Quintessential Skincare of Today [PART I]

Et-VCTM

Holistic Approach to Skin Homeostasis & Defense

MultifunctionalActive

“Hydration Boost”

Corum in a Nutshell

Organic Chemistry

Biochemistry & Biotech

Application Process EngineeringQuality Control &

Assurance

With full dedication & prudence,

Corum inspired through knowledge to discover new efficacy and the best

quality ingredients to you!

Cosmetics and Personal Care Ingredients

Active Pharmaceutical Ingredients

Supported by our expertise in innovation R&D, formulation, scientific research, supply chain, production,

sourcing, regulatory and sales & marketing excellence, we manufacture a wide range of innovative ingredients

and specialty chemicals for the personal care and pharmaceutical industries.

COVID-19: What is happening? What to expect?Lives come first, but livelihoods also matter

Global BPC industry weak in Q1 with widespread store closures

Strong hit on makeup category

Stay-home economy booming

Rising stars:

IMMEDIATE & SUSTAINABLE

Clean beauty under transformation

Formulation efficacy Ingredient safety Shelf-stability

“DIY” and self care beauty

Growth for nail care & men’s grooming

“Touchless” beauty product

Sticks & sprays

Coronavirus Beauty EssentialsWhen masks have become a necessary accessory

01. Provide Extra Hydration to Mitigate Dry Condition and Prevent Skin Irritation- Use facial and hand cream rich in moisturizer- Choose non-irritating oil control and acne care treatment- Use lip balm with nourishing ingredients

02. Boost Skin Natural Defense- Feed skin with probiotic, vitamin and adaptogen

03. Sunscreen is Still a Must- Back to office and walking under the sun

04. Focus on Eyes , and Don’t Forget the Brow

05. Use Waterproof Foundation to Avoid Staining the Mask

◼ Less is more – achieving more with less

◼ Amp up skin natural defense

◼ Top demands: Glowing, purifying, disinfectant/antiseptic/antibacterial

Against the COVID-19The world is adapting and adopting the new norm.

Vitamin CPowerful Anti-Oxidant for Multiple Skincare Benefits

Can lessen redness and sunburn if applied before (or after) sun exposure

Neutralizes free radical damage

Helps moisturize and increase skin hydration

Stimulates collagen production

Prevents premature skin aging

Protects against damaging UVA and UVB rays

Reverses the appearance of photoaging

Decreases fine lines and wrinkles

Brightens the skin for more even tone

Fades brown spots

Anti-inflammatory

But!Pure vitamin C is unstable and sensitive to oxidation…

Ascorbic Acid

AscorbylPalmitate

MAP

AA2G

3-O-Ethyl Ascorbic

Acid

• Oil soluble

• Difficult to penetrate the epidermis

• Do NOT boost collagen

• Natural and Potent

• Easily oxidized in aqueous solution, vulnerable to heat, air and light

• Boost collagen

• Difficult to penetrate epidermis

• Higher molecular weight

• Hydrolyzed by α-glucosidasebefore exerting its function

• Lesser activity on tyrosinaseinhibition

• Most effective in boosting collagen

• Potent on skin lightening and brightening

• Easy to penetrate epidermis

Stabilized Vitamin CAscorbic Acid Derivative

Et-VCTM

3-O-Ethyl Ascorbic Acid

◼ Prevent photoaging

◼ Excellent anti-oxidation properties

◼ Inhibit melanogenesis

◼ Even skin tone and reduce dark spots

◼ Scavenge free radicals

◼ Anti-inflammaging

◼ Anti-blue light and anti-pollution

◼ Sun protection

◼ Boost hyaluronic acid synthesis

◼ Increase skin hydration and improves barrier function

◼ Excellent stability

Benefits Structure

CAS No. 86404-04-8MW: 204.18 g/mol

2 3

White crystalline powder

99% min

50%

3.5-5.0

Water soluble

Appearance

Purity (HPLC)

Active

pH value

Solubility

Properties

Skin with high hyaluronic acid content

Hyaluronic acid

Skin with low hyaluronic acid content

Hyaluronidase

Hyaluronic acid is naturally occurring polysaccharide in skin’s connective tissue. It binds up to 1000 times its volume in water to help skin retain moisture and suppleness. The amount of HA diminishes with age.

Hyaluronidases is the enzyme that catalyzes the degradation of hyaluronic acid into small HA fragments. Increasing the activityof hyaluronidase, resulting in lossof skin moisture and elasticity.

Et-VCTM keeps skin softer, smoother, supple and hydrated.

