appendixs1 genbank localitycampsicnemus eurynogaster complex. goodman et al. appendix s1:...

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES Multiple, Independent Colonizations of the Hawaiian Archipelago by the Family Dolichopodidae (Diptera) Appendix S1. Taxonomic sampling, GenBank accession numbers, partitioning and supplementary trees. TABLE S1A. TAXON SAMPLING AND GENBANK ACCESSION NUMBERS FOR EURYNOGASTER COMPLEX, CONCHOPUS, AND NON-HAWAIIAN ENDEMIC DOLICHOPODIDAE INCLUDED IN THIS STUDY.

Genus species Locality LabelName ND2 COI COII 12s 16s CAD EF1aA EF1aB

Eurynogaster complex

specimens

Adachia apicenigra HAWAII, Olaa Forest, Pole 44 Ad.apicenigra107 KX781491 KX781446 KX781540 KX781592 KX781644 KX781396 KX808417

Adachia hispida HAWAII, Olaa Forest, Puu Unit, 4300' Ad.hispida200978 KX781492 KX781447 KX781541 KX781593 KX781645 KX781397 KX808394

Adachia n.sp.nr.hispida

KAUAI, Kawaikoi Stream, 3500', 18 May 2007, sweeping over stream Ad.sp.nr.hispida205015 KX781493 KX781448 KX781542 KX781594 KX781646 KX781398 KX808395 KX808418

Arciellia dolichostoma KAUAI, Puu O Kila Road, 4080' Ar.dolichostoma205016 KX781494 KX781449 KX781568 KX781595 KX781647 KX781399 KX808396 KX808419

Arciellia dolichostoma KAUAI, Pihea Trail, 3600' Ar.dolichostoma205017 KX781495 KX781450 KX781569 KX781596 KX781648 KX781400 KX808397 KX808420

Arciellia dolichostoma MAUI, Haleakala NP, Kaupo Trail Ar.dolichostoma205359 KX781496 KX781451 KX781572 KX781597 KX781649 KX808421

Arciellia xanthopleura KAUAI, Kokee SP, Nualolo Tr. Ar.xanthopleura147 KX781570 KX781598 KX781650 KX781401 KX808422

Arciellia xanthopleura KAUAI, Pihea Trail, 3600' Ar.xanthopleura205018 KX781497 KX781452 KX781571 KX781599 KX781651 KX781402 KX808398 KX808423

Eurynogaster cilifemorata OAHU, Poamoho, 1.5 mi. E.cilifemorata94 KX781512 KX781561 KX781613 KX781664 KX781416 KX808436

Eurynogaster clavaticauda OAHU, Poamoho, stream E.clavaticauda95 KX781513 KX781466 KX781554 KX781614 KX781665 KX781417 KX808403 KX808437

Eurynogaster kauaiensis KAUAI, Pihea Trail E.kauaiensis131 KX781514 KX781566 KX781615 KX781666 KX781418 KX808438

Eurynogaster maculata OAHU, Poamoho Tr. E.maculata132 KX781515 KX781467 KX781556 KX781616 KX781667 KX781419 KX808439

Eurynogaster maculata MAUI, Paliku, crater wall, 6600' E.maculata205058_205380 KX781516 KX781468 KX781557 KX781617 KX781668 KX781420 KX808404 KX808440

Eurynogaster n.sp.A HAWAII, Hakalau E.n.sp.A133 KX781517 KX781549 KX781618 KX781669 KX781421 KX808441

Eurynogaster n.sp.B OAHU, Mt. Kaala E.n.sp.B105 KX781562 KX781621 KX781672 KX781424 KX808444

Eurynogaster n.sp.C OAHU, Poamoho Tr. E.n.sp.C128 KX781518 KX781469 KX781563 KX781619 KX781670 KX781422 KX808405 KX808442

Eurynogaster paludis MAUI, Pihea Trail, 3600' E.paludis205025 KX781519 KX781470 KX781567 KX781620 KX781671 KX781423 KX808406 KX808443

Eurynogaster sp.nr.cilifemorata OAHU, Mt. Kaala E.sp.nr.cilifemorata102 KX781560 KX781673

