC LC C C C

Tetrahydrofuran C C LC IC LC C C C

Triethanolamine C C ND ND C C ND ND

Dimethylsulfoxide (DMSO) C C IC IC LC C C C

Isopropyl Ether ND C C C C C C ND

MISCELLANEOUS

Phenol, Aqueous Solution, 10% ND C LC IC IC IC C C

Formaldehyde Aqueous Solution, 30% C C C C C LC C C

Hydrogen Peroxide, 30% C C ND ND C C ND ND

Silicone Oil/Mineral Oil ND C C C C C C C


Sample Preparation

536_Page 148-149.indd 149 12/10/2009 6:47:43 PM

creo

Product Selection according to Manufacturer Type:

Manufacturer 96 Round 96 Round 96 Square 96 Square 384 Square 384 Square Silicone Silicone/PTFE Silicone Silicone/PTFE Silicone/PTFE

Abgene WSM-2E WSM-2 CoStar WSM-2FBE WSM-2FB WSM-5E WSM-5 WSM-2 Greiner WSM-2FBE WSM-2FB WSM-3SXE WSM-3S WSM-5E WSM-5 WSM-2E WSM-2 Matrix WSM-2FBE WSM-2FB WSM-3SXE WSM-3S WSM-2E WSM-2 NUNC WSM-7E WSM-3SXE WSM-3S WSM-5E WSM-5Porvair WSM-2FBE WSM-2FB WSM-3SXE WSM-3S WSM-5E WSM-5 WSM-2E WSM-2 Whatman WSM-2FBE WSM-2 WSM-3SXE WSM-3S WSM-2E

Chromacol WebSeal Product Selection:

The Chromacol WebSeal system is a comprehensive range of 96-well titer plates with glass inserts and a silicone/PTFE coated sealing mat. This system provides the analyst with 96 completely inert chambers and reduces the risk of cross-contamination from well to well when removing the cover. The products are ideal for High Throughput Screening, Combinatorial Chemistry, Life Science applications and HPLC.

WebSeal mats are typically blue or clear silicone rubber in nature. The clear mats have no PTFE coating, making them ideal for use with aqueous mobile phases.

Blue mats have a thin protective film of sprayed PTFE, making them ideal for use with organic solvents.

Mats are available in standard and pre-cut versions for delicate autosampler needles.

The addition of vials to the 96-well plates allows the use of aggressive solvents in the WebSeal system.

Products are available in volumes from 500µL to 1.5mL.

150

536_Page 150-151.indd 150 12/10/2009 6:49:47 PM

creo


Sample Preparation

WebSeal 500µL Kit and AccessoriesMid-depth polypropylene 96-well microtiter plate, pre-inserted 500µL glass vials and WebSeal silicone/PTFE mats

}Manufactured to a standard 96-Well footprint and compatible with all well-plate autosamplers

WebSeal 700µL Kit and Accessories700µL kits available with clear glass, amber glass and PTFE vials pre-inserted into 96-Well polypropylene microtiter plates

}Cutting tool included for vial removal

Chromacol WebSeal 700µL KitDescription Cat. No. Quantity96-well plate, 700µL clear glass vials, sealing mat and cutting tool MTPVC-96 5 Pack96-well plate, 700µL amber glass vials, sealing mat and cutting tool MTPVCA-96 5 Pack96-well plate, 700µL PTFE vials, sealing mat and cutting tool MTPTC-96 1 EachAccessories700µL clear glass vials for kit 1-MTV-96 500 Pack700µL amber glass vials for kit 1-MTV(A)-96 500 Pack700µL PTFE vials for kit 1-MTTV-96 100 Pack96-well polypropylene plate for 700µL vials MTP-96 5 Pack96 round well silicone/PTFE sealing mat WSM-1 5 Pack96 round well silicone/PTFE sealing mat, pre-slit WSM-1X 5 PackCutting tool MTPC-1 1 Each