Et-VCT`M

Et-VCTM

Restore Skin HomeostasisNatural HA Booster

100107.2

148.8

182.7

0

20

40

60

80

100

120

140

160

180

200

Ctrl 0.001 0.01 0.1

Hya

luro

nic

aci

d s

ynth

esis

[%

]

Stimulation of Hyaluronic Acid Synthesis

Et-VCTM can significantly boost HA synthesis by up to 182.7%

Cell line: Hs68 (human fibroblast)

0.0 3.0

8.3

17.4

37.9

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

0EtVC (%)

w/hyaluronidase

0.63 1 2.5 5.0

Inh

ibit

ion

rat

e (%

)

Et-VC w/ hyaluronidase (%)

Inhibition on Hyaluronidase Activity

***

***

***

%

Efficacy TestStimulation of HA

82.7% increasing

Et-VC (%)

Et-VCTM can significantly inhibit hyaluronidase activity by 37.9%

*p<0.05, **<p<0.01, ***p<0.001Test: ELISA, Optical Density TestCorum R&D In-House Test May 2016

BLMHBleomycin Hydrolase

CAPN1Calpain1

Ep

ider

mis

Filaggrin

NMF

Filaggrin is a filament aggregating protein which is essential for regulating skin homeostasis and imparts skin barrier function. Filaggrin contributes to skin hydration through generation of free amino acids.

CAPN1 (Calpain 1) and BLMH (Bleomycin hydrolase) are filaggrin processing enzymes responsible for cleaving small filaggrin fragments

into amino acids for NMF formation.

NMF (natural moisturizing factor) maintains the adequate hydration of stratum corneum to impart barrier function which protects skin against harmful microorganisms and mechanical stress and activate hydrolytic enzyme necessary in desquamation. NMF keeps the skin supple and soft.

Keep Skin Hydrated

Et-VCTM

Stratum Corneum Layer

Stratum Granulosum Layer

Restore Skin HomeostasisNMF Formation

Protein level(ELISA)

Gene level(qPCR)

*

115.6

100.0

*

118.3

*

134.5

0.0

20.0

40.0

60.0

80.0

100.0

120.0

140.0

160.0

Basal CtrlBlue light

pre-treatment0.5% EtVC

post-treatment

Expression of Filaggrin

0.5% Et-VCTM

Blue light

Fila

gg

rin

(% o

f co

ntr

ol)

*

117.0 100.0

*

172.4 *

147.6

100.0

*

264.5

0.0

50.0

100.0

150.0

200.0

250.0

300.0

basal ctrlBue light

0.5% EtVC

Gene Expression of CALPN1 and BLMH

BLMH CAPN1

Basal Ctrl 0.5% Et-VCTM

Blue light

Rel

ativ

e m

RN

A e

xpre

ssio

n (

%)

*p<0.05, **<p<0.01, ***p<0.001

Efficacy TestFilaggrin and its Processing Enzymes (CAPN1 and BLMH)

Et-VCTM can significantly stimulate Filaggrin level by up to 134.5% and upregulate BLMH

and CAPN1 172.4% and 264.5%, respectively. Cell Line: HaCaTCorum R&D In-House Test, April 2020

iNOS COX-2

NO PGE-2

Inflammatory cytokines(IL-1α, IL-6, IL-8…)

MMPs

Collagen degradation

Premature aging/

wrinkle formation

UVAEpidermis

Dermis

DNA damage

ROSUVB

Inflammation

Et-VCTM

Defense Against UV

ROS: Reactive Oxygen Species IL: InterleukinMMP-1: Matrix Metalloproteinases-1COX-2: Cyclooxygenase-2PGE-2: Prostaglandin E2

iNOS: Inducible Nitric Oxide SynthaseNO: Nitric Oxide

44.9% 87.6%

84%

46%

27% IL-6 95%

IL-8 47%

IL-1α 30%

Et-VCTM shows remarkable ability to fight against photoaging. Test: DCFH-DA, ELISACell Line: NHEK, Raw 264.7Corum R&D In-House Test, February 2013

iNOS COX-2

NO PGE-2

Inflammatory cytokines(IL-1α, IL-6, IL-8…)

MMPs

InflammationCollagen

degradation

Premature aging/

wrinkle formation

Epidermis

Dermis

DNA damage

ROS

Urban Dust (SRM1649b)

Diesel particulate matter (SRM1650b)Et-VCTM

Defense Against Pollution

77% 61%

IL-8 56%

MMP-1 48%

IL-8 50%

MMP-1 67%

PGE-2 51%

DNA

ProteinIL-8 47%

MMP-1 75%

Protein

Et-VCTM effectively protects against pollution damage.Test: DCFH-DA, ELISA, qPCR Cell Line: HaCaT, NHEKCorum R&D In-House Test, July 2016

Et-VCTM

Defense Against Blue Light

Test: ELISA, qPCRCell Line: NHDFCorum R&D In-House Test, September 2017

IL-6 60%

IL-8 34%

InflammationCollagen reduction

Premature aging/

wrinkle formation

Epidermis

Dermis

Inflammatory cytokines

(IL-6, IL-8)

MMPs

TGF-b COX-2

Procollagen

Production

ROS

PGE-2

77%

HO-1DNA

Protein

IL-6 40%

IL-8 28%

32%

Col1A1 60%

TGF-b1 50%

Basal Ctrl. 0.5% Et-VC

Blue light irradiation

Lo

t1L

ot2

COL1A : GreenDAPI(nuclear): Blue

53%

Study Conditions:

◼ Method : Immunofluorescence

◼ Cell : NHDF

◼Time: pretreat Et-VC 1 day

Col1A1

The COL1A1 provides instructions for

making part of a large molecule called

type I collagen

Et-VCTM takes the protection to the next level to shield against blue light.