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES Multiple, Independent Colonizations of the Hawaiian Archipelago by the Family Dolichopodidae (Diptera)

Eurynogaster sp.nr.cilifemorata MAUI, Haiku Uka, Heed Trail, 4200' E.sp.nr.cilifemorata205049 KX781520 KX781471 KX781559 KX781622 KX781674 KX781425 KX808407 KX808445

Eurynogaster nr. cilifemorata

MAUI, East Maui Irrigation, Haiku Uka,Heed Trail E.sp.nr.cilifemorata83 KX781521 KX781472 KX781552 KX781623 KX781675 KX781426 KX808408 KX808446

Eurynogaster sp.nr.clavaticauda OAHU, Poamoho, stream E.sp.nr.clavaticauda93 KX781522 KX781473 KX781555 KX781624 KX781676 KX781427 KX808409

Eurynogaster sp.nr.hawaiiensis MAUI, Haiku Uka,Carson Trail, 4200' E.sp.nr.hawaiiensis205031 KX781523 KX781474 KX781564 KX781625 KX781677 KX781428 KX808410 KX808447

Eurynogaster sp.nr.hawaiiensis

MAUI, East Maui Irrigation, Haiku Uka, Carson Trail E.sp.nr.hawaiiensis80 KX781524 KX781475 KX781565 KX781626 KX781678 KX781429 KX808411 KX808448

Eurynogaster sp.nr.maculata HAWAII, Hakalau E.sp.nr.maculata115 KX781547 KX781627 KX781679

Eurynogaster sp.nr.maculata HAWAII, Kohalas, Puu O Uni NAR E.sp.nr.maculata126 KX781525 KX781476 KX781550 KX781628 KX781680 KX781430 KX808449

Eurynogaster sp.nr.maculata MAUI, Trail to Kawiki, 1 mi. from Paliku cabin E.sp.nr.maculata141 KX781526 KX781477 KX781558 KX781629 KX781681 KX781431 KX808450

Eurynogaster sp. HAWAII, Olaa Forest, Pole 44 E.sp112 KX781527 KX781478 KX781548 KX781630 KX781682 KX781432 KX808412

Eurynogaster sp. MAUI, Waikamoi Forest Preserve, Heed Trail E.sp135 KX781528 KX781479 KX781553 KX781631 KX781683 KX781433 KX808451

Eurynogaster variabilis HAWAII, Kau FR, Alahi Spring E.variabilis205226 KX781529 KX781480 KX781551 KX781684 KX781434 KX808452

Elmoia bullata

HAWAII, Kohala State Forest, trail to Waimanu Stream El.bullata205260 KX781530 KX781543 KX781632 KX781685 KX781435 KX808413 KX808453

Elmoia saxatilis OAHU, Mt. Kaala El.saxatilis106 KX781481 KX781546 KX781633 KX781686 KX781436 KX808454

Major minor OAHU, Mt. Kaala M.minor205097_111 KX781531 KX781482 KX781544 KX781634 KX781687 KX781437 KX808455

Sigmatineurum n.sp.D HAWAII, Kohala Mountains Si.n.sp.D205265 KX781532 KX781483 KX781545 KX781635 KX781688 KX781438 KX808456

Sweziella tergoprolixa MAUI, Haiku Uka, Heed Trail, 4200' Sw.tergoprolixa205048 KX781533 KX781484 KX781578 KX781636 KX781689 KX781439 KX808414 KX808457

Uropachys clavastyla KAUAI, Kokee SP, Nualolo Tr. U.clavastyla130 KX781486 KX781575 KX781639 KX781692 KX781441 KX808459

Uropachys fusticercus KAUAI, Na Pali-Kona FR Pihea Trail U.fusticercus205027 KX781536 KX781487 KX781573 KX781640 KX781693 KX781442 KX808416 KX808460

Uropachys fusticercus KAUAI, Na Pali-Kona FR Pihea Trail U.fusticercus61 KX781537 KX781488 KX781574 KX781641 KX781694 KX781443 KX808461

