Corporate Information

Board of Directors (As on 1st October, 2010) Dr. Suman Preet Singh Khanuja Chief Mentor & Director

Mr. Shreenarayan Agrawal Chief Operator & Director

Mrs. Meena Agrawal Director - Corporate Social Responsibility

Mr. Nandan Agrawal Director - Business Development

Head Office 19-A Sita Estate, Aziz Baug

Mahul Road, Chembur

Mumbai 400074, India

Phone: +91 22 25545244

Fax: +91 22 25545864

Research Centre Knowledge Centre D/365, Nandan Impex Building, 101, Park View Apartments,

MIDC Turbhe, Building No.II,

Navi Mumbai 400705, Mandi Village Road, Mehrauli,

Maharashtra, India New Delhi 110030, India

Phone: +91 22 27614451 Phone: +91 11 26804923

Fax: +91 22 27614451 Fax: +91 11 26804923

Website www.nutrahelix.com

Our Values

A word from Chief Mentor…

“The beginning of knowledge is the discovery of something we do not understand’’, was stated by the famous American author Frank Herbert, well known for his imagination in science. Today it seems true that food is something that we do not know well from the appropriateness perspective and hence it needs to be rediscovered. NutraHelix represents a unique industrial platform that was founded to create a discovery niche for future foods that would be able to enhance quality of life. That is why the mission slogan ‘making health sense in foods’ defines it. We began humbly through a science and business partnership in Mumbai

towards the end of 2009 as the foundation for Research Centre and also began the New Year with Knowledge Centre emerging with the ringing bells of 2010. The state-of-art laboratories at the Research Centre, Mumbai were launched on 16th February, 2011 by Dr. V Prakash, the torch bearer of food technology in the country and globe. NutraHelix Biotech Pvt. Ltd. has been initiated as the translational research venture aiming to learn from nature about nature for creation of knowledge based natural ingredients for functional foods with thorough scientific validation. It will not be inappropriate to say that the second ‘Green Revolution’ will happen only through quality of food and not by quantities. Linking agriculture with health sector is feasible when a strategic value chain is planned for meeting the nutritional requirements of life by identifying the essential health requirements of human body. NutraHelix has taken care of this aspect holistically while creating the infrastructure along with matching and competent scientific expertise. The projects that have begun, along with those planned for immediate future cover the aspects of bioprospecting, synbiotics, high value lipids, proteins and carbohydrates, food matrices, functional foods, nutraceutical and bioactive phytochemicals, enzymes, polysaccharides, biomolecules, food quality and safety standards. The skilled scientific team has blend of young and experienced members with sound training in field of agriculture, food science, microbiology, molecular biology, pharmaceutical science, nutrition, chemical engineering, formulation, product development and processing. Further to strengthen the ability of the organization, the management is represented by the competent Board of Directors namely Mr. Shreenarayan Agrawal (Industrial Business), Mrs. Meena Agrawal (Corporate Social Responsibility), Mr. Nandan Agrawal (Chemical Engineer with MBA) and lastly me (Science and Technology) as the base. The whole team and organization is committed on its mission of enhancing quality of life through science. This is the formative year but significant progress is visible from the first Annual Report itself which is an indicator that we will be the change that everyone wants to see in the world for health through foods and nature. With grateful thanks to all, I am happy to place the very first progress report to our catalysts, collaborators, promoters and clients for their knowledge inputs towards the build-up of NutraHelix

Dr. Suman Preet Singh Khanuja

Acknowledgement

Dr. V Prakash, Former Director, Central Food Technological Research

Institute (CFTRI/CSIR), Mysore, India

Director and Team, Indian Agricultural Research Institute (IARI/ICAR), New

Delhi, India

Director and Team, Central Institute of Post Harvest Engineering &

Technology (CIPHET/ICAR), Ludhiana, India

President, Director and Team, Indian Institute of Advanced Research (IIAR),

Gandhinagar, India

Co-Directors and Team, Joint Genomic Center (JGC), Sofia, Bulgaria

Director and Team, Centre for Advanced Research & Training in Biosciences

(CARTiB), Mumbai, India

Chairman and Team, Nandan Group, Mumbai, India

CONTENTS

Executive Summary

Organizational structure

Company Expertise

Research and Technology Areas

Target Products

Scientific Expertise

Projects in operation

Projects planned for immediate future

Infrastructure

Specialized Facilities

Linkages

Publications

Expert Services

Participation in National and International Events

Lead Lectures and Keynote Addresses by Chief Mentor

NutraHelix in Pictures

Statement of Assets and Costs

Executive Summary

Nutraceuticals are technically defined as foods or food derived substances in

extracted form, which carry the capability or ingredients to provide medicinal and

health benefits to living beings. Nutraceuticals may range from isolated nutrients,

herbal products, dietary supplements and diets to engineered “designer” foods and

processed products such as cereals, soups and beverages. Doubtlessly, many of these

products possess pertinent physiological functions and valuable biological activities.

Nutraceuticals are now rapidly emerging through scientific research with such leads

being generated all over the world. In many cases nutraceuticals offer an advantage

over the synthetic drugs under development by the pharmaceuticals industry. These

food derived pharmacological activities are making possible clinical use and thus

helping in disease prevention as well as therapeutics in several ailments and health

disorders. With availability of vast Indian knowledge of local health traditions and

food habits of communities, targeted R&D on their biological validation and quality

creates a great scope for novel functional foods, nutraceuticals and health

supplements from crops, plants and microflora.

India has all the advantages and potential of becoming the nutraceutical hub with

a long lasting tradition of maintaining health by the intake of appropriate food and

nutrition. India is home to almost all kinds of plants ranging from tropical, sub-

tropical and temperate zone plants. Moreover, India is blessed with abundant herbs;

the extracts of many of these herbs have been standardised and tested clinically for

its quality and efficacy. These extracts form an integral part of nutraceuticals and

functional foods that are available in developed countries. Additionally, India has a

lot of other advantages like qualified human resources, world class R & D facilities

and varied raw material-aspects that give our country a leading edge. Further, the

advantage of knowledge based remedies gives India tremendous leads in finding

newer applications (because of Ayurveda). Ayurveda, the five thousand year old

ancient Indian health science have mentioned benefits of food for therapeutic

purpose. Documents clearly show that the medicinal benefits of food have been

explored for thousands of years. This scenario puts India an advantage of Science

driven novel foods for health.

