Supplementary Information

Comparative Embryotoxicity of Phenanthrene and

Alkyl-phenanthrene to Marine Medaka (Oryzias

melastigma)

Jingli MU 1,2,3, Juying WANG 1,2, Fei JIN 1,2, Xinhong WANG 3 , Huasheng HONG 3

1. Key Laboratory for Ecological Environment in Coastal Areas，State Oceanic Administration, Dalian 116023,

China;

2. Division of Marine Chemistry, National Marine Environmental Monitoring Center, Dalian 116023, China;

3. State Key Laboratory of Marine Environmental Science, Environmental Science Research Center, Xiamen

University, Xiamen 361005, China.

Control Phenanthrene 200 µg/L

Phenanthrene 800 µg/L Phenanthrene 400 µg/L

Retene 200 µg/L Retene400 µg/L

Retene 400 µg/L Retene 800 µg/L

Fig. S1. Developmental toxicity and BSD signs scoring of phenanthrene and retenen on marine medaka embryos. Body hemorrhaging (BH) was scored according to yes or no (0–1); pericardial edema (PE) was scored according to the ratio of edema fluid volume to intracapsular volume (0–4); yolk sac edema(YE) was scored according to the ratio of edema fluid volume to capsule volume (0–3); stretched heart (SH) was scored according to the stretched degree of heart (0–2); circulation reduction (CR) was scored according to blood flow velocity (0–3).

Control

Phenanthrene 200 µg/L Phenanthrene 400 µg/L Phenanthrene 800 µg/L

Phenanthrene 800 µg/LPhenanthrene 400 µg/LPhenanthrene 200 µg/L

Retene 400 µg/LRetene 100 µg/L Retene200 µg/L

Retene 200 µg/LRetene 100 µg/LRetene 100 µg/L Retene 400 µg/L

Phenanthrene 400 µg/L Phenanthrene 800 µg/LPhenanthrene 200 µg/L

Ret 200 µg/LRet 100 µg/L Ret 400 µg/L

Fig. S2. Developmental toxicity of phenanthrene and retene on marine medaka larvae.

Fig. S3. CYP1A protein expression of marine medaka embryos exposed to phenanthrene and retene for 8 d and 11 d, n=10~30 embryos/larvae.