answers for quiz
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ANSWERS FOR QUIZ. Set-1. Answers to Bacterial gel. 1.760 2. 245 3. 16 spots 4.142/760 x 100 i.e.18.68% 5. 71-26 = 45. 6. 20 spots 7. 20/760 x 100 i.e.2.63% 8. 47-12 = 35. 9.(i) Spot ID: 2082 pI : ~4.53 Mol. weight : 24.9 kDal. - PowerPoint PPT PresentationTRANSCRIPT
ANSWERS FOR QUIZ
1.7602. 2453. 16 spots 4.142/760 x 100 i.e.18.68%5. 71-26 = 45.6. 20 spots7. 20/760 x 100 i.e.2.63%8. 47-12 = 35.9.(i) Spot ID: 2082 pI : ~4.53 Mol. weight : 24.9 kDal
(ii) Spot ID: 1999 pI : ~4.90 Mol. weight : 75.2 kDal
Set-1. Answers to Bacterial gel
Supportive material
Project Name Bacterial sample 2D-Gels for Analysis.Project Creation Date ………………Author …………………Description Bacterial sample Control vs Drug treated
GroupsControl(1 image)780spots transferred from fused image96 spots cancelled
Drug Treated(1 image)780 spots transferred from fused image96 spots cancelled
Vector Matching :245
1. Project Summary:
4.00 4.25 4.50 4.75 5.00 5.25 5.50 5.75 6.00 6.25 6.50 6.75 7.00
80
75
70
65
60
55
50
45
40
35
30
25
20
15
10
5
pI index
Mol
ecul
ar w
eigh
t (kD
al)
3.Figure showing Molecular weight to pI
85
pI : ~4.53 Mol. weight : 24.9 kDal
pI : ~4.90 Mol. weight : 75.2 kDal
No.
of s
pots
sho
win
g up
regu
latio
n
Up regulation
No. of folds(and above) No.of spots
2 142
4 71
6 36
8 26
10 16
12 12
15 10
4.1.Differential expression : Up regulation
Up regulationx Folds or above
No.
of s
pots
sho
win
g up
regu
latio
n
Down regulation
No. of folds(and above) No.of spots
2 174
4 47
6 20
8 12
10 8
12 4
15 1
Down regulationx Folds or above
4.2.Differential expression : Down regulation
1. 349 spots2. 1093. 16 spots4. 69/349 x 100 i.e.19.77%5. 33-16 = 17 6. 28 spots7. 48/349 x 100 i.e.13.75 %8. 83-28 = 559.(i) Spot ID: 2082 pI : 5.28 Mol. weight : 44.8 kDal
(ii) Spot ID: 1999 pI : ~5.26 Mol. weight : 65.2 kDal
Set -1. Answer of Serum sample
Supportive material
Project Name Serum sample 2D-Gels for Analysis.Project Creation Date …………………………………Author ……………….Description Healthy vs diseased patient
GroupsControl(1 image)Control - Healthy person349spots transferred from fused image97 spots cancelled
Diseased (1 image)P.falciparum diseased serum sample349 spots transferred from fused image97 spots cancelled
Vector Matching : 109
Project Summary:
4.00 4.25 4.50 4.75 5.00 5.25 5.50 5.75 6.00 6.25 6.50 6.75 7.00
80
75
70
65
60
55
50
45
40
35
30
25
20
15
10
5
pI index
Mol
ecul
ar w
eigh
t (kD
al)
85
pI : 5.28 Mol. weight : 44.8 kDal
pI : 5.26 Mol. weight : 65.2 kDal
3.Figure showing Molecular weight to pI
No.
of s
pots
sho
win
g up
regu
latio
n
Up regulation No. of folds(and above) No.of spots
2 693 474 395 338 21
10 1615 1520 1025 850 375 1
Up regulationx Folds or above
Differential expression : Up regulation
Down RegulationNo. of folds(and above) No. of spots
2 83
3 48
4 37
5 28
7 13
10 11
15 5
30 2
No.
of s
pots
sho
win
g up
regu
latio
n
Down regulationx Folds or above
4.2.Differential expression : Down regulation