In this backdrop, NutraHelix Biotech Pvt. Ltd. was incorporated on 21st

December, 2009 as a result of joint initiative by Dr. Suman Preet Singh Khanuja and

Mr. Shree Narayan Agrawal on a Science and Business partnership platform as a

unique start up industry in the nutraceutical sector. Dr. Khanuja being former

Director of Central Institute of Medicinal and Aromatic Plants (CIMAP/CSIR),

Lucknow, India brings almost three decades of R&D experience and technology

application in the field of agriculture, biotechnology and natural products with IP

management. Parallel to it, Mr. Agrawal carries with him a rich industrial experience

from manufacturing to training in petrochemical sector involving import-exports in

global scenario. Dr. Khanuja was requested to lead and mentor this venture as R&D

based industry for creation of novel products, patents (IP) and technologies in the

nutraceutical and functional food sector to be translated into a real time business by

the expertise of Mr. Agrawal. Consequently, Dr. Khanuja has been leading

NutraHelix Biotech Pvt. Ltd. as its Chief Mentor with Mr. Agrawal leading as Chief

Operator since January, 2010. Nandan Group offered research labs available in their

building and hence research work on lead generation aspects began in February,

2010 itself. Further synergy was created by taking incubator space for food

microbiology work at Indian Agricultural Research Institute (IARI/ICAR), New

Delhi in October, 2010. This enhanced the pace of lead generation particularly pre-

and pro-biotics domains. Chief Mentor has been bringing visibility to the project by

delivering lead lectures in National Events like Nutra India Summit (2010, 2011);

Brainstorming sessions in National Academy of Agricultural Sciences (2010) and

Indian Horticultural Congress (2010) on the scope and opportunity aspects that have

been conceptualized by NutraHelix Biotech Pvt. Ltd. for the discovery platform on a

R2B mode (Research to Business).

Simultaneously, a state-of-art Research Centre with equipped labs was developed

on a leased out space covering more than 3000 square feet area at Turbhe in Navi

Mumbai. Launching of full-fledged Research Centre took place during 6th

Nutra

India Summit on 16th

February, 2011 by Dr. V. Prakash (then Director of Central

Food Technological Research Institute, CFTRI/CSIR, India). Today, NutraHelix

Biotech Pvt. Ltd. has full Research Centre with laboratories in Navi Mumbai and

Knowledge Centre in New Delhi. It is now developing Processing set-up for

translating the research leads from laboratories into manufacturing arena. This

venture has been an example of unique combination of Knowledge equity (Science)

and Financial equity (Business) on platform.

NutraHelix Biotech Pvt. Ltd. aims to develop well validated technologies for

functional foods and nutraceuticals as the sustainable IP and affordable healthcare

products, and its efforts in the area of research and development are directed to

achieve this goal. There is a dedicated Research team of scientists and associates for

developing nutraceuticals, which will have a tremendous potential for growth due to

the major factors such as ageing population, changing lifestyle, increasing medical

costs, high spending power, awareness of preventive medicine, and increasing

malnutrition led diseases. The Company has plans to further strengthen the R&D

base to cater to more healthcare needs and expanding niches in all nutraceutical

segments, both in domestic as well as international markets.

Organizational structure

Board of Directors (BOD)

Chief Operator Chief Mentor

Director-Business Development

Research & Development Process/Product Development Analytical Testing Business Development & Operations

Lead Scientist Lead Scientist Quality Manager Information Officer Business Manager

(Research Labs) (Process Technology) (Testing Labs) (Knowledge Centre) (Head Office)

Scientists & Associates Scientists & Associates Scientists & Associates

Industrial Microbiology Chemical engineering Analytical Chemist

Food Science & Technology Food &Nutrition Formulations/Pharmacy Assistants Assistants*

Biochemistry Post Harvest Technology* Dietician (F&N) (Office/Technical) (Office/Business)

Natural Prod Chemistry* Industrial Biotech* Microbiology (Med)*

Molecular Biology* Food Technology* Pharmacology/Toxicology*

Lab Technicians Plant Attendants Lab Technicians Liaison Staff Support Staff

Trainees/Fellows** Trainees/Assistants** Trainees/Assistants** Trainees** Trainees**

*To be appointed in due course, rests are already in place

**Partially filled, some vacancies to be filled later

Company Expertise

Agriculture, Food & Nutrition

Biotechnology (Plant/Animal/Microbial)

Biochemistry & Molecular Biology

Botany & Plant Sciences

Bio-Prospection & Conservation

Cell & Tissue Culture

Food Science & Technology

Medicinal & Aromatic Plants and Metabolomics

Food Processing & Product Development

Chemical Engineering & Pilot Plants

Research and Technology Areas

Food Science and Technology

Biochemistry and Nutrition

Food Quality Assays and Bio-testing

Biotechnology and Molecular Biology (Plant and Microbial)

Bio-prospecting & Conservation (Higher and Lower Plants)

Plant Metabolomics

Plant Tissue Culture & In vitro production

Microbiology and Algal technology

Botany and Pharmacognosy (Medicinal and Nutraceutical Plants)

Food ingredients (Nutraceuticals) and Supplements

Post-harvest technology

Food Processing and Product Development

Target Products

Novel Food Matrixes

Pre- & Pro-biotics

Dietary & Soluble Fibres

Antioxidants & Immunity Boosters

Lipids & Carbohydrates for Health

Millet Based Formulations

Gluten Free Formulations

Health Ingredients for Fast Foods

Health Beverage Formulations

Functional Foods for Liver & Cardiovascular Health

Metabolism Promoters & Gut Functioning Aids

Microbial & Enzymatic Supplements

Medicinal Plants based Phytoceuticals & Bioactives

Flavour & Fragrance of Nutraceutical Values

Scientific Expertise

Name Designation Specialization

Dr. Jai Shanker Arya

Lead Scientist Biotechnology

Industrial Microbiology

Plant bioactives and products

Bioreactors and Fermentation

Mr. Dharmendra Jain

Lead Scientist Process Technology (Pharma)

Chemical Engineering

Pilot and Manufacturing Plants

Fermentation Technology

Ms. Ashima Tewani*

Associate Scientist

Food Science and Technology

Food Microbiology

Food Analysis and Formulation

Mr. Yashaswi Singh

Scientist Trainee Plant Sciences

Biotechnology and Molecular Biology

Biochemistry

Mr. Anil Rambadan

Rajbhar

Research Associate Plant Taxonomy

Botany and Pharmacognosy

Biodiversity Survey

Herbaria and Repository

Ms. Sonali Avinash Dolas Research Associate Food Science and Nutrition

Dietetics

Home Science Applications (Foods)