Uropachys palustricola KAUAI, Alakai Swamp GB27A U.palustricola127 KX781538 KX781489 KX781576 KX781642 KX781695 KX781444 KX808462

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES Multiple, Independent Colonizations of the Hawaiian Archipelago by the Family Dolichopodidae (Diptera)

Uropachys palustricola KAUAI, Pihea Trail U.palustricola134 KX781539 KX781490 KX781577 KX781643 KX781696 KX781445 KX808463

Other Hawaiian endemic Dolichopodidae specimens

Conchopus ciliatus

HAWAII, Whittington Beach Park, South Coast, Kau Conchopus.ciliatus96 KX781504 KX781459 KX781590 KX781656 KX781408 KX808428

Conchopus pacificus

HAWAII, Whittington Beach Park, South Coast, Kau Conchopus.pacificus122 KX781505 KX781460 KX781591 KX781606 KX781657 KX781409 KX808429

Conchopus acrostichalis OAHU, Haleiwa Beach Conchopus.acrostichalis92 KX781503 KX781458 KX781589 KX781605 KX781655 KX781407 KX808427

Thinophilus hardyi

HAWAII, Whittington Beach Park, South Coast, Kau Th.hardyi205118 KX781535 KX781588 KX781638 KX781691

Non-Hawaiian endemic

Dolichopodidae specimens

Chrysotus longipalpis OAHU, Manoa Cliff Trail, 1800' Ch.longipalpis205060 KX781498 KX781453 KX781579 KX781600 KX781652 KX781403 KX808424

Chrysotus longipalpis

OAHU, Honolulu Watershed FR, Manoa Cliffs Trail Ch.longipalpis205379 KX781499 KX781454 KX781581 KX781601 KX781653 KX781404 KX808425

Chrysotus sp MARQUESAS, HIVA OA Ch.sp173 KX781502 KX781457 KX781604

Chrysotus sp nr.longipalpis MAUI, Kahanaiki Gulch, 2100' Ch.sp.nr.longipalpis205030 KX781500 KX781455 KX781602 KX781405 KX808426

Chrysotus sp.nr.longipalpis MAUI, Puu Kukui, Kahanaiki Gulch Ch.sp.nr.longipalpis85 KX781501 KX781456 KX781580 KX781603 KX781654 KX781406

Condylostylus sp. OAHU, Maunaloa Beach Rocks Condylostylus.sp164 KX781506 KX781461 KX781607 KX781658 KX781410 KX808430

Dolichopus exsul OAHU, Poamoho D.exsul169 KX781507 KX781585 KX781608 KX781659 KX781411 KX808431

Dolichopus exsul KAUAI, Pihea Trail, 3500' D.exsul205024 KX781509 KX781462 KX781583 KX781609 KX781660 KX781412 KX808399 KX808432

Dolichopus exsul MAUI, Puu Kukui Trail, 2900-3700' D.exsul205034 KX781508 KX781463 KX781582 KX781610 KX781661 KX781413 KX808400 KX808433

Dolichopus exsul HAWAII, Kau FR, Alahi Spring D.exsul205232 KX781510 KX781464 KX781584 KX781611 KX781662 KX781414 KX808401 KX808434

Dolichopus exsul HAWAII, Kohala Mountains D.exsul205282 KX781511 KX781465 KX781586 KX781612 KX781663 KX781415 KX808402 KX808435

Tachytrechus angustipennis KAUAI, Kumuwela Trail, 3500' T.angustipennis205026 KX781534 KX781485 KX781587 KX781637 KX781690 KX781440 KX808415 KX808458

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES TABLE S1B. SUMMARY OF DATA PARTITIONS AND NUCLEOTIDE MODELS DETERMINED FOR EACH PARTITION, SELECTED USING BIC IN PARTITIONFINDER (LANFEAR ET AL., 2012).