◼ Stimulator: Blue light

◼ Test items: Et-VC™

Efficacy TestIn-vivo Sun Protection Study

Purpose:

To evaluate the performance of

the sunscreen products with and

without Et-VCTM.

Study Conditions:

◼ Number of volunteers: 20

◼ Sex: Male or female

◼ Age: 18 or older

◼ Test Site:

The infrascapular area of back

◼ Test Material:

Sunscreen product with or without

Et-VC TM (3% )

Volunteer 1 Volunteer 2

by AMA Laboratories, Inc (USA)

With 3% Et-VCTM

Without Et-VCTM

Baseline Irradiation

Baseline Irradiation

Baseline Irradiation

Baseline Irradiation

Baseline Irradiation

Baseline Irradiation

L* 100% Insulta* 100% Insult

L* 68.4% Insulta* 91.6% Insult

L* 44.3% Insulta* 63.6% Insult

Efficacy TestIn-vivo Skin Brightening Study

Study Conditions:

◼ Number of volunteers: 20 healthy Asian -Female skin type III

◼ Age: 25-40 years old

◼ Test method: Chromametry (CR-300)

◼ Test Material: 2% Et-VCTM cream

32

33

34

35

T 0 T +28 Days

Me

an

Ind

ivid

ua

l T

yp

olo

gic

al A

ng

le

+4.0%

By Spincontrol, Asia

Volunteer 1 Volunteer 1

Volunteer2 Volunteer2

Efficacy TestIn-vivo Anti-Wrinkle Study

-7.8

-10

-4.9

-1.2

-19.6

-11.8

-6

-2.2

-20

-15

-10

-5

0

Total wrinklearea(mm2)

Total Length(mm)

Mean Length(mm)

Mean depth(mm)

Day 28Day 56

Va

ria

tio

ns

(%)

Baseline Day 28 Day 56

Baseline Day 28 Day 56

Vo

lun

teer 1V

olu

nteer 2

Study Conditions:

◼ Number of volunteers: 20

◼ Sex: Female

◼ Age: 30-55 year-old

◼ Test Material: 4% Et-VC TM cream

◼ Method: Quantirides system

By Spincontrol, Asia

Penetration TestEt-VCTM vs. AA2G

EPISKIN® Model:

◼ In-vitro reconstructed human epidermis similar to the in-vivo human epidermis.

◼Applications: Percutaneous absorption 0

5

10

15

20

25

30

35

40

0 1 2 3 4 5 6 7 8Time (hour)

Corum9515

AA2G

Cu

mu

late

d p

erm

ea

ted

am

ou

nt

(μg‧c

m-2

)

Et-VCTM

2.4 times better in penetration

0

2

4

6

8

10

12

14

0 1 2 3 4 5 6 7 8Time (hour)

Corum9515AA2G

Cu

mu

late

d p

erm

eate

d a

mo

un

t(μ

g‧c

m-2

)

5 times better in penetration

Et-VCTM

2 % Solution

2 % Cream

Stability TestEt-VCTM vs. Whitening Agents

Sample: 2 % solution

96.890.9

85.1

41.6

0

20

40

60

80

100

Corum9515

α-Arbutin AA2G Vit.C

Tra

nsm

itta

nce

(%

T)

Et-VCTM

◼ Under sunlight without buffer ◼ At 45 ̊C without buffer

FormulationHigh Concentration Vitamin C Serum

Anti-Aging

✓ Prevent photoaging

✓ Anti-inflammation

✓ Protect DNA

✓ Scavenge free radicals

Skin Whitening

✓ Inhibit melanogenesis

✓ Improve skin radiance

✓ Reduce dark spots and even the skin tone

UV, Blue Light and Pollution Protection

✓ Sun protection

✓ Protect the skin under blue light & pollution damage

Hydration Booster

✓ Natural hyaluronic acid booster

✓ Restore skin hydration & homeostasis

Et-VCTM

3-O-Ethyl Ascorbic Acid

*Regulatory status:Europe REACH compliantChina approvedHalal approved

Stable, multifunctional

Quintessential Skincare of Today [PART I]

Et-VCTM

Holistic Approach to Skin Homeostasis & Defense

MultifunctionalActive

“Hydration Boost”

Q&A

Email: sales@corum.com.tw