Chromacol WebSeal 500µL KitDescription Cat. No. Quantity96-well microtiter plate with 500µL glass vials and sealing mats 05-MTPVC-96 5 PackAccessories96-well mid-depth polypropylene plate for 500µL vials 05-MTP-96 5 Pack500µL clear glass replacement vials for kit 05-MTV-96 500 Pack96 round well silicone/PTFE sealing mat WSM-1 5 Pack96 round well silicone/PTFE sealing mat, pre-slit WSM-1X 5 Pack

536_Page 150-151.indd 151 12/10/2009 6:49:59 PM

creo

152

WebSeal 1.1mL Kit and Accessories1.1mL kits available as 8mm crimp top standard vials or with WebSeal silicone/PTFE sealing mats

}Manufactured to a standard 96-Well footprint and compatible with all well plate autosamplers

WebSeal 1.5mL Kit and Accessories1.5mL kit available in 96-well polypropylene microtiter plate pre-inserted with 1.5mL clear glass vials and WebSeal silicone/PTFE sealing mats

}Manufactured to a standard 96-Well footprint and compatible with most well-plate autosamplers

Chromacol WebSeal 1.1mL Kit and AccessoriesDescription Cat. No. Quantity96-well PP plate with 1.1mL glass vials, sealing mats 1.1-MTPVC-96 5 Pack96-well PP plate with 1.1mL, 8mm crimp top vials 1.1-CMTPVC-96 5 PackAccessories1.1mL clear glass vials for kit 1.1-MTV-96 500 Pack1.1mL clear glass crimp top vials for kit 1.1-CRV 500 Pack96 deep well PP plate for 1.1mL shell vial 1.1-MTP-96 5 Pack96 square deep well PP plate for 1.1mL crimp top vials 1.1-MTPS-96 5 PackSilicone/PTFE sealing mat WSM-6 5 PackSilicone/PTFE sealing mat pre-slit WSM-6X 5 Pack

Chromacol WebSeal 1.5mL Kit and AccessoriesDescription Cat. No. Quantity96 well polyproylene plate, 1.5mL glass vials, sealing mat 1.5-MTPVC-96 5 PackAccessories1.5mL clear glass vials for kit 1.5-MTV-96 500 Pack96 well polyproylene plate for 1.5mL glass vials 1.5-MTP-96 5 PackSilicone/PTFE sealing mat WSM-6 5 PackSilicone/PTFE sealing mat pre-slit WSM-6X 5 Pack

536_Page 152-153.indd 152 12/10/2009 6:52:00 PM

creo


Sample Preparation

MicroMat CLR Sealing MatsDesigned to fit 96-well plates to eliminate cross-contamination from well to well

}Manufactured from pure silicone

}Wider penetration area for ease of use

}Dry heat autoclavable

}Resists coring or tearing

}Available pre-slit to reduce vacuum formation

WebSeal MicroMat CLR Sealing StripsUse where only partial plate capacity is required

}Available pre-slit for easy penetration and reduced vacuum formation

Chromacol MicroMat CLR Sealing MatsDescription Cat. No. Quantity96 round well; 7mm domed base WSM-2E 5 Pack96 round well sealing mat, 7mm domed base, silicone only pre-slit WSM-2XE 5 Pack384 square well sealing mat, silicone only WSM-5E 5 Pack384 square well sealing mat, silicone only pre-slit WSM-5XE 5 Pack96 square well sealing mat, silicone only WSM-3SE 5 Pack96 square well sealing mat, silicone only pre-slit WSM-3SXE 5 Pack96 round well sealing mat, 8mm flat base, silicone WSM-2FBE 5 Pack96 round well sealing mat, 8mm flat base, silicone pre-slit WSM-2FBXE 5 Pack

Chromacol WebSeal MicroMat CLR Sealing StripsDescription Cat. No. Quantity96 round well sealing strips; 8mm strips; silicone pre-slit WSMS-2XE 12 Pack

536_Page 152-153.indd 153 12/10/2009 6:52:02 PM

creo

154

WebSeal MatsSuperior resealability after multiple injections

}Excellent chemical compatibility


}Eliminates cross-contamination well to well


WebSeal Mat ApplicatorHandheld applicator for easy sealing of WebSeal mats

WebSeal Silicone/PTFE MatsManufactured from silicone with PTFE coating on underside