Program (# partitions)

Figure Data partitions: nucleotide models selected by PartitionFinder

Dataset A Dolichopodidae Mr Bayes/ RAxML(11)

Figures 2, S1a & S1b in Appendix 1

Mr Bayes: COI_3, COII_3, ND2_3: GTR+I+G COI_1, COII_1: GTR+I+G EF1_2, COI_2, COII_2: GTR+I+G ND2_1: GTR+G ND2_2: GTR+G 12S, 16S: GTR+I+G CAD_3: HKY+I+G CAD_1, CAD_2: K80+I+G CADintron: GTR+I+G EF1_3: HKY+G EF1_1: F81+I+G RAxML: Each partition was run using the GTR+GAMMA model

Dataset B (Eurynogaster complex): Mr Bayes/ RAxML(11)

Figure 1 & Figure S1c-l in Appendix 1

Mr Bayes: CAD_3: HKY+I CAD_1: GTR+G CAD_2, EF1A_2, ND_2: HKY+I+G CADintron, EF1Aintron1, EFAintron2: HKY+I EF1A_3, EF1B_3: HKY+G EF1A_1, EF1B_1: F81+I COI_2, COII_2, EF1B_2: F81+I+G EF1Bintron: F81 12S, 16S, ND2_1: GTR+I+G COI_3, COII_3, ND2_3: GTR+I+G COI_1, COII_1: GTR+G RAxML: Each partition was run using the GTR+GAMMA model

Dataset B (Eurynogaster complex): BEAST: Island calibrations (10)

Figures 1 & S2b in Appendix 2

CAD_3: HKY CAD_1, EF1A_1, EF1B_1: TrN+I CAD_2, EF1A_2, ND_2: HKY+I+G CADintron, EF1Aintron1, EF1Aintron2: HKY+I EF1A_3, EF1B_3: HKY+G COI_2, COII_2, EF1B_2: HKY+I+G EF1Bintron: HKY 12S, 16S, ND2_1: GTR+I+G COI_3, COII_3, ND2_3: GTR+I+G COI_1, COII_1: GTR+G

Dataset B (Eurynogaster complex): BEAST: Divergence rates (11)

Figures S2c & S2d in Appendix 2

COI: GTR+I+G CAD_3: HKY+I CAD_1, EF1A_2, EF1B_1: TrN+I CAD_2, EF1A_2, ND2_2: HKY+I+G CADintron, EF1Aintron1, EF1Aintron2: HKY+I EF1A_3, EF1B_3: HKY+G COII_2, EF1B_2: HKY+I EF1Bintron: HKY 12S, 16S, ND2_1: GTR+I+G COII_3, ND2_3: GTR+I+G COII_1: TrN+G

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES SUPPLEMENTARY TREES: FIGURE S1A. COMBINED DOLICHOPODIDAE ANALYSIS (DATA SET A). Majority rule consensus tree summarizing Bayesian analysis, with the Eurynogaster complex and Campsicnemus collapsed. Bayesian posterior probabilities (Mr. Bayes) and bootstrap supports from the maximum likelihood analysis (RAxML) are displayed as boxes.

0.3

Teuchoph.tiomanensisGB

Synt.pallipesGB

Ch.longipalpis205379

Ch.sp.nr.longipalpis85

Chaetogon.chaeturumGB


Chaetogon.sp2GB

Y.callida205086Symp.taenicornis205085

Synt.denticulatusGB

Teuchoph.nigricostaGBTeuchoph.stenostigmaGB


Synt.pumilumGB

Teuchoph.calcarGB

Synt.flexibileGB

D.exsul205024

Teuchoph.monacanthusGB

Condylostylus.sp164Conchopus.ciliatus96

D.exsul205232

Th.hardyi205118

Scotiom.spGB

AnepsiomyiaflaviventrisGB

Teuchoph.ornatulusGB

D.exsul169

Symp.turbidus195

Ch.sp173

D.exsul205282

Symp.kingstoni205083

Symp.sp1SNK2009GB

Conchopus.acrostichalis92

D.exsul205034

Scotiom.singaporensisGB

T.angustipennis205026

Synt.zelleriGB

Empis_sp

Conchopus.pacificus122

Chersodromia_spDrapetis_sp

Symp.desoutteriGB1

Teuchoph.enormisGB

Empis_producta

Synt.flexibile104

1

1

11

1

11

1

ML Bootstrap

Posterior Probability95-100%75-95%50-75%

Campsicnemus

Eurynogaster Complex

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1B. MAXIMUM LIKELIHOOD ANALYSIS OF THE DOLICHOPODIDAE (DATA SET A).