Ms. Disha Gosalia Research Trainee Food Science and Nutrition

Dietetics

Home Science Applications (Foods)

Mr. Tirupati Babulal

Tatewar**

Associate Scientist Applied Chemistry

Analytical Instrumentation

Quality Testing (Natural products)

Mr. Jatinder Singh**

Technology

information Officer

(Scientist)

Biotechnology

Molecular Biology

Biochemical Technology

Pharmaceutical Sciences

Mr. Chandan Prakash** Technical Quality

Manager

(Scientist)

Business Administration

Pharmaceutical Sciences

Botany and Biotechnology

Formulation and Manufacturing

* Scientist Trainee till 28th

February, 2011 and Associate Scientist w.e.f. 1st

March, 2011

**To be shortly joining

Projects in operation

1. Bioprospection, documentation and repository of traditionally used plants

and foods

New research conducted among food scientists show that there is more to food

science than what was understood just a couple decades ago. Until just recently,

analysis of food was limited to the flavour of food (sensory taste and texture) and its

nutritional value (composition of carbohydrates, fats, proteins, water, vitamins and

minerals). However, there is growing evidence that other components of food may

play an integral role in the link between food and health. These chemical components

are derived from plant, food, and microbial sources, and provide medicinal benefits

valuable to long-term health. Examples of these nutraceutical chemicals include

probiotics, antioxidants, and phytochemicals. Nutraceutical products were considered

alternative medicine for many years. Nutraceuticals have become a more mainstream

supplement to the diet, now that research has begun to show evidence. India has all the

advantages of becoming a nutraceutical hub. India has a long lasting tradition of

maintaining health by the intake of appropriate food and nutrition. India is home to

almost all kinds of plants ranging from tropical, sub-tropical and temperate zone

plants. India is blessed with abundant herbs; the extracts of many of these herbs have

been standardised and tested clinically for its quality and efficacy. These extracts form

an integral part of nutraceuticals and functional foods that are available in developed

countries. Garcinia cambogia, Gymnema sylvestre, Phyllanthus embelica, Tribulus

terrestris, Momordica charantia, Curcuma longum, Green tea, Adathoda vasica,

Andrographis, Ginger, Tulsi, Phyllanthus niruri are quite a few of the original Indian

botanical products that are quite popular worldwide. These are used in a variety of

nutraceutical and functional food products. The world market for such products is ever

growing. Such products are being launched in India too. In fact, the use of these

products by the Indian consumers has been through the prescription channel. Some

examples of natural products used and exported from centuries in India are Aloe,

Green Tea, Withania, Chamomile, Ginger, Turmeric, Saffron, and Brahmi. Therefore,

this project aims at bioprospecting biodiversity and documenting the same while

creating real-time repository in and digital formats with following main objectives:

i. Collection of seeds and plant parts and reference food repository development

ii. Herbaria preparation and storage (real time and digitised)

iii. Monographs development and newsletter release

iv. Publications, Reports, Whitepapers, Brochure and Website/Digital Products

The collection has begun with weed species from road side, agricultural fields and

wild habitats without affecting the conservation status of the species. This will follow

their chemical/ biological analysis for food/ nutra values. The current status is as

follows:

Number of plant sources for which seeds are available: 70

Number of plant sources for which herbaria are available: 13

Number of plant sources for which both seeds and herbaria are available: 26

2. Novel synbiotic products development and process optimization.

Probiotics are referred to microflora consisting mainly the bacteria that help

maintain the natural balance of microflora in the human/ animal intestines. The normal

human digestive tract contains about 400 types of probiotic bacteria that as one of the

benefits reduce the growth of harmful bacteria and promote a healthy digestive

system. Experiments into the benefits of probiotic therapies suggest a range of

potentially beneficial medicinal uses for probiotics. An increased demand for nondairy

probiotic products comes from vegetarianism, milk cholesterol content, and lactose

intolerance. Therefore, the development of these products is a key research priority for

food design and a challenge for both industry and science sectors. Most of the

available products are some form of dairy, such as milk, ice cream, yogurt, cheese,

and frozen desserts, despite the continuously growth of the nondairy sector, with

products like soy-based drinks, fruit-based foods, and other cereal-based products.

In contrast prebiotics are non-digestible oligosaccharides particularly fructo-

oligosaccharides are known to stimulate the growth of endogenous and exogenous

bacteria into the gut. Combination of both probiotics and prebiotics known as

synbiotics represent the ultimate beneficial role into the host system. Still hardly any

non-dairy probiotic products are available which are stable on ambient temperature in

the market, it offers an R&D challenge for scientists to develop such stable non-dairy

products at ambient temperature. With this rationale, the project “Novel synbiotic

products development and process optimization” was taken up to develop stable

products with these main objectives:

i. Isolation and characterization of probiotic microflora from natural sources.

ii. Identification of prebiotic candidate materials from edible wastes of crops.

iii. Fermentation process for large scale probiotic cultures and stable synbiotic

formulations.

Around 52 strains were isolated from edible fermented food preparations involving

cereals, millets, fruits, vegetables and milk derivatives as follows:

S. No. Fermented preparation Number of Isolated

Probiotic strains

1 Fermented Dough (Cereals

and Millets)

27

2 Pickles (Fruits/ Vegetables) 4

3 Kimchi 3

4 Milk curd/Yoghurt 18

Biochemical Characterization of Isolates

S. No. Biochemical Characterization

Number of Positive Isolates

1 Intrinsic Antibiotic Resistance Pattern

(Ampicillin, Tetracycline, Rifampicin,

Ciprofloxacin, Gentamycin,

Erythromycin, Streptomycin,

Vancomycin, Penicillin, Nalidixic acid,

Linezoid, Kanamycin and Amoxicillin)

