aquasentinel: a real-time reagentless biosensor system for ... · oak ridge national laboratory oak...
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OAK RIDGE NATIONAL LABORATORYU.S. DEPARTMENT OF ENERGY
Elias Greenbaum and Miguel Rodriguez, Jr.Chemical Sciences Division
Oak Ridge National LaboratoryOak Ridge, Tennessee
1
AquaSentinel:A Real-Time Reagentless Biosensor System for Standoff Detection and Classification of
Toxins in Source Water
NWQMCSixth National Monitoring Conference
May 18-22, 2008Atlantic City, New Jersey
Features of AquaSentinel• Extracts information from minute changes in a
natural aqueous environment• No sample preparation• Sediment, debris okay• Sentinel + Chemical Identification• Standoff-hands off detection• No consumable reagents• Electromagnetically silent until an event has
occurred• Mobile; Direct line of sight is not required• Wireless encrypted bidirectional telecommunication• Broad spectrum of aqueous toxins• Small size; Lab-on-a-chip capable
KCN DCMU Atrazine0 50 100 150 200 250 300 350 400 450 500
0.6
0.7
0.8
0.9
1
1.1
1.187
0.699
Trans n
4800 n0 100 200 300 400 500
0.6
0.8
1
1.2
1.4
1.6
1.8
1.837
0.783
Trans k
4800 k
0 50 100 150 200 250 300 350 400 450 50.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
0 100 200 300 400 5000.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat0 100 200 300 400 500
0.7
0.8
0.9
1
1.1
1.11
0.771
Trans n
5000 n
Methyl Parathion
KCN DCMU Atrazine0 50 100 150 200 250 300 350 400 450 500
0.6
0.7
0.8
0.9
1
1.1
1.187
0.699
Trans n
4800 n0 100 200 300 400 500
0.6
0.8
1
1.2
1.4
1.6
1.8
1.837
0.783
Trans k
4800 k
0 50 100 150 200 250 300 350 400 450 50.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
0 100 200 300 400 5000.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat0 100 200 300 400 500
0.7
0.8
0.9
1
1.1
1.11
0.771
Trans n
5000 n
0 50 100 150 200 250 300 350 400 450 5000.6
0.7
0.8
0.9
1
1.1
1.187
0.699
Trans n
4800 n0 100 200 300 400 500
0.6
0.8
1
1.2
1.4
1.6
1.8
1.837
0.783
Trans k
4800 k
0 50 100 150 200 250 300 350 400 450 50.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
0 100 200 300 400 5000.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat0 100 200 300 400 500
0.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat0 100 200 300 400 500
0.7
0.8
0.9
1
1.1
1.11
0.771
Trans n
5000 n
Methyl Parathion
0 2 4 6 8 100.06
0.08
0.1
0.12
0.14
0.16
0.18176
076
rol
N D5+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.283
.05
Control
MPt Δ+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.278
0.061
Control
DCMU D−
100 t
0 2 4 6 8 100.1
0.15
0.2
0.25
0.264
0.112
Control
Paraquat D+
100 t
0 2 4 6 8 100.2
0.3
0.4
0.5
0.6
0.70.64
0.253
Control
Atrazine D−
100 t
KCN DCMU Atrazine
Methyl ParathionParaquat
0 2 4 6 8 100.06
0.08
0.1
0.12
0.14
0.16
0.18176
076
rol
N D5+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.283
.05
Control
MPt Δ+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.278
0.061
Control
DCMU D−
100 t
0 2 4 6 8 100.1
0.15
0.2
0.25
0.264
0.112
Control
Paraquat D+
100 t
0 2 4 6 8 100.2
0.3
0.4
0.5
0.6
0.70.64
0.253
Control
Atrazine D−
100 t
KCN DCMU Atrazine
Methyl ParathionParaquat0 2 4 6 8 10
0.05
0.1
0.15
0.2
0.25
0.283
.05
Control
MPt Δ+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.278
0.061
