Aquaculture: our golden future

Dr. Serge Corneillie (Alltech Japan-Korea-Philippines)Dr. Renato Kitajima (Alltech Japan, technical coordinator Aquaculture

Dr. Renato KitajimaPh.D student of Tokyo Marine University (Ph.D. thesis, sept 2013)3 months interim with Alltech Japan: study on the importance of DHA

NEW: SUSTAINABLE !!!

What

ARE WE BRINGING HEALTH THROUG FOOD? OUR MESSAGE?

European supermarkets (TESCO, Sainsbury, Mark and Spencer, Carrefour, …)

ALL WILD FISH: Marine Stewardship certified

FISH FARMED WITHOUT “ANY”FISH MEAL AND FISH OIL

Carrefour is committed to reducingthe environmental impact of its retail business.The Group sets great store by implementing initiatives to tackle climate change and preserve theplanet’s natural resources. It is striving to develop stores that use less energy and water, generateless waste and emit less CO2 and which have less of an impact on the environment all along thelogistics chain. The Group’s aims are clear: reduce its energy consumption per square metre ofsales area by 30% by 2020 (compared with 2004), reduce its greenhouse gas emissions by 40% by2020 (compared with 2009 in France, Belgium, Spain and Italy), eliminate HFCs from newrefrigeration equipment starting in 2015, reduce store water consumption and encourage therecycling of store waste. To do so, Carrefour is designing energy-saving stores, experimentingwith new technologies, sharing examples of best practice with other market players, raisingawareness among its staff and trying to get its customers

WILL JAPANESE SUPERMARKETS FOLLOW?

• Website of AEON: Environment and Social Initiatives report 2013

• STATEMENT:

• Selling MSC certified products to ensure that wild fish forever continues to reach our dinner tables

CAN WE? CAN WE DELIVER HEALTHY AND SUSTAINABLE FISH?CAN WE PRODUCE FISH WITHOUT USING ANY FM AND FO?

Effect of taurine, phytase and enzyme

complex supplementation to low fish meal

diet on growth of juvenile red sea bream

Pagrus major

S. Satoh1, oI. Hanini1, Md. S. A. Sarker1, Y. Haga1, H.

Nakayama2 ,S. Corneillie3 2010

1 Tokyo University of Marine Science and

Technology2 Bussan Biotech

3 Alltech Japan

Copyright 2011.Alltech

Ingredients FM50 FM20 FT FTP FTE 0.05 FTE 0.1

Anchovy meal 50 20 20 20 20 20

Soybean meal 0 18 18 18 18 18

Corn gluten meal 5 23 23 23 23 23

Fish oil 5 5 5 5 5 5

Soybean oil 5 7.4 7.4 7.4 7.4 7.4

Ca(H2PO4)2 1 1 1 1 1 1

Taurine - - 0.2 0.2 0.2 0.2

Phytase

(IU/g)- - - 1000 - -

Enzyme complex - - - - 0.05 0.1

Others* 34 25.6 25.4 25.2 25.35 25.3

Formula of the experimental diets (%)

Others*:starch,vitaminpremixture, mineral mix, wheat flour

Copyright 2011.Alltech

Copyright 2011.Alltech

0

10

20

30

40

50

60

70

80

0 4 8 12 16

FM 50 FM 20 FT FTP FTE 0.05 FTE 0.1

Time (Weeks)

We

igh

t (g

)Growth of fish during 12 weeks feeding trial

Copyright 2011.Alltech

Effect of replacing fish meal with a plant protein on growth performance, nutrient utilization and oxidative condition of Red sea bream, Pagrus major

Matsukura Kazuki, 2013

RSB grew better on a 0 % FM diet compared with a 50 % FM diet

The Japanese Society of Fisheries Science SpringMeeting 2013 ''Program and Abstracts''

Satoh, 2012

Copyright 2011.Alltech

Indoor growth trial with fish meal free feed and direct granulation EP (expanding) feed in yellow tail yearlings

Noriko Ishida (National Research Institute of Fisheries Science)Koji Maeno, Sadaetsu Yanagi (Kagoshima Prefectural Research Institute of Fisheries Science)Tomohiko Koshiishi (Nisshin Marubeni Feeds)Shuichi Satoh (Tokyo University of Marine Science and Technology)

Dietary treatments were 50% fish meal feed (Control diet), fish meal free EP feed with soy protein concentrate and bonito peptides (Diet 1),fish meal free EP feed with pork meal and bonito peptides (Diet 2) direct granulation EP feed with soy bean meal and raw anchovy (Diet 3).

Weight gain: 1058 1028 834 1210

Conclusion: No difference in performance

The Japanese Society of Fisheries Science Spring Meeting 2013 ''Program and Abstracts'‘Abstract 911: March, 27, 2013 p131

Copyright 2011.Alltech

Marine cage growth trial with fish meal free EP(expanding) feed in yellow tailsTatsuo Tsuzaki, Takurou Hotta, Kazunori Yoshida, Masahiro Nakagawa(West Research Institute of Fisheries Science)

Tomohiko Koshiishi (Nisshin Marubeni Feeds)Masayuki Yamada, Shuichi Satoh (Tokyo University of Marine Science and Technology)Noriko Ishida (National Research Institute of Fisheries Science)

50% fish meal feed (Control diet), fish meal free EP feed with soy protein concentrate and bonito peptides (Diet 1),fish meal free EP feed with pork meal and bonito peptides (Diet 2) EP feed with soy protein concentrate and liquid fish intestine (Diet 3).