0.2

Hercostom.indonesianusGB

Ch.sp173


Teuchoph.tiomanensisGB

Symp.kingstoni205083


Symp.desoutteriGB1

D.exsul205232

Chaetogon.chaeturumGB


Symp.taenicornis205085

Condylostylus.sp164

Teuchoph.nigricostaGB

Empis_producta

Chaetogon.sp2GB

C.rhenocrenus201853

Symp.turbidus195

D.exsul205034

Y.callida205086Teuchoph.monacanthusGB


Empis_sp

D.exsul169

Teuchoph.calcarGB

Drapetis_sp

Synt.pumilumGB

Conchopus.ciliatus96

Scotiom.spGB

D.exsul205024

Synt.flexibile104

Scotiom.singaporensisGB

Teuchoph.enormisGB

Teuchoph.stenostigmaGB

Synt.denticulatusGB

Synt.flexibileGB

Chersodromia_sp

AnepsiomyiaflaviventrisGB

D.exsul205282

Synt.zelleriGB

Th.hardyi205118

Synt.pallipesGB


Symp.sp1SNK2009GB



Teuchoph.ornatulusGB

11

100

20

100

32

26

25

18

85

58

41

69

11

57

5

100

7

5

99

93

100

50

76

95

100

100

16

100

100

98

54

35

99

27

100

100

3437

99

100

36

97

58

71

77

40100

53

Eurynogaster complex

Campsicnemus

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1C. Majority rule consensus tree summarizing Bayesian analysis of the Eurynogaster complex. Bayesian posterior probabilities (Mr. Bayes) and bootstrap supports from the maximum likelihood analysis (RAxML) are displayed as boxes. Islands that each specimen was collected from are shown next to each tip.

0.94

0.97

Adachia

A. sp. nr. hispida 201015

A. apicenigra 107

A. hispida 200978

E. bullata 205260

S. n. sp. D 205265

E. saxatilis 106

M. minor 205097_111

Sigmatineurum

Major

Elmoia

S. tergoprolixa 205048 SweziellaA. dolichostoma 205016

A. dolichostoma 205017

A. dolichostoma 205359

A. xanthopleura 147

A. xanthopleura 205018

Arciellia

U. palustricola 127

U. palustricola 134

U. clavastyla 130

U. fusticercus 205027

U. fusticercus 61

E. kauaiensis 131

Uropachys

E. paludis 205025

E. sp. nr. hawaiiensis 205031

E. sp. nr. hawaiiensis 80

E. sp. nr. cilifemorata 102


E. n. sp. C 128

E. cilifemorata 94

E. n. sp. B 105

E. maculata 132

E. clavaticauda 95

E. sp. nr. clavaticauda 93


E. sp. 135

E. sp. nr. maculata 141

E. maculata 205058_205380


E. variabilis 205226

E. sp. 112

E. n. sp. A 133


Eurynogaster

Condylostylus

Chrysotus

Hercostomus

Dolichopus

Conchopus

Tachytrecus

Eurynogaster Com

plex

0.2

Thinophilus

Posterior Probability

ML Bootstrap

95-100%75-95%

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1D. COMBINED MAXIMUM LIKELIHOOD ANALYSIS OF ALL GENES (DATA SET B).