Control
DCMU D−
100 t
0 2 4 6 8 100.1
0.15
0.2
0.25
0.264
0.112
Control
Paraquat D+
100 t
0 2 4 6 8 100.2
0.3
0.4
0.5
0.6
0.70.64
0.253
Control
Atrazine D−
100 t
KCN DCMU Atrazine
Methyl ParathionParaquat
Effect on Fluorescence Digital Signature – “Fingerprint”
Basic Idea of AquaSentinel
CLINCH RIVER/MELTON HILL LAKE, OAK RIDGE, TN
AquaSentinel Monitoring Station
Drinking Water Supplyfor Oak Ridge, TN
Real-World Conditions
Clinch River Monitoring Station - ORNL
0 2 4 6 8 100.3
0.4
0.5
0.6
0.7
0.80.736
0.316
Control
Atrazine
100 t0 2 4 6 8 10
0.2
0.3
0.4
0.5
0.6
0.70.64
0.253
Control
Atrazine D−
100 t
0 50 100 150 200 250 300 350 400 450 5000.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
Atrazine Data Analysis – 0.3 ppm
Transformation Data Set for Atrazine
0 2 4 6 8 100.3
0.4
0.5
0.6
0.7
0.80.736
0.316
Control
Atrazine
100 t0 2 4 6 8 10
0.2
0.3
0.4
0.5
0.6
0.70.64
0.253
Control
Atrazine D−
100 t
0 50 100 150 200 250 300 350 400 450 5000.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
Atrazine Data Analysis – 0.3 ppm
Transformation Data Set for Atrazine
0 50 100 150 200 250 300 350 400 450 5000.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
Transformation Data Set for Atrazine
0 2 4 6 8 100.1
0.15
0.2
0.25
0.3
0.350.332
0.109
Control
DCMU
100 t0 2 4 6 8 10
0.05
0.1
0.15
0.2
0.25
0.278
0.061
Control
DCMU D−
100 t
0 100 200 300 400 5000.6
0.8
1
1.2
1.4
1.6
1.8
1.837
0.783
Trans k
4800 k
DCMU Data Analysis – 2 ppm
Transformation Data Set for DCMU
0 100 200 300 400 5000.6
0.8
1
1.2
1.4
1.6
1.8
Trans k
k
Transformation Data Set for DCMU
0 2 4 6 8 100.1
0.15
0.2
0.25
0.264
0.103
Control
Paraquat
100 t
0 2 4 6 8 100.1
0.15
0.2
0.25
0.264
0.112
Control
Paraquat D+
100 t
0 100 200 300 400 5000.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat Data Analysis – 6 ppm
Transformation Data Set for Paraquat
0 100 200 300 400 5000.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Transformation Data Set for Paraquat
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.283
0.083
Control
MPt
100 t0 2 4 6 8 10
0.05
0.1
0.15
0.2
0.25
0.283
.05
Control
MPt Δ+
100 t
0 100 200 300 400 5000.7
0.8
0.9
1
1.1
1.11
0.771
Trans n
5000 n
Methyl Parathion Data Analysis – 5 ppm
Transformation Data Set for Methyl Parathion
0 100 200 300 400 5000.7
0.8
0.9
1
1.1
1.11
0.771
Trans n
5000 n
Transformation Data Set for Methyl Parathion
0 2 4 6 8 100.06
0.08
0.1
0.12
0.14
0.16
0.180.176
0.076
Control
KCN D5+
100 t
0 2 4 6 8 100.06
0.08
0.1
0.12
0.14
0.16
0.18
0.06
Control
KCN
100 t
0 0.02 0.04 0.060
0.1
0.2
0.3
0.4
0.50.406
0.08
Resk
0.050 Dk
D
0 50 100 150 200 250 300 350 400 450 5000.6
0.7
0.8
0.9
1
1.1
1.187
0.699
Trans n
4800 n
KCN Data Analysis – 130 ppm
Transformation Data Set for KCN
0 50 100 150 200 250 300 350 400 450 5000.6
0.7
0.8
0.9
1
1.1
1.187
0.699
Trans n
4800 n
Transformation Data Set for KCN
0 2 4 6 8 100.06
0.08
0.1
0.12
0.14
0.16
0.180.176
0.076
Control
KCN D5+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.283
.05
Control
MPt Δ+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.278
0.061
Control
DCMU D−
100 t
0 2 4 6 8 100.1
0.15
0.2
0.25
0.264
0.112
Control
Paraquat D+
100 t
0 2 4 6 8 100.2
0.3
0.4
0.5
0.6
0.70.64
0.253
Control
Atrazine D−
100 t
KCN DCMU Atrazine
Methyl ParathionParaquat
