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APPLICATIONS OF

ENZYMES

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INDUSTRY ENZYME APPLICATIONS

1. Dairy Renin Manufacture of cheese

Lipases Cheese ripening and Icecream

manufacture.2. Food Proteases Biscuit and Pastry making

Amylases- and ,

invertase,glucose

isomerase

Production of glucose, maltose and

high fructose syrup.

Papain Tenderization of meat

Pectinase and

cellulase

Reduce viscosity and increase in

juice yield.

3. Detergent Proteases Removes stains and dirt

Amylases Removes stains and dirt

Cellulases Washing cotton fabrics

4. Baking -amylase Production of bread, buns and rolls.

Proteinase Biscuit manufacture

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5. Brewing and amylase and -

glucanase

Alcohol production

Amyloglucosidase and

proteinases

Production of low calorie beer and

remove cloudiness during storage.

6. Textile and amylase Desizing and binding agents on

threads of certain fabrics to prevent

damage during weaving

Glucoamylase desizing

Glucose isomerase Fructose syrup

7.. Molecular Biology Polymerases Polymerise DNA or RNA chain against

other DNA or RNA templates or

matrices

Restrictases Cuts double-stranded DNA and RNA

chains.

DNA ligases Link together DNA strands in places of

double strand breaks.

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8. Leather Trypsin Removes hairs from hides and skins to

increase softness

9. Pharmaceutical and

medical

Penicillin G/V acylase Production of semisynthetic pencillins.

Glucose isomerase Fructose syrup

Trypsin Dissolving blood clots

Pancreatic trypsin Treatment of inflammations.

amylases, lipasesand proteases

Digestive disorders

Asparaginase and

glutaminase

Leukemia

Ribonuclease Antiviral agent

Rhodonase Cyanide poisoning

-Lactamase Pencillin allergy

Streptokinase and

Urokinase

Treatment of the disorders of blood

circulation

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10. Medical diagnostics

Enzymes Normal Value Conditions in which enzyme levels are

altered

1. Heart diseases

CPK 4-60 IU/L AMI, Muscular dystrophy

SGOT 8-40 IU/L MI, Muscle diseases, liver diseases

LDH 60-250 IU/L AMI, Acute hepatitis, Leukemias,

Per.anaemia, muscle diseases.

2. Liver diseasesSGOT 8-40 IU/L MI, Muscle diseases, liver diseases

SGPT 5-35 IU/L Acute hepatitis, Jaundice

ALP 3-13 KA units Obstructive jaundice,Acute liver

diseases,Bone diseases, Rickets,Pagets

disease

3. GI Tract disorders

Serum Amylase 80-180 somogyi uints AP, Mumps, peptic ulcer, Intestinal

obstruction

Serum Lipase 9-20 IU AP, PU, Cirhosis, Pancreatic

carcinoma

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4. Muscle diseases

Aldolase 2-6 IU Muscular dystrophy, Acute MI, DM,

Leukaemia.

CPK 4-60 IU/L AMI, Muscular dystrophy

5. Bone diseases

ALP 3-13 IU/L Obstructive jaundice,Acute liver

diseases,Bone diseases, Rickets,Pagets

disease

6. Enzymes inmalignancies

ACP 0.6-3.1 KA units Gauchers disease,Pagets disease,

hyper parathyroidism, thrombocytosis,

prostatic carcinoma

LDH 60-250 IU/L AMI, Acute hepatitis, Leukeamias,Per.anaemia, muscle diseases.

Leucine amino

peptidase

15-56 IU Liver cell carcinoma, Cirrhosis and

viral hepatitis.

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11. Analytical Reagents

Glucose Oxidase Glucose in blood and body fluids

Uricase Serum Uric acid

Urease Urea in blood and body fluids