5 isolates were found to be resistant

against all tested antibiotics. All

resistant 5 isolates showed minimum

one positive character of probiotics

2 Bacteriocin production against E. coli, B.

Subtilis and P. aeruginosa

6 isolates showed antibacterial activity

against Escherichia coli

14 isolates showed antibacterial activity

against Bacillus subtilis

3 Lactose utilising capacity 11 isolates showed Lactose utilising

capacity

4 Proteolytic activity 25 isolates showed Proteolytic activity

5 Amylolytic activity 18 isolates showed Amylolytic activity

6 Lipolytic activity 20 isolates showed Lipolytic activity

3. Plant based sourcing for high value lipids, proteins and carbohydrates

Human beings evolved consuming a diet that contained approximately equal

amounts of omega-6 and omega-3 essential fatty acids. Over the past 100–150 years

there has been an enormous increase in the consumption of omega-6 fatty acids due to

the increased intake of vegetable oils from seeds of corn, sunflower, safflower, cotton

and soybeans. Today, in Western diets, the ratio of omega-6 to omega-3 fatty acids

ranges from 10 to 20:1 instead of the traditional range of 1–2:1. Studies indicate that a

high intake of omega-6 fatty acids shifts the physiologic state to one that is pro-

thrombotic and pro-aggregatory, characterized by increases in blood viscosity,

vasospasm, and vasoconstriction and decreases in bleeding time, whereas omega-3

fatty acids have anti-inflammatory, antithrombotic, anti-arrhythmic, hypolipidemic,

and vasodilatory properties. These beneficial effects of omega-3 fatty acids have been

shown in the secondary prevention of coronary heart disease and hypertension. Most

of the studies have been carried out with fish oils (eicosapentaenoic acid (EPA) and

docosahexaenoic acid (DHA)). However, α-linolenic acid (ALA), found in green leafy

vegetables, flaxseed, rapeseed, and walnuts, desaturates and elongates in the body to

EPA and DHA and by itself may have beneficial effects in health and in the control of

chronic diseases.

Mushrooms are a good source of easily absorbed, high quality vegetable protein,

containing more than most other vegetables. Mushrooms are a good source of the B

vitamins - niacin, riboflavin, thiamin, folate, vitamin B6, biotin and pantothenic acid.

Mushrooms are a good source of the minerals potassium, selenium, copper and

phosphorus. Mushrooms contain more potassium than most other vegetables and

fruits. Mushrooms are also one of the few dietary sources of Vitamin D. Therefore,

this project aims at plant based sourcing for high value lipids, proteins and

carbohydrates with the following main objectives:

i. Screening higher plants for Omega 3/6 rich oils as healthy fats

ii. Analysing weed plant seeds for essential amino acid balanced proteins

iii. Mushroom sourcing and processing for protein and carbohydrates supplements

A total of 70 weeds have been collected so far under bioprospection activity and

are being analyzed in this project. Crude fat content has been determined for all the 70

weeds by a semi-continuous method using Soxhlet apparatus but total protein content

has been determined for 14 weeds by Bradford and Lowry method using UV-Visible

spectrophotometer

0

5

10

15

20

25

30

35

0-10 % 10 - 20 % 20-30 % 30-40 40-50

Nu

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of

Pla

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Sou

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s

Crude Fat Content

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1

2

3

4

5

6

7

8

0-1 % 1-5 % 5-10 % 10-15%

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Total Protein Content

Initial leads for lipids and proteins (Quality aspect)

A. Lipids (Fatty acids)

S. No.

Fatty acid

Number of Plant sources

1 Eicosanoic acid 2

2 Linoleic acid 10

3 Linolenic acid 7

4 Oleic acid 10

5 Palmitic acid 9

6 Stearic acid 7

7 Arachidic acid 2

B. Protein (Amino acids)

S. No.

Amino acid

Number of Plant sources

1 Arginine 4

2 Histidine 2

3 Leucine 4

4 Isoleucine 3

5 Lysine 3

6 Methionine 3

7 Phenylalanine 4

8 Threonine 3

9 Cystine 1

10 Tyrosine 2

11 Valine 3

12 Asparagine 1

13 Ornithine 1

14 Canvanine 1

15 N-acetyl-L-djenkolic acid 1

4. Food matrix analysis and functional foods product development

Functional Foods which refer to processed foods containing ingredients that aid

specific bodily functions in addition to being nutritious offer great scope for

innovative products through R&D. Examples of functional foods include foods that

contain specific minerals, vitamins, fatty acids or dietary fibre, etc These can also be

the foods with added biologically active substances such as phytochemicals or other

antioxidants and even probiotics that have live beneficial cultures. The rise of

functional foods has occurred at the convergence of several critical factors, such as:

awareness of personal health deterioration, led by busy lifestyles with poor choices of

convenience foods and insufficient exercise; increased incidence and preferences of

self-medication; increased level of information from health authorities and media on

nutrition and the link between diet and health; scientific developments in nutrition

research; and a crowded and competitive food market, characterized by pressurized

margins. Food matrix is a carrier medium of nutrients/bioactive components/synbiotic

preparations and may also be a food in itself and therefore forms the core of any

functional food. With the aim to create dynamic platform of choices for food matrix,

the objective of this project aims for:

i. Screening and identification of new food matrices from millets and vegetable.

ii. Formulating functional foods by nutritional enrichment and balance.

iii. Process development and optimization for designer foods for common food

allergy suffering population

With respect to this, different nutritionally superior millets like jowar, bajra and

ragi in combination with cereals like rice and wheat and some horticultural biomass

categories are being explored to develop food matrices in the form of cookies, pasta

and noodles. The food matrices will be supplemented with bioactive components form

fruits and vegetable like Garcinia indica, Moringa oleifera, etc for its value addition

and further it will be explored as a carrier for probiotics. The prepared functional food

matrix will then be subjected to sensory, chemical and shelf life evaluation studies at

ambient temperature and conditions. The project also intends to develop and optimize

process for development of designer foods for the ailing population, i.e., gluten free

formulations for the gluten intolerant population, antioxidant and immunity boosting

functional foods for the immuno-compromised section of the society, etc. Initial leads

in such matrices and ingredient combinations are leading to value additions on cookie,

cracker, fruit bar and pasta formats as summarized below:

Category

Nutritional Value Addition

Cookie 1. Supplementation with a source of

bio-available vitamins and minerals which are the co-factors of

enzymes and co-enzymes

approximately 20 types of amino acids, 46 types of Anti-oxidants and

36 anti-inflammatory compounds

Vitamin A, B2, B3, B6, B9, B12, C, E, K, Calcium, Iron, Magnesium,

Phosphorus, Potassium, Sodium, Zinc

2. Providing a good source of calcium, manganese and iron.

3. Availability of two unique types of healing components: monoterpenes,

including carvone, limonene, and anethofuran; and flavonoids, including

kaempferol and vicenin.