}Superior resealability after multiple injections

}Excellent chemical compatibility


}Eliminates cross-contamination well to well


Chromacol WebSeal MatsDescription Cat. No. Quantity96 round well silicone/PTFE sealing mat WSM-1 5 Pack96 round well silicone/PTFE sealing mat, pre-slit WSM-1X 5 PackSilicone/PTFE sealing mat WSM-6 5 PackSilicone/PTFE sealing mat pre-slit WSM-6X 5 Pack

Chromacol WebSeal Mat ApplicatorDescription Cat. No. QuantityHandheld mat applicator WSA-1 1 Each

Chromacol WebSeal Silicone/PTFE MatsDescription Cat. No. Quantity96 round well; 7mm dome base; Dark Blue WSM-2 5 Pack96 round well; 7mm flat base; Light Blue WSM-2FB 5 Pack96 round well; 7mm flat base; Light Blue, pre-slit WSM-2FBX 5 Pack96 square well; 8mm square well; Light Blue WSM-3S 5 Pack96 square well; 8mm square well; Light Blue, pre-slit WSM-3SX 5 Pack96 square well; 8mm square well; Yellow, pre-slit WSM-3SXY 5 Pack384 round well; 4mm square well; Red WSM-5 5 Pack384 square well; 4mm square well; Light Blue, pre-slit WSM-5RX 5 Pack

536_Page 154-155.indd 154 12/10/2009 6:53:16 PM

creo


Sample Preparation

WebSeal Glass-Coated MicroplatesLightweight, precision molded, cost-effective alternative to solid glass plates

}Polypropylene microplates coated with 200nm thick layer of silicone dioxide

}Chemically resistant with the qualities of glass and advantages of polypropylene

}Operating temperatures of -80° to +80°C

Mini-Vap Sample ConcentratorEvaporates 500µL methanol in less than 10 minutes

}Evaporating 500µL methanol in 6 minutes

WebSeal UltraVap High-Speed Sample ConcentratorProgrammable high speed sample concentrator with patented spiral needles

Chromacol Mini-Vap Sample ConcentratorDescription Cat. No. QuantityMinivap Sample Concentrator, Spiral Needle Design (110 volt) CLS-229203 1 Each

Chromacol WebSeal UltraVap High Speed Sample ConcentratorDescription Cat. No. QuantityUltraVap 96 Well High Speed Sample Concentrator CLS-229070 1 EachReplacement Spiral Needle Kit with Fitting Tool CLS-229074 1 Each

Chromacol WebSeal Glass-coated MicroplatesDescription Cat. No. Quantity96 U deep-well design; 1mL glass-coated PP plate CLS-400042 10 Pack96 flat well design; 2mL glass-coated PP plate CLS-400046 10 Pack96 V-design 190µL; Glass coated PP plate CLS-400058 10 Pack384 square-rounded; 180µL glass-coated PP plate CLS-400156 10 Pack96 U-well design; 250µL glass-coated PP plate CLS-400054 10 Pack96 well flat bottom; 300µL glass-coated PP plate CLS-400062 10 Pack384 square/rounded well design; 90µL glass-coated PP plate CLS-400150 10 Pack

536_Page 154-155.indd 155 12/10/2009 6:53:23 PM

creo

156

The following section provides properties of the WebSeal product range:

Physical Properties and Resistance:

Low High

Operating Temperature -80ºC 260ºCSpecific Gravity 1.15Shore Hardness 57Tear Strength Fair

WebSeal Sealing Mat Properties:

Material Properties

Blue Silicone/PTFE Mat Soft silicone with sprayed clear PTFE layer to give resistance against a wide range of organic solvents. Suitable for injection with a wide range of autosampler and sample processing units. Pre-slit versions allow use of blunt probes and pipette tipsClear Silicone Mat Soft silicone without protective spray. Cannot be used for extended periods with strong solvents. May be used under aqueous conditions for storage

WebSeal Chemical Resistance:

The chemical resistance has been tested with long-term exposure of mats to filled plates with measurement of solvent integrity and observation of mat sealing performance over periods of 2-3 months.

