antinutritional factors proteins detoxification (1)
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Antinutritional factorsTRANSCRIPT
Antinutritional factors in Oil Meals and its effects
Sr.
No.
Oilseed Antinutritional Factors
1 Groundnut goitrogenic factors, trypsin inhibitors,
aflatoxins
2 Soybean trypsin inhibitors, saponin, legtins
3 Cottonseed Gossypol, malvalic acid
4 Rapeseed goitrogenic factors, thioglucosides
5 Linseed cyanogens compounds
6 Sesame oxalates
7 Sal tannins
8 Mowrah tannins, sponins
9 Castor ricin, allergens, ricinin
OILSEED: Source of Oil and protein (Meal).
AIM: To get high quality oil with best possible yield and to obtain oil meal
of highest possible value.
Oil Meal: Rich source of proteins and is used for human consumption,
animal feed and as farm yard manure. Use is restricted due to toxic
substances or anti nutritional factors. Anti nutrients left in oils gets removed
during the various refining stages. Most of them are left in cake, making it
nonedible.
Sr.
No.
Antinutritional
Factors
Effect
1 Lectins Affinity for sugar molecules. Attaches with sugar
moieties of cell membrane and interfere with the
absorption of nutrients across the intestinal wall.
2 Trypsin Inhibitor Inhibits enzyme activities of chymotrypsin,
plasmin
3 Goitrogens Inhibits uptake of iodine leading the deficiency of
iodine in thyroid and eventually produces goiter.
4 Tannins 8 – 10% in Sal meal. Forms complex with proteins
and enzymes. Reacts with trypsin (digestive
enzyme), making it insoluble. If there is protein
deficiency in diet, it reacts with body protein and
retards growth. Detected by brown coloration of
peanut hull. Content increases towards maturity.
5 Gossypol Cottonseed kernel contains 1.3% gossypol. Affects
color and nutritional properties of oil and meal.
Amino group of lysine reacts with –CHO group of
gossypol, making lysine unavailable during
digestion. Toxic to monogastri animals but not for
ruminants unless large amount is taken.
6 Aflatoxins Produced by Aspergillus flavus group of fungi. 4
types of aftatoxins are produced B1, B2, G1, G2. B1
is most toxic. Target organ is liver (hepatic
troubles) but may affect pancreas, kidney and
heart.
7 Phytic Acid Rapeseed flour contains 5% phytic acid. Phytate is
responsible for reducing mineral bioavailability.
Zn binds phytic acid and protein and decreases
solubility. Also binds trypsin, pepsin etc forming
insoluble complexes. During protein hydrolysis
phytate forms peptide phytate complexs those are
insoluble decreases solubility and availability of
nitrogen. Phytate reduces rate of starch digestion.
8 Glucosinolates Nontoxic if completely digested. In presence of
myrosinase produces glucose and isothiocyante.
Isothiocyante have goitrogenic properties, which
enlarges thyroid.
9 Ricin Occurs at a level of 1.5% in castor meal.
Extremely toxic and affects capillary blood
circulation in various organs like liver, spleen and
bone marrow. Quantity in milligrams can cause
poisoning and could be lethal.
10 Allergens Is nothing but chlorogenic acid. Can cause
omitting, diarrhea and fever.
11 Saponins Have surface active properties (Surface tension of
1% solution is 28 dynes/cm). Irritant to mucosal
membrane leads to gradual destruction of cells.
12 Estrogens Found in soybean. Consists of three isoflavons at
2.6 % level. Exist primarily as glucosides. These
are growth inhibitors.
Protein Recovery from Oilseed
Safflower seed Dehulling Hulls
Kernels
Prepressing and Solvent Extraction Oil
Defatted Meal Grind and Sieve Flour
Aqueous isoelectric pH adjustment, Membrane Processing and Drying
Washing and Drying
Protein Isolates
Coconut
Shell Remove Shell Coconut water
Meat Clean and Grind
Heating and Pressing
Coconut Milk
Oil Filter and Centrifuge
Vacuum Drying
Residue (Protein Concentrate)
Isoelectric pH adustment
Protein Isolates
Peanut
Hull Remove Hull Kernel Prepress and Sol. Extn Oil
Meal
Grind and Extract with dil. Alkali (pH = 8) and Filter
Liquid Extract Insoluble Residue
Adjust pH = 4 and Centrifuge Wash, Filter and Dry
Whey Protein Isolate Recovery of Insol. Protein
Detoxification of Meal
Castor Meal
Castor Meal
Steam
heating
at 15 psi
Toxicity decreases
to 1/2000th
of
original
Moist cooking with
2% NaOH/ Ca(OH)2
and 10% HCHO
Toxins and
allergins are
destroyed
Salseed and MowrahMeal
SoyMeal
Salseed/
Mowrah Meal
Treat with
30% aq.
Acetone
Dry the residue
Treat with 0.1%
NaOH overnight and
filter
Dry the
residue
Soymeal
Soxhlet
extraction by
ethanol
Saponins are
removed by
extraction