4. Garlic advantage with Manganese, Vitamin B6, Vitamin C, Tryptophan,

Selenium, calcium, Phosphorus, Vitamin B1, Copper, Protein

5. Ginger flavour with contains Potassium, Magnesium, Copper, Manganese,

Vitamin B6

6. Selected seeds which are source of the minerals copper and manganese and

dietary fiber with monounsaturated fats have been included

7. Fruit sources for Vitamin A, Vitamin C, Thiamine, Riboflavin and Niacin have

been utilized

Cracker 1. Garlic advantage with Manganese, Vitamin B6, Vitamin C, Tryptophan,

Selenium, calcium, Phosphorus, Vitamin B1, Copper, Protein

Fruit Bar 1. Amaranthus as a very good source of vitamins including vitamin A, vitamin K,

vitamin B6, vitamin C, riboflavin, and folate, and dietary minerals including

calcium, iron, magnesium, phosphorus, potassium, zinc, copper, and

manganese

2. Plums as very good source of vitamin C, vitamin A, vitamin B2, potassium

and dietary fiber

3. Peach has potassium and vitamin C

Pasta 1. Millet included as good source of the minerals phosphorus, manganese and

magnesium

2. A refined cereal strips providing 66% fiber, 62% folate, 92% selenium and

99% phytochemicals that are essential for nutritional value

Projects planned for immediate future

1. Nutraceutical and bioactive phytochemicals from horticultural sources as

food ingredients and supplements

Beyond being the source of nutrients, foods, particularly derived from plant

sources, are a rich source of bioactive phytochemicals which are important for health

care. Research trends during the past 2-3 decades indicate that these phytochemicals

have an important role in preventing chronic diseases like cancer, diabetes, coronary

heart disease and hyper-cholesterolaemia. The major classes of phytochemicals with

disease-preventing functions are dietary fibre, antioxidants, detoxifying agents,

immunity-potentiating agents and neuropharmacological agents. Each class of these

functional agents consists of a wide range of chemicals with distinct biological

activities. For example, antioxidant function is exhibited by some nutrients, such as

vitamin E, vitamin C and provitamin A. Other phytochemicals that have antioxidant

properties are carotenoids, phenolic compounds, flavonoids and isothiocyanates. Some

of these phytochemicals show more than one functions. Foods rich in these chemicals

and exhibiting disease-protecting potential are called functional foods. Indian habitual

diets, which are based predominantly on plant foods like cereals, pulses, oils and

spices, are all good sources of these classes of phytochemicals, particularly dietary

fibre, vitamin E, carotenoids and phenolic compounds. Considering these points, this

project is designed with following objectives:

i. Analysis of horticultural crops, harvests and their wastes for antioxidants,

polyphenol and vitamin/micronutrient contents.

ii. Bioactive fractional component identification in fruits and vegetables to be

utilized as ingredients of foods for specific metabolic requirement.

iii. Process optimization and product development as food supplements and

functional foods

The project therefore intends to explore and analyze intelligently short-listed

horticultural crops like Bael, Garcinia, Moringa, Green tea, etc for their antioxidant,

polyphenol and vitamin/micronutrient content. The bioactive component from

different horticulture crops will be identified and subject to extraction and purification

for its utilization as ingredients of food supplements. The project also focuses on the

development and optimization of process for development of nutraceutical product.

With respect to this, till date different collections of Garcinia have been explored and

identified with respect to geographical locations. The main bioactive component in

Garcina, i.e. Hydroxy citric acid (HCA) primary useful for anti-obesity agent, has

been extracted and purified by different extraction method. A method for estimation of

HCA by TLC and colorimetric assay is under development and this will assist in

identification of Garcinia species with higher HCA concentration. Encapsulation of

bioactive component of fruit juices has been carried out with different mucilage in

spray dryer by optimizing different ratio of mucilage to juice concentrate.

2. Algal, fungal and microbial sourcing for enzymes, polysaccharides and

biomolecules

Recently, micro algae are emerging as a novel and renewable source of

biologically active compounds and nutritionally important macromolecules such as

Proteins, Carbohydrates, Lipids, Enzymes, Polysaccharides, Polyphenols,

Carotenoids, Vitamins and Minerals. However, the occurrence of bioactive

compounds in algal sources is less documented than that in higher plants. Therefore

micro-algae have a potential to explore the biologically active compounds which have

nutritional value and used as nutrient and micronutrients ultimately in the form of

functional food. As per the reported data Spirulina, Nostoc, Chlorella and Anabaena

have been exploited for nutritional and health values. Therefore it is needed to

explore the novel micro algae species from the natural sources which contain

biologically active compounds and useful nutritional macromolecules. Therefore the

project was taken up under these targeted objectives:

i. Screening of microalgae for nutritionally important proteins/enzymes and

carbohydrates

ii. Identification of new sources for oligosaccharides and lipids

iii. Biomolecules identification as nutraceuticals

Initially in a pilot study, 50 water samples were collected for Algae from water

bodies in Mumbai and Thane. Culturable algae found in only 6 water samples out of

random 15 water samples. These consisted of Chlorella colony, Oedogonium sps.,

Oedogonim like, Scenedesmus sps., Closterium sps., Microcystis sps. and Unidentified

sps. Simultaneous efforts were now on for survey and collection for lower plants with

focus on algae, mushroom and lichens towards nutraceutical value.

3. Food testing SOPs and safety standards diagnostics and QS

With rise of India as a resource hub of medicinal and nutraceutical plants and

establishment of FSSAI, it is not only desirable but mandatory requirement to validate

quality of functional foods and supplements in nutraceutical sector. The project

comprises of different analytical testing methods for the biochemical and thermal

analysis of different ingredients, food supplements and functional foods for quality

parameters from raw material and food products. The analytical methods involved

spectroscopic, chromatographic, titrimetric, gravimetric, and extraction techniques.