Resistance to attack and application suitability

PTFE coated for Glass Vials

PTFE coated for square or round well plates

Pre-Slit PTFE coated for square or round well plates

Solvents

No permeation of mat elastomer or mat coating. Suitable for long-term storage, sample transfer & autosampler use

Excellent Excellent Excellent Water & Inorganic BuffersMethanol/WaterIPAAcetonitrile/WaterHexaneHeptaneCyclohexane

No permeation of mat elastomer by solvent with intact PTFE surface. Suitable for sample transfer and autosampler use. Suitable for elevated temperature use

Excellent under most conditions



AcetoneAcetonitrileDichloromethane (DCM)Dimethylformamide (DMF)Trifluoroacetic Acid

Permeation of the elastomer after 3-5 days may lead to solvent collection on upper face of mat. Suitable for short-term sample transfer and autosampler use only

Fair but alternative may be preferred

Not recommended for this application

Not recommended for this application

Dimethylsulphoxide (DMSO)

Results:

Temperature Solvent % loss after 24 hours

-20ºC Water 0.15% Acetonitrile 12.0% DMSO -1.1%4ºC Water 0.4% Acetonitrile 19.2% DMSO 0.8%20ºC Water 1.0% Acetonitrile 34.3% DMSO -1.2%

Evaporation Study:

An evaporation study was performed using water, acetonitrile and DMSO at two different volumes (100µL and 200µL) at three different temperatures (28ºC, 4ºC and -20ºC for 24 hours). Plates were filled with solvent, weighed, then sealed with MicroMats. The sealed plates were incubated at their respective temperatures for 24 hours. Mats were removed and the plates were reweighed to determine evaporation of sample content.

WebSeal Technical Information:

Sterilization:

WebSeal may be sterilized but is produced in a chromatographically clean form and is suitable for use straight from the packet.

Property Method Comment

Sterilization methods Autoclave 160ºC recommended Chemical All common disinfectants may be suitable Radiation Gamma

536_Page 156-157.indd 156 12/10/2009 6:55:15 PM

creo


Sample Preparation

Notes

536_Page 156-157.indd 157 12/10/2009 6:55:15 PM

creo

Thermo ScientificChromatography Columns and Consumables The right tools, separation after separation.

2010/2011}

2010201 1

Chromatography Colum

ns and Consumables

North America

USA and Canada800 332 3331865 354 4616 [email protected] Europe

France +33 (0) 3 88 67 53 20+33 (0) 3 88 67 11 68 [email protected] Germany+49 6103 408 0+49 6103 408 1111 [email protected]

Switzerland+41 56 618 41 11 +41 56 618 41 41 [email protected]

United Kingdom+44 1509 555500+44 1509 555111 [email protected]

Asia

Japan+81 45 453 9220+81 45 453 9226 [email protected]

China800-810-5118Shanghai: 86-21-64457830 faxBeijing: 86-1084193583 faxGuangzhou: 86-20-83486621 [email protected] India1800 22 8374 (toll-free)+91 22 6716 2200+91 22 6716 2244 [email protected]

All Other Enquiries

+44 (0) 1928 534 050+44 (0) 1928 534 049 [email protected]

© 2009 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.

CTGSCCHROMCAT 0110

For more information, please visit our website at www.thermoscientific.com/chromatography

How to Order

Resources for ChromatographersBeyond this catalog, our Chromatography team shares its extensive expertise through our web-based Chromatography Resource Center and the Separated by Experience enewsletter.

The Chromatography Resource Center, accessed at www.thermoscientific.com/chromatography provides technical support, applications, technical tips and literature to help you move your separations forward, quickly and easily.

The bi-monthly Separated by Experience enewsletter keeps you up-to-date on the latest technical and product information of interest to chromatographers. Valuable information developed by chromatographers for chromatographers. Subscribe today at www.thermoscientific.com/chromatography

Technical SupportNorth America800 332 [email protected]

Outside North America+44 (0) 1928 534 [email protected]

ThermoScientific_CVR_IFC_IBC_BC_FINAL.indd 1 12/7/2009 5:20:15 PM

asia europe chromatography all other enquiriesimg43.chem17.com/5/20100814/634173864745745345.pdf ·...

Documents