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES SUPPLEMENTARY TREES: INDIVIDUAL MAXIMUM LIKELIHOOD GENE TREES: FIGURE S1E. COI

0.07

Ad.sp.nr.hispida205015

E.sp.nr.maculata126

E.sp.nr.cilifemorata83

Conchopus.pacificus122Conchopus.acrostichalis92


U.clavastyla130

D.exsul205034

M.minor205097_111

Ad.hispida200978

U.fusticercus61

E.sp135

Ar.dolichostoma205017

E.sp.nr.hawaiiensis205031

E.sp.nr.clavaticauda93

D.exsul205282

Condylostylus.sp164D.exsul205232

E.clavaticauda95

U.palustricola127

U.fusticercus205027

E.sp.nr.maculata141

Ch.sp173

E.variabilis205226



E.cilifemorata94



Sw.tergoprolixa205048

E.maculata205058_205380

P.metallicus78

Ad.apicenigra107

El.saxatilis106

E.paludis205025

D.exsul205024


Ar.xanthopleura205018




E.maculata132

E.n.sp.3.128

E.sp112

Si.n.sp.1.205265


U.palustricola134


97

97

100

100

100

99

100

55

95

59

100

96

61

40

97

100100

100

90

8798

100

69

100

64

100

85

99

29

100

37

69

41

100

91

37

26

82

58

22

60

68

100

97

18

77

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1F. COII

0.06

E.maculata205058_205380


E.maculata132


D.exsul205232

El.saxatilis106


E.sp.nr.maculata126

Ad.hispida200978


U.clavastyla130


P.metallicus78

Si.n.sp.1.205265


E.sp.2.nr.maculata105

D.exsul205282



E.sp112

D.exsul205034

E.cilifemorata94

E.kauaiensis131



U.fusticercus61


E.sp135


E.sp.nr.maculata115

U.fusticercus205027


Th.hardyi205118


Ad.apicenigra107


E.sp.nr.maculata141

Ar.xanthopleura147



E.n.sp.3.128


E.variabilis205226

E.n.sp.1.133

U.palustricola127

D.exsul205024

E.paludis205025

U.palustricola134


D.exsul169

M.minor205097_111

El.bullata205260

E.clavaticauda95

37

71

62

70

9973

97

85

96

100

31

20

23

55

66

31

99

10097

100

100

37

87

100

100

100

100

97

54

98

100

7786

9281

50

67

58

30

95100

11

95

43

66

6

100

37

100

81

98

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1G. ND2

0.2

U.palustricola134


E.variabilis205226E.sp.nr.maculata126


D.exsul205034

E.sp135

Si.n.sp.1.205265

E.sp.nr.clavaticauda93E.clavaticauda95


Condylostylus.sp164


Ad.hispida200978

Ar.dolichostoma205359Ar.xanthopleura205018

U.fusticercus61


E.n.sp.3.128


U.fusticercus205027

E.cilifemorata94

Ch.sp173


M.minor205097_111

D.exsul169

U.palustricola127


E.maculata132


E.paludis205025

E.sp.nr.maculata141

Ad.apicenigra107



El.bullata205260


D.exsul205282

E.sp112

Conchopus.acrostichalis92Conchopus.ciliatus96


E.n.sp.1.133


D.exsul205232

E.kauaiensis131

E.maculata205058_205380

Th.hardyi205118

D.exsul205024

99

77

77

85

16

100

77

75

51

74

96

100

55

81

92

86

78

100

100

94

98

100

94

100

100

67

100

41

98

100

99

67

82

22

66

100

99

96

26

91

99

23

32

100

52

60

62

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1H. 12S

0.06

E.sp112


M.minor205097_111

D.exsul205024

E.n.sp.1.133

P.metallicus78

E.sp.nr.maculata115

U.clavastyla130

Ad.hispida200978



Th.hardyi205118

E.sp.nr.maculata141

D.exsul169

U.fusticercus61



E.n.sp.3.128

U.palustricola134


D.exsul205282






E.sp135

E.paludis205025

Ch.sp173



E.sp.nr.maculata126


D.exsul205232

Si.n.sp.1.205265


Condylostylus.sp164E.clavaticauda95

E.kauaiensis131


E.cilifemorata94

U.palustricola127


Ad.apicenigra107

D.exsul205034


El.saxatilis106

U.fusticercus205027



El.bullata205260

E.maculata205058_205380

E.maculata132

Ar.xanthopleura147

99

95