The analytical methods need optimization for extractions process, separation

techniques and quantification of food ingredients from crude materials. Also, the

determination of water quality parameters by the biochemical method is also

important for food manufacturing industries. The different SAPs and SOPs for the

analysis are under optimization/development. Project involves following objectives as

follow:

i. Development of analytical method for essential food ingredients such as;

Spectrophotometric method for protein, total carbohydrate,

hydroxycitric acid (HCA) analysis

Total fat by Soxhlet apparatus and fatty acid profile by GC

Sugar profile by HPLC

Polyphenols, carotenes, anthocyanins, and plant pigments etc. by

HPLC

Proximate analysis (ash, moisture, nitrogen etc.).

Water quality analysis for total dissolved solid, minerals, metals,

biochemical oxygen demand (BOD) and chemical oxygen demand

(COD)

Minerals and metals content by AAS/ICP

ii. Rapid diagnostics method for toxicity and adulteration analysis including

heavy metals and residues

iii. Microbial load and safety assurance analysis

Under these objectives, rapid spectrophotometric methods for total protein, total

carbohydrate, and hydroxycitric acid have been developed. The extraction process for

the protein, hydroxycitric acid, acidity, cholesterols, and nitrate and carotene from

solid/liquid food matrix is under optimization. The aim is to get NABL accreditation

of biological and chemical testing labs and be the analytical hub for nutraceutical food

testing as the recognized testing lab of Ministry of Food Processing.

Infrastructure

Research Centre

A. Research and Development

1. Plant Tissue Culture (Culture Handling)

2. Plant Tissue Culture (Growth Room)

3. Formulation Development (Ingredients)

4. Microbial Biotechnology (Fermentation and Culturing)

5. Media & Chemicals Handling (Sterilizers and Fumehood)

6. Molecular Biology (DNA, Protein, Lipid and Carbohydrate)

7. Fine Chemicals & Biologicals (Cryo- and Cold-storage)

8. Aseptic Room (Tissues and Cell Handling)

9. Herbarium (Plant collections)

10. Seed Repository (Crops and Weeds)

11. Crude Drug and Food materials repository

12. Bioinformatics (Database development and mining)

B. Analysis (Quality Testing)

1. Botany & Pharmacognosy (Analytical)

2. Bioprospection and Bioactivity Testing

3. Analytical Testing (Food and Chemical Testing Instrumentation)

4. Nutrition & Biochemistry (Analysis and method development)

5. Food Microbiology (Microbial Testing)

6. Food Analysis and Recipe Development

C. Processing (Bench scale - R&D)

1. Food Processing (Matrix and Products development)

2. Drying facility (Spray Dryer, Vacuum Dryer, etc.)

3. Baking and Steam processing

4. Oil expeller and extraction

5. Single screw extrusion for pasta and related products

6. Microencapsulation

7. Fruit processing (juices and pulps)

8. Cereal and pulse flours and dough making

Knowledge Centre

A. Learning through Resources

1. Library (Publications, Reports, Whitepapers, Brochure and Website/Digital Products)

2. Internet equipped computer systems

B. Facilitating through Technology

1. In-silico Research

2. Administration

C. Networking

1. Interaction zone

Specialized Facilities

In Operation

Analytical Instrumentation

Growth & Culture Rooms

Sterilization, Autoclaves & Quality Water

Chemical Handling Fume-hoods

Waste Recycling

Chemical Storage & Handling

Under Development

Cold Rooms & Conditioned Storage

Aseptic Clean Rooms/Labs

Controlled Condition Incubation

Biosafety Cabins

Dark Room & Photography

Distillation & Extraction

Incinerator & Decontamination

Linkages

1. Indian Agricultural Research Institute (IARI), New Delhi, India

NutraHelix Biotech Pvt Ltd. is a Corporate Member of Zonal Technology

Management and Business Planning and Development Unit, IARI and had also been

its business incubatee in the area of Nutraceuticals from October 1, 2010 to April 15,

2011.

2. Central Institute of Post Harvest Engineering & Technology (CIPHET),

Ludhiana, India

NutraHelix Biotech Pvt. Ltd. is registered with CIPHET for getting advisory

consultancy services and technologies on Post Harvest Agriculture and Processing of

Foods because CIPHET is a renowned institute and undertake lead researches in the

area of the post-harvest engineering and technology appropriate to agricultural

production catchment and agro-industries.

3. Indian Institute of Advanced Research (IIAR), Gandhinagar, India

NutraHelix Biotech Pvt Ltd. is collaborated with IIAR to conduct the applied and

analytical research in the frontier areas of science and to find application of the

research results for the benefit of mankind through nutraceutical products for

preventive healthcare. The two organizations are also willing and able to jointly carry

out technology development on the outcome research leads for industrial application

in food technology and products. This agreement describes those activities of interest

and priority areas that both signees have identified to focus in their collaboration, as

follows:

Special aim of the collaboration will be to identify novel plant sources for

Omega 3 and 6 fatty acid containing oil seeds to replace the fish oil need in

functional foods.

The second specific area identified is to develop various Aloe vera based safe

edible/skin care products ranging from juice/concentrates/gels/powders and

formulations.

The third area is of bioprospection to explore the new plant sources for

essential vitamins, amino acids and micronutrients and carry out research for

sustainable utilization of these resources.

4. Centre for Advanced Research & Training in Biosciences (CARTiB), Mumbai,

India

Both NutraHelix Biotech Pvt. Ltd. and CARTiBs have recognized their

complementary partnership feasible because of the common interest to carry out

research, offer skill development training and take up technology development in the

areas of "Bio-Science" and "Natural Products" leading to their use in the fields of

biotechnology, pharmaceuticals, health & nutrition, industrial applications, agriculture

and environment. Both organizations express their willingness to carry out

collaborative research in specific areas that will be identified time to time through

mutual discussion and consent on time bound projects mode.

5. Joint Genomic Center (JGC), Sofia, Bulgaria

NutraHelix Biotech Pvt Ltd and JGC have agreed to work together to advance the

scientific research through approaches intended to foster and stimulate additional

scientific exchange and collaboration between individual scientists and research

institutions in both countries. Jointly, they will identify broad areas of mutual interest,

and general activities that could be carried out in support of their shared priorities and

objectives. This agreement establishes the intent between NutraHelix Biotech Pvt Ltd

and JGC to work in a collaborative relationship for research and business activities of

interest and priority that both signees wish to focus are the following:

Priority to be given to the technology development through research related to

identification, characterization, studying of the properties, food ingredients and

health properties in traditional Bulgarian and Indian products, which play a

major role in maintaining human health.

