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Supplementary Fig. 1

Supplementary Fig. 1 Schematic of bioinformatic data processing and database configuration.

Supplementary Fig. 2

Supplementary Fig. 2 Assessment of the CTAB cleanup protocol on the quality and quantity of DNA. (A) Results of NanoDrop and Qubit DNA quantification before and after CTAB clean up. (B) Photo and electrophoresis gels of eluted DNA from a pigmented melanoma before (left) and after CTAB cleanup (right). (C) Copy number plot derived from a whole genome sequence of a pigmented DNA samples following CTAB cleanup.

Supplementary Fig. 3

Supplementary Fig. 3 Example IGV images of matched tumour and normal genomes that can contribute to incorrect mutation detection using the simultaneous calling method. (A) Tumour variant call is just above our variant cut-off score and normal variant call is just below this cut-off score and contributes to a false positive mutation using the subtraction method. (B) Normal sample has little or no coverage while tumour sample has enough cover to call a variant.