70

94

62

69

12

100

37

11

89

93

31

86

74

100

74

97

19

73

94

21

100 56

28

91

100

94

19

10099

97

99

55

100

25

100

16

52

23

75

64

73

43

92

68

18

42

50

94

96

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1I. 16S

0.04

U.fusticercus205027


Si.n.sp.1.205265

D.exsul169

Ad.apicenigra107



E.sp112



E.sp.nr.maculata126


El.saxatilis106

E.maculata205058_205380

El.bullata205260

E.sp.nr.maculata141

E.sp135

U.palustricola134

D.exsul205034


D.exsul205024


E.cilifemorata94

E.sp.nr.maculata115




E.maculata132




Condylostylus.sp164

E.kauaiensis131

E.n.sp.1.133


E.variabilis205226


D.exsul205282

E.n.sp.3.128

E.paludis205025E.sp.2.nr.maculata105

Ch.longipalpis205379Ch.longipalpis205060


D.exsul205232

Ad.hispida200978

U.fusticercus61

P.metallicus78


U.clavastyla130

E.clavaticauda95

Th.hardyi205118

Ar.xanthopleura147

M.minor205097_111

U.palustricola127

96

13

100

23

10

9

14

3999

57

99

21

66

100

96

64

24

28

57

8 100

93

55

14

72

0

50

19

25

81

30

34

100

100

36

33

41

100

77

98

45

77

2

100

100

41

11

99

88

95

97

99

100

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1J. CAD

0.2

Condylostylus.sp164

Ad.hispida200978

E.sp.nr.maculata141

D.exsul205034


E.sp135

Ar.xanthopleura147



Si.n.sp.1.205265

M.minor205097_111


E.kauaiensis131

El.saxatilis106

E.paludis205025

Ad.apicenigra107

U.fusticercus205027



Ar.dolichostoma205016Ar.dolichostoma205017

E.n.sp.3.128

U.palustricola127


D.exsul205232

P.metallicus78


U.clavastyla130



E.n.sp.1.133

D.exsul205024


D.exsul169




E.maculata205058_205380




E.maculata132

E.sp.nr.maculata126

E.cilifemorata94

E.clavaticauda95

E.sp112

U.palustricola134

El.bullata205260

E.variabilis205226

U.fusticercus61

D.exsul205282

80

38

100

95

59

56

99

100

87

100

99

70

54

96

65

100

98

99

99

100

100

29

90

100

93

100

92

98

60

100

100

100

90

70

72

97

62

100

100

24

100

93

92

72

100

81

99

70

88

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1K. EF1AA

0.03

D.exsul205282

D.exsul205034

El.bullata205260


Ad.hispida200978

U.fusticercus205027

D.exsul205024







E.maculata205058_205380

D.exsul205232


E.sp112

E.n.sp.3.128

E.paludis205025


E.clavaticauda95



100

100

59

89100

31

43

99

100

100

85

10099

33

33

100

100

92

60

97

GOODMAN ET AL. APPENDIX S1: GENBANK ACCESSION NUMBERS, PARTITIONING AND SUPPLEMENTARY TREES: REPEATED INDEPENDENT RADIATIONS IN HAWAII BY LONG-LEGGED FLIES FIGURE S1L. EF1AB

0.04

D.exsul205232

Ar.xanthopleura147

E.variabilis205226



E.maculata205058_205380




E.n.sp.3.128

Si.n.sp.1.205265

E.paludis205025E.sp.nr.cilifemorata205049


E.sp135

E.sp.nr.maculata126

D.exsul205282



U.palustricola134

D.exsul205024

M.minor205097_111


El.bullata205260


U.fusticercus61

E.cilifemorata94


P.metallicus78



E.maculata132


D.exsul205034

E.sp.nr.maculata141

El.saxatilis106

E.kauaiensis131

Condylostylus.sp164

E.n.sp.1.133

E.clavaticauda95

U.palustricola127


D.exsul169

Ad.apicenigra107


U.clavastyla130

U.fusticercus205027


100

97

36

16

100

83

50

71

99

100

95

41

77

100

100

93

79

96

97

99

100

74

67

7

100

91

87

97

99

49

69

59

100

66

80

90

99

9189

99

63

87

100

99

83

78

appendixs1 genbank localitycampsicnemus eurynogaster complex. goodman et al. appendix s1:...

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