the protein kinase pstol1 from traditional rice confers tolerance of phosphorus deficiency...
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The protein kinase Pstol1 from traditional riceconfers tolerance of phosphorus deficiency
12-04-2015
Gamuyao et al., 2012. Nature
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Biological role
Phosphorus (P) is an essential macronutrient for plant growth
It is central to a majority of the molecular constituents required for the functioning of plant cells.
DNA; RNA; Proteins; Lipids; Sugars, ATP, ADP, and NADPH
It is indispensable in agricultural production
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The problem of phosphorus
Kochian Nature 488, 466–467 (2012) doi:10.1038/488466a
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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
Origin of the donor variety Kasalath
• aus-type variety
Aoosh (early)
This group of rice is famously known to tolerate difficult growing conditions
• Pup1
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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
Phosphorus uptake 1 (Pup1) candidate genes
OsPupK46-2
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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
Postl 1 is the major genetic determinant of P-deficiency tolerance
Phosphorus-Starvation Tolerance 1
OsPupK46-2
Nipponbare near-isogenic lines(NILs) -P conditions With or without P fertilizer
Which one is the major determinant of P-deficiencytolerance in Kasalath?
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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
Pstol1 is a functional protein kinase
in vitro phosphorylation assay
How Pstol1 involved with the P-efficient ?
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PSTOL1 overexpression enhances tolerance of P deficiency
R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
35S::PSTOL1
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Indica
Japonica
The larger root system also enhanced the uptake of other nutrients
R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
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How Pstol1 involved with the P-efficient?
By enhancing root growth?
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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
PSTOL1 is an enhancer of root growth and independent of phosphorus or soil-related conditions
100mM
10mM
Really ?
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Pstol1
IR64 transgenics
R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
RNA interference in Kasalath
PSTOL1 is an enhancer of root growth!
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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
How PSTOL1 enhance root growth?
The expression of PSTOL1 during root development
PSTOL1 is a regulator of early crown root development and root growth
Crown root Main root system
Crown Root Primordia
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Parenchymatic cells
vascular bundles
Emerging crown roots
R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
PSTOL1 putative downstream genes
23 genes related to root growth and stress response with constitutively higher or lower expression in the transgenic plants were identified
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R Gamuyao et al. Nature 488, 535-539 (2012) doi:10.1038/nature11346
PSTOL1 putative downstream genes
21 of these differentially expressed genes co-localize with QTLs related to drought tolerance and root growth
a positive regulator ofroot cell differentiation
delay leaf senescencein rice
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