Evolve technologies for world class safe and efficacious products of nutrition

using food/medicinal/cosmetic routes after thorough scientific validation

utilizing modern tools of molecular biology and biotechnology for biological

activity and toxicity/safety data generation.

Development of processes and products in functional foods and nutraceuticals

sectors the target of R&D and/or technology with business development.

Exchange of human resource skills, expertise, research materials and

information on specific topics of mutual interest and Identifying each other as

collaborators through their websites and print materials

Jointly prepare and submit technology based project schemes for international

support and commercialization routes

Publications

Monographs in Preparation

Aegle marmelos (Bael)

Aloe vera related species (Ghrita-kumari)

Amaranthus species (Ramdana)

Avena sativa L (Oats)

Bacopa monnieri (Brahmi)

Boerhavia diffusa (Punarnava)

Centella asiatica (Mandukparni)

Curcuma longa (Turmeric)

Euryale ferox (Makhana)

Fagopyrum esculentum (Buck wheat)

Garcinia species (Kokum)

Grewia species (Phalsa)

Juglans regia (Wallnut/Akhrot)

Linum usitatissimum L. (Flax)

Moringa oleifera (Drum Stick/Sehjan)

Ocimum species (Tulsi/Basil)

Phyllanthus emblica (Aonla)

Phyllanthus species (Bhui-amalki)

Psidium guajava (Guava)

Punica granatum (Pomegranate/Anaar)

Rhododendron species (Rose tree)

Stevia rebaudiana (Stevia)

Tamarindus indica (Imli)

Zingiber officinale (Ginger)

Ziziphus jujube (Ber)

Research and Reference Publications

Khanuja SPS, Jhang T, Shasany AK (2010) Medicinal and aromatic plants: A

case example of evolving secondary metabolome and biochemical pathway

diversity. In: Nature at work: Ongoing saga of evolution, (Editor: V.P. Sharma)

1st Edition, National Academy of Sciences, India, Springer (India) Private

Limited, New Delhi 110002, pp 355-368

Suman P.S. Khanuja (2010) Review of the book: Plants Affecting Human

Mind (Psycho Active Plants) by SK Jain. In: Proc Indian Natn Sci Acad 76 (1):

53-54

Khanuja SPS, Shukla AK (2011) Human health and nutrition: Functional

foods. In: Horticulture to Horti-Business (Editors: KL Chadha, AK Singh, VB

Patel), Proceedings Book of the Fourth Indian Horticulture Congress held at

New Delhi during 18-21 November, 2010, Westville Publishing House, New

Delhi, pp 433-445

Expert Services

Research Process Outsourcing (Food & Nutrition)

Novel Functional Foods and Nutraceutical Products (B2B)

Project Mentoring: Agri-food Biotech Innovations & Processes

Product Testing/Quality Validation & Bioassays Services

KPO (Literature / Feasibility/Projects/Information)

Concept to Co-development of products with Nutra Industry

Participation in National and International Events and Workshops

Training on “Microbiological and Biochemical Techniques involving

Isolation, Purification and Characterization of Microorganisms” on October

18-November 17, 2010 at Indian Agricultural Research Institute (IARI), New

Delhi

HPCL Congress India on December 16-17, 2010 at Grand Hayatt Hotel,

Mumbai

Training on “Molecular Biology and Biochemical Techniques involving DNA

Methods, PCR Tools and Molecular Markers and Diagnostic Probing of

microorganisms and higher plants” on January 3 – February 2, 2011 at Indian

Agricultural Research Institute (IARI), New Delhi

6th

Nutra India Summit 2011 on February 16-18, 2011 at World trade centre,

Mumbai

Session on "Ayurceuticals" The Marriage of Traditional Knowledge and

Modern Science on February 15-18, 2011 at World Trade Centre, Mumbai

Committed Participations:

Pharma-Nutrition conference on April 18-20, 2011 at Amsterdam, The

Netherlands

Bangalore INDIA BIO on May 4-6, 2011 at Bangalore International Exhibition

Centre, Bangalore

ICAR-CII Industry Meet on May 23, 2011 at NASC Complex, New Delhi

3rd

International Conference on "Nutraceuticals, Functional Foods and Dietary

Supplements" on June 23, 2011 at Intercontinental – The Lalit, Mumbai

National Conference on Career and Research Trends in Food Processing on

June 24-25, 2011 at Indian Institute of Crop Processing Technology (IICPT),

Thanjavur, Tamil Nadu

7th

Food & Technology Expo – 2011 on July 29-31, 2011 at Pragati Maidan,

New Delhi

Lead Lectures and Keynote Addresses by Chief Mentor

1. Phytochemicals for Human Health Care - Lead Lecture by Dr SPS Khanuja in the

session "Role of Phytochemicals and Nutraceuticals in human nutrition and health" of

the International Conference on Role of Biomolecules in Food Security and Health

Improvement, on February 17-20, 2010 at BHU, Varanasi

2. Aromaceuticals - the way forward for opportunities in essential oils and aromatics -

Keynote Address by Dr SPS Khanuja in the first Technical Session of Asian Aroma

Ingredients Congress & Expo 2010 on May 6-9, 2010 at Hotel Leela, Gurgaon

3. Phytonutraceuticals: the molecules for health from plant metabolism -Public Lecture

delivered by Dr SPS Khanuja at Centre for Converging Technologies (CCT) for

students and faculty on August 6, 2010 at University of Rajasthan, Jaipur

4. Genes to genomes: the discovery path of biotechnology" – Academic Lecture

delivered by Dr SPS Khanuja for students and faculty of JNU on August 7, 2010 at

Jaipur National University, Jaipur

5. Plant metabolome based strategic research need for designing and breeding

nutraceutical crops – Theme Lecture by Dr SPS Khanuja in NAAS Brainstorming

Session on “Prioritization and value addition of nutritionally important crops” on

September 18, 2010 at National Academy of Agricultural Sciences, National

Agricultureal science Complex, New Delhi

6. 4th

Indian Horticulture Congress 2010-Invited Lecture delivered by Dr SPS Khanuja

Organized by NSFI (National Skill Foundation of India) and HIS (Horticultural

Society of India) on November 18-21, 2010 at National Physical Laboratory, New

Delhi

7. Revisiting horticulture as health sector catalyst through fruit genomes and metabolites

- Lecture by Dr. SPS Khanuja. in the 6th

Nutra India Summit 2011 on February 16-18,

2011 at World Trade Centre, Mumbai

NutraHelix in Pictures

Auspicious beginning of NutraHelix Research Centre on 6th Feb 2011

Launching of NutraHelix Research Centre at 6th Nutra India Summit 2011, World Trade Centre, Mumbai on 16th February 2011

NutraHelix Research Centre Laboratories, Turbhe, Navi Mumbai

Laboratories Contd..