Summary of Toxin Data + Differential Offset Analysis
KCN DCMU Atrazine0 50 100 150 200 250 300 350 400 450 500
0.6
0.7
0.8
0.9
1
1.1
1.187
0.699
Trans n
4800 n0 100 200 300 400 500
0.6
0.8
1
1.2
1.4
1.6
1.8
1.837
0.783
Trans k
4800 k
0 50 100 150 200 250 300 350 400 450 5000.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
0 100 200 300 400 5000.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat0 100 200 300 400 500
0.7
0.8
0.9
1
1.1
1.11
0.771
Trans n
5000 n
Methyl Parathion
Summary of Toxin-Specific Transformation Data Sets
KCN DCMU Atrazine0 50 100 150 200 250 300 350 400 450 500
0.6
0.7
0.8
0.9
1
1.1
1.187
0.699
Trans n
4800 n0 100 200 300 400 500
0.6
0.8
1
1.2
1.4
1.6
1.8
1.837
0.783
Trans k
4800 k
0 50 100 150 200 250 300 350 400 450 50.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
0 100 200 300 400 5000.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat0 100 200 300 400 500
0.7
0.8
0.9
1
1.1
1.11
0.771
Trans n
5000 n
Methyl Parathion
KCN DCMU Atrazine0 50 100 150 200 250 300 350 400 450 500
0.6
0.7
0.8
0.9
1
1.1
1.187
0.699
Trans n
4800 n0 100 200 300 400 500
0.6
0.8
1
1.2
1.4
1.6
1.8
1.837
0.783
Trans k
4800 k
0 50 100 150 200 250 300 350 400 450 50.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
0 100 200 300 400 5000.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat0 100 200 300 400 500
0.7
0.8
0.9
1
1.1
1.11
0.771
Trans n
5000 n
0 50 100 150 200 250 300 350 400 450 5000.6
0.7
0.8
0.9
1
1.1
1.187
0.699
Trans n
4800 n0 100 200 300 400 500
0.6
0.8
1
1.2
1.4
1.6
1.8
1.837
0.783
Trans k
4800 k
0 50 100 150 200 250 300 350 400 450 50.8
0.9
1
1.1
1.2
1.31.274
0.853
Trans k
4800 k
0 100 200 300 400 5000.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat0 100 200 300 400 500
0.8
0.85
0.9
0.95
1
1.05
1.1
1.114
0.835
Trans k
4800 k
Paraquat0 100 200 300 400 500
0.7
0.8
0.9
1
1.1
1.11
0.771
Trans n
5000 n
Methyl Parathion
0 2 4 6 8 100.06
0.08
0.1
0.12
0.14
0.16
0.18176
076
rol
N D5+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.283
.05
Control
MPt Δ+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.278
0.061
Control
DCMU D−
100 t
0 2 4 6 8 100.1
0.15
0.2
0.25
0.264
0.112
Control
Paraquat D+
100 t
0 2 4 6 8 100.2
0.3
0.4
0.5
0.6
0.70.64
0.253
Control
Atrazine D−
100 t
KCN DCMU Atrazine
Methyl ParathionParaquat
0 2 4 6 8 100.06
0.08
0.1
0.12
0.14
0.16
0.18176
076
rol
N D5+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.283
.05
Control
MPt Δ+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.278
0.061
Control
DCMU D−
100 t
0 2 4 6 8 100.1
0.15
0.2
0.25
0.264
0.112
Control
Paraquat D+
100 t
0 2 4 6 8 100.2
0.3
0.4
0.5
0.6
0.70.64
0.253
Control
Atrazine D−
100 t
KCN DCMU Atrazine
Methyl ParathionParaquat0 2 4 6 8 10
0.05
0.1
0.15
0.2
0.25
0.283
.05
Control
MPt Δ+
100 t
0 2 4 6 8 100.05
0.1
0.15
0.2
0.25
0.278
0.061
Control
DCMU D−
100 t
0 2 4 6 8 100.1
0.15
0.2
0.25
0.264
0.112
Control
Paraquat D+
100 t
0 2 4 6 8 100.2
0.3
0.4
0.5
0.6
0.70.64
0.253
Control
Atrazine D−
100 t
KCN DCMU Atrazine
Methyl ParathionParaquat
Effect on Fluorescence Digital Signature – “Fingerprint”
Basic Idea of AquaSentinel
OAK RIDGE NATIONAL LABORATORYU.S. DEPARTMENT OF ENERGY
Acknowledgements
Charlene A. SandersMiguel Rodriguez, Jr.
Defense Advanced Projects Research AgencyUnited Defense, Inc./BAE Systems