anastasia gorbunova, ma (university of arizona) modality instructor: dr. justin crowley
DESCRIPTION
MNTP2009: Optical Imaging Modality. Anastasia Gorbunova, MA (University of Arizona) Modality instructor: Dr. Justin Crowley Training Assistant: Corey Flynn. Visual masking with optical imaging of intrinsic signal, two-photon calcium imaging, and single unit recording. Visual Masking. - PowerPoint PPT PresentationTRANSCRIPT
Anastasia Gorbunova, MA (University of Arizona)Modality instructor: Dr. Justin CrowleyTraining Assistant: Corey Flynn
MNTP2009: Optical Imaging Modality
“Destructive interaction or interference among transient stimuli that are closely coupled in space or time”
R. Fox. (1998). In Handbook of Sensory Physiology. VIII. Perception.
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Forster & Davis (1984) J Exper Psych: Learning, Memory & Language
Optical imaging of intrinsic signal- wide spatial field- temporal resolutionslow hemodynamic dependentfaster light scattering properties of tissue
Single unit recordings - best temporal resolution Two-photon Calcium imaging
- distinct neurons, fine time course
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• Exposed visual cortex of a ferret (P80-130)
• Illumination by ~700 nm light
• Visual stimulation (4 degree bars)
• CCD camera (100 ms data frames)
• Computer digitization
8000 ms
Courtesy of David E. Whitney
Time
Stimulus 1
Stimulus 2
Blank stimulus 8.1 s
0.1 s
0.1 sInter-Stimulus Blank 3 s
ISB 3 s
ISB 3 s
8 s
8 s
1 Block
- =
100 ms 100 ms 100 ms
8 s 8 s 8 s
100 ms
8 s
100 ms
8 s
100 ms
8 s 1 frame: 500 ms
• Exposed visual cortex of a ferret (P80-130)
• Carbon fiber electrode• 20kHz signal digitization
H-B
H-B
V-B
H-V
V-H B H HV V
SQ
P
SQ
P
SN
P
SN
P B
V-H
H-V
V-B
H, horizontal grating; B, blank; V, vertical grating; SQP, square-wave plaid; SNP, sine-wave plaid
Oregon green BAPTA-1AM (labels neurons and astrocytes)
Sulphorhodamine-101 (labels astrocytes)
Microscope: Zeiss LSM 510 Meta NLO with MIRA900 Ultrafast Ti:Sapphire laser
20x NA1 objective
3 cells: ΔF/F for 700 frames normalized over 10 blocks
It is possible to study masking in animal models using simple visual stimuli
Very brief presentation of static gratings produces sufficient activation detectable by optical imaging of intrinsic signal
Orthogonal mask disrupts that activation
Dr. Justin CrowleyCorey FlynnDavid WhitneySruthi ChintakuntaAlberto Vazquez
Dr. Seong-Gi KimDr. Bill EddyTomika CohenRebecca Clark
Thank you!