Laboratories Contd..

NutraHelix Knowledge Centre, New Delhi

Statement of Assets and Costs

Certificate for cost of Fixed Assets owned by the company

Certificate for Fixed Assets taken on lease by the company

Certificate for Preoperative Expenses and Other Advances and

deposits

SANJAY SAMDANI & ASSOCIATESChartered Accountants

r-- ~- --

iCA"""\, Ii / II . j! I\ J

v, --_ ..... /

106-D, Trade Corner,Sakinaka, Mumbai 400 072

2: 022-28526152, Tele-fax:022-28520621Email: ssa@samdani.com

ToNutrahelix Biotech Pvt. Ltd.19-A, Sita Estate,AzizBaug, Mahul Road,Chembur,Mumbai 400074

Date: 03/08/2011

Sub : Certificates

Please fmd attached herewith following three certificates as requested by you forapplication and registration purpose.

1. SSA/ 11-12/Nutra 02 Certificate for Cost of Fixed Assets owned by the company2. SSAj11-12 / Nutra 03 Certificate for Fixed Assets take on lease by the company3. SSA/ 11-12/Nutra 04 Certificate for Preoperative Expenses and Other Advances

and deposits

We hope you will find the above in order.

~~.SANJAYSAMDANIProprietor

SANJAY SAMDANI & ASSOCIATESChartered Accountants

------------\(CA,lI _ i

I :\ /\\~- ---~

106-D,Trade Corner,Sakinaka, Mumbai 400 072

if: 022-28526152, Tele-fax:022-28520621Email: ssa@samdani.com

Cert. No,:- SSA/ 11-12/Nutra 02

CERTIFICATE

This is to certify that we have verified the books of accounts and other relevant

records and documents of NUTRAHELIX BIOTECH PVT. LTD having its registered office

as at 19A, Sita Estate, Aziz Baugh, Mahul Road, Chembur, Mumbai 400074 and based

on such verification and representation, we certify below the cost of Fixed Assets owned by the

Company as on 31.03.2011:

S.No, Fixed Assets Amount (Rs.l

1.

2.

3,

Lab Equipments

Computers

Office Equipments

1,76,390,00

2,00,851.00

11,243.00

Total 3,88,484.00

This certificate is issued at the request of Nutrahelix Biotech Pvt. Ltd for applicationand registration purpose.

For SANJAYSAMOANI& ASSOCIATESChartered AccountantsFirm Registration No. 109183W

~,

SANJAYSAMOANIProprietorM.No.: 041057Place: MumbaiDate: 05. tJg'U'1/

SANJAY SAMDANI & ASSOCIATESChartered Accountants

(CA··-----\i . ~,i [\ j

\,.._--j

106-D, Trade Corner,Sakinaka, Mumbai 400 072

if: 022-28526152, Te1e-fax:022-28520621Email: ssa@samdani.com

Cert. No.:- SSAI 11-12/Nutra 03

CERTIFICATE

This is to certify that we have verified the books of accounts and other relevant records and

documents of NUTRAHELIX BIOTECH PVT. LTD having its registered office as at 19A,

Sita Estate, Aziz Baugh, Mahul Road, Chembur, Mumbai 400074, and based on such

verification and representation, we certify the statement of Cost of Fixed Assets taken on lease

from Mis. Nandan Impex Private Ltd.as on 31.03.2011,which are as under:

S.No. Fixed Assets Amount (Rs.)

l. Lab Equipments 22,18,854.00

2. Computers 1,51,816.00

3. Office Equipments 68,33,720.00

4. Factory Building 38,99,42l.00

------------------Total 1,31,03,81l.00

=============This certificate is issued at the request of Nutrahelix Biotech Pvt. Ltd for applicationand registration purpose.

For SANJAYSAMOANI& ASSOCIATESChartered AccountantsFirm Registration No. 109183W

~~~

SANJAYSAMOANIProprietorM.No.: 041057Place: MumbaiDate : 03· 0 g .')-c I I

SANJAY SAMDANI & ASSOCIATESChartered Accountants

(rl\--"\Iv~(!\ ./'-".. -

106-D,Trade Corner,Sakinaka, Mumbai 400 072

if: 022-28526152, Tele-fax:022-28520621Email: ssa@samdani.com

Cert. No.:- SSA/ 11-12/Nutra 04CERTIFICATE

This is to certify that we have verified the books of accounts and other relevant records anddocuments of NUTRAHELIX BIOTECH PVT. LTD having its registered office as at 19A,Sita Estate, Aziz Baugh, Mahul Road, Chembur, Mumbai 400074 and based on suchverification and representation, we certify Pre-operative Expenses incurred and OtherAdvances & Deposits given by the Company as on 31.03.2011as under:

A)PR-EOPERA11VE EXPENSES:

S,No.1.2.3.4.5.6.7.8.9.10.11.12.13.14.

S.No.1.2.3.4.5.

.ParticularsSalary & Allowance to StaffStaff Welfare ExpensesConveyance & Travelling ExpensesVehicle ExpensesElectricity ExpensesConference ExpensesFees & Subscription ExpensesPostage & Telegram ExpensesOther Office & Admin. ExpensesBank ChargesPreliminary ExpensesLease RentLaboratory ExpensesRepair & Maintenance

BlaTHER ADVANCES& DEPOSITS

ParticularsAdvances against MachineryMVATCredit availableAdvances against expensesDeposit of VATStaff Advances

Total

Arnount(R3.}6,85,53G.tJO

28,089.0040,686.0077,985.0014,520.00

2,00,117.0058,876.2059,214.00

3,06,777.002,097.50

99,300.008,00,000.004,03,752.85

30,642.00

Amount(Rs.l4,27,500.00

69,643.0038,408.0025,000.004,000.00

Amc'untf.Rs.}

28,07,643.00

5,64,551.0033.72,194.00

This certificate is issued at the request of Nutrahelix Biotech Pvt. Ltd for applicationand registration purpose.

SANJAYSAMDANIProprietorM.No.: 041057Place: Mumbai