application of uv leds for turbid wasterwater disinfection

8/16/2019 Application of UV LEDs for Turbid Wasterwater Disinfection

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Appendix AExperimental data


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The log-reduction of E. coli in clean water at different exposure time was presented in this part. Pour plate technique has been applied to count the density of E. coli , and the agar for pour plate was Chromocult Coliform agar.

Table B.1 Log-reduction of E. coli in Clean Water at 15.37 & 36.62 s

Time for experiment Mar. 4th, 2014UV exposure time (s) 0 15.37 36.62

Density of E. coli ( 10 7 CFU/ mL) 5.3 1.04 0.0073log-reduction 0 -0.71 -2.86



Density of E. coli (× 107 CFU/ mL) 1.48 0.15 0.00013log-reduction 0 -0.99 -4.07



Density of E. coli (× 107 CFU/ mL) 1.48 0.23 errorlog-reduction 0 -1.16 error



Density of E. coli (× 107 CFU/ mL) 6 1.07 0.00069log-reduction 0 -0.75 -3.94

Table B.5 Log-reduction of E. coli in Clean Water at 24.31, 28.28 & 32.31 s

Time for experiment Mar. 11th, 2014

UV exposure time (s) 0 24.31 28.28 32.31Density of E. coli (× 107 CFU/ mL) 4.2 0.053 0.0096 0.019

log-reduction 0 -1.9 -2.64 -2.35

Table B.6 Log-reduction of E. coli in Clean Water at 24.28, 28.34 & 33.16 s

Time for experiment Mar. 12th, 2014UV exposure time (s) 0 24.28 28.34 33.16

Density of E. coli (× 107 CFU/ mL) 6.1 0.088 0.082 0.0046log-reduction 0 -1.84 -1.87 -3.12


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The log-reduction of E. coli in synthetic wastewater of 70 NTU were presented here andmontmorillonite was used to synthetize the turbid wastewater.

Table B.7 Log-reduction of E. coli in Synthetic Wastewater of 70 NTU (1 st trial)

Time for experiment Mar. 14th, 2014

UV exposure time (s) 0 75.16 125.16Density of E. coli (× 107 CFU/ mL) 5.5 0.00018 0.000002

log-reduction 0 -4.8 >7

Table B.8 Log-reduction of E. coli in Synthetic Wastewater of 70 NTU (2 nd trial)

Time for experiment Mar. 15th, 2014UV exposure time (s) 0 32.03 42.56 52.09 59.31 77.31

Density of E. coli (× 107 CFU/ mL) 3.9 0.047 0.067 0.018 0.0075 0.0035

log-reduction 0 -2.22 -2.07 -2.63 -3.01 -3.35

Table B.9 Log-reduction of E. coli in Synthetic Wastewater of 70 NTU (3 rd trial)

Time for experiment Mar. 17t , 2014UV exposure time (s) 0 15.50 25.25 60.03 70.91

Density of E. coli (× 107 CFU/ mL) 4.6 >3 2.82 0.2 0.045log-reduction 0


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The result of disinfection test with synthetic wastewater of 113 NTU were presented here.

Table B.12 Log-reduction of E. col i in Synthetic Wastewater of 113 NTU (1 st trial)

Time for experiment Mar. 23rd, 2014UV exposure time (s) 0 30 50.03 70.19 93.84 109.88

Density of E. col i (× 107 CFU/ mL) 1 0.96 0.65 0.53 0.015 0.0073log-reduction 0 -0.018 -0.19 -0.28 -1.84 -2.14

Table B.13 Log-reduction of E. col i in Synthetic Wastewater of 113 NTU (2 nd trial)


Density of E. coli (× 107 CFU/ mL) 0.92 0.028 0.0129 0.0078 0.00017 0.0002log-reduction 0 -1.52 -1.85 -2.07 -3.75 -3.66

The result of disinfection test with synthetic wastewater of 113 NTU were presented here.

Table B.14 Log-reduction of E. col i in Synthetic Wastewater of 27 NTU (1 st trial)


Density of E. col i (× 107 CFU/ mL) 2.9 1.16 0.81 0.19 0.016 0.0086log-reduction 0 -0.4 -0.55 -1.18 -2.25 -2.53

Table B.15 Log-reduction ofE. col i

in Synthetic Wastewater of 27 NTU (2nd

trial)


Density of E. coli (× 107 CFU/ mL) 4.4 0.073 0.036 0.01 0.00123 0.000125log-reduction 0 -1.78 -2.08 -2.64 -3.55 -4.55


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In this part, the result of disinfection test with real wastewater was presented, including theresult for total coliform and E. coli . The densities of both total coliform and E. coli werecounted by MPN method.

Table B.16 Log-reduction of total coliform in Real Wastewater of 86 NTU

Time for experiment April 10th, 2014Turbidity (NTU) 86exposure time (s) 0 31.65 55.4 72.41 90.81

total coliform (× 10 6 MPN/100 mL) 13 2.3 0.8 0.3 0.07log-reduction 0 -0.75 -1.21 -1.64 -2.27

E. coli detection failed.

Table B.17 Log-reduction of total coliform in Real Wastewater of 57 NTU

Time for experiment April, 11th, 2014Turbidity (NTU) 57exposure time (s) 0 32.78 53.31 76.29 91.34 111.87

total coliform (× 10 6 MPN/100 mL) 17 5 0.8 0.23 0.07 0.0011log-reduction 0 -0.53 -1.33 -1.87 -2.39 -4.19

E. coli detection failed.

Table B.18 Log-reduction of total coliform and E. col i in Real Wastewater of 130NTU

Time for experiment April 14th, 2014Turbidity (NTU) 130 NTUexposure time (s) 0 40.25 70.34 90.22 119.97 149.72

total coliform (× 10 6 MPN/100 mL) 13 3 0.17 0.035 0.022 0.013log-reduction 0 -0.64 -1.88 -2.57 -2.77 -3

E. coli (× 10 6 MPN/100 mL) 3 1.7 0.11 0.028 0.005 0.003log-reduction 0 -0.27 -1.44 -2.03 -2.78 -3

Table B.19 Log-reduction of total coliform and E. col i in Real Wastewater of 72 NTU

Time for experiment April 17th, 2014Turbidity (NTU) 72exposure time (s) 0 31.81 60.91 120.53 140.35

total coliform (× 10 6 MPN/100 mL) 5 1.3 0.09 0.009 0.0034log-reduction 0 -0.59 -1.74 -2.74 -3.17

E. coli (× 10 6 MPN/100 mL) 2.2 0.35 0.005 0.0021 0.0034log-reduction 0 -0.80 -2.64 -3.02 -2.81


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Appendix BExperimental set-up


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Figure A.1 Details of experimental set-up

application of uv leds for turbid wasterwater disinfection

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