transposable elements: insertion pattern and impact on gene expression evolution in hominids maria...
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Transposable elements: insertion pattern and impact on gene expression evolution in hominids
Maria Warnefors, Vini Pereira, Adam Eyre-Walker
University of Sussex, Brighton, United Kingdom
SMBE, Lyon, 2010
Species-specific transposable elements (TEs)
2933 insertions7786 insertions
~95% of these TEs are Alu, L1 or SVA elements
(Mills et al, Am J Hum Genet, 2006)
Images: Wikimedia Commons
Me Me Me Me
TEs can influence gene expression via several mechanisms
TEs provide transcription start sites (TSSs) for 30% of human transcripts (Faulkner et al, Nature Genetics, 2009)
Intronic L1 insertion reduces reporter gene expression in HeLa cells(Han et al, Nature, 2004)
TE silencing downregulates neighbouring genes in Arabidopsis(Hollister and Gaut, Genome Research, 2009)
Have TEs increased expression divergence (ED) between humans and chimpanzees?
Low ED
Have TEs increased expression divergence (ED) between humans and chimpanzees?
Low ED
High ED
Previous studies of TEs and expression divergence
20% of mouse/rat ED is due to TEs (Pereira et al, PLoS ONE, 2009)
Alu elements correlate with mouse/human ED, but the direction depends on the definition of ED (Urrutia et al, Genome Biology, 2008)
Materials and methods
Expression divergence was calculated for ~9000 genes based on expression in five tissues (data from Khaitovich et al, Science, 2005)
Expression divergence is the Euclidean distance between expression levels in one or more tissues
Species-specific TEs (Mills et al, Am J Hum Genet, 2006) were grouped according to insertion site:
UPSTREAM INTRONIC DOWNSTREAM
2 kb10 kb20 kb 2 kb 10 kb 20 kb
Species-specific TEs are associated with an increase in expression divergence
TE
No TE
Combined p = 0.024
Z transformation method (Whitlock, J Evol Biol, 2005)
ED
Do TEs cause the change in expression?
H. sapiens P. troglodytes M. mulatta
SPECIFICContribute to ED
SHAREDDo not contribute to ED
Images: Wikimedia Commons
Shared TEs are also associated with an increase in expression divergence
TE
No TE
SPECIFIC (p = 0.024)
SHARED (p = 0.00003)
ED
ED
Recent TE insertions did not cause an increase in expression divergence
Relative effect =EDSPECIFIC / EDNO SPECIFIC
EDSHARED / EDNO SHARED
Second approach: Human-macaque versus chimp-macaque expression divergence
H. sapiens P. troglodytes M. mulatta
Expression data:~3700 genes in the prefrontal cortex(Somel et al, PNAS, 2009) Images: Wikimedia Commons
Species-specific TEs have not caused increased ED
H/M
C/M
p = 0.32
p = 0.13
Do TEs act to increase transcript diversity?
New TE No new TE p value (M-W)
Human 2.7 2.3 2 x 10-16
Chimp 2.0 1.8 5 x 10-10
Do TEs act to increase transcript diversity?
Expected transcript div.
New TE No new TE p value (M-W)
Human 2.7 2.3 2 x 10-16
Chimp 2.0 1.8 5 x 10-10
No evidence that recently inserted TEs have contributed to hominid transcript diversity
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TE insertions are more common upstream than down-stream of genes
The upstream bias is just significant (p = 0.049).
Summary of ~9000 genes (from the Khaitovich dataset)
561 TEs
496 TEs
TE insertions are more common upstream than down-stream of genes
The upstream bias is just significant (p = 0.049).
TEs may accumulate upstream because they have a role in chromatin regulation. (Huda et al, Gene, 2009)
TEs may preferentially insert upstream (as known for Drosophila P elements)
Summary of ~9000 genes (from the Khaitovich dataset)
561 TEs
496 TEs
Hominid TEs are subject to insertion bias
TE upstream TE downstream
Active in germ line 255 181
Inactive in germ line 306 315
Only germ line expressed genes exhibit upstream bias (p = 0.003, 2test)
The enrichment of TEs in upstream sequences can be explained in terms of insertion bias and does not indicate a role for TEs in gene regulation
Expression state from eGenetics data (Kelso et al, Genome Research, 2003)
Summary
New TEs are correlated with, but not causal to, an increase in expression divergence between human and chimpanzee. (However, TEs may still contribute substantially to within-species variation).
We do not find evidence for a TE-induced increase in transcript diversity.
Excess of upstream insertions can be explained by neutral insertion bias.
To what extent do regulatory regions evolve within TEs, simply because TEs are so abundant?
Acknowledgements
Adam Eyre-Walker
Vini Pereira
John Brookfield
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