Weight gain in 6 months:2242 2178 2205 2234

No difference in performance

The Japanese Society of Fisheries Science Spring Meeting 2013 ''Program and Abstracts'‘Abstract: March, 27, 2013 p131

Copyright 2011.Alltech

PS: Use of Aspergillus niger on top of

current diets!

YELLOWTAIL

TILAPIA

AMBERJACK

Copyright 2011.Alltech

Zero FO diets

Do Fish need HUFA’s? How much?Do People need them? How much?

Copyright 2011.Alltech

Recommendation of DHA /EPA intake for adults (mg/day)

Fatty acids in common foods in Japan (MEXT 2005)

Total FAg/100g

Saturatedg/100g

Monog/100g

PUFAg/100g

DHAmg/100g

EPAmg/100g

Beef (Sirloin) 23.05 9.14 13.29 0.62 0 0

Pork loin 17.73 7.84 7.68 2.21 10 0

Chicken Thigh 12.72 4.30 6.61 1.82 7 1

Milk 3.8 3.32 0.87 0.12 0 1

Canola oil 93.83 15.04 37.59 41.19 0 0

Sesame oil 93.26 7.06 60.09 26.10 0 0

Chicken egg 8.18 2.84 3.69 1.66 120 0

Algae (dried nori) 2.2 0.55 0.2 1.39 0 1200

Atlantic salmon 16.1 3.6 5.36 3.79 1400 850

Fruits, seeds, cereals, mushrooms, vegetables

0.1 - 73 0.1 - 12 0.1 - 59 0.1 - 41 0 0

DHA + EPA content in commercial fish in Japan

(MEXT 2005)

Most Fish is rich in DHA (in 2005!)

• 7.000.000.000 people a 250 mg /day

• 1.750 tons/day

• 650.000 tons/year

• Fish oil: 1 million tons

• DHA: 10 %; 100.000 tons

• ONL Y SIGNIFICANT SOURCE OF DHA IS FROM FISH OIL!!

• (50 million ton of salmon with present DHA level: 2 now)

DHA GLOBAL NEED

Permission to use by Scott Robertson

Permission to use by Scott Robertson

DHA content in Farmed and wild Atlantic Salmon (mg/100g)

Salmon DHA content decreasing with high prices of fish oil

HOW HAPPY WILL CONSUMERS BE WHEN THEY FIND OUT?

Genetically modified yeast or algae:

What is Alltech Algae SP1?

• 50 % fat

• 14% DHA

• 14% protein

• Schizochytrium limacinum

10.000 ton/year14 % DHA

How many factories

do we need in Japan?50.000 ton FO /yr10 % DHA5000 ton DHA

Algae factory:10.000 ton/yr14 % DHA1400 ton DHA

Oil EPA (%FA) DHA (%FA)

Herring 8.1 4.8

Mackerel 7.6 7.7

Anchovy 17 8.8

Menhaden 11 9.1

Pollock 12.6 9.7

Salmon farmed byproduct 7.7 9.8

Krill 17.4 11.4

Cod liver 11.2 12.6

Sardine (pacific) 16.9 12.9

3X

Impact of 15% Algae on Rainbow Trout

Treatment Average Weight of Trout (g)

Week 0

Week 5 Week 7 Week 10 Week 14

7% F.O./25% F.M. 6.0 20 29 52 97

15% algae/18% F.M.

6.0 21 30 53 111

Treatment Average Weight Gain of Trout (g)

Week 5

Week 7 Week 10 Week 14DHA

(mg/100g)

7% F.O./25% F.M. 14 22 45 91 1700

15% algae/18% F.M. 15 23 47 105 3000

Two diets (0, 15% Alltech SP1) for 14 weeks

F.O.= Fish Oil; F.M.= Fish Meal Filer, 2012

Impact of Alltech SP1 on Tilapia

Treatment Average Fish Weight (g) DHA

Week 0 Week 3 Week 8 Week 10 Week 13 (mg/100g)

No Algae 9.8 15.4 25.3 28.6 33.1 176c

0.2% 9.8 15.8 25.9 29.1 36.7 178c

0.4% 9.7 16.0 25.4 28.3 34.3 227b

0.8% 9.8 16.0 26.4 30.2 35.9 293a

P value 0.95 0.61 0.69 0.65 0.38 0.001

Four Diets (0, 0.2, 0.4, 0.8% Alltech SP1)

Adding 1 % SP1 Doubles the level of DHASignificant increase of feed cost

Cost per gram of DHA (Yen/g DHA)

1 % enriched Tilapia:2 $ per g DHA

Is it economical?

Reduce pre-termbirth risk

Generalimmunity

Braindevelopment

SupportsEye health

Maintain normal

triglyceride levels, heart rate and blood pressure

How much is Consumer willing to payFor DHA?

w3 versus DHA

OUR FUTURE IS BRIGHT!

Fish feed without FM and without FO for an economical cost:

Estimate: replace FM with SBM/CGM: - 20 %replace FO with algae: + 20 %

KOCHI-ALLTECH AquacultureResearch Alliance2013-2018Partners: Prof Fukada, Prof Ohshima, Prof. Satoh

COMPETITION: DHA Enriched

Eggs around the world

Eco eggs- Australia

DHA Omega 3- Dubai

Suguna DHA eggs- India

Chino valley ranchers-USA

Gegeda eggs, China

Omega-3 & DHA Enriched or Fortified Milk

around the world

USA

USA

USA

KoreaIreland

Spain

European DHA Products

Thank you.

Serge Corneillie & Renato Kitajima

Alltech