arginine stimulation test for ghrh

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GHRH-Arginine stimulation test INDICATION Used to confirm GH deficiency: 1. In transition, after achievement of final height, to confirm persistence of childhood GH deficiency in late adolescence/early adulthood if insulin tolerance test is contraindicated or patient is overweight or has diabetes mellitus which may limit interpretation of insulin tolerance test or glucagon stimulation test. 2. In adults, if glucagon stimulation test or insulin tolerance test shows GH deficiency (peak GH <5 mcg/L), and 2nd confirmatory test is needed before commencing GH treatment, especially if overweight or ITT contraindicated such as after traumatic brain injury. PREPARATION Fasting overnight from midnight (except water) Arrive by 9 am At least 1 month off GH treatment if in transition. 7 red top Vacutainers METHOD 1) Cannulate in both arms, and leave to rest for 45 minutes. 2) Take blood into a plain tube (red top Vacutainer) for baseline GH and IGF-1 measurement (-15 mins), and GH at 0 min. 3) Inject GHRH (Somatorelin, Ferring) 1mcg / kg (maximum dose 100 mcg) as bolus injection. 4) Infuse 0.5 g / kg L-arginine monohydrochloride (maximum dose 30 g) as a 10% solution (30 g / 300 mL)

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GHRH-Arginine stimulation testINDICATION

Used to confirm GH deficiency:

1. In transition, after achievement of final height, to confirm persistence of childhood GH deficiency in late adolescence/early adulthood if insulin tolerance test is contraindicated or patient is overweight or has diabetes mellitus which may limit interpretation of insulin tolerance test or glucagon stimulation test.2. In adults, if glucagon stimulation test or insulin tolerance test shows GH deficiency (peak GH 21y), GH deficiency depends on peak GH and BMI:

BMI 30 Peak GH< 4 mcg/L

In transition (adolescence and young adults