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VIETNAM: Progress report Survey on AMU and AMR in aquaculture

Aquatic Animal Health Div., DAH. Email: tyts.cucthuy@gmail.comDang Thi Lua, RIA1. Email: danglua@ria1.org

FMM/RAS/298: Strengthening capacities, policies and national action plans on prudent and responsible use of antimicrobials in fisheries Workshop 2

in cooperation with Malaysia Department of Fisheries and INFOFISH7-9 August 2017, Kuala Lumpur, Malaysia

I. SURVEY DESIGN AND PLANII. FIELD SURVEYIII. SAMPLING AND TESTING

PRESENTATION OUTLINE

I. SURVEY OF AMU, AMR IN FRESHWATER FISH CULTURE

1.1. OBJECTIVE: To assess current status of AMU and AMR in tilapia and traditional fishes (freshwater fish) in Nam Sach and Tu Ky districts of Hai Duong province

1.2. ACTIVITIES• To investigate status of culture, disease and AMU for freshwater fish

production in Nam Sach and Tu Ky districts of Hai Duong province• Collect samples for testing of pathogens (Streptococcus spp. &

Aeromonas spp.) and test for AMR of the pathogens

1.3. DESIGN• Target on farms that have areas of at least 0.2 ha (the average size of

farms in Hai Duong province)• In each district, select purposely three key aquaculture communes of FW

fish• Using the principles and techniques of the epidemiological cross-section

study and random multi-stage sampling strategy

I. SURVEY DESIGN AND PLANII. FIELD SURVEYIII. SAMPLING AND TESTING

PRESENTATION OUTLINE

2.1. Field surveyUse random multi-stage sampling strategy in 4 following steps Step 1: Selection of survey location• Purposely select 3 freshwater farming communes in each district of

Nam Sach and Tu Ky districts from the list that was provided by local veterinary authorities:

- Tu Ky district: Tan Ky, An Thanh and Dai Dong communes- Nam Sach district: Quoc Tuan, Thai Tan and Nam Tan communes

Step 2: Farm selection• List all cultured farms of 6 selected communes and then randomly

select 10 farms which have area of more than 0.2 ha from each chosen communes to investigate

• Farms are coded consistently based on unique number: 2 initials of district name + 2 initials of commune name + order number of farm, eg. TKAT01

II. FIELD SURVEY ON AMU AND AMR IN FRESHWATER FISH CULTURE

• A survey will be carried out in Hai Duong province (northern Vietnam): KAP survey

• Key freshwater fish culture area• Nam Sach and Tu Ky districts were

selected

NORTH OF VIETNAM

2.1. Field survey (cont.)

Step 3: Conduct investigation

Key technicians of DAH and RIA 1 shall:• Provide a training for field investigators before implementing

survey

• Collaborate with leader key technical staff of provincial Sub-DFISH of Hai Duong to visit the selected farms and carry out face-to-face interview to collect relevant information based on the questionnaire

II. SURVEY PLAN ON AMU AND AMR IN FRESHWATER FISH CULTURE

2.1. Field survey (cont.)Step 4: Data summary and analysis• Collected data will be inputed into MS Access/Excel database• Descriptive analyses are carried to summarize the main features and identify variables for statistical analyses• Multivariate analyses will be carried out to determinate the odd ratios of AMU and associated factors

II. SURVEY PLAN ON AMU AND AMR IN FRESHWATER FISH CULTURE

I. SURVEY DESIGN AND PLANII. FIELD SURVEYIII. SAMPLING AND TESTING

PRESENTATION OUTLINE

3.1. Sampling and antimicrobial testingStep 1: During the KAP survey, fish samples will be collected, labeled, preserved and transported to RIA1’ lab for detecting the concerned pathogens and testing of AMRStep 2: Testing of samples

• Bacterial culture methods are used to detect Streptococcus spp. and Aeromonas spp. (these pathogens are the most common in causing diseases in freshwater fish in Vietnam)

• Use antimicrobial test to assess AMR of the above pathogens to the most common antimicrobials

Step 3: Summary of information and data analysis

III. SAMPLING AND TESTING FOR ANTIMICROBIAL SUSCEPTIBILITY

Testing contents Number of samples

Isolation and identification of Streptococcus caused diseases in tilapia and other freshwater fishes 60

Isolation and identification of Aeromonas caused diseases in tilapia and other freshwater fishes 60

Test for antibiotic resistance to Streptococcus with 14 antibiotics (Neomycin Sulphate; Doxycyline; Hydrochloride; Thiamphenicol flofenicol, Sulfadiazine (SD); Sulfamethoxazole (SMZ/TMP); Sulfadimidine (SM2); Sodium Sulfamonomethoxine (SMM-Na); Enrofloxacin; Flumequine; ...)

30

Test for antibiotic resistance to Aeromonas with 14 antibiotics (Neomycin Sulphate; Doxycyline; Hydrochloride; Thiamphenicol flofenicol, Sulfadiazine (SD); Sulfamethoxazole (SMZ/TMP); Sulfadimidine (SM2); Sodium Sulfamonomethoxine (SMM-Na); Enrofloxacin; Flumequine; ...)

30

3.2. Survey scheduleNo. Contents Starting

timeClosing

time1 Collect information

- Co-operating with Hai Duong sub-Dfish to collectinformation about farms, production and diseasestatus.- Discuss proposed implementing plan with RIA 1 andHai Duong sub DFish to agree the final surveillanceplan.

01/7/2017 15/7/2017

2 Prepare essential conditions before implementingsurvey- Develop survey questionnaire.- Select survey farms.- Training for investigators.- Contact with the People’s Committee of the selectedcommunes and related organizations and individuals tofinalize investigation plan.

15/7/2017 15/8/2017

3 Investigating and entering survey data 15/8/2017 25/8/20174 Summary and analysis of survey data 25/8/2017 28/8/20175 Reporting and closing survey 28/8/2017 30/8/2017

3.3. Survey questionnaire

